Eiji Oomae
Last Updated :2023/09/06
- Affiliations, Positions
- Graduate School of Integrated Sciences for Life, Assistant Professor
- E-mail
- ohmae
hiroshima-u.ac.jp
Basic Information
Academic Degrees
- Doctor of Science, Hiroshima University
- Master of Agriculture, Nagoya University
Research Fields
- Biology;Biological Science;Biophysics
Research Keywords
- Function of enzymes
- Structure of enzymes
- High hydrostatic pressure
- Deep-sea enzymes
Educational Activity
Course in Charge
- 2023, Undergraduate Education, 1Term, Introduction to Chemistry A
- 2023, Undergraduate Education, 4Term, Advanced Chemistry
- 2023, Undergraduate Education, First Semester, Exercises in Physical Chemistry
- 2023, Undergraduate Education, 3Term, Bioinformatics
- 2023, Undergraduate Education, First Semester, Chemical Experiments I
- 2023, Undergraduate Education, Second Semester, Chemical Experiments II
- 2023, Graduate Education (Master's Program) , 2Term, Proteomics
- 2023, Graduate Education (Master's Program) , 2Term, Topical Seminar in Life Science A
- 2023, Graduate Education (Master's Program) , 4Term, Topical Seminar in Life Science B
Research Activities
Academic Papers
- Effects of pressure on enzyme function of Escherichia coli dihydrofolate reductase, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1784(7-8), 1115-1121, 2008
- Pressure dependence of the apparent specific volume of bovine serum albumin: Insight into the difference between isothermal and adiabatic compressibilities, BIOPHYSICAL CHEMISTRY, 144(1-2), 67-71, 200909
- Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase revealed by deletion mutants, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1804(4), 846-855, 201004
- Cloning and characterization of dihydrofolate reductases from deep-sea bacteria, JOURNAL OF BIOCHEMISTRY, 147(4), 591-599, 201004
- Comparative study on dihydrofolate reductases from Shewanella species living in deep-sea and ambient atmospheric-pressure environments, EXTREMOPHILES, 15(2), 165-175, 201103
- Pressure-dependent Activity of Dihydrofolate Reductase from a Deep-sea Bacterium \textit{Shewanella violacea} Strain DSS12, Chemistry Letters, 33(7), 798-799, 20040701
- Mutational analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase, Nucreic Acids Research, 32(17), 5291-5302, 20041001
- Effects of mutation and ligand binding on the compressibility of protein, Progress in Biotechnology, 19, 63-70, 20020401
- Mass spectrometry of hydrogen/deuterium exchange of \textit{Escherichia coli} dihydrofolate reductase: Effects of loop mutations, The Journal of Biochemistry, 135, 487-494, 20040401
- Effects of point mutations at the flexible loop glycine-67 of \textit{Escherichia coli} dihydrofolate reductase on its stability and function, The Journal of Biochemistry, 119, 703-710, 19960401
- Acid and thermal unfolding of \textit{Escherichia coli} dihydrofolate reductase, The Journal of Biochemistry, 120, 946-953, 19960401
- A large compressibility change of protein induced by a single amino acid substitution, Protein Science, 5, 542-545, 19960401
- Nonadditive effects of double mutations at the flexible loops, glycine-67 and glycine-121, of \textit{Escherichia coli} dihydrofolate reductase on its stability and function, The Journal of Biochemistry, 123, 33-41, 19980401
- Effects of point mutations at the flexible loop alanine-145 of \textit{Escherichia coli} dihydrofolate reductase on its stability and function, The Journal of Biochemistry, 123, 839-846, 19980401
- Acetonitrile-protein interactions; amino acid solubility and preferential solvation, Biochimica et Biophysica Acta, 1387, 195-205, 19980401
- Large flexibility of dihydrofolate reductase as revealed by temperature effects on the volume and compressibility, Chemistry Letters, 1999, 507-508, 19990401
- Single amino acid substitutions in flexible loops can induce large compressibility changes in dihydrofolate reductase, The Journal of Biochemistry, 128, 21-27, 20000401
- Effects of five-tryptophan mutations on structure, stability and function of \textit{Escherichia coli} dihydrofolate reductase, The Journal of Biochemistry, 130, 439-447, 20010401
- Pressure effects on enzyme functions, J. Biol. Macromol., 23-29, 20070701
- Discoveries of deep-sea piezophiles, and their pressure adapted enzymes., Proceedings of the 4th International Conference on High Pressure Bioscience and Biotechnology, 1, 114-121, 20071001
- Proteins, under high-pressure environments -Discoveries of deep-sea piezophiles, and their pressure adapted enzymes-., The proceedings of International Symposium on Extremophiles and Their Applications, 148-155, 20071001
- Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: implication of hydrophobic interactions, J. Biochem. (Tokyo), 137, 643-672, 20051001
- Pressure dependence of activity and stability of dihydrofolate reductases of the deep-sea bacterium Moritella profunda and Escherichia coli, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1824(3), 511-519, 201203
- Vacuum-ultraviolet circular dichroism of Escherichia coli dihydrofolate reductase: Insight into the contribution of tryptophan residues, CHEMICAL PHYSICS LETTERS, 572, 111-114, 20130530
- Thermodynamic and functional characteristics of deep-sea enzymes revealed by pressure effects, EXTREMOPHILES, 17(5), 701-709, 201309
- Solvent environments significantly affect the enzymatic function of Escherichia coli dihydrofolate reductase: Comparison of wild-type protein and active-site mutant D27E, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1834(12), 2782-2794, 201312
- Effects of salt on the structure, stability, and function of a halophilic dihydrofolate reductase from a hyperhalophilic archaeon, Haloarcula japonica strain TR-1, EXTREMOPHILES, 19(2), 479-493, 201503
- Vacuum-Ultraviolet Circular Dichroism Spectra of Escherichia coli Dihydrofolate Reductase and Its Mutants: Contributions of Phenylalanine and Tyrosine Side Chains and Exciton Coupling of Two Tryptophan Side Chains, JOURNAL OF PHYSICAL CHEMISTRY B, 119(41), 13002-13008, 20151015
- High pressure NMR reveals active-site hinge motion of folate-bound Escherichia coli dihydrofolate reductase, BIOCHEMISTRY, 39(42), 12789-12795, 2000
- Environmental Adaptation of Dihydrofolate Reductase from Deep-Sea Bacteria., Sub-cellular biochemistry, 72, 2015
- Pressure adaptation of 3-isopropylmalate dehydrogenase from an extremely piezophilic bacterium is attributed to a single amino acid substitution., Extremophiles : life under extreme conditions, 20(2), 2016
- Similar structural stabilities of 3-isopropylmalate dehydrogenases from the obligatory piezophilic bacterium Shewanella benthica strain DB21MT-2 and its atmospheric congener S. oneidensis strain MR-1., Biochimica et biophysica acta, 1866(5-6)
- Halophilic mechanism of the enzymatic function of a moderately halophilic dihydrofolate reductase from Haloarcula japonica strain TR-1., Extremophiles : life under extreme conditions, 21(3), 2017
Publications such as books
- Functional, structural, and thermodynamic characteristics of enzymes from deep-sea microorganisms., Microbial Catalysts Volume 1, Nova Science Publisher, 2019, Scholarly Book, Joint work, E. Ohmae, Y. Miyashita, and C. Kato