Masanobu Wada

Last Updated :2020/07/01

Affiliations, Positions
Graduate School of Humanities and Social Sciences, Professor
E-mail
wadahiroshima-u.ac.jp
Other Contact Details
1-7-1 KAGAMIYAMA, HIGASHIHIROSHIMA, HIROSHIMA, Japan
TEL : (+81)82-424-6584 FAX : (+81)82-424-6584
Self-introduction
I major in muscle physiology and am interested in the mechanisms for muscle fatigue recently.

Basic Information

Major Professional Backgrounds

  • 1996/04/01, Hiroshima University, Faculty of Integrated Arts & Sciences, Associate Professor
  • 2006/04/01, Hiroshima University, Graduate School of Integrated Arts & Sciences, Professor
  • 1988/11/01, 1996/03/31, Hiroshima University, Faculty of Integrated Arts & Sciences, Lecturer
  • 2004/04/01, 2006/03/31, Hiroshima University, Faculty of Integrated Arts & Sciences, Professor

Educational Backgrounds

  • University of Tsukuba, Japan, 1985/04, 1987/03
  • Gifu University, Faculty of Education, Japan, 1978/04, 1982/03

Academic Degrees

  • Doctor of Philosophy, University of Tsukuba
  • Master, University of Tsukuba

Educational Activity

  • 【Bachelor Degree Program】School of Integrated Arts and Sciences : Department of Integrated Arts and Sciences
  • 【Master's Program】Graduate School of Integrated Arts and Sciences : Integrated Arts and Sciences
  • 【Doctoral Program】Graduate School of Integrated Arts and Sciences : Integrated Arts and Sciences

In Charge of Primary Major Programs

  • Integrated Arts and Sciences

Research Fields

  • Complex systems;Health / Sports science;Sports science

Research Keywords

  • muscle fatigue
  • Skeletal muscle
  • excitation-contraction coupling
  • exercise
  • Isoform
  • sarcolasmic reticulum

Affiliated Academic Societies

  • The Japanese Society of Physical Fitness and Sport Medicine
  • Japan Society of Exercise and Sports Physiology, 1996
  • American Physiological Society

Educational Activity

Course in Charge

  1. 2020, Liberal Arts Education Program1, Intensive, Practicum in SportsA
  2. 2020, Liberal Arts Education Program1, 3Term, Sports Theory and Exercise
  3. 2020, Undergraduate Education, 2Term, Experimental methodsIn Sport and Health Sciences B
  4. 2020, Undergraduate Education, 2Term, Laboratory Work in Sport and Health Sciences B
  5. 2020, Undergraduate Education, 3Term, Seminar in Sport and Health Sciences C
  6. 2020, Undergraduate Education, 1Term, Physiology and biochemistry of muscle
  7. 2020, Undergraduate Education, 1Term, Introductory Lectures of Human Movement Sciences I
  8. 2020, Graduate Education (Master's Program) , 3Term, Physiology and Biochemistry of Exercise

Research Activities

Academic Papers

  1. Favourable associations between the myosin heary-chain and light-chain isoforms in human skeletal muscle, Pfl(]E88DB[)gers Archive, 416, 689-693, 19900401
  2. Expression of hybrid isomyos Changes in myosin heavy-chain and light-chain isoforms following sustained exercise, Integration of Medical and Sport Sciences, 309-317, 19920401
  3. Relationship between alkali light-chain complement and myosin heavy-chain isoforms in single fast-twitch fibers of rat and rabbit, European Journal of Biochemistry, 214, 157-161, 19930401
  4. Heredity of muscle fibre composition estimated from a selection experiment in rat, European Journal of Applied Physiology, 66, 85-89, 19930401
  5. Muscle fiber transformation following increased contractile activity, 44, 809-816, 19940401
  6. Expressions of myosin heavy chain IId isform in rat soleus muscle during hindlimb suspension, Acta Physiol. Scand, 143, 131-132, 19910401
  7. Isomyosin patterns of single type IIB, IID and IIA fibres from rabbit skeletal muscle, Journal of Muscle Research and Cell Motility, 16, 237-242, 19950401
  8. Distribution of Myosin Isoforms in Skeletal Muscle : Various Kinds, Functional Significance, and Training-Induced Change, Japanese Journal of Physical Fitness and Sports Medicine, 44(5), 483-502, 19950401
  9. Expression of hybrid isomyosins in human Skeletal muscle, Amenican Journal of Physiology, 271, C1250-C1255, 19960401
  10. Effects of exhastive exercise on sarcoplasmic reticulum ATPase -comparison of short- and long-term exercise, Jpn. J. Phys. Fitress Sports Med., 47(1), 63-72, 19980401
  11. Loss of sarcoplasmic reticulum membrane integrity after eccentric contractions, Acta Physiol. Scand, 161, 581-582, 19970401
  12. Effects of hindlimb suspension on patterns of myosin isoforms in rat soleus muscle, Jpn. J. Phys. Fitress Sports Med., 46(3), 279-288, 19970401
  13. Age effect on expression of myosin heavy and light chain isoforms in suspended rat soleus muscle, J. Appl. Physiol., 86, 1483-1489, 19990401
  14. Sarcoplasmic reticulum function of rat diaphragm following five-day inactivity, Adv. Exerc. Sports Physiol., 7, 27-32, 20010401
  15. Altered sarcoplasmic reticulum function in rat diaphragm after high-intensity exercise, Acta Physiol. Scand, 176, 227-232, 20020401
  16. Effects of endurance training and acute exercise on sarcoplasmic reticulum function rat fast- and slow-twitch skeletal muscles, Eur. J. Appl. Physiol., 89, 142-149, 20030401
  17. Endurance training-induced in alkali light chain patterns in type IIB fibers of the rat, J. Appl. Physiol., 94(923-929), 20030401
  18. Oxidation of sarcoplasmic reticulum Ca2+-ATPase induced by high-intensity exercise, 446, 394-399, 20030401
  19. Different time course of changes in sarcoplasmic reticulum and myosin isoforms in rat soleus muscle at early stage of hyperthyroidism, Acta Physiol. Scand, 2004, 79-87, 20040401
  20. N-acetylcysteine fails to modulate the in vitro function of sarcoplasmic reticulum of diaphragm in final phase of fatigue, Acta Physiol. Scand, 184, 195-202, 20050401
  21. Effects of reduced glycogen on structure and in vitro function of rat sarcoplasmic reticulum, 452, 117-123, 20060401
  22. Myofibrillar protein oxidation and contractile dysfunction in hyperthyroid rat diaphragm, Journal of Applied Physiology, 102, 1850-1855, 20070201
  23. Effects of high-intensity training and acute exercise on in vitro function of rat sarcoplasmic reticulum, European Journal of Applied Phsiplogy, 99, 641-649, 20070101
  24. Time course of changes in in vitro sarcoplasmic reticulum Ca2+-handling and Na+-K+-ATPase activity during repetitive contractions, Pflügers Arch, 456, 601-609, 20080601
  25. No relationship between enzyme activity and structure of nucleotide binding site in sarcoplasmic reticulum Ca2+-ATPase from short-term stimulated rat muscle, Acta Physiol, 196, 401-409, 20090201
  26. Effect of dithiothreitol on Ca2+-ATPase activity of sarcoplasmic reticulum in rat skeletal muscle after high-intensity exercise, JAPANESE JOURNAL OF PHYSICAL FITNESS AND SPORTS MEDICINE, 50(3), 325-331, 200106
  27. Altered sarcoplasmic reticulum function in rat diaphragm after high-intensity exercise, ACTA PHYSIOLOGICA SCANDINAVICA, 176(3), 227-232, 200211
  28. Endurance training-induced changes in alkali light chain patterns in type IIB fibers of the rat, JOURNAL OF APPLIED PHYSIOLOGY, 94(3), 923-929, 200303
  29. Effect of endurance training and acute exercise on sarcoplasmic reticulum function in rat fast- and slow-twitch skeletal muscles, EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, 89(2), 142-149, 200304
  30. Oxidation of sarcoplasmic reticulum Ca2+-ATPase induced by high-intensity exercise, PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 446(3), 394-399, 200306
  31. Different time course of changes in sarcoplasmic reticulum and myosin isoforms in rat soleus muscle at early stage of hyperthyroidism, ACTA PHYSIOLOGICA SCANDINAVICA, 180(1), 79-87, 200401
  32. Effects of thyroid hormone on sarcoplasmic reticulum Ca2+ uptake and contractile properties in rat soleus muscle, JAPANESE JOURNAL OF PHYSICAL FITNESS AND SPORTS MEDICINE, 53(5), 509-517, 200410
  33. N-acetylcysteine fails to modulate the in vitro function of sarcoplasmic reticulum of diaphragm in the final phase of fatigue, ACTA PHYSIOLOGICA SCANDINAVICA, 184(3), 195-202, 200507
  34. Oxidation of myosin heavy chain and reduction in force production in hyperthyroid rat soleus, JOURNAL OF APPLIED PHYSIOLOGY, 100(5), 1520-1526, 200605
  35. Effects of reduced glycogen on structure and in vitro function of rat sarcoplasmic reticulum Ca2+-ATPase, PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 452(1), 117-123, 200604
  36. Changes in sarcoplasmic reticulum Ca2+-sequestering capacity during recovery following high-intensity exercise: comparisons between fast- and slow-twitch muscles, JAPANESE JOURNAL OF PHYSICAL FITNESS AND SPORTS MEDICINE, 55(5), 503-511, 200610
  37. Effects of high-intensity training and acute exercise on in vitro function of rat sarcoplasmic reticulum, EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, 99(6), 641-649, 200704
  38. Myofibrillar protein oxidation and contractile dysfunction in hyperthyroid rat diaphragm, JOURNAL OF APPLIED PHYSIOLOGY, 102(5), 1850-1855, MAY 2007
  39. Relationship between oxidation of myofibrillar proteins and contractile properties in soleus muscles from hyperthyroid rat, JAPANESE JOURNAL OF PHYSICAL FITNESS AND SPORTS MEDICINE, 56(5), 473-480, 200710
  40. Alterations in in vitro function and protein oxidation of rat sarcoplasmic reticulum Ca2+-ATPase during recovery from high-intensity exercise, EXPERIMENTAL PHYSIOLOGY, 93(3), 426-433, 20080301
  41. Time course of changes in in vitro sarcoplasmic reticulum Ca(2+)-handling and Na(+)-K(+)-ATPase activity during repetitive contractions, PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 456(3), 601-609, 200806
  42. Effect of high-intensity training and acute exercise on Ca(2+)-sequestering function of sarcoplasmic reticulum: Role of oxidative modification, JAPANESE JOURNAL OF PHYSICAL FITNESS AND SPORTS MEDICINE, 57(3), 327-338, 200806
  43. Chicken breast attenuates high-intensity-exercise-induced decrease in rat sarcoplasmic reticulum Ca2+ handling, INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM, 18(4), 399-411, 200808
  44. No relationship between enzyme activity and structure of nucleotide binding site in sarcoplasmic reticulum Ca2+-ATPase from short-term stimulated rat muscle, ACTA PHYSIOLOGICA, 196(4), 401-409, 200908
  45. A Novel Glycerophosphodiester Phosphodiesterase, GDE5, Controls Skeletal Muscle Development via a Non-enzymatic Mechanism, JOURNAL OF BIOLOGICAL CHEMISTRY, 285(36), 27652-27663, 20100903
  46. EFFECTS OF ECCENTRIC CONTRACTIONS ON IN VITRO Na+-K+-ATPase ACTIVITY AND SARCOPLASMIC RETICULUM Ca2+-SEQUESTERING IN RAT SKELETAL MUSCLE, JAPANESE JOURNAL OF PHYSICAL FITNESS AND SPORTS MEDICINE, 59(4), 337-348, 201008
  47. Increased fatigue resistance linked to Ca2+-stimulated mitochondrial biogenesis in muscle fibres of cold-acclimated mice, JOURNAL OF PHYSIOLOGY-LONDON, 588(21), 4275-4288, 20101101
  48. The effects of eccentric contraction on myofibrillar proteins in rat skeletal muscle, EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, 110(5), 943-952, 201011
  49. Three calpain isoforms are autolyzed in rat fast-twitch muscle after eccentric contractions, JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, 35(2), 179-189, 201404
  50. Contribution of impaired myofibril and ryanodine receptor function to prolonged low-frequency force depression after in situ stimulation in rat skeletal muscle, JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, 36(3), 275-286, 201506
  51. Muscle dysfunction associated with adjuvant-induced arthritis is prevented by antioxidant treatment, SKELETAL MUSCLE, 5, 20150709
  52. Calcium kinetics of sarcoplasmic reticulum and muscle fatigue, J. Phys. Fitness Sports Med., 2, 169-178, 2013
  53. Enhanced activity of eccentric contraction induces alterations in in vitro sarcoplasmic reticulum Ca2+ handling in rat hindlimb muscles, J. Phys. Fitness Sports Med., 4, 117-127, 2015
  54. 日本運動生理学雑誌, 23, 29-36, 2016
  55. Predominant cause of prolonged low-frequency force depression changes during recovery after in situ fatiguing stimulation of rat fast-twitch muscle, AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 311(5), R919-R929, 201611
  56. Role of calpain in eccentric contraction-induced proteolysis of Ca2+ regulatory proteins and force depression in rat fast-twitch skeletal muscle, JOURNAL OF APPLIED PHYSIOLOGY, 122(2), 396-405, 201702
  57. Neuromuscular electrical stimulation prevents skeletal muscle dysfunction in adjuvant-induced arthritis rat, PLOS ONE, 12(6), 20170621
  58. Time Course Analysis of Skeletal Muscle Pathology of GDE5 Transgenic Mouse, PLOS ONE, 11(9), 20160922
  59. L-arginine ingestion inhibits eccentric contraction-induced proteolysis and force deficit via S-nitrosylation of calpain, PHYSIOLOGICAL REPORTS, 6(2), 201801
  60. The efficacy of microcurrent therapy on eccentric contraction-induced muscle damage in rat fast-twitch skeletal muscle, Open J. Appl. Sci., 8, 89-102, 2018
  61. Ingestion of soy protein isolate attenuates eccentric contraction-induced force depression and muscle proteolysis via inhibition of calpain-1 activation in rat fast-twitch skeletal muscle, Nutrition, 58, 23-29, 2018
  62. Nutritional aspects of a year-long wheelchair dance intervention in bedridden individuals with severe athetospastic cerebral palsy rated to GMFCS level V, Gazzetta Medica Italiana Archivio per le Scienze Mediche, 177, 360-366, 2018
  63. Neuromuscular electrical stimulation prevents preferential skeletal muscle myosin loss in steroid-denervation rats, Frontiers in Physiology, in press, 2018
  64. Preconditioning contractions prevent the delayed onset of myofibrillar dysfunction after damaging eccentric contractions, J. Physiol., 596, 4427-4442, 2018
  65. Thermal pretreatment facilitates recovery from prolonged low-frequency force depression in rat fast-twitch muscle, Physiol Rep, 6(17), 2018
  66. Endurance training-based tapering fails to improve fatigue resistance of rat skeletal muscle, Adv. Exerc. Sports Physiol., 21, 37-45, 2015
  67. Myosin heavy chain expression and oxidative modifications in diabetic rat hearts, Open J. Appl. Sci., 2, 248-256, 2012
  68. Treatment with EUK-134 improves sarcoplasmic reticulum Ca2+ release but not myofibrillar Ca2+ sensitivity after fatiguing contraction of rat fast-twitch muscle, AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 316(5), R543-R551, 201905

Invited Lecture, Oral Presentation, Poster Presentation

  1. Regulation of contractile properties in skeletal muscle fibers (Symposium: Why does skeletal muscle have fast and slow muscle fibers?, Masanobu Wada, The 90th Annual Meeting of the Physiological Society of Japan, 2013/09, With Invitation
  2. Decline of S-glutathinylation of troponin I fast isoform induces prolonged low-frequency force depression in the late stage of recovery, Daiki Watanabe, Masanobu Wada, 44nd European Muscle Conference, 2015/09, Without Invitation
  3. Effects of increased muscle glycogen on SERCA function in rat fast-twitch muscle, Mishima, T., Watanabe, D. and Wada, M., 44nd European Muscle Conference, 2015/09, Without Invitation, Warsaw
  4. Effects of dietary Kaempferia parviflora on contractile force in rat skeletal muscle, Watanabe, D. and Wada, M., 8th Federation of the Asian and Oceanian Physiological Societies Congress, 2015/11, Without Invitation
  5. Predominant cause of prolonged low-frequency force depression during recovery from in situ fatiguing stimulation, Watabane, D., Wada, M., 45th European Muscle Conference, 2016, Without Invitation, Montpellier
  6. Effects of glycogen-loading on recovery process after muscle fatigue, Mishima, T., Watanabe, D., Wada, M., 45th European Muscle Conference, 2016, Without Invitation, Montpellier
  7. Regulation of contractile properties in skeletal muscle fibers (Symposium: Why does skeletal muscle have fast and slow muscle fibers?, Wada, M., The 90th Annual Meeting of the Physiological Society of Japan, 2013, With Invitation, the Physiological Society of Japan
  8. Mechanisms underlying increases in Ca2+ leakage from sarcoplasmic reticulum with prolonged low-frequency force depression in rat skeletal muscle., Watanabe, D. and Wada, M., Europhysiology 2018, 2018/09, Without Invitation, European Physiological Society, London
  9. L-arginine ingestion inhibits eccentric contraction-induced proteolysis and force deficit via S-nitrosylation of calpain, Kanzaki, K., Watanabe, D., Aibara, C., Kawakami, Y., Yamada, T., Takahashi, Y. and Wada, M., Europhysiology 2018, 2018/09, Without Invitation, London

External Funds

Acceptance Results of Competitive Funds

  1. KAKENHI, Cellular functions of a cytosolic glycerophosphocholine phosphodiesterase GDE5, 2011, 2013
  2. KAKENHI, Clarify the mechanism of attenuating effect at muscle fatigue by carnosine ingestion., 2010, 2011
  3. KAKENHI, Effect of training on paralyzed muscles of persons with spinal cord injury, 2009, 2011
  4. KAKENHI, Does the sarcoplasmic reticulum function contribute to the decline of muscle contractile force following eccentric contraction ?, 2009, 2012
  5. KAKENHI, Physiological functions of novel mammalian glycerophosphodiester phosphodiesterases, 2008, 2010
  6. KAKENHI, Effects of physical therapy on mechanical allodynia and functional changes of muscles in a rat model of neuropathic pain, 2005, 2006
  7. KAKENHI, Mechanisms of Retarded Recovery of Muscular Fatigue After Vigorous Contraction, 2005, 2007
  8. KAKENHI, Can high-intensity training protect against exercise-induced oxidation of sarcoplasmic reticulum Ca^<2+>-ATPase?, 2004, 2006
  9. KAKENHI, The effect of antioxidants on sarcoplasmic reticulum function during muscle fatigue, 2001, 2003
  10. KAKENHI, Effects of endurance training and acute exercise on sarcoplasmic reticulum function, 1999, 2000
  11. KAKENHI, PHYSIOLOGICAL STUDY ABOUT CONDITIONING AND PERFORMANCE IN ATHLETES, 1994, 1996
  12. KAKENHI, 1994, 1994
  13. KAKENHI, 1993, 1993
  14. KAKENHI, 2015, 2017
  15. KAKENHI, 2016, 2017
  16. KAKENHI, 2015