Naoki Isobe

Last Updated :2020/10/16

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Professor
Web Site
E-mail
nisohiroshima-u.ac.jp
Other Contact Details
1-4-4 HIGASHI-HIROSHIMA, Japan
TEL : (+81)82-424-7993 FAX : (+81)82-424-7993
Self-introduction
My research is for prevention of mastitis in dairy cow and goats.

Basic Information

Educational Backgrounds

  • Hiroshima University, Graduate School, Division of Biophere Sciences, Japan, 1993/04, 1996/03
  • Hiroshima University, Faculty of Applied Biological Science, Japan, 1987/04, 1991/03

Academic Degrees

  • Doctor of Agriculture, Hiroshima University
  • Master of Agriculture, Hiroshima University

Educational Activity

  • 【Bachelor Degree Program】School of Applied Biological Science : Department of Applied Biological Science
  • 【Master's Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Bioresource Science
  • 【Doctoral Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Bioresource Science

In Charge of Primary Major Programs

  • Applied Animal and Plant Science Program

Research Fields

  • Agricultural sciences;Animal life science;Animal production science

Research Keywords

  • mammary gland
  • EIA
  • Somatic cell count
  • immunohistochemistry
  • hormone
  • milk
  • feces
  • Mastitis
  • Innate immunity
  • Antimicrobial peptide

Affiliated Academic Societies

  • Japanese Society of Veterinary Science, 2016
  • The American Dairy Science Association, 2009
  • Japanese Association of Animal Science, 1990
  • Japanese Association of Animal Reproduction, 1993
  • Japanese Association of Mastitis, 2006
  • Kansai Association of Animal Science, 1990
  • Chushikoku Mastitis Council, 2013

Educational Activity

Course in Charge

  1. 2020, Liberal Arts Education Program1, 4Term, Biological production and natural environment
  2. 2020, Liberal Arts Education Program1, 1Term, Introductory Seminar for First-Year Students
  3. 2020, Undergraduate Education, 2Term, Reading of Foreign Literature
  4. 2020, Undergraduate Education, 4Term, Introduction to Applied Biological Science II
  5. 2020, Undergraduate Education, 1Term, Introduction to Physiology
  6. 2020, Undergraduate Education, 3Term, Animal Functional Anatomy
  7. 2020, Undergraduate Education, Intensive, Laboratory Work in Animal Functional Anatomy
  8. 2020, Undergraduate Education, Academic Year, Graduation Thesis
  9. 2020, Undergraduate Education, 1Term, Animal Physiology and Production
  10. 2020, Undergraduate Education, Intensive, Laboratory and Field Works in Animal Production I
  11. 2020, Undergraduate Education, 3Term, Laboratory Animal Science
  12. 2020, Undergraduate Education, Intensive, (AIMS) Introductory Physiology of Domestic Animals
  13. 2020, Undergraduate Education, 3Term, Introductory Physiology of Domestic Animals
  14. 2020, Graduate Education (Master's Program) , 4Term, Domestic Animal Endocrinology
  15. 2020, Graduate Education (Master's Program) , 4Term, Animal Histochemistry
  16. 2020, Graduate Education (Doctoral Program) , First Semester, Exercises in Animal Science(B)
  17. 2020, Graduate Education (Doctoral Program) , Second Semester, Exercises in Animal Science(B)
  18. 2020, Graduate Education (Master's Program) , 1Term, Exercises in Bioresource Science A
  19. 2020, Graduate Education (Master's Program) , 2Term, Exercises in Bioresource Science A
  20. 2020, Graduate Education (Master's Program) , 3Term, Exercises in Bioresource ScienceB
  21. 2020, Graduate Education (Master's Program) , 4Term, Exercises in Bioresource ScienceB
  22. 2020, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Bioresource Science
  23. 2020, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Bioresource Science
  24. 2020, Graduate Education (Master's Program) , 2Term, Animal Production Science I
  25. 2020, Graduate Education (Master's Program) , 4Term, Animal Production Science II

Research Activities

Academic Papers

  1. Concentration patterns of antibacterial factors and immunoglobulin A antibody in foremilk fractions of healthy cows., Animal Science Journal, 91(1), e13372., 2020
  2. Effect of oral administration of colostrum whey to peripartum goat on antimicrobial peptides in postpartum milk., Animal Science Journal, 91(1), e13365, 2020
  3. Seasonal variations in the concentration of antimicrobial components in milk of dairy cows., Animal Science Journal, 91, e13427, 2020
  4. Effects of intrauterine infusion of bacterial lipopolysaccharides on the mammary gland inflammatory response in goats, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 219, 202001
  5. Effects of Oral Administration of Lactobacillus reuteri on Mucosal Barrier Function in the Digestive Tract of Broiler Chicks, JOURNAL OF POULTRY SCIENCE, 57(1), 67-76, 202001
  6. Short communication: Production of antimicrobial peptide S100A8 in the goat mammary gland and effect of intramammary infusion of lipopolysaccharide on S100A8 concentration in milk, JOURNAL OF DAIRY SCIENCE, 102(5), 4674-4681, 201905
  7. Age-related modulation of the isthmic and uterine mucosal innate immune defense system in laying hens, POULTRY SCIENCE, 98(7), 3022-3028, 201907
  8. Effects of the routine multiple vaccinations on the expression of innate immune molecules and induction of histone modification in ovarian cells of layer chicks, POULTRY SCIENCE, 98(10), 5127-5136, 201910
  9. Blood Testosterone Concentration and Testosterone-induced Aggressive Behavior in Male Layer Chicks: Comparison between Isolated- and Grouped-Raising, JOURNAL OF POULTRY SCIENCE, 56(4), 290-297, 201910
  10. Effects of Salmonella enteritidis Vaccination on the Expression of Innate Immune Molecules and Histone Modifications in the Follicular Theca of Laying Hens, JOURNAL OF POULTRY SCIENCE, 56(4), 298-307, 201910
  11. Association between bovine leukemia virus proviral load and severity of clinical mastitis, JOURNAL OF VETERINARY MEDICAL SCIENCE, 81(10), 1431-1437, 201910
  12. Detection of APAF1 mutation in Holstein cows and mummified foetuses in Japanese dairy herds, REPRODUCTION IN DOMESTIC ANIMALS, 53(1), 137-142, 201802
  13. Microcystin-leucine-arginine Modulates the Expression Patterns of Proinflammatory Cytokines and an Apoptotic Gene in Chicken Liver, JOURNAL OF POULTRY SCIENCE, 55(1), 70-77, 201801
  14. Blood ionized calcium levels and acute-phase blood glucose kinetics in goats after intramammary infusion of lipopolysaccharide, JOURNAL OF VETERINARY MEDICAL SCIENCE, 80(2), 242-246, 201802
  15. Innate antiviral immune response against infectious bronchitis virus and involvement of prostaglandin E2 in the uterine mucosa of laying hens, THERIOGENOLOGY, 110, 122-129, 20180401
  16. Effects of Interleukin-1 beta and-6 on the Expression of Ion Transporters Involved in Eggshell Mineralization in Cultured Hen Uterine Mucosal Tissue, JOURNAL OF POULTRY SCIENCE, 55(2), 142-149, 201804
  17. Changes in plasma concentrations of S100A7 and S100A8 in dairy cows during pregnancy, REPRODUCTION IN DOMESTIC ANIMALS, 53(4), 1013-1015, 201808
  18. Effects of colostrum whey on immune function in the digestive tract of goats, ANIMAL SCIENCE JOURNAL, 89(8), 1152-1160, 201808
  19. Investigation of the binding of goat cathelicidin-7 to lipopolysaccharide and leucocidal suppression of pro-inflammatory cytokines., Small Ruminant Research, 168:, 101-106, 2018
  20. Changes in the concentrations of somatic cell counts, lingual antimicrobial peptide and lactoperoxidase activity in milk at periovulatory period in dairy cows, ANIMAL SCIENCE JOURNAL, 88(3), 484-488, 2017
  21. Effects of inhibitors of transcription factors, nuclear factor-kappa B and activator protein 1, on the expression of proinflammatory cytokines and chemokines induced by stimulation with Toll-like receptor ligands in hen vaginal cells, POULTRY SCIENCE, 96(3), 723-730, 2017
  22. Control mechanisms for producing antimicrobial factors in ruminant mammary gland, ANIMAL SCIENCE JOURNAL, 88(7), 937-943, 2017
  23. Effect of steroid hormones on the innate immune response induced by Staphylococcus aureus in the goat mammary gland, REPRODUCTION IN DOMESTIC ANIMALS, 52(4), 579-584, 2017
  24. Changes in the concentrations of somatic cell counts, lingual antimicrobial peptide and lactoperoxidase activity in milk at periovulatory period in dairy cows., Animal Science Journal, 88(3), 484-488, 20170301
  25. Effect of nutrient levels during the far-off period on postpartum productivity in dairy cows., Animal Science Journal, 2017
  26. The extension of raw milk quality during storage through supplementation of hydrocyanic acid from fresh cassava peel in dairy cattle diet. Songklanakarin Journal of Science and Technology (in press), Songklanakarin Journal of Science and Technology, 2017
  27. The inhibitory effect of sodium thiocyanate and sodium percarbonate rations on microorganism growth in raw milk samples as an effective treatment to extend milk quality during storage., Songklanakarin Journal of Science and Technology, 39(1), 2017
  28. Effects of dietary levels of fresh cassava pulp in dairy cattle diet on productive performance and keeping quality of raw milk. Songklanakarin Journal of Science and Technology (in press), Songklanakarin Journal of Science and Technology, 2017
  29. Microcystin-LR modulates the expression patterns of proinflammatory cytokines and apoptotic gene in the liver of chicken., Journal of Poultry Science, 2017
  30. Cellular and soluble components decrease the viable pathogen counts in milk from dairy cows with subclinical mastitis., The Journal of Veterinary Medical Science, 79(8), 1389-1393, 201708
  31. Effects of Probiotics on the Expression of Cathelicidins in Response to Stimulation by Salmonella Minnesota Lipopolysaccharides in the Proventriculus and Cecum of Broiler Chicks, JOURNAL OF POULTRY SCIENCE, 53(4), 298-304, 2016
  32. Change in viable bacterial count during preservation of milk derived from dairy cows with subclinical mastitis and its relationship with antimicrobial components in milk., The Journal of Veterinary Medical Science, 78(8), 1245-1250, 201608
  33. Expression of lipases and lipid receptors in sperm storage tubules and possible role of fatty acids in sperm survival in the hen oviduct., Theriogenology, 85, 1334-1342, 20160415
  34. Effects of viral-associated molecular patterns on the expression of cathelicidins in the hen vagina., Journal of Poultry Science, 2016
  35. The inhibitory effect of sodium thiocyanate and sodium percarbonate rations on microorganism growth in raw milk samples as an effective treatment to extend milk quality during storage., Songklanakarin Journal of Science and Technology, 39(1), 2016
  36. Effects of inhibitors of transcription factors, nuclear factor-κB and activator protein 1, on the expression of proinflammatory cytokines and chemokines induced by stimulation with Toll-like receptor ligands in hen vaginal cells, Poultry Science, 2016
  37. The effect of estrogen on the early cytotoxic response to IB virus infection in hen oviduct, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 164(42737), 56-66, 20150315
  38. Dynamics of lingual antimicrobial peptide, lactoferrin concentrations and lactoperoxidase activity in the milk of cows treated for clinical mastitis, ANIMAL SCIENCE JOURNAL, 86(2), 153-158, 201502
  39. Effects of Probiotics on the Expression and Localization of Avian beta-defensins in the Proventriculus of Broiler Chicks, JOURNAL OF POULTRY SCIENCE, 52(1), 57-67, 201501
  40. Expression of cathelicidins mRNA in the goat mammary gland and effect of the intramammary infusion of lipopolysaccharide on milk cathelicidin-2 concentration, VETERINARY MICROBIOLOGY, 170(42737), 125-134, 20140514
  41. Effects of different TLR ligands on the expression of proinflammatory cytokines and avian beta-defensins in the uterine and vaginal tissues of laying hens, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 162(42798), 132-141, 20141215
  42. Innate immune functions in hen reproductive organs, AVIAN BIOLOGY RESEARCH, 7(1), 39-47, 2014
  43. Effects of calcium salts of medium-chain fatty acids on plasma metabolite and hormone concentrations in early lactating dairy cows, ANIMAL PRODUCTION SCIENCE, 54(10), 1699-1702, 2014
  44. Messenger RNA expression and immunolocalization of psoriasin in the goat mammary gland and its milk concentration after an intramammary infusion of lipopolysaccharide, VETERINARY JOURNAL, 202(1), 89-93, 2014
  45. Effects of avian infectious bronchitis virus antigen on eggshell formation and immunoreaction in hen oviduct, THERIOGENOLOGY, 81(8), 1129-1138, 2014
  46. Toll-like receptor signaling for the induction of mucin expression by lipopolysaccharide in the hen vagina, POULTRY SCIENCE, 93(3), 673-679, 2014
  47. Relationship between the somatic cell count in milk and reproductive function in peripartum dairy cows, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 60(6), 433-437, 2014
  48. Relationship between concentration of lingual antimicrobial peptide and somatic cell count in milk of dairy cows, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 153(42798), 298-301, 20130615
  49. Expression of tight junction molecule claudins" in the lower oviductal segments and their changes with egg-laying phase and gonadal steroid stimulation in hens", THERIOGENOLOGY, 79(2), 211-218, 20130115
  50. Comparison of cadherin and integrin localization in bovine cystic and healthy follicles, ANIMAL SCIENCE JOURNAL, 84(4), 303-309, 2013
  51. Dectin-1 gene polymorphism is associated with susceptibility to nonspecific digestive disorders and cytokine expression in rabbits, JOURNAL OF ANIMAL SCIENCE, 91(9), 4051-4059, 2013
  52. Lingual antimicrobial peptide and lactoferrin concentrations and lactoperoxidase activity in bovine colostrum are associated with subsequent somatic cell count, ANIMAL SCIENCE JOURNAL, 84(11), 751-756, 2013
  53. Induction of avian beta-defensins by CpG oligodeoxynucleotides and proinflammatory cytokines in hen vaginal cells in vitro, REPRODUCTION, 145(6), 621-631, 2013
  54. Effects of Probiotics on the Localization of T Cell Subsets in the Intestine of Broiler Chicks, JOURNAL OF POULTRY SCIENCE, 50(3), 275-281, 2013
  55. Effects of Lipopolysaccharide on the Recruitment of T Cells in the Seminal Tract of Roosters, JOURNAL OF POULTRY SCIENCE, 50(1), 68-73, 2013
  56. Effects of Repeated Lipopolysaccharide Stimulation on the Development of Antigen-presenting Cells and T Cells Pool in Hen Vagina, JOURNAL OF POULTRY SCIENCE, 50(1), 83-89, 2013
  57. Induction of mucin expression by estrogen and lipopolysaccharide in the lower oviductal segments in hens, POULTRY SCIENCE, 92(12), 3205-3213, 2013
  58. Relationship between concentration of lingual antimicrobial peptide and somatic cell count in milk of dairy cows, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 153(3-4), 298-301, 2013
  59. Effects of Probiotics on the Localization of T Cell Subsets in the Intestine of Broiler Chicks, JOURNAL OF POULTRY SCIENCE, 50(3), 275-281, 2013
  60. Expression of Toll-like receptors and effects of lipopolysaccharide on the expression of proinflammatory cytokines and chemokine in the testis and epididymis of roosters, POULTRY SCIENCE, 91(8), 1997-2003, 2012
  61. Differences in the mucosal surface barrier formed by mucin in the lower oviductal segments between laying and molting hens, POULTRY SCIENCE, 91(5), 1173-1178, 20120501
  62. Differential immunolocalization between lingual antimicrobial peptide and lactoferrin in mammary gland of dairy cows, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 145(42737), 499-504, 20120115
  63. Effect of enterotoxigenic Escherichia coli vaccine on innate immune function of bovine mammary gland infused with lipopolysaccharide, JOURNAL OF DAIRY SCIENCE, 95(9), 5067-5074, 2012
  64. Effects of lipopolysaccharide and interleukins on the expression of avian beta-defensins in hen ovarian follicular tissue, POULTRY SCIENCE, 91(11), 2877-2884, 2012
  65. Effects of lipopolysaccharide on the expression of proinflammatory cytokines and chemokines and influx of leukocytes in the hen ovary, POULTRY SCIENCE, 90(9), 2054-2062, 20110901
  66. Effects of lipopolysaccharide on the expression of proinflammatory cytokines and chemokines and the subsequent recruitment of immunocompetent cells in the oviduct of laying and molting hens, POULTRY SCIENCE, 90(10), 2332-2341, 20111001
  67. Differential localization of lingual antimicrobial peptide in the digestive tract mucosal epithelium of calves, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 142(42737), 87-94, 20110715
  68. Expression of Toll-like receptors and avian beta-defensins and their changes in response to bacterial components in chicken sperm, POULTRY SCIENCE, 90(2), 417-425, 20110201
  69. Detection of Avian Beta-defensins mRNA and Proteins in Male Reproductive Organs in Chicken, JOURNAL OF POULTRY SCIENCE, 48(4), 275-280, 2011
  70. Changes in the Density of Immunoreactive Avian beta-Defensin-3 and-11 in the Hen Uterus in Response to Lipopolysaccharide Inoculation, JOURNAL OF POULTRY SCIENCE, 48(1), 73-77, 2011
  71. Lactoperoxidase activity in milk is correlated with somatic cell count in dairy cows, JOURNAL OF DAIRY SCIENCE, 94(8), 3868-3874, 2011
  72. Follicular Persistence Induced by Adrenocorticotropic Hormone Administration in Goats, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 57(2), 212-216, 2011
  73. Analysis of Changes in the Expression of Transforming Growth Factor-beta s in the Utero-vaginal Junction of Hen Oviduct in Response to Sperm Concerning Their Significance in Sperm Survivability, JOURNAL OF POULTRY SCIENCE, 47(4), 326-332, 2010
  74. Effects of Feeding Probiotics on the Localization of Cells Containing Immunoreactive Interleukin-6 in the Intestine of Broiler Chicks, JOURNAL OF POULTRY SCIENCE, 47(3), 250-255, 2010
  75. Changes in the Localization of Immunoreactive Avian Beta-Defensin-8,-10 and-12 in Hen Ovarian Follicles during Follicular Growth, JOURNAL OF POULTRY SCIENCE, 47(1), 77-84, 2010
  76. Expression of Toll-like receptors (TLRs) and TLR4 response to lipopolysaccharide in hen oviduct, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 127(42798), 259-268, 20090215
  77. Co-transfer of parthenogenotes and single porcine embryos leads to full-term development of the embryos, ANIMAL REPRODUCTION SCIENCE, 112(42737), 8-21, 2009
  78. Changes in the expression of interleukin-1 beta and lipopolysaccharide-induced TNF factor in the oviduct of laying hens in response to artificial insemination, REPRODUCTION, 137(3), 527-536, 2009
  79. Existence of functional lingual antimicrobial peptide in bovine milk, JOURNAL OF DAIRY SCIENCE, 92(6), 2691-2695, 2009
  80. Identification of Ghrelin in Fertilized Eggs of Chicken, JOURNAL OF POULTRY SCIENCE, 46(3), 257-259, 2009
  81. Intramammary challenge of lipopolysaccharide stimulates secretion of lingual antimicrobial peptide into milk of dairy cows, JOURNAL OF DAIRY SCIENCE, 92(12), 6046-6051, 2009
  82. Immunolocalization of avian beta-defensins in the hen oviduct and their changes in the uterus during eggshell formation, REPRODUCTION, 138(6), 971-978, 2009
  83. ★, Existence of Functional Lingual Antimicrobial Peptide (LAP) in Bovine Milk, Journal of Dairy Science, 92(6), 2691-2695, 20090601
  84. Immunolocalization of lingual antimicrobial peptide (LAP) in the bovine mammary gland, ANIMAL SCIENCE JOURNAL, 80(4), 446-450, 2009
  85. Immunolocalization of lingual antimicrobial peptide (LAP) in the bovine mammary gland, ANIMAL SCIENCE JOURNAL, 80(4), 446-450, 2009
  86. Expression of avian beta-defensins in the oviduct and effects of lipopolysaccharide on their expression in the vagina of hens, POULTRY SCIENCE, 87(5), 979-984, 2008
  87. Poultry production profile and expected future projection in Bangladesh, WORLDS POULTRY SCIENCE JOURNAL, 64(1), 99-117, 2008
  88. Expression of vascular endothelial growth factor receptors in bovine cystic follicles, REPRODUCTION IN DOMESTIC ANIMALS, 43(3), 267-271, 2008
  89. Changes in the localization of immunoreactive avian beta-defensin-12 in ovarian follicles during follicular growth and in response to lipopolysaccharide, JOURNAL OF POULTRY SCIENCE, 45(3), 210-214, 2008
  90. Mechanism of Prolonged Sperm Storage and Sperm Survivability in Hen Oviduct: A Review, AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 60(6), 477-481, 2008
  91. Expression of Avian beta-Defensin 3, an Antimicrobial Peptide, by Sperm in the Male Reproductive Organs and Oviduct in Chickens: An Immunohistochemical Study, POULTRY SCIENCE, 87(12), 2653-2659, 2008
  92. Ovarian Cyclicity and Reproductive Performance of Holstein Cows Carrying the Mutation of Complex Vertebral Malformation in Japan., Reproduction in Domestic Animals, IN PRESS, 20080101
  93. Changes in fecal progestagen profile after excretion in miniature pigs, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 53(5), 1107-1112, 2007
  94. Deficient proliferation and apoptosis in the granulosa and theca interna cells of the bovine cystic follicle, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 53(5), 1119-1124, 2007
  95. Changes in the expression of gallinacins, antimicrobial peptides, in ovarian follicles during follicular growth and in response to lipopolysaccharide in laying hens (Gallus domesticus), REPRODUCTION, 133(1), 127-133, 2007
  96. Follicular cysts in dairy cows, ANIMAL SCIENCE JOURNAL, 78(1), 1-6, 2007
  97. Fecal progestagen and estrone during pregnancy in a giraffe: A case report, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 53(1), 159-164, 2007
  98. Changes in the expression of Toll-like receptor mRNAs during follicular growth and in response to lipopolysaccharide in the ovarian follicles of laying hens, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 53(6), 1227-1235, 2007
  99. Involvement of plasma progesterone, Oestradiol-17 beta and cortisol in ovulatory response to gonadotropin-releasing hormone in dairy cows with cystic follicles, REPRODUCTION IN DOMESTIC ANIMALS, 42(4), 370-375, 2007
  100. Preparation and Application for Immunocytochemistry of Antibody to Gallinacin-3, an Antimicrobial Peptide, in Chicken., Journal of Poultry Science, 44(4), 433-438, 20071001
  101. Changes in the mRNA expressions of sperm Transforming Growth Factor beta-isoforms and their receptors with reference to fertility during in vitro storage of fowl semen., Journal of Poultry Science, 44(2), 189-197, 20070401
  102. Studies of the role of steroid hormone in the regulation of oocyte maturation in cattle, REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 4, 20060203
  103. Expression of transforming growth factor-beta isoforms and their receptors in utero-vaginal junction of hen oviduct in presence or absence of resident sperm with reference to sperm storage, REPRODUCTION, 132(5), 781-790, 2006
  104. Effects of age and gonadal steroids on the localization of antigen presenting cells in the epididymis of the male chicken, gallus domesticus, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 52(3), 363-371, 2006
  105. Effects of age, egg-laying activity, and Salmonella-inoculation on the expressions of gallinacin mRNA in the vagina of the hen oviduct, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 52(2), 211-218, 2006
  106. Enzyme Histochemical Localization of Peroxidase Activity in the Oviduct of Immature, Laying and Non-laying Hens. (),, Journal of Poultry Science, 43(4), 401-407, 20061001
  107. Measurement of fecal progesterone concentration in miniature pig using the direct EIA method, 3, 27-29, 20060301
  108. Studies on substantially increased proteins in follicular fluid of bovine ovarian follicular cysts using 2-D PAGE and MALDI-TOF MS, REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 3, 20050608
  109. Expression of gap junction protein connexin 43 during follicular atresia in the ovary of swamp buffaloes, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 51(5), 675-681, 2005
  110. Microvascular distribution and vascular endothelial growth factor expression in bovine cystic follicles, DOMESTIC ANIMAL ENDOCRINOLOGY, 29(4), 634-645, 2005
  111. Enzyme immunoassay of progesterone in the feces from beef cattle to monitor the ovarian cycle, ANIMAL REPRODUCTION SCIENCE, 87(42737), 1-10, 2005
  112. Gonadotropin-induced Delta 14-reductase and Delta 7-reductase gene expression in cumulus cells during meiotic resumption of porcine oocytes, ENDOCRINOLOGY, 146(1), 186-194, 2005
  113. Pregnancy diagnosis based on the fecal progesterone concentration in beef and dairy heifers and beef cows, ANIMAL REPRODUCTION SCIENCE, 90(42798), 211-218, 2005
  114. Immunolocalization of von Willebrand factor and vascular endothelial growth factor during follicular atresia in the swamp buffalo ovary, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 51(4), 419-426, 2005
  115. Apoptosis in the antral follicles of swamp buffalo and cattle ovary: TUNEL and caspase-3 histochemistry, REPRODUCTION IN DOMESTIC ANIMALS, 40(2), 111-116, 2005
  116. Expressions of antimicrobial peptide gallinacin-1,-2 and -3 mRNAs in the oviduct of laying hens., Journal of Poultry Science, 42(4), 337-345, 20051025
  117. Direct enzyme immunoassay of fecal estrone derivatives in dairy cows. :, Animal Science Journal, 76(3), 203-207, 20050701
  118. Characteristics of cryopreserved spermatozoa from a holstein friesian bull thawed at different temperature., Journal of Internatinal Development and Cooperation, 12(1), 107-110, 20050401
  119. Pregnancy diagnosis in miniature pig by direct ELISA of oestrone derivatives in faeces, REPRODUCTION IN DOMESTIC ANIMALS, 39(1), 48-51, 2004
  120. Cell proliferation in the atretic follicles of buffalo and cattle ovary, REPRODUCTION IN DOMESTIC ANIMALS, 39(6), 405-409, 2004
  121. Determination of estrous cycle and pregnancy diagnosis in miniature pig by measuring plasma progesterone concentrations., 1, 25-28, 20040301
  122. Atresia ani with diphallus and separate scrota in a calf: a case report, THERIOGENOLOGY, 61(42924), 1205-1213, 2004
  123. Changes in the thecal vasculature during follicular atresia in the ovary of swamp buffalo, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 50(3), 315-321, 2004
  124. Incidence of silent ovulation in dairy cows during post partum period, DEUTSCHE TIERARZTLICHE WOCHENSCHRIFT, 111(1), 35-38, 2004
  125. Phosphatidylinositol 3-kinase in cumulus cells is responsible for both suppression of spontaneous maturation and induction of gonadotropin-stimulated maturation of porcine oocytes, JOURNAL OF ENDOCRINOLOGY, 179(1), 25-34, 2003
  126. Distribution of cytochrome p450-side chain cleavage in the theca interna layers of bovine small antral and cystic follicles, REPRODUCTION IN DOMESTIC ANIMALS, 38(5), 405-409, 2003
  127. Plasma concentration of estrone sulfate during pregnancy in different breeds of Japanese beef cattle, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 49(5), 369-374, 2003
  128. Effects of body condition score in cows peripartum on the onset of postpartum ovarian cyclicity and conception rates after ovulation synchronization/fixed-time artificial insemination, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 49(5), 381-388, 2003
  129. Immunohistochemical localization of 3 beta-hydroxysteroid dehydrogenase in the granulosa and theca interna layers of bovine cystic follicles, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 49(3), 227-233, 2003
  130. Luteinizing hormone receptor formation in cumulus cells surrounding porcine oocytes and its role during meiotic maturation of porcine oocytes, BIOLOGY OF REPRODUCTION, 68(4), 1142-1149, 2003
  131. Effects of estrone sulfate injection on reproductive functions in male Japanese quail., Journal of Poultry Science, 40, 243-247, 20031001
  132. Direct enzyme immunoassay of progesterone in bovine plasma., Anim Sci J, 74, 369-373, 20031001
  133. Direct enzyme immunoassay of estrone sulfate in the plasma of cattle, J Reprod Dev, 48(1), 75-78, 20020201
  134. Direct enzyme immunoassay of estrone sulfate in the plasma of cattle, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 48(1), 75-78, 2002
  135. Survival of boar spermatozoa frozen in diluents of varying osmolality, THERIOGENOLOGY, 56(3), 447-458, 20010801
  136. Changes in the Localization of Immunoreactive von Willebrand Factor in Microvascular Network of Bovine Ovarian Follicles during Atresia, Animal Science Journal, 72(6), 473-482, 20011101
  137. An in situ hybridization study of the effects of artificial insemination on the localization of cells expressing MHC class II mRNA in the chicken oviduct, Reproduction, 122(4), 581‐586, 20011001
  138. An in situ hybridization study of the effects of artificial insemination on the localization of cells expressing MHC class II mRNA in the chicken oviduct, REPRODUCTION, 122(4), 581-586, 2001
  139. Effect of the Factor inhibiting germinal vesicle breakdown on the disruption of gap junctions and cumulus expansion of pig cumulus-oocyte complexes cultured in vitro, REPRODUCTION, 121(2), 249-257, 2001
  140. Immunocytochemical study of cell proliferation in the cystic ovarian follicles in cows, THERIOGENOLOGY, 54(7), 1159-1169, 20001015
  141. Localization of apoptotic cells in the cystic ovarian follicles of cows: A DNA-end labeling histochemical study, THERIOGENOLOGY, 53(4), 897-904, 20000301
  142. Involvement of meiotic resumption in the disruption of gap junctions between cumulus cells attached to pig oocytes., Journal of Reproduction and Fertility, 113, 167-172, 1998
  143. Cumulus cells suppress meiotic progression in pig oocytes cultured in vitro., Theriogenology, 45, 1479-1489, 1996

Invited Lecture, Oral Presentation, Poster Presentation

  1. Effect of oral administration of colostrum on the antimicrobial peptides concentration in goat milk., Isobe N, Kubo K, Ueno K, Nii T, Yoshimura Y, The 8th International Seminar on Tropical Animal Production (ISTAP) , 2019/92, Without Invitation, English, The 8th International Seminar on Tropical Animal Production (ISTAP) , Yogyakarta, Indonesia
  2. Different concentration of antimicrobial components in bovine milk infected with different pathogens, Isobe N, Kawai K, Shinozuka Y, Purba YF, Nii T, Yoshimura Y, The 30th World Buiatrics Congress, 2018/82, Without Invitation, English, World Buiatrics, Sapporo Convention Center, Sapporo
  3. Effect of mastitis on the reproductive functions in pregnant goat., Isobe N, Kodama N, Nii T, Yoshimura Y, 18th AAAP Congress., 2018/81, Without Invitation, English, Asian Austrasian Animal Production, Sarawak, Malaysia
  4. Effect of tentative cessation of milking for 3 days on innate immune components in goat milk, Naoki Isobe, Jo Ueda, Yukinori Yoshimura, 2018 Annual Meeting of the American Dairy Science Association, 2018/07/25, Without Invitation, English, American Dairy Science Association, Knoxville,Tennessee, USA
  5. innate immune function for the prevention of mastitis, Naoki Isobe, 2017/10/06, With Invitation, Japanese
  6. mastitis and innate immunity, Naoki Isobe, 2017/05/27, With Invitation, Japanese
  7. Cessation of milking and antimicrobial peptides, Naoki Isobe, Bovine Mastitis Research, 2017/04/15, With Invitation, Japanese
  8. changes in vial bacterial count in mastitic milk and its reason, Naoki Isobe, 2017/03/07, With Invitation, Japanese
  9. Mastitis and innate immunity, Naoki Isobe, 2016/11/05, With Invitation, Japanese, Rakuno Gakuen University
  10. Effect of steroid hormone on innate immune response induced by Staphylococcus aureus in mammary gland of goat., Isobe N, Kuwahara K, Yoshimura Y, The 17th AAAP Animal Science Congress, 2016/08/22, Without Invitation, English, AAAP, Kyusyusangyo University
  11. Changes in pathogen number during preservation of milk derived from mastitic dairy cows., Isobe N, Hisaeda K, Koshiishi T, Watanabe M, Miyake H, Yoshimura Y, The 6th International Seminar on Tropical Animal Production (ISTAP), 2015/10/20, Without Invitation, English, Yogyakarta, Indonesia
  12. Innate immunity in mammary gland, Naoki Isobe, 2015/02/15, With Invitation, Japanese
  13. Association of reproductive performance with somatic cell count in milk of dairy cows., Isobe N, Iwamoto C, Yoshimura Y, The 16th AAAP Animal Science Congress, 2014/10/10, Without Invitation, English, Yogyakarta, Indonesia
  14. Antimicrobial factors in milk, Naoki Isobe, 2014/08/22, With Invitation, Japanese, NOSAI

Awards

  1. 2018/03/28, The 2018 Award for Excellence in Reviewing for Animal Science Journal in 2017, 公益社団法人日本畜産学会編集委員長
  2. 2015/03/28, Animal Science Journal Reviewers Award (Reviewers賞), 日本畜産学会編集委員会

External Funds

Acceptance Results of Competitive Funds

  1. Prevention of calf diarrhea by utilizing antimicrobial peptide in colostrum, 2017, 2017
  2. KAKENHI, 2015, 2017
  3. 2015
  4. KAKENHI, 2012, 2014
  5. 2012
  6. 2011, 2013
  7. KAKENHI, Studies on the mechanism of antimicrobial peptide synthesis for a strategy to enhance the innate immunity in hen oviduct, 2009, 2012
  8. KAKENHI, Development of Fertility Improvement Program in Dairy Cows based on Postpartum Reproductive Monitoring System, 2008, 2010
  9. KAKENHI, 2006, 2008
  10. KAKENHI, 2006, 2008
  11. KAKENHI, 2004, 2005
  12. KAKENHI, Environmental pollution by sex steroids and assessment for their effects on animals and plants, 2003, 2004
  13. KAKENHI, 2000, 2000
  14. KAKENHI, Studies on potential of domestic ruminants in tropical Asia, 2000, 2003
  15. KAKENHI, Feto-Placental Function during Pregnancy and the Calf Viability, 2000, 2001

Social Activities

History as Peer Reviews of Academic Papers

  1. 2014, Animal Science Journal, Others
  2. 2014, Journal of Reproduction and Development
  3. 2014, Domestic Animal Endocrinology
  4. 2014, BMC Veterinary
  5. 2014, Current Protein & Peptide Science
  6. Veterinary Research