KOJI IKEGAMI

Last Updated :2019/12/02

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Medicine), ., Professor
E-mail
k-ikegamihiroshima-u.ac.jp

Basic Information

Major Professional Backgrounds

  • 2019/04/01, Hiroshima University, Graduate School of Biomedical & Health Sciences , Professor
  • 2018/04/01, 2019/03/31, Hiroshima University, Graduate School of Biomedical & Health Sciences , Professor
  • 2011/08/01, 2018/03/31, Hamamatsu University School of Medicine, Associate Professor
  • 2008/08/01, 2011/07/31, Hamamatsu University School of Medicine, Assistant Professor (Special Appointment)
  • 2007/04/01, 2008/07/31, Mitsubishi Kagaku Institute of Life Sciences, Research associate
  • 2004/04/01, 2007/03/31, Mitsubishi Kagaku Institute of Life Sciences, Postdoctoral Fellow
  • 2001/04/01, 2004/03/31, JSPS, Special Researcher

Educational Backgrounds

  • Hokkaido University, Graduate School of Science, Doctor Course, Division of Biological Sciences, Japan, 2001/04, 2004/03
  • Hokkaido University, Graduate School of Science, Master Course, Division of Biological Sciences, Japan, 1999/04, 2001/03
  • HOKKAIDO UNIVERSITY, Faculty of Science, Japan, 1995/04, 1999/03

Academic Degrees

  • PhD, Hokkaido University

Research Fields

  • Medicine,dentistry, and pharmacy;Basic medicine;General anatomy (including histology / embryology)
  • Biology;Biological Science;Cell biology

Research Keywords

  • cytoskeleton
  • cilia and flagella
  • extracellular vesicles

Affiliated Academic Societies

Educational Activity

Course in Charge

  1. 2019, Liberal Arts Education Program1, 3Term, Anatomy for understanding human being I
  2. 2019, Liberal Arts Education Program1, 4Term, Anatomy for understanding human being II
  3. 2019, Liberal Arts Education Program1, 1Term, Foundation biology for life science
  4. 2019, Liberal Arts Education Program1, 1Term, Foundation biology for life science
  5. 2019, Undergraduate Education, 2Term, Practice in Anatomy Part I
  6. 2019, Undergraduate Education, Second Semester, Practice in Anatomy Part II
  7. 2019, Undergraduate Education, 2Term, Introduction for medical research
  8. 2019, Undergraduate Education, 4Term, Medical Neuroscience I
  9. 2019, Undergraduate Education, Intensive, Practice for medical research
  10. 2019, Undergraduate Education, First Semester,
  11. 2019, Undergraduate Education, 2Term, Practice in anatomy part I
  12. 2019, Undergraduate Education, Second Semester, Practice in anatomy part II
  13. 2019, Liberal Arts Education Program1, 1Term, Introductory Seminar for First-Year Students
  14. 2019, Graduate Education (Master's Program) , First Semester, Human anatomy
  15. 2019, Graduate Education (Master's Program) , Second Semester, Practice in human gross anatomy
  16. 2019, Graduate Education (Master's Program) , First Semester, Seminar on Anatomy and Developmental Biology
  17. 2019, Graduate Education (Master's Program) , Second Semester, Seminar on Anatomy and Developmental Biology
  18. 2019, Graduate Education (Master's Program) , First Semester, Research on Anatomy and Developmental Biology
  19. 2019, Graduate Education (Master's Program) , Second Semester, Research on Anatomy and Developmental Biology
  20. 2019, Graduate Education (Doctoral Program) , First Semester, Advanced seminar on Anatomy and Developmental Biology
  21. 2019, Graduate Education (Doctoral Program) , Second Semester, Advanced seminar on Anatomy and Developmental Biology
  22. 2019, Graduate Education (Master's Program) , First Semester, Research
  23. 2019, Graduate Education (Master's Program) , Second Semester, Research
  24. 2019, Graduate Education (Doctoral Program) , First Semester, Advanced research on Anatomy and Developmental Biology
  25. 2019, Graduate Education (Doctoral Program) , Second Semester, Advanced research on Anatomy and Developmental Biology
  26. 2019, Graduate Education (Master's Program) , First Semester, Seminar
  27. 2019, Graduate Education (Master's Program) , Second Semester, Seminar
  28. 2019, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar
  29. 2019, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar
  30. 2019, Graduate Education (Doctoral Program) , First Semester, Advanced Research
  31. 2019, Graduate Education (Doctoral Program) , Second Semester, Advanced Research
  32. 2019, Graduate Education (Master's Program) , First Semester, Human Anatomy
  33. 2019, Graduate Education (Master's Program) , Second Semester, Practice in Human Gross Anatomy
  34. 2019, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Anatomy and Developmental Biology
  35. 2019, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Anatomy and Developmental Biology
  36. 2019, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Anatomy and Developmental Biology
  37. 2019, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Anatomy and Developmental Biology

Research Activities

Academic Papers

  1. Live cell imaging of dynamic behaviors of motile cilia and primary cilium, MICROSCOPY, 68(2), 99-110, 2019
  2. An in vitro model of region-specific rib formation in chick axial skeleton: Intercellular interaction between somite and lateral plate cells, Mechanisms of Development, 159, 103568, 2019
  3. Preferential Incorporation of Administered Eicosapentaenoic Acid Into Thin-Cap Atherosclerotic Plaques, Arteriosclerosis, Thrombosis, and Vascular Biology, 39(9), 1802-1816, 2019
  4. Three-dimensional tracking of microbeads attached to the tip of single isolated tracheal cilia beating under external load, Scientific Reports, 8, 15562, 2018
  5. UBL3 modification influences protein sorting to small extracellular vesicles, Nature Communications, 9, 3936, 2018
  6. Role of caveolin-1 in hepatocellular carcinoma arising from non-alcoholic fatty liver disease, Cancer Science, 109(8), 2401-2411, 2018
  7. Environmental responsiveness of tubulin glutamylation in sensory cilia is regulated by the p38 MAPK pathway, SCIENTIFIC REPORTS, 8(1), 8392, 2018
  8. Stearate-to-palmitate ratio modulates endoplasmic reticulum stress and cell apoptosis in non-B non-C hepatoma cells, Cancer Science, 109(4), 1110-1120, 2018
  9. A targeted next-generation sequencing panel reveals novel mutations in Japanese patients with primary ciliary dyskinesia, Auris Nasus Larynx, 45(3), 585-591, 2018
  10. Impaired airway mucociliary function reduces antigen-specific IgA immune response to immunization with a claudin-4-targeting nasal vaccine in mice, Scientific Reports, 8(1), 2904, 2018
  11. Proper cytoskeletal architecture beneath the plasma membrane of red blood cells requires Ttll4, Molecular Biology of the Cell, 28(4), 535-544, 2017
  12. Increase in α-tubulin modifications in the neuronal processes of hippocampal neurons in both kainic acid-induced epileptic seizure and Alzheimer's disease, Scientific Reports, 7, 40205, 2017
  13. Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision, Cell, 168(1-2), 264-279, 2017
  14. Ttll9-/- mice sperm flagella show shortening of doublet 7, reduction of doublet 5 polyglutamylation and a stall in beating, Journal of Cell Science, 129(14), 2757-2766, 2016
  15. Accumulation of arachidonic acid-containing phosphatidylinositol at the outer edge of colorectal cancer, Scientific Reports, 6, 29935, 2016
  16. Direct profiling of the phospholipid composition of adult Caenorhabditis elegans using whole-body imaging mass spectrometry, Analytical and Bioanalytical Chemistry, 407(25), 7589-7602, 2015
  17. Mechanical Stimulation by Postnasal Drip Evokes Cough, PloS One, 10(11), e0141823, 2015
  18. Single-cell time-of-flight secondary ion mass spectrometry reveals that human breast cancer stem cells have significantly lower content of palmitoleic acid compared to their counterpart non-stem cancer cells, Biochimie, 107(A), 73-77, 2014
  19. Single cell lipidomics of SKBR‐3 breast cancer cells by using time‐of‐flight secondary‐ion mass spectrometry, Surface and Interface Analysis, 46(S1), 181-184, 2014
  20. Application of 2,5‐dihydroxyacetophenone with sublimation provides efficient ionization of lipid species by atmospheric pressure matrix‐assisted laser desorption/ionization imaging mass spectrometry, Surface and Interface Analysis, 46(12-13), 1219-1222, 2014
  21. Adventitial vasa vasorum arteriosclerosis in abdominal aortic aneurysm, PloS One, 8(2), e57398, 2013
  22. Lysophosphatidylcholine acyltransferase 1 altered phospholipid composition and regulated hepatoma progression, Journal of Hepatology, 59(2), 292-299, 2013
  23. Cytosolic carboxypeptidase 5 removes α- and γ-linked glutamates from tubulin, The Journal of Biological Chemistry, 288(42), 30445-30453, 2013
  24. Cytosolic carboxypeptidase 1 is involved in processing α- and β-tubulin, The Journal of Biological Chemistry, 287(9), 6530-6517, 2012
  25. Ciliary and flagellar structure and function--their regulations by posttranslational modifications of axonemal tubulin, International Review of Cell and Molecular Biology, 294, 133-170, 2012
  26. Axonal gradient of arachidonic acid-containing phosphatidylcholine and its dependence on actin dynamics, The Journal of Biological Chemistry, 287(8), 5290-5300, 2012
  27. CEP41 is mutated in Joubert syndrome and is required for tubulin glutamylation at the cilium, Nature Genetics, 44(2), 193-199, 2012
  28. Submolecular-scale imaging of α-helices and C-terminal domains of tubulins by frequency modulation atomic force microscopy in liquid, Biophysical Journal, 101(5), 1270-1276, 2011
  29. SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5Kgamma Activity through Deacetylation of Specific Lysine Residues in Mammals, PloS One, 5(7), e11755, 2010
  30. Detection of characteristic distributions of phospholipid head groups and fatty acids on neurite surface by time-of-flight secondary ion mass spectrometry, Medical Molecular Morphology, 43(3), 158-164, 2010
  31. Imaging mass spectrometry of gastric carcinoma in formalin-fixed paraffin-embedded tissue microarray, Cancer Science, 101(1), 267-273, 2010
  32. Unique post-translational modifications in specialized microtubule architecture, Cell Structure and Function, 35(1), 15-22, 2010
  33. Developments and applications of mass microscopy, Medical Molecular Morphology, 43(1), 1-5, 2010
  34. Identification of tubulin deglutamylase among Caenorhabditis elegans and mammalian cytosolic carboxypeptidases (CCPs), The Journal of Biological Chemistry, 285(30), 22936-22941, 2010
  35. Tubulin polyglutamylation is essential for airway ciliary function through the regulation of beating asymmetry, Proceedings of the National Academy of Sciences of the United States of America, 107(23), 10490-10495, 2010
  36. Imaging of lipids in cultured mammalian neurons by matrix assisted laser/desorption ionization and secondary ion mass spectrometry, Surface and Interface Analysis, 42(10-11), 1606-1611, 2010
  37. Recombinant mammalian tubulin polyglutamylase TTLL7 performs both initiation and elongation of polyglutamylation on beta-tubulin through a random sequential pathway, Biochemistry, 48(5), 1084-1093, 2009
  38. TTLL10 can perform tubulin glycylation when co-expressed with TTLL8, FEBS Letters, 583(12), 1957-1963, 2009
  39. TTLL10 is a protein polyglycylase that can modify nucleosome assembly protein 1, FEBS Letters, 582(7), 1129-1134, 2008
  40. Transmembrane and ubiquitin-like domain-containing protein 1 (Tmub1/HOPS) facilitates surface expression of GluR2-containing AMPA receptors, PloS One, 3(7), e2809, 2008
  41. Enhancement of Trk signaling pathways by the cholestane amide conjugate MCC-257, Journal of Pharmacological Sciences, 108(1), 131-134, 2008
  42. Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function, Proceedings of the National Academy of Sciences of the United States of America, 104(9), 3213-3218, 2007
  43. TTLL7 is a mammalian beta-tubulin polyglutamylase required for growth of MAP2-positive neurites, The Journal of Biological Chemistry, 281(41), 30707-30716, 2006
  44. Hypo-osmotic shock induces nuclear export and proteasome-dependent decrease of UBL5, Biochemical and Biophysical Research Communications, 350(3), 610-615, 2006
  45. N-alpha-p-tosyl-L-lysine chloromethyl ketone (TLCK) suppresses neuritic degeneration caused by different experimental paradigms including in vitro Wallerian degeneration, Brain Research, 1030(1), 81-93, 2004
  46. Non-apoptotic neurite degeneration in apoptotic neuronal death: Pivotal role of mitochondrial function in neurites, Neuroscience, 122(3), 617-626, 2003
  47. Membrane depolarization-mediated survival of sympathetic neurons occurs through both phosphatidylinositol 3-kinase- and CaM kinase II-dependent pathways, Brain Research, 866(1-2), 218-226, 2000

Publications such as books

  1. Analysis of Post-Translational Modifications and Proteolysis in Neuroscience, Humana Press, 2016, English, 1493934708
  2. Imaging mass spectrometry - protocols for mass microscopy, Springer, 2010, English, 4431094245

Invited Lecture, Oral Presentation, Poster Presentation

  1. Lipoquality-mediated regulation of primary cilia dynamics, Ikegami Koji, Setou Mitsutoshi, International Symposium on Imaging Frontier 2017, 2017/07/09, Without Invitation, Tokyo
  2. Evidence for release of ciliary components into extracellular fluid, Ikegami Koji, Setou Mitsutoshi, The 28th CDB meeting, 2016/11/29, Without Invitation, Kobe
  3. Release of cilia components into extracellular space, Ikegami Koji, Ireland – Japan Joint Seminar, 2016/10/12, With Invitation, Tokyo
  4. Posttranslational polymodifications of tubulin and other Targets, Ikegami Koji, Albert Einstein College of Medicine Special Seminar, 2013/07/01, Without Invitation, New York
  5. Analysis of Polymodification-performing Enzymes with Raster Image Correlation Spectroscopy, Ikegami Koji, IMB-Olympus Microscopy Suite Opening Ceremony, Live Cell Imaging Seminer, 2011/05, With Invitation, IMB/Olympus, Singapore
  6. Tubulin polyglutamylation for structural and functional asymmetry of cilia, Ikegami Koji, Sato Shobu, Nakamura Kenji, Setou Mitsutoshi, ASCB/JSCB/RIKEN CDB Meeting, 2009/09, Without Invitation, ASCB, Kyoto
  7. Loss of tubulin polyglutamylation is associated with a lack of central apparatus in tracheacilia axoneme, dysregulated cilia beating, and severe ceaseless cough in TTLL1-deficient mouse, Ikegami Koji, Sato Shobu, Setou Mitsutoshi, 47th ASCB Annual Meeting, 2007/12, Without Invitation, ASCB, Washington DC