Takashi Yamamoto

Last Updated :2019/11/01

Affiliations, Positions
Graduate School of Integrated Sciences for Life, ., Professor
E-mail
tybighiroshima-u.ac.jp
Other Contact Details
1-3-1,Kagamiyama,Higashi-Hiroshima, Japan
TEL : (+81)824-24-7446 FAX : (+81)824-24-7498

Basic Information

Major Professional Backgrounds

  • 1992/08/01, 2002/05/31, Kumamoto University, Research Associate
  • 2002/06/01, 2003/10/31, Hiroshima University, Graduate School of Science , Lecturer
  • 2003/11/01, 2004/03/31, Hiroshima University, Graduate School of Science , Assistant Professor
  • 2004/04/01, 2019/03/31, Hiroshima University, Graduate School of Science , Professor
  • 2014/04/01, 2020/03/31, Tottori University, Visiting professor
  • 2014/04/01, 2020/03/31, Kumamoto University, Visiting professor
  • 2017/04/01, 2020/03/31, Collaborative Research Laboratory for Next Generation Automobile Technology, Professor
  • 2019/02/01, 2020/03/31, Genome Editing Innovation Center, Director of the center

Educational Backgrounds

  • Hiroshima University, Graduate School, Division of Natural Science, Japan, 1991/04, 1992/07
  • Hiroshima University, Graduate School, Division of Natural Science, Japan, 1989/04, 1991/03
  • Hiroshima University, Faculty of Science, Japan, 1985/04, 1989/03

Academic Degrees

  • Doctor of Science, Hiroshima University
  • Master of Science, Hiroshima University

Educational Activity

  • 【Bachelor Degree Program】School of Science : Biological Sciences
  • 【Master's Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Mathematical and Life Sciences
  • 【Master's Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biomedical Science
  • 【Doctoral Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Mathematical and Life Sciences
  • 【Doctoral Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biomedical Science

Research Fields

  • Biological Sciences;Genome science;Genome biology
  • Biological Sciences;Genome science;System genome science
  • Biology;Biological Science;Developmental biology

Research Keywords

  • Genome Editing
  • TALEN, CRISPR-Cas, ZFN
  • echinoderm
  • development
  • noise
  • gene regulatory network

Affiliated Academic Societies

  • The Japanese Society for Genome Editing, 2016

Educational Activity

Course in Charge

  1. 2019, Liberal Arts Education Program1, 3Term, Introduction to Biology
  2. 2019, Undergraduate Education, 4Term, Introduction to Biological Sciences B
  3. 2019, Undergraduate Education, 2Term, Advanced Biology
  4. 2019, Undergraduate Education, 3Term, Developmental Biology A
  5. 2019, Undergraduate Education, Second Semester, Seminar for Molecular Genetics
  6. 2019, Undergraduate Education, First Semester, Special Study for Graduation
  7. 2019, Undergraduate Education, Second Semester, Special Study for Graduation
  8. 2019, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
  9. 2019, Graduate Education (Master's Program) , First Semester, Introduction to Life Sciences
  10. 2019, Graduate Education (Master's Program) , First Semester, Special Research for Mathematical and Life Sciences
  11. 2019, Graduate Education (Master's Program) , Second Semester, Special Research for Mathematical and Life Sciences
  12. 2019, Graduate Education (Master's Program) , First Semester, Seminar of Molecular Genetics
  13. 2019, Graduate Education (Master's Program) , Second Semester, Seminar of Molecular Genetics
  14. 2019, Graduate Education (Doctoral Program) , First Semester, Special Research for Mathematical and Life Sciences
  15. 2019, Graduate Education (Doctoral Program) , Second Semester, Special Research for Mathematical and Life Sciences
  16. 2019, Graduate Education (Doctoral Program) , First Semester, Seminar of Molecular Genetics
  17. 2019, Graduate Education (Doctoral Program) , Second Semester, Seminar of Molecular Genetics
  18. 2019, Graduate Education (Master's Program) , 1Term, Introduction to Life Science
  19. 2019, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Mathematical and Life Sciences
  20. 2019, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Mathematical and Life Sciences
  21. 2019, Graduate Education (Master's Program) , 1Term, Exercises in Life Science A
  22. 2019, Graduate Education (Master's Program) , 2Term, Exercises in Life Science A
  23. 2019, Graduate Education (Master's Program) , 3Term, Exercises in Life Science B
  24. 2019, Graduate Education (Master's Program) , 4Term, Exercises in Life Science B
  25. 2019, Graduate Education (Master's Program) , OutOfTerm(1st), Basic Training of Genome Editing
  26. 2019, Graduate Education (Master's Program) , 2Term, Topical Seminar in Life Science A
  27. 2019, Graduate Education (Master's Program) , 4Term, Topical Seminar in Life Science B
  28. 2019, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences
  29. 2019, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar A
  30. 2019, Graduate Education (Master's Program) , 1Term, Advanced Technologies for Life Science
  31. 2019, Graduate Education (Master's Program) , 1Term, Exercises in Biomedical Science A
  32. 2019, Graduate Education (Master's Program) , 2Term, Exercises in Biomedical Science A
  33. 2019, Graduate Education (Master's Program) , 3Term, Exercises in Biomedical Science B
  34. 2019, Graduate Education (Master's Program) , 4Term, Exercises in Biomedical Science B
  35. 2019, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biomedial Science
  36. 2019, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar C
  37. 2019, Graduate Education (Master's Program) , OutOfTerm(1st), Ethics of Genome Editing Research
  38. 2019, Graduate Education (Master's Program) , 2Term, Basics and Practical Applications of Genome Editing

Research Activities

Academic Papers

  1. Biased genome editing using the local accumulation of DSB repair molecules system, NATURE COMMUNICATIONS, 9, AUG 16 2018
  2. Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9, NATURE COMMUNICATIONS, 5, 201411
  3. Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system, SCIENTIFIC REPORTS, 4, 20140623
  4. Efficient TALEN construction and evaluation methods for human cell and animal applications, GENES TO CELLS, 18(4), 315-326, 201304
  5. Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity, SCIENTIFIC REPORTS, 3, 20131129
  6. Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(27), 10915-10920, 20120703
  7. Humanized UGT2 and CYP3A transchromosomic rats for improved prediction of human drug metabolism, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 116(8), 3072-3081, 20190219
  8. KLF1 mutation E325K induces cell cycle arrest in erythroid cells differentiated from congenital dyserythropoietic anemia patient-specific induced pluripotent stem cells, EXPERIMENTAL HEMATOLOGY, 73, 25-37, 201905
  9. Nucleotide receptor P2RY4 is required for head formation via induction and maintenance of head organizer in Xenopus laevis, DEVELOPMENT GROWTH & DIFFERENTIATION, 61(2), 186-197, 201902
  10. Increased seizure sensitivity, emotional defects and cognitive impairment in PHD finger protein 24 (Phf24)-null rats, BEHAVIOURAL BRAIN RESEARCH, 369, 20190902
  11. Elucidation of secondary alcohol metabolism in Starmerella bombicola and contribution of primary alcohol oxidase FAO1, FEMS YEAST RESEARCH, 19(2), 201903
  12. Anephrogenic phenotype induced by SALL1 gene knockout in pigs, SCIENTIFIC REPORTS, 9, 20190529
  13. Loss of HCN1 subunits causes absence epilepsy in rats, BRAIN RESEARCH, 1706, 209-217, 20190301
  14. PDIP38/PolDIP2 controls the DNA damage tolerance pathways by increasing the relative usage of translesion DNA synthesis over template switching, PLOS ONE, 14(3), 20190306
  15. Microhomology-assisted scarless genome editing in human iPSCs, NATURE COMMUNICATIONS, 9, 20180305
  16. Three-Component Repurposed Technology for Enhanced Expression: Highly Accumulable Transcriptional Activators via Branched Tag Arrays, CRISPR JOURNAL, 1(5), 337-347, 201810
  17. PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetro-oligomerization through interaction with Bid in human colon cancer, CELL DEATH DISCOVERY, 4, 20180208
  18. Identification of a cell-penetrating peptide applicable to a protein-based transcription activator-like effector expression system for cell engineering, BIOMATERIALS, 173, 11-21, AUG 2018
  19. MET Activation by a Macrocyclic Peptide Agonist that Couples to Biological Responses Differently from HGF in a Context-Dependent Manner, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 19(10), 201810
  20. Exploration of genetic basis underlying individual differences in radiosensitivity within human populations using genome editing technology, JOURNAL OF RADIATION RESEARCH, 59, 201804
  21. Generation of and characterization of anti-IL-11 antibodies using newly established Il11-deficient mice, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 505(2), 453-459, 20181028
  22. Differential micronucleus frequency in isogenic human cells deficient in DNA repair pathways is a valuable indicator for evaluating genotoxic agents and their genotoxic mechanisms, ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 59(6), 529-538, 201807
  23. Replication stress induces accumulation of FANCD2 at central region of large fragile genes, NUCLEIC ACIDS RESEARCH, 46(6), 2932-2944, APR 6 2018
  24. Electroporation-mediated genome editing in vitrified/warmed mouse zygotes created by IVF via ultra-superovulation, EXPERIMENTAL ANIMALS, 67(4), 535-543, 201812
  25. Cas9 ribonucleoprotein complex allows direct and rapid analysis of coding and noncoding regions of target genes in Pleurodeles waltl development and regeneration, DEVELOPMENTAL BIOLOGY, 443(2), 127-136, 20181115
  26. Acceleration of cancer science with genome editing and related technologies, CANCER SCIENCE, 109(12), 3679-3685, 201812
  27. Unexpected heterogeneity derived from Cas9 ribonucleoprotein-introduced clonal cells at the HPRT1 locus, GENES TO CELLS, 23(4), 255-263, 201804
  28. Scleraxis is a transcriptional activator that regulates the expression of Tenomodulin, a marker of mature tenocytes and ligamentocytes, SCIENTIFIC REPORTS, 8, 20180216
  29. Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes, STEM CELL REPORTS, 11(3), 727-740, SEP 11 2018
  30. Functional analysis of thyroid hormone receptor beta in Xenopus tropicalis founders using CRISPR-Cas, BIOLOGY OPEN, 7(1), 201801
  31. HpBase: A genome database of a sea urchin, Hemicentrotus pulcherrimus, DEVELOPMENT GROWTH & DIFFERENTIATION, 60(3), 174-182, 201804
  32. Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 125(5), 599-605, 201805
  33. Cancer induction and suppression with transcriptional control and epigenome editing technologies, JOURNAL OF HUMAN GENETICS, 63(2), 187-194, 201802
  34. Cas9, Cpf1 and C2c1/2/3-What's next?, BIOENGINEERED, 8(3), 265-273, 2017
  35. Establishment of expanded and streamlined pipeline of PITCh knock-in - a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO, BIOENGINEERED, 8(3), 302-308, 2017
  36. Highly efficient biallelic genome editing of human ES/iPS cells using a CRISPR/Cas9 or TALEN system, NUCLEIC ACIDS RESEARCH, 45(9), 5198-5207, 20170519
  37. Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice, BIOLOGY OPEN, 6(5), 706-713, 20170515
  38. PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells, SCIENTIFIC REPORTS, 7, 20170703
  39. Germ cell regeneration-mediated, enhanced mutagenesis in the ascidian Ciona intestinalis reveals flexible germ cell formation from different somatic cells, DEVELOPMENTAL BIOLOGY, 423(2), 111-125, 20170315
  40. Participation of androgen and its receptor in sex determination of an amphibian species, PLOS ONE, 12(6), 20170605
  41. TALEN-mediated targeted editing of the GDE5 gene suppresses fibroblastic cell proliferation, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81(11), 2164-2167, 2017
  42. Dynamic changes in the interchromosomal interaction of early histone gene loci during development of sea urchin, JOURNAL OF CELL SCIENCE, 130(24), 4097-4107, DEC 2017
  43. Hox-mediated endodermal identity patterns pharyngeal muscle formation in the chordate pharynx, DEVELOPMENT, 144(9), 1629-1634, 20170501
  44. Clustered Xenopus keratin genes: A genomic, transcriptomic, and proteomic analysis, DEVELOPMENTAL BIOLOGY, 426(2), 384-392, 20170615
  45. Developmental changes in drug-metabolizing enzyme expression during metamorphosis of Xenopus tropicalis, JOURNAL OF TOXICOLOGICAL SCIENCES, 42(5), 605-613, 201710
  46. Establishment of pten knockout medaka with transcription activator-like effector nucleases (TALENs) as a model of PTEN deficiency disease, PLOS ONE, 12(10), 20171020
  47. Evaluation of ATM heterozygous mutations underlying individual differences in radiosensitivity using genome editing in human cultured cells, SCIENTIFIC REPORTS, 7, 20170720
  48. Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123(3), 287-293, 201703
  49. Modification of single-nucleotide polymorphism in a fully humanized CYP3A mouse by genome editing technology, SCIENTIFIC REPORTS, 7, 20171109
  50. MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems, Nature Protocols, 11, 118-133, 2016
  51. Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ, BMC GENOMICS, 17, 20161128
  52. Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice, BIOLOGY OPEN, 5(8), 1142-1148, 20160815
  53. Establishment of Functional Genomics Pipeline in Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9, HUMAN GENE THERAPY, 27(6), 436-450, 201606
  54. In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration, GENES TO CELLS, 21(4), 358-369, 201604
  55. C-Type Lectin Receptor DCAR Recognizes Mycobacterial Phosphatidyl-Inositol Mannosides to Promote a Th1 Response during Infection, IMMUNITY, 45(6), 1245-1257, 20161220
  56. Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation, JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 27(6), 1778-1791, 201606
  57. Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets, MOLECULAR REPRODUCTION AND DEVELOPMENT, 83(1), 61-70, 201601
  58. ALC1/CHD1L, a chromatin-remodeling enzyme, is required for efficient base excision repair, PLOS ONE, 12(11), 20171117
  59. Highly multiplexed CRISPR-Cas9-nuclease and Cas9-nickase vectors for inactivation of hepatitis B virus, GENES TO CELLS, 21(11), 1253-1262, 201611
  60. Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation, PLOS GENETICS, 12(1), 201601
  61. Involvement of aspartoacylase in tremor expression in rats, EXPERIMENTAL ANIMALS, 65(3), 293-301, 201607
  62. Systematic Cellular Disease Models Reveal Synergistic Interaction of Trisomy 21 and GATA1 Mutations in Hematopoietic Abnormalities, CELL REPORTS, 15(6), 1228-1241, 20160510
  63. Rapid and efficient analysis of gene function using CRISPR-Cas9 in Xenopus tropicalis founders, GENES TO CELLS, 21(7), 755-771, 201607
  64. Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing, CELL STEM CELL, 19(1), 127-138, 20160707
  65. Depdc5 knockout rat: A novel model of mTORopathy, NEUROBIOLOGY OF DISEASE, 89, 180-189, 201605
  66. Cilia play a role in breaking left-right symmetry of the sea urchin embryo, GENES TO CELLS, 21(6), 568-578, 201606
  67. Single-Cell-State Culture of Human Pluripotent Stem Cells Increases Transfection Efficiency, BIORESEARCH OPEN ACCESS, 5(1), 127-136, 201605
  68. Quadruple zebrafish mutant reveals different roles of Mesp genes in somite segmentation between mouse and zebrafish, DEVELOPMENT, 143(15), 2842-2852, 201608
  69. Transcriptional regulation of a horizontally transferred gene from bacterium to chordate, PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 283(1845), 20161228
  70. Functional consequence of fibulin-4 missense mutations associated with vascular and skeletal abnormalities and cutis laxa, MATRIX BIOLOGY, 56, 132-149, 201612
  71. Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells, STEM CELL REPORTS, 6(4), 496-510, 20160412
  72. Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish, GENES TO CELLS, 21(5), 492-504, 201605
  73. Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy, SCIENTIFIC REPORTS, 6, 20161124
  74. Report on the Conference on Transposition and Genome Engineering 2015 (TGE 2015): advancing cutting-edge genomics technology in the ancient city of Nara, GENES TO CELLS, 21(5), 392-395, 201605
  75. Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice, GENOME BIOLOGY, 16, 20150429
  76. The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos, PLOS ONE, 10(11), 20151118
  77. Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells, CELL STEM CELL, 17(2), 178-194, 20150806
  78. Simultaneous live imaging of the transcription and nuclear position of specific genes, NUCLEIC ACIDS RESEARCH, 43(19), 20151030
  79. Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 16(10), 23849-23866, 201510
  80. Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9, SCIENTIFIC REPORTS, 5, 20150609
  81. Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes, BMC BIOTECHNOLOGY, 15, 20150522
  82. Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish, SCIENTIFIC REPORTS, 5, 20150305
  83. Smarcal1 promotes double-strand-break repair by nonhomologous end-joining, NUCLEIC ACIDS RESEARCH, 43(13), 6359-6372, 20150727
  84. Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9, STEM CELL REPORTS, 5(1), 75-82, 20150714
  85. The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation, CELL REPORTS, 10(5), 664-673, 20150210
  86. Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus, BIOTECHNOLOGY AND BIOENGINEERING, 112(7), 1335-1342, 201507
  87. Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway, JOURNAL OF CELL BIOLOGY, 211(4), 775-784, 20151123
  88. Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine, DEVELOPMENTAL BIOLOGY, 403(1), 43-56, 20150701
  89. A High Excision Potential of TALENs for Integrated DNA of HIV-Based Lentiviral Vector, PLOS ONE, 10(3), 20150317
  90. Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs, IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 51(9), 879-884, 201510
  91. Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9, STEM CELL REPORTS, 4(1), 143-154, 20150113
  92. Unliganded Thyroid Hormone Receptor alpha Regulates Developmental Timing via Gene Repression in Xenopus tropicalis, ENDOCRINOLOGY, 156(2), 735-744, 201502
  93. Epithelial DLD-1 Cells with Disrupted E-cadherin Gene Retain the Ability to Form Cell Junctions and Apico-basal Polarity, CELL STRUCTURE AND FUNCTION, 40(2), 79-94, 2015
  94. Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring the juxtamembrane region and plakophilins, JOURNAL OF BIOCHEMISTRY, 158(4), 339-353, 201510
  95. Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines, GENES TO CELLS, 20(12), 1059-1076, 201512
  96. Targeted gene correction of RUNX1 in induced pluripotent stem cells derived from familial platelet disorder with propensity to myeloid malignancy restores normal megakaryopoiesis, EXPERIMENTAL HEMATOLOGY, 43(10), 849-857, 201510
  97. TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS ( MVA) syndrome, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(4), 1461-1466, 20140128
  98. EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step, JOURNAL OF CELL BIOLOGY, 206(3), 347-356, 20140804
  99. FAST-id system for enrichment of cells with TALEN-induced mutations and large deletions, GENES TO CELLS, 19(5), 419-431, 201405
  100. Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells, SCIENTIFIC REPORTS, 4, 20141120
  101. Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems, SCIENTIFIC REPORTS, 4, 20140716
  102. Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing, EXPERIMENTAL ANIMALS, 63(3), 349-355, 201407
  103. Simple knockout by electroporation of engineered endonucleases into intact rat embryos, SCIENTIFIC REPORTS, 4, 20141001
  104. Sterol Side Chain Reductase 2 Is a Key Enzyme in the Biosynthesis of Cholesterol, the Common Precursor of Toxic Steroidal Glycoalkaloids in Potato, PLANT CELL, 26(9), 3763-3774, 201409
  105. TALEN-induced gene knock out in Drosophila, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 86-91, 201401
  106. Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies, SCIENTIFIC REPORTS, 4, 20140827
  107. Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 108-114, 201401
  108. Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 115-121, 201401
  109. Screening Methods to Identify TALEN-Mediated Knockout Mice, EXPERIMENTAL ANIMALS, 63(1), 79-84, 201401
  110. Versatile strategy for isolating transcription activator-like effector nuclease-mediated knockout mutants in Caenorhabditis elegans, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 78-85, 201401
  111. Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex, BMC BIOTECHNOLOGY, 14, 20141118
  112. Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona, DEVELOPMENT, 141(2), 481-487, 20140115
  113. Targeted mutagenesis in sea urchin embryos using TALENs, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 92-97, 201401
  114. Germ Cell Mutations of the Ascidian Ciona intestinalis With TALE Nucleases, GENESIS, 52(5), 431-439, 201405
  115. Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes, SCIENTIFIC REPORTS, 3, 20130213
  116. Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome, DEVELOPMENTAL BIOLOGY, 375(1), 79-91, 20130301
  117. Efficient Targeted Mutagenesis in Medaka Using Custom-Designed Transcription Activator-Like Effector Nucleases, GENETICS, 193(3), 739-+, 201303
  118. High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos., Biol Open, 2, 448-452, 20130301
  119. Production of Sry knockout mouse using TALEN via oocyte injection, SCIENTIFIC REPORTS, 3, 20131105
  120. Quantitative assay for TALEN activity at endogenous genomic loci, BIOLOGY OPEN, 2(4), 363-367, 20130415
  121. The 3 ' UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin, DEVELOPMENTAL BIOLOGY, 377(1), 275-283, 20130501
  122. Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 54(5), 535-545, 201206
  123. Targeted disruption of exogenous EGFP gene in medaka using zinc-finger nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 54(5), 546-556, 201206
  124. Equarin is involved as an FGF signaling modulator in chick lens differentiation, DEVELOPMENTAL BIOLOGY, 368(1), 109-117, 20120801
  125. Nucleosome exclusion from the interspecies-conserved central AT-rich region of the Ars insulator, JOURNAL OF BIOCHEMISTRY, 151(1), 75-87, 201201
  126. Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases, NATURE COMMUNICATIONS, 3, 201208
  127. HpSumf1 is involved in the activation of sulfatases responsible for regulation of skeletogenesis during sea urchin development, DEVELOPMENT GENES AND EVOLUTION, 221(3), 157-166, 201108
  128. Implication of HpEts in Gene Regulatory Networks Responsible for Specification of Sea Urchin Skeletogenic Primary Mesenchyme Cells, ZOOLOGICAL SCIENCE, 27(8), 638-646, 201008
  129. Targeted mutagenesis in the sea urchin embryo using zinc-finger nucleases, GENES TO CELLS, 15(8), 875-885, 201008
  130. Dicer is Required for the Normal Development of Sea Urchin, Hemicentrotus pulcherrimus, ZOOLOGICAL SCIENCE, 27(6), 477-486, 201006
  131. HpSulf, a heparan sulfate 6-O-endosulfatase, is involved in the regulation of VEGF signaling during sea urchin development, MECHANISMS OF DEVELOPMENT, 127(3-4), 235-245, 2010
  132. Mammalian arylsulfatase A functions as a novel component of the extracellular matrix, CONNECTIVE TISSUE RESEARCH, 51(5), 388-396, 201010
  133. Suppressor of Hairless (Su(H)) is Required for Foregut Development in the Sea Urchin Embryo, ZOOLOGICAL SCIENCE, 26(10), 686-690, 200910
  134. Role of the Nanos Homolog During Sea Urchin Development, DEVELOPMENTAL DYNAMICS, 238(10), 2511-2521, 200910
  135. Analysis of cis-regulatory elements controlling spatio-temporal expression of T-brain gene in sea urchin, Hemicentrotus pulcherrimus, MECHANISMS OF DEVELOPMENT, 125(1-2), 2-17, 2008
  136. RSF governs silent chromatin formation via histone H2Av replacement, PLOS GENETICS, 4(2), 200802
  137. The Ars insulator facilitates I-SceI meganuclease-mediated transgenesis in the sea urchin embryo, DEVELOPMENTAL DYNAMICS, 237(9), 2475-2482, 200809
  138. DNA variations within the sea urchin Otx gene enhancer, FEBS LETTERS, 581(27), 5234-5240, 20071113
  139. Unichrom, a novel nuclear matrix protein, binds to the Ars insulator and canonical MARs, ZOOLOGICAL SCIENCE, 23(1), 9-21, 200601
  140. Developmental expression of HpNanos, the Hemicentrotus pulcherrimus homologue of nanos, GENE EXPRESSION PATTERNS, 6(5), 572-577, 200606
  141. Organ-specific and age-dependent expression of insulin-like growth factor-I (IGF-I) mRNA variants: IGF-IA and IB mRNAs in the mouse, ZOOLOGICAL SCIENCE, 22(9), 1011-1021, 200509
  142. The Otx binding site is required for the activation of HpOtxL mRNA expression in the sea urchin, Hemicentrotus pulcherrimus, Development= Growth and Differentiation, 46(1), 20040201
  143. Sexually dimorphic expression of a teleost homologue of Mullerian inhibiting substance during gonadal sex differentiation in Japanese flounder, Paralichthys olivaceus, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 322(2), 508-513, 20040917
  144. Identification of two teleost homologs of the Drosophila sex determination factor, transformer-2 in medaka (Oryzias latipes), MECHANISMS OF DEVELOPMENT, 121(7-8), 991-996, 200407
  145. The Otx binding site is required for the activation of HpOtxL mRNA expression in the sea urchin, Hemicentrotus pulcherrimus, DEVELOPMENT GROWTH & DIFFERENTIATION, 46(1), 61-69, 200402
  146. A new G-stretch-DNA-binding protein, Unichrom, displays cell-cycle-dependent expression in sea urchin embryos, DEVELOPMENT GROWTH & DIFFERENTIATION, 46(4), 335-341, 200408
  147. Utilization of a particle gun DNA introduction system for the analysis of cis-regulatory elements controlling the spatial expression pattern of the arylsulfatase gene (HpArs) in sea urchin embryos, DEVELOPMENT GENES AND EVOLUTION, 213(1), 44-49, 200302
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  150. T-brain homologue (HpTb) is involved in the archenteron induction signals of micromere descendant cells in the sea urchin embryo, DEVELOPMENT, 129(22), 5205-5216, 200211
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  153. Human recombinant stem cell factor promotes spermatogonial proliferation, but not meiosis initiation in organ culture of newt testis fragments, Biochemical and Biophysical Research Communications, 294(3), 695-699, 20020401
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  157. Mammalian follicle-stimulating hormone (FSH) and insulin-like growth factor I (IGF-I) up-regulate IGF-I gene expression in organ culture of newt testis, Molecular Reproduction and Development, 60(1), 56-64, 20010401
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Publications such as books

  1. 2013, 標的遺伝子の改変を可能にする人工ヌクレアーゼの開発、化学工業, 2013
  2. 2012/07, 人工ヌクレアーゼを用いたゲノム編集, 2012, 07
  3. 2006/04, DNA Structure, Chromatin and Gene ExpressionChapter 14, "Involvement of nuclear matrix and chromatin loop formation in the function of insulators.", 2006, 04, 8178952289
  4. 2006/04, Involvement of nuclear matrix and chromatin loop formation in the function of insulators (DNA structure, chromatin and gene expression) , 2006, 04, 8178952289, 252
  5. 2001, Initiation of meiosis in organ culture of newt testes by IGF-I , Perspective in Comparative Endocrinolog, 2001
  6. 1998, 両生類の発生生物学 , 1998
  7. 1988/08, 両生類における精子分化機構. 「生殖細胞の発生と性分化」, 1988, 08
  8. 1997, Proliferation of newt spermatogonia in vitro and expression of activin β subunit genes by Sertoli Cells. , J. Reprod. Dev., 1997
  9. 1996, Expression of activin beta subunit and type II receptor genes during newt spermatogenesis. , Inhibin= Activin and Follistatin, 1996

Awards

  1. 2016年11月14日, Blue Flame Award, (NPO) Addgene
  2. 2013年09月11日, JB Award, 公益社団法人日本生化学会会長, Nucleosome exclusion from the interspecies conserved central AT-rich region of the Ars insulator.
  3. 1999年09月, Zoological Science Award, The Zoological Society of Japan, Cloning and expression of a cDNA encoding a prolactin receptor of the Japanese red-bellied newt, Cynops pyrrhogaster

Works, etc.

  1. 2016
  2. 2016
  3. 2015
  4. 2015
  5. 2015
  6. 2015
  7. 2015
  8. 2014
  9. ・「数理生命科学ディレクター養成プログラム」取組報告書, 2007/04

External Funds

Acceptance Results of Competitive Funds

  1. 2014, 2018
  2. 2014, 2018
  3. Practical Research Project for Rare / Intractable Diseases, 2015/11/16, 2016/03/31
  4. KAKENHI, 2014, 2016
  5. 2015
  6. 2014, 2017
  7. Health and Labour Sciences Research Grants, 2014/06/02, 2015/03/31
  8. 2013, 2015
  9. 2013
  10. 2012
  11. 2011
  12. 2008/04, 2009/03
  13. KAKENHI, Quantitative analysis of cell-cell interaction responsible for the robustness of development, 2009, 2011
  14. KAKENHI, Gene expression noise during cell differentiation and its regulation, 2008, 2010
  15. KAKENHI, 2008, 2009
  16. KAKENHI, Studies on the gene network responsible for the specific gene expression during development, 2005, 2007
  17. 2005
  18. KAKENHI, 2004, 2005
  19. KAKENHI, 2002, 2003
  20. KAKENHI, Switching Mechanism for Meiosis Initiation or Apoptosis in Spermatogonia, 2000, 2001
  21. KAKENHI, 2000, 2001
  22. 2000
  23. KAKENHI, 1998, 1999
  24. KAKENHI, Mechanism of apoptosis induction in amphibian spermatogonia by prolactin, 1997, 1999
  25. KAKENHI, 1996, 1996
  26. KAKENHI, 1995, 1995
  27. KAKENHI, 1995, 1995
  28. KAKENHI, Analysis of the mechanism underlying meiosis initiation using in vitro culture system., 1993, 1995
  29. KAKENHI, 2013, 2016
  30. KAKENHI, 2013, 2015

Social Activities

Organizing Academic Conferences, etc.

  1. 2018/02
  2. 2015/11, 2015/11
  3. 2014/10
  4. International Symposium on RNAi and Genome Editing Methods, 2014/03, 2014/03
  5. 2013/10
  6. 2012/10
  7. 2012/02
  8. 2011/, 2014/
  9. 2011/
  10. 2006/06
  11. 1998/05

History as Peer Reviews of Academic Papers

  1. 2016, Nature Communications