Takashi Yamamoto

Last Updated :2019/05/07

Affiliations, Positions
Graduate School of Integrated Sciences for Life, ., Professor
E-mail
tybighiroshima-u.ac.jp
Other Contact Details
1-3-1,Kagamiyama,Higashi-Hiroshima, Japan
TEL : (+81)824-24-7446 FAX : (+81)824-24-7498

Basic Information

Major Professional Backgrounds

  • 1992/08/01, 2002/05/31, Kumamoto University, Research Associate
  • 2002/06/01, 2003/10/31, Hiroshima University, Graduate School of Science , Lecturer
  • 2003/11/01, 2004/03/31, Hiroshima University, Graduate School of Science , Assistant Professor
  • 2004/04/01, Hiroshima University, Graduate School of Science , Professor
  • 2014/04/01, Tottori University, Visiting professor
  • 2014/04/01, Kumamoto University, Visiting professor
  • 2017/04/01, Collaborative Research Laboratory for Next Generation Automobile Technology, Professor
  • 2019/02/01, Genome Editing Innovation Center, Director of the center

Educational Backgrounds

  • Hiroshima University, Graduate School, Division of Natural Science, Japan, 1991/04, 1992/07
  • Hiroshima University, Graduate School, Division of Natural Science, Japan, 1989/04, 1991/03
  • Hiroshima University, Faculty of Science, Japan, 1985/04, 1989/03

Academic Degrees

  • Doctor of Science, Hiroshima University
  • Master of Science, Hiroshima University

Educational Activity

  • School of Science:Biological Sciences, Graduate School of Science:Mathematical and Life Sciences, Graduate School of Science:Mathematical and Life Sciences

Research Fields

  • Biological Sciences;Genome science;Genome biology
  • Biological Sciences;Genome science;System genome science
  • Biology;Biological Science;Developmental biology

Research Keywords

  • Genome Editing
  • TALEN, CRISPR-Cas, ZFN
  • echinoderm
  • development
  • noise
  • gene regulatory network

Affiliated Academic Societies

  • The Japanese Society for Genome Editing, 2016

Educational Activity

Course in Charge

  1. 2019, Liberal Arts Education Program1, 3Term, Introduction to Biology
  2. 2019, Undergraduate Education, 4Term, Introduction to Biological Sciences B
  3. 2019, Undergraduate Education, 2Term, Advanced Biology
  4. 2019, Undergraduate Education, 3Term, Developmental Biology A
  5. 2019, Undergraduate Education, Second Semester, Seminar for Molecular Genetics
  6. 2019, Undergraduate Education, First Semester, Special Study for Graduation
  7. 2019, Undergraduate Education, Second Semester, Special Study for Graduation
  8. 2019, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
  9. 2019, Graduate Education (Master's Program) , First Semester, Introduction to Life Sciences
  10. 2019, Graduate Education (Master's Program) , First Semester, Special Research for Mathematical and Life Sciences
  11. 2019, Graduate Education (Master's Program) , Second Semester, Special Research for Mathematical and Life Sciences
  12. 2019, Graduate Education (Doctoral Program) , First Semester, Special Research for Mathematical and Life Sciences
  13. 2019, Graduate Education (Doctoral Program) , Second Semester, Special Research for Mathematical and Life Sciences
  14. 2019, Graduate Education (Doctoral Program) , First Semester, Seminar of Molecular Genetics
  15. 2019, Graduate Education (Doctoral Program) , Second Semester, Seminar of Molecular Genetics
  16. 2019, Graduate Education (Master's Program) , 2Term, Topical Seminar in Life Science A
  17. 2019, Graduate Education (Master's Program) , 4Term, Topical Seminar in Life Science B
  18. 2019, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences
  19. 2019, Graduate Education (Master's Program) , 1Term, Introduction to Life Science
  20. 2019, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Mathematical and Life Sciences
  21. 2019, Graduate Education (Master's Program) , 1Term, Exercises in Life Science A
  22. 2019, Graduate Education (Master's Program) , 2Term, Exercises in Life Science A
  23. 2019, Graduate Education (Master's Program) , 3Term, Exercises in Life Science B
  24. 2019, Graduate Education (Master's Program) , 4Term, Exercises in Life Science B
  25. 2019, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar A
  26. 2019, Graduate Education (Master's Program) , 1Term, Advanced Technologies for Life Science
  27. 2019, Graduate Education (Master's Program) , 1Term, Exercises in Biomedical Science A
  28. 2019, Graduate Education (Master's Program) , 2Term, Exercises in Biomedical Science A
  29. 2019, Graduate Education (Master's Program) , 3Term, Exercises in Biomedical Science B
  30. 2019, Graduate Education (Master's Program) , 4Term, Exercises in Biomedical Science B
  31. 2019, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biomedial Science
  32. 2019, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar C
  33. 2019, Graduate Education (Master's Program) , OutOfTerm(1st), Ethics of Genome Editing Research
  34. 2019, Graduate Education (Master's Program) , 2Term, Basics and Practical Applications of Genome Editing
  35. 2019, Graduate Education (Master's Program) , OutOfTerm(1st), Basic Training of Genome Editing

Research Activities

Academic Papers

  1. Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9, NATURE COMMUNICATIONS, 5, 201411
  2. Biased genome editing using the local accumulation of DSB repair molecules system, NATURE COMMUNICATIONS, 9, AUG 16 2018
  3. Efficient TALEN construction and evaluation methods for human cell and animal applications, GENES TO CELLS, 18(4), 315-326, 201304
  4. Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity, SCIENTIFIC REPORTS, 3, 20131129
  5. Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(27), 10915-10920, 20120703
  6. Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system, SCIENTIFIC REPORTS, 4, 20140623
  7. Microhomology-assisted scarless genome editing in human iPSCs, NATURE COMMUNICATIONS, 9, 20180305
  8. Identification of a cell-penetrating peptide applicable to a protein-based transcription activator-like effector expression system for cell engineering, BIOMATERIALS, 173, 11-21, AUG 2018
  9. MET Activation by a Macrocyclic Peptide Agonist that Couples to Biological Responses Differently from HGF in a Context-Dependent Manner, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 19(10), 201810
  10. Exploration of genetic basis underlying individual differences in radiosensitivity within human populations using genome editing technology, JOURNAL OF RADIATION RESEARCH, 59, 201804
  11. Generation of and characterization of anti-IL-11 antibodies using newly established Il11-deficient mice, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 505(2), 453-459, 20181028
  12. Differential micronucleus frequency in isogenic human cells deficient in DNA repair pathways is a valuable indicator for evaluating genotoxic agents and their genotoxic mechanisms, ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 59(6), 529-538, 201807
  13. Replication stress induces accumulation of FANCD2 at central region of large fragile genes, NUCLEIC ACIDS RESEARCH, 46(6), 2932-2944, APR 6 2018
  14. Cas9 ribonucleoprotein complex allows direct and rapid analysis of coding and noncoding regions of target genes in Pleurodeles waltl development and regeneration, DEVELOPMENTAL BIOLOGY, 443(2), 127-136, 20181115
  15. Acceleration of cancer science with genome editing and related technologies, CANCER SCIENCE, 109(12), 3679-3685, 201812
  16. Unexpected heterogeneity derived from Cas9 ribonucleoprotein-introduced clonal cells at the HPRT1 locus, GENES TO CELLS, 23(4), 255-263, 201804
  17. Scleraxis is a transcriptional activator that regulates the expression of Tenomodulin, a marker of mature tenocytes and ligamentocytes, SCIENTIFIC REPORTS, 8, 20180216
  18. Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes, STEM CELL REPORTS, 11(3), 727-740, SEP 11 2018
  19. Functional analysis of thyroid hormone receptor beta in Xenopus tropicalis founders using CRISPR-Cas, BIOLOGY OPEN, 7(1), 201801
  20. HpBase: A genome database of a sea urchin, Hemicentrotus pulcherrimus, DEVELOPMENT GROWTH & DIFFERENTIATION, 60(3), 174-182, 201804
  21. Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 125(5), 599-605, 201805
  22. Cancer induction and suppression with transcriptional control and epigenome editing technologies, JOURNAL OF HUMAN GENETICS, 63(2), 187-194, 201802
  23. Cas9, Cpf1 and C2c1/2/3-What's next?, BIOENGINEERED, 8(3), 265-273, 2017
  24. Establishment of expanded and streamlined pipeline of PITCh knock-in - a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO, BIOENGINEERED, 8(3), 302-308, 2017
  25. Highly efficient biallelic genome editing of human ES/iPS cells using a CRISPR/Cas9 or TALEN system, NUCLEIC ACIDS RESEARCH, 45(9), 5198-5207, 20170519
  26. Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice, BIOLOGY OPEN, 6(5), 706-713, 20170515
  27. PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells, SCIENTIFIC REPORTS, 7, 20170703
  28. Germ cell regeneration-mediated, enhanced mutagenesis in the ascidian Ciona intestinalis reveals flexible germ cell formation from different somatic cells, DEVELOPMENTAL BIOLOGY, 423(2), 111-125, 20170315
  29. Participation of androgen and its receptor in sex determination of an amphibian species, PLOS ONE, 12(6), 20170605
  30. TALEN-mediated targeted editing of the GDE5 gene suppresses fibroblastic cell proliferation, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81(11), 2164-2167, 2017
  31. Hox-mediated endodermal identity patterns pharyngeal muscle formation in the chordate pharynx, DEVELOPMENT, 144(9), 1629-1634, 20170501
  32. Clustered Xenopus keratin genes: A genomic, transcriptomic, and proteomic analysis, DEVELOPMENTAL BIOLOGY, 426(2), 384-392, 20170615
  33. Developmental changes in drug-metabolizing enzyme expression during metamorphosis of Xenopus tropicalis, JOURNAL OF TOXICOLOGICAL SCIENCES, 42(5), 605-613, 201710
  34. Establishment of pten knockout medaka with transcription activator-like effector nucleases (TALENs) as a model of PTEN deficiency disease, PLOS ONE, 12(10), 20171020
  35. Evaluation of ATM heterozygous mutations underlying individual differences in radiosensitivity using genome editing in human cultured cells, SCIENTIFIC REPORTS, 7, 20170720
  36. Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123(3), 287-293, 201703
  37. Modification of single-nucleotide polymorphism in a fully humanized CYP3A mouse by genome editing technology, SCIENTIFIC REPORTS, 7, 20171109
  38. MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems, Nature Protocols, 11, 118-133, 2016
  39. Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ, BMC GENOMICS, 17, 20161128
  40. Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice, BIOLOGY OPEN, 5(8), 1142-1148, 20160815
  41. Establishment of Functional Genomics Pipeline in Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9, HUMAN GENE THERAPY, 27(6), 436-450, 201606
  42. In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration, GENES TO CELLS, 21(4), 358-369, 201604
  43. C-Type Lectin Receptor DCAR Recognizes Mycobacterial Phosphatidyl-Inositol Mannosides to Promote a Th1 Response during Infection, IMMUNITY, 45(6), 1245-1257, 20161220
  44. Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation, JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 27(6), 1778-1791, 201606
  45. Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets, MOLECULAR REPRODUCTION AND DEVELOPMENT, 83(1), 61-70, 201601
  46. ALC1/CHD1L, a chromatin-remodeling enzyme, is required for efficient base excision repair, PLOS ONE, 12(11), 20171117
  47. Highly multiplexed CRISPR-Cas9-nuclease and Cas9-nickase vectors for inactivation of hepatitis B virus, GENES TO CELLS, 21(11), 1253-1262, 201611
  48. Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation, PLOS GENETICS, 12(1), 201601
  49. Involvement of aspartoacylase in tremor expression in rats, EXPERIMENTAL ANIMALS, 65(3), 293-301, 201607
  50. Systematic Cellular Disease Models Reveal Synergistic Interaction of Trisomy 21 and GATA1 Mutations in Hematopoietic Abnormalities, CELL REPORTS, 15(6), 1228-1241, 20160510
  51. Rapid and efficient analysis of gene function using CRISPR-Cas9 in Xenopus tropicalis founders, GENES TO CELLS, 21(7), 755-771, 201607
  52. Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing, CELL STEM CELL, 19(1), 127-138, 20160707
  53. Depdc5 knockout rat: A novel model of mTORopathy, NEUROBIOLOGY OF DISEASE, 89, 180-189, 201605
  54. Cilia play a role in breaking left-right symmetry of the sea urchin embryo, GENES TO CELLS, 21(6), 568-578, 201606
  55. Single-Cell-State Culture of Human Pluripotent Stem Cells Increases Transfection Efficiency, BIORESEARCH OPEN ACCESS, 5(1), 127-136, 201605
  56. Quadruple zebrafish mutant reveals different roles of Mesp genes in somite segmentation between mouse and zebrafish, DEVELOPMENT, 143(15), 2842-2852, 201608
  57. Transcriptional regulation of a horizontally transferred gene from bacterium to chordate, PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 283(1845), 20161228
  58. Functional consequence of fibulin-4 missense mutations associated with vascular and skeletal abnormalities and cutis laxa, MATRIX BIOLOGY, 56, 132-149, 201612
  59. Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells, STEM CELL REPORTS, 6(4), 496-510, 20160412
  60. Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish, GENES TO CELLS, 21(5), 492-504, 201605
  61. Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy, SCIENTIFIC REPORTS, 6, 20161124
  62. Report on the Conference on Transposition and Genome Engineering 2015 (TGE 2015): advancing cutting-edge genomics technology in the ancient city of Nara, GENES TO CELLS, 21(5), 392-395, 201605
  63. Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice, GENOME BIOLOGY, 16, 20150429
  64. The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos, PLOS ONE, 10(11), 20151118
  65. Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells, CELL STEM CELL, 17(2), 178-194, 20150806
  66. Simultaneous live imaging of the transcription and nuclear position of specific genes, NUCLEIC ACIDS RESEARCH, 43(19), 20151030
  67. Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 16(10), 23849-23866, 201510
  68. Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9, SCIENTIFIC REPORTS, 5, 20150609
  69. Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes, BMC BIOTECHNOLOGY, 15, 20150522
  70. Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish, SCIENTIFIC REPORTS, 5, 20150305
  71. Smarcal1 promotes double-strand-break repair by nonhomologous end-joining, NUCLEIC ACIDS RESEARCH, 43(13), 6359-6372, 20150727
  72. Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9, STEM CELL REPORTS, 5(1), 75-82, 20150714
  73. The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation, CELL REPORTS, 10(5), 664-673, 20150210
  74. Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus, BIOTECHNOLOGY AND BIOENGINEERING, 112(7), 1335-1342, 201507
  75. Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway, JOURNAL OF CELL BIOLOGY, 211(4), 775-784, 20151123
  76. Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine, DEVELOPMENTAL BIOLOGY, 403(1), 43-56, 20150701
  77. A High Excision Potential of TALENs for Integrated DNA of HIV-Based Lentiviral Vector, PLOS ONE, 10(3), 20150317
  78. Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs, IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 51(9), 879-884, 201510
  79. Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9, STEM CELL REPORTS, 4(1), 143-154, 20150113
  80. Unliganded Thyroid Hormone Receptor alpha Regulates Developmental Timing via Gene Repression in Xenopus tropicalis, ENDOCRINOLOGY, 156(2), 735-744, 201502
  81. Epithelial DLD-1 Cells with Disrupted E-cadherin Gene Retain the Ability to Form Cell Junctions and Apico-basal Polarity, CELL STRUCTURE AND FUNCTION, 40(2), 79-94, 2015
  82. Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring the juxtamembrane region and plakophilins, JOURNAL OF BIOCHEMISTRY, 158(4), 339-353, 201510
  83. Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines, GENES TO CELLS, 20(12), 1059-1076, 201512
  84. Targeted gene correction of RUNX1 in induced pluripotent stem cells derived from familial platelet disorder with propensity to myeloid malignancy restores normal megakaryopoiesis, EXPERIMENTAL HEMATOLOGY, 43(10), 849-857, 201510
  85. TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS ( MVA) syndrome, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(4), 1461-1466, 20140128
  86. EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step, JOURNAL OF CELL BIOLOGY, 206(3), 347-356, 20140804
  87. FAST-id system for enrichment of cells with TALEN-induced mutations and large deletions, GENES TO CELLS, 19(5), 419-431, 201405
  88. Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells, SCIENTIFIC REPORTS, 4, 20141120
  89. Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems, SCIENTIFIC REPORTS, 4, 20140716
  90. Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing, EXPERIMENTAL ANIMALS, 63(3), 349-355, 201407
  91. Simple knockout by electroporation of engineered endonucleases into intact rat embryos, SCIENTIFIC REPORTS, 4, 20141001
  92. Sterol Side Chain Reductase 2 Is a Key Enzyme in the Biosynthesis of Cholesterol, the Common Precursor of Toxic Steroidal Glycoalkaloids in Potato, PLANT CELL, 26(9), 3763-3774, 201409
  93. TALEN-induced gene knock out in Drosophila, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 86-91, 201401
  94. Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies, SCIENTIFIC REPORTS, 4, 20140827
  95. Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 108-114, 201401
  96. Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 115-121, 201401
  97. Screening Methods to Identify TALEN-Mediated Knockout Mice, EXPERIMENTAL ANIMALS, 63(1), 79-84, 201401
  98. Versatile strategy for isolating transcription activator-like effector nuclease-mediated knockout mutants in Caenorhabditis elegans, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 78-85, 201401
  99. Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex, BMC BIOTECHNOLOGY, 14, 20141118
  100. Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona, DEVELOPMENT, 141(2), 481-487, 20140115
  101. Targeted mutagenesis in sea urchin embryos using TALENs, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 92-97, 201401
  102. Germ Cell Mutations of the Ascidian Ciona intestinalis With TALE Nucleases, GENESIS, 52(5), 431-439, 201405
  103. Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes, SCIENTIFIC REPORTS, 3, 20130213
  104. Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome, DEVELOPMENTAL BIOLOGY, 375(1), 79-91, 20130301
  105. Efficient Targeted Mutagenesis in Medaka Using Custom-Designed Transcription Activator-Like Effector Nucleases, GENETICS, 193(3), 739-+, 201303
  106. High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos., Biol Open, 2, 448-452, 20130301
  107. Production of Sry knockout mouse using TALEN via oocyte injection, SCIENTIFIC REPORTS, 3, 20131105
  108. Quantitative assay for TALEN activity at endogenous genomic loci, BIOLOGY OPEN, 2(4), 363-367, 20130415
  109. The 3 ' UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin, DEVELOPMENTAL BIOLOGY, 377(1), 275-283, 20130501
  110. Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 54(5), 535-545, 201206
  111. Targeted disruption of exogenous EGFP gene in medaka using zinc-finger nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 54(5), 546-556, 201206
  112. Equarin is involved as an FGF signaling modulator in chick lens differentiation, DEVELOPMENTAL BIOLOGY, 368(1), 109-117, 20120801
  113. Nucleosome exclusion from the interspecies-conserved central AT-rich region of the Ars insulator, JOURNAL OF BIOCHEMISTRY, 151(1), 75-87, 201201
  114. Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases, NATURE COMMUNICATIONS, 3, 201208
  115. HpSumf1 is involved in the activation of sulfatases responsible for regulation of skeletogenesis during sea urchin development, DEVELOPMENT GENES AND EVOLUTION, 221(3), 157-166, 201108
  116. Implication of HpEts in Gene Regulatory Networks Responsible for Specification of Sea Urchin Skeletogenic Primary Mesenchyme Cells, ZOOLOGICAL SCIENCE, 27(8), 638-646, 201008
  117. Targeted mutagenesis in the sea urchin embryo using zinc-finger nucleases, GENES TO CELLS, 15(8), 875-885, 201008
  118. Dicer is Required for the Normal Development of Sea Urchin, Hemicentrotus pulcherrimus, ZOOLOGICAL SCIENCE, 27(6), 477-486, 201006
  119. HpSulf, a heparan sulfate 6-O-endosulfatase, is involved in the regulation of VEGF signaling during sea urchin development, MECHANISMS OF DEVELOPMENT, 127(3-4), 235-245, 2010
  120. Mammalian arylsulfatase A functions as a novel component of the extracellular matrix, CONNECTIVE TISSUE RESEARCH, 51(5), 388-396, 201010
  121. Suppressor of Hairless (Su(H)) is Required for Foregut Development in the Sea Urchin Embryo, ZOOLOGICAL SCIENCE, 26(10), 686-690, 200910
  122. Role of the Nanos Homolog During Sea Urchin Development, DEVELOPMENTAL DYNAMICS, 238(10), 2511-2521, 200910
  123. Analysis of cis-regulatory elements controlling spatio-temporal expression of T-brain gene in sea urchin, Hemicentrotus pulcherrimus, MECHANISMS OF DEVELOPMENT, 125(1-2), 2-17, 2008
  124. RSF governs silent chromatin formation via histone H2Av replacement, PLOS GENETICS, 4(2), 200802
  125. The Ars insulator facilitates I-SceI meganuclease-mediated transgenesis in the sea urchin embryo, DEVELOPMENTAL DYNAMICS, 237(9), 2475-2482, 200809
  126. DNA variations within the sea urchin Otx gene enhancer, FEBS LETTERS, 581(27), 5234-5240, 20071113
  127. Unichrom, a novel nuclear matrix protein, binds to the Ars insulator and canonical MARs, ZOOLOGICAL SCIENCE, 23(1), 9-21, 200601
  128. Developmental expression of HpNanos, the Hemicentrotus pulcherrimus homologue of nanos, GENE EXPRESSION PATTERNS, 6(5), 572-577, 200606
  129. Organ-specific and age-dependent expression of insulin-like growth factor-I (IGF-I) mRNA variants: IGF-IA and IB mRNAs in the mouse, ZOOLOGICAL SCIENCE, 22(9), 1011-1021, 200509
  130. The Otx binding site is required for the activation of HpOtxL mRNA expression in the sea urchin, Hemicentrotus pulcherrimus, Development= Growth and Differentiation, 46(1), 20040201
  131. Sexually dimorphic expression of a teleost homologue of Mullerian inhibiting substance during gonadal sex differentiation in Japanese flounder, Paralichthys olivaceus, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 322(2), 508-513, 20040917
  132. Identification of two teleost homologs of the Drosophila sex determination factor, transformer-2 in medaka (Oryzias latipes), MECHANISMS OF DEVELOPMENT, 121(7-8), 991-996, 200407
  133. The Otx binding site is required for the activation of HpOtxL mRNA expression in the sea urchin, Hemicentrotus pulcherrimus, DEVELOPMENT GROWTH & DIFFERENTIATION, 46(1), 61-69, 200402
  134. A new G-stretch-DNA-binding protein, Unichrom, displays cell-cycle-dependent expression in sea urchin embryos, DEVELOPMENT GROWTH & DIFFERENTIATION, 46(4), 335-341, 200408
  135. Utilization of a particle gun DNA introduction system for the analysis of cis-regulatory elements controlling the spatial expression pattern of the arylsulfatase gene (HpArs) in sea urchin embryos, DEVELOPMENT GENES AND EVOLUTION, 213(1), 44-49, 200302
  136. Abnormal spermatogenesis at low temperatures in the Japanese red-bellied newt, Cynops pyrrhogaster: Possible biological significance of the cessation of Spermatocytogenesis, MOLECULAR REPRODUCTION AND DEVELOPMENT, 66(1), 60-66, 200309
  137. Low temperature promotes annexin V expression in newt testis, ZOOLOGICAL SCIENCE, 20(6), 733-735, 200306
  138. T-brain homologue (HpTb) is involved in the archenteron induction signals of micromere descendant cells in the sea urchin embryo, DEVELOPMENT, 129(22), 5205-5216, 200211
  139. Follicle-stimulating hormone is indispensable for the last spermatogonial mitosis preceding meiosis initiation in newts(Cynops pyrrhogaster), Biology of Reproduction, 66(1), 14-20, 20020401
  140. Promotion of cathepsin L activity in newt spermatogonial apopotosis induced by prolactin, FEBS Letters, 521(1-3), 43-46, 20020401
  141. Human recombinant stem cell factor promotes spermatogonial proliferation, but not meiosis initiation in organ culture of newt testis fragments, Biochemical and Biophysical Research Communications, 294(3), 695-699, 20020401
  142. Caspase activity in newt spermatogonial apoptosis induced by prolactin and cycloheximide., Molecular Reproduction and Development, 52(2), 209-214, 20010401
  143. Expression of a novel matrix metalloproteinase gene during Cynops early embryogenesis, Biochemical and Biophysical Research Communications, 288(2), 380-384, 20010401
  144. Cold Suppression of follicle-stimulating hormone activity on proliferation and survival of newt spermatogonia, General and Comparative Endocrinology, 122(3), 296-303, 20010401
  145. Mammalian follicle-stimulating hormone (FSH) and insulin-like growth factor I (IGF-I) up-regulate IGF-I gene expression in organ culture of newt testis, Molecular Reproduction and Development, 60(1), 56-64, 20010401
  146. Cloning of dynein Intermediate chain cDNA of the newt Cynops pyrrhogaster, DNA Research, 8(2), 81-84, 20010401
  147. Conversion from mitosis to meiosis-morphology and expression of PCNA and Dmc1 during newt spermatogenesis, Development= Growth and Differentiation, 42(2), 603-612, 20000401
  148. Prolactin induces apotosis in the penultimate spermatogonial stage of the testes in Japanese red-bellied Newt (Cynops pyrrhogaster), Endocrinology, 141(6), 2027-2032, 20000401
  149. Molecular cloning, functional characterization and gene expression of a follicle-stimulating hormone receptor in the testis of newt, Cynops pyrrhogaster, Biochemical and Biophysical Research Communications, 275(1), 121-128, 20000401
  150. Cloning of cDNA for Xenopus prolactin receptor and its metamorphic expression profile, Development= Growth and Differentiation, 42(2), 167-174, 20000401
  151. IGF-I, IGF-II and insulin promote differentiation of spermatogonia to primary spermatocytes in organ culture of newt testes, International Journal of Developmental Biology, 43(4), 343-348, 19990401
  152. Newt Rad51-Cloning of cDNA and analysis of gene expression during the spermatogenesis, Development= Growth and Differentiation, 41(4), 401-406, 19990401
  153. Cloning of cDNA for newt WT1 and the differential expression during spermatogenesis of the Japanese newt, Cynops pyrrhogaster, Development= Growth and Differentiation, 40(6), 599-608, 19980401
  154. Cloning and expression of a cDNA encoding a prolactin receptor of the Japanese red-bellied newt, Cynops pyrrhogaster, Zoological Science, 15(5), 741-747, 19980401
  155. Genesis of axial fiber of newt sperm-cDNA cloning and expression of a 29 kDa protein, a major component of the axial fiber, during the spermatogenesis, International Journal of Developmental Biology, 40, 1109-1118, 19960401
  156. Expression of activin beta subunit genes in Sertoli cells of newt testes, Biochemical and Biophysical Research Communications, 224, 451-456, 19960401
  157. Differential expression of annexin V during spermatogenesis in Cynops pyrrhogaster, Development Genes and Evolution, 206, 64-71, 19960401
  158. A long polypyrimidine-polypurine sequence in 5’ flanking region of arylsulfatase gene of sea urchin embryo, International Journal of Developmental Biology, 38, 337-344, 19940401
  159. cis-Elements and protein factors related to regulation of transcription of arylsulfatase gene during sea urchin development, Development= Growth and Differentiation, 35(6), 703-710, 19930401
  160. Cis-acting elements for proper ontogenic expression of arylsulfatase gene of sea urchin embryo, Development= Growth and Differentiation, 34(6), 719-729, 19920401

Publications such as books

  1. 2013, 標的遺伝子の改変を可能にする人工ヌクレアーゼの開発、化学工業, 2013
  2. 2012/07, 人工ヌクレアーゼを用いたゲノム編集, 2012, 07
  3. 2006/04, DNA Structure, Chromatin and Gene ExpressionChapter 14, "Involvement of nuclear matrix and chromatin loop formation in the function of insulators.", 2006, 04, 8178952289
  4. 2006/04, Involvement of nuclear matrix and chromatin loop formation in the function of insulators (DNA structure, chromatin and gene expression) , 2006, 04, 8178952289, 252
  5. 2001, Initiation of meiosis in organ culture of newt testes by IGF-I , Perspective in Comparative Endocrinolog, 2001
  6. 1998, 両生類の発生生物学 , 1998
  7. 1988/08, 両生類における精子分化機構. 「生殖細胞の発生と性分化」, 1988, 08
  8. 1997, Proliferation of newt spermatogonia in vitro and expression of activin β subunit genes by Sertoli Cells. , J. Reprod. Dev., 1997
  9. 1996, Expression of activin beta subunit and type II receptor genes during newt spermatogenesis. , Inhibin= Activin and Follistatin, 1996

Awards

  1. 2016年11月14日, Blue Flame Award, (NPO) Addgene
  2. 2013年09月11日, JB Award, 公益社団法人日本生化学会会長, Nucleosome exclusion from the interspecies conserved central AT-rich region of the Ars insulator.
  3. 1999年09月, Zoological Science Award, The Zoological Society of Japan, Cloning and expression of a cDNA encoding a prolactin receptor of the Japanese red-bellied newt, Cynops pyrrhogaster

Works, etc.

  1. 2017
  2. 2016
  3. 2016
  4. 2015
  5. 2015
  6. 2015
  7. 2015
  8. 2015
  9. 2014
  10. ・DNA結合ドメインを含むポリペプチド(特願2013-166768), 2013/08
  11. ・核酸挿入用ベクター(特願2013-230349), 2013/09
  12. ・PPRモチーフを利用したDNA結合性蛋白質の設計方法及びその利用(特願2013-88940), 2013/04
  13. ・ヌクレアーゼを発現させるためのベクターおよびベクターセット、ならびに変異導入を受けた細胞の取得方法(特願2013-098724), 2013/05
  14. ・新規DNA結合ドメインおよびそれを含む新規DNA切断酵素(特願2011-242250), 2011/11
  15. ・「数理生命科学ディレクター養成プログラム」取組報告書, 2007/04

External Funds

Acceptance Results of Competitive Funds

  1. Practical Research Project for Rare / Intractable Diseases, 2015/11/16, 2016/03/31
  2. KAKENHI, 2014, 2016
  3. 2014
  4. 2015
  5. 2014, 2017
  6. Health and Labour Sciences Research Grants, 2014/06/02, 2015/03/31
  7. 2013, 2015
  8. 2013
  9. 2012
  10. 2011
  11. 2008/04, 2009/03
  12. KAKENHI, Quantitative analysis of cell-cell interaction responsible for the robustness of development, 2009, 2011
  13. KAKENHI, Gene expression noise during cell differentiation and its regulation, 2008, 2010
  14. KAKENHI, 2008, 2009
  15. KAKENHI, Studies on the gene network responsible for the specific gene expression during development, 2005, 2007
  16. 2005
  17. KAKENHI, 2004, 2005
  18. KAKENHI, 2002, 2003
  19. KAKENHI, Switching Mechanism for Meiosis Initiation or Apoptosis in Spermatogonia, 2000, 2001
  20. KAKENHI, 2000, 2001
  21. 2000
  22. KAKENHI, 1998, 1999
  23. KAKENHI, Mechanism of apoptosis induction in amphibian spermatogonia by prolactin, 1997, 1999
  24. KAKENHI, 1996, 1996
  25. KAKENHI, 1995, 1995
  26. KAKENHI, 1995, 1995
  27. KAKENHI, Analysis of the mechanism underlying meiosis initiation using in vitro culture system., 1993, 1995
  28. KAKENHI, 2013, 2016
  29. KAKENHI, 2013, 2015

Social Activities

Organizing Academic Conferences, etc.

  1. 2018/02
  2. 2015/11, 2015/11
  3. 2014/10
  4. International Symposium on RNAi and Genome Editing Methods, 2014/03, 2014/03
  5. 2013/10
  6. 2012/10
  7. 2012/02
  8. 2011/, 2014/
  9. 2011/
  10. 2006/06
  11. 1998/05

History as Peer Reviews of Academic Papers

  1. 2016, Nature Communications