Takashi Yamamoto

Last Updated :2017/09/01

Affiliations, Positions
Graduate School of Science, ., Professor
E-mail
tybighiroshima-u.ac.jp
Other Contact Details
1-3-1,Kagamiyama,Higashi-Hiroshima, Japan
TEL : (+81)824-24-7446 FAX : (+81)824-24-7498

Basic Information

Major Professional Backgrounds

  • 1992/08/01, 2002/05/31, Kumamoto University, Research Associate
  • 2002/06/01, 2003/10/31, Hiroshima University, Graduate School of Science , Lecturer
  • 2003/11/01, 2004/03/31, Hiroshima University, Graduate School of Science , Assistant Professor
  • 2004/04/01, Hiroshima University, Graduate School of Science , Professor
  • 2014/04/01, Tottori University, Visiting professor
  • 2014/04/01, Kumamoto University, Visiting professor
  • 2017/04/01, Collaborative Research Laboratory for Next Generation Automobile Technology, Professor

Educational Backgrounds

  • Hiroshima University, Graduate School, Division of Natural Science, Japan, 1991/04, 1992/07
  • Hiroshima University, Graduate School, Division of Natural Science, Japan, 1989/04, 1991/03
  • Hiroshima University, Faculty of Science, Japan, 1985/04, 1989/03

Academic Degrees

  • Doctor of Science, Hiroshima University
  • Master of Science, Hiroshima University

Educational Activity

  • School of Science:Biological Sciences, Graduate School of Science:Mathematical and Life Sciences, Graduate School of Science:Mathematical and Life Sciences

Research Fields

  • Biological Sciences;Genome science;Genome biology
  • Biological Sciences;Genome science;System genome science
  • Biology;Biological Science;Developmental biology

Research Keywords

  • Genome Editing
  • TALEN, CRISPR-Cas, ZFN
  • echinoderm
  • development
  • noise
  • gene regulatory network

Affiliated Academic Societies

  • The Japanese Society for Genome Editing, 2016

Educational Activity

Course in Charge

  1. 2017, Liberal Arts Education Program1, Second Semester, Introduction to Biological Sciences B
  2. 2017, Undergraduate Education, First Semester, Systembiology
  3. 2017, Undergraduate Education, First Semester, Advanced Biology
  4. 2017, Undergraduate Education, First Semester, Basic Biological Science A
  5. 2017, Undergraduate Education, Second Semester, Developmental biology A
  6. 2017, Undergraduate Education, Second Semester, Seminar for molecular genetics
  7. 2017, Undergraduate Education, First Semester, Special Study for Graduation
  8. 2017, Undergraduate Education, Second Semester, Special Study for Graduation
  9. 2017, Undergraduate Education, First Semester, Recent Developments in Biological Sciences
  10. 2017, Graduate Education (Master's Program) , Second Semester, Genomics
  11. 2017, Graduate Education (Master's Program) , First Semester, Introduction to Life Sciences
  12. 2017, Graduate Education (Master's Program) , First Semester, Special Research for Mathematical and Life Sciences
  13. 2017, Graduate Education (Master's Program) , Second Semester, Special Research for Mathematical and Life Sciences
  14. 2017, Graduate Education (Master's Program) , First Semester, Seminar of Molecular Genetics
  15. 2017, Graduate Education (Master's Program) , Second Semester, Seminar of Molecular Genetics
  16. 2017, Graduate Education (Doctoral Program) , First Semester, Special Research for Mathematical and Life Sciences
  17. 2017, Graduate Education (Doctoral Program) , Second Semester, Special Research for Mathematical and Life Sciences
  18. 2017, Graduate Education (Doctoral Program) , First Semester, Seminar of Molecular Genetics
  19. 2017, Graduate Education (Doctoral Program) , Second Semester, Seminar of Molecular Genetics

Research Activities

Academic Papers

  1. Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9, NATURE COMMUNICATIONS, 5, NOV 2014
  2. Efficient TALEN construction and evaluation methods for human cell and animal applications, GENES TO CELLS, 18(4), 315-326, APR 2013
  3. Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity, SCIENTIFIC REPORTS, 3, NOV 29 2013
  4. Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(27), 10915-10920, JUL 3 2012
  5. Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system, SCIENTIFIC REPORTS, 4, JUN 23 2014
  6. Cas9, Cpf1 and C2c1/2/3-What's next?, BIOENGINEERED, 8(3), 265-273, 2017
  7. Establishment of expanded and streamlined pipeline of PITCh knock-in - a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO, BIOENGINEERED, 8(3), 302-308, 2017
  8. Highly efficient biallelic genome editing of human ES/iPS cells using a CRISPR/Cas9 or TALEN system, NUCLEIC ACIDS RESEARCH, 45(9), 5198-5207, MAY 19 2017
  9. Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice, BIOLOGY OPEN, 6(5), 706-713, MAY 15 2017
  10. PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells, SCIENTIFIC REPORTS, 7, JUL 3 2017
  11. Germ cell regeneration-mediated, enhanced mutagenesis in the ascidian Ciona intestinalis reveals flexible germ cell formation from different somatic cells, DEVELOPMENTAL BIOLOGY, 423(2), 111-125, MAR 15 2017
  12. Participation of androgen and its receptor in sex determination of an amphibian species, PLOS ONE, 12(6), JUN 5 2017
  13. Hox-mediated endodermal identity patterns pharyngeal muscle formation in the chordate pharynx, DEVELOPMENT, 144(9), 1629-1634, MAY 1 2017
  14. MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems, Nature Protocols, 11, 118-133, 2016
  15. Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ, BMC GENOMICS, 17, NOV 28 2016
  16. Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice, BIOLOGY OPEN, 5(8), 1142-1148, AUG 15 2016
  17. Establishment of Functional Genomics Pipeline in Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9, HUMAN GENE THERAPY, 27(6), 436-450, JUN 2016
  18. In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration, GENES TO CELLS, 21(4), 358-369, APR 2016
  19. C-Type Lectin Receptor DCAR Recognizes Mycobacterial Phosphatidyl-Inositol Mannosides to Promote a Th1 Response during Infection, IMMUNITY, 45(6), 1245-1257, DEC 20 2016
  20. Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation, JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 27(6), 1778-1791, JUN 2016
  21. Efficient modification of myostatin gene in porcine somatic cells and generation of knockout piglets, Molecular Reproduction and Development, 83, 61-70, 2016
  22. Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets, MOLECULAR REPRODUCTION AND DEVELOPMENT, 83(1), 61-70, JAN 2016
  23. Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123(3), 287-293, MAR 2017
  24. Highly multiplexed CRISPR-Cas9-nuclease and Cas9-nickase vectors for inactivation of hepatitis B virus, GENES TO CELLS, 21(11), 1253-1262, NOV 2016
  25. Functional investigation of a non-coding variant associated with adolescent idiopathic scoliosis in zebrafish: elevated expression of the ladybird homeobox gene causes body axis deformation, PLoS Genetics, 12, e1005802, 2016
  26. Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation, PLOS GENETICS, 12(1), JAN 2016
  27. Involvement of aspartoacylase in tremor expression in rats, EXPERIMENTAL ANIMALS, 65(3), 293-301, JUL 2016
  28. Systematic Cellular Disease Models Reveal Synergistic Interaction of Trisomy 21 and GATA1 Mutations in Hematopoietic Abnormalities, CELL REPORTS, 15(6), 1228-1241, MAY 10 2016
  29. Rapid and efficient analysis of gene function using CRISPR-Cas9 in Xenopus tropicalis founders, GENES TO CELLS, 21(7), 755-771, JUL 2016
  30. Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing, CELL STEM CELL, 19(1), 127-138, JUL 7 2016
  31. Depdc5 knockout rat: A novel model of mTORopathy, NEUROBIOLOGY OF DISEASE, 89, 180-189, MAY 2016
  32. Cilia play a role in breaking left-right symmetry of the sea urchin embryo, GENES TO CELLS, 21(6), 568-578, JUN 2016
  33. Single-Cell-State Culture of Human Pluripotent Stem Cells Increases Transfection Efficiency, BIORESEARCH OPEN ACCESS, 5(1), 127-136, MAY 2016
  34. Quadruple zebrafish mutant reveals different roles of Mesp genes in somite segmentation between mouse and zebrafish, DEVELOPMENT, 143(15), 2842-2852, AUG 2016
  35. Transcriptional regulation of a horizontally transferred gene from bacterium to chordate, PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 283(1845), DEC 28 2016
  36. Functional consequence of fibulin-4 missense mutations associated with vascular and skeletal abnormalities and cutis laxa, MATRIX BIOLOGY, 56, 132-149, DEC 2016
  37. Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells, STEM CELL REPORTS, 6(4), 496-510, APR 12 2016
  38. Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish, 21(492), 492-504, 2016
  39. Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish, GENES TO CELLS, 21(5), 492-504, MAY 2016
  40. Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy, SCIENTIFIC REPORTS, 6, NOV 24 2016
  41. Report on the Conference on Transposition and Genome Engineering 2015 (TGE 2015): advancing cutting-edge genomics technology in the ancient city of Nara, GENES TO CELLS, 21(5), 392-395, MAY 2016
  42. Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice, GENOME BIOLOGY, 16, APR 29 2015
  43. The Expression of TALEN before Fertilization Provides a Rapid Knock-out Phenotype in Xenopus laevis Founder Embryos, 2015
  44. The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos, PLOS ONE, 10(11), NOV 18 2015
  45. Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells, CELL STEM CELL, 17(2), 178-194, AUG 6 2015
  46. Simultaneous live imaging of the transcription and nuclear position of specific genes, NUCLEIC ACIDS RESEARCH, 43(19), OCT 30 2015
  47. Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 16(10), 23849-23866, OCT 2015
  48. Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9, SCIENTIFIC REPORTS, 5, JUN 9 2015
  49. Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes, BMC BIOTECHNOLOGY, 15, MAY 22 2015
  50. Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish, SCIENTIFIC REPORTS, 5, MAR 5 2015
  51. Smarcal1 promotes double-strand-break repair by nonhomologous end-joining, NUCLEIC ACIDS RESEARCH, 43(13), 6359-6372, JUL 27 2015
  52. Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9, STEM CELL REPORTS, 5(1), 75-82, JUL 14 2015
  53. The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation, CELL REPORTS, 10(5), 664-673, FEB 10 2015
  54. Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus, BIOTECHNOLOGY AND BIOENGINEERING, 112(7), 1335-1342, JUL 2015
  55. Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway, JOURNAL OF CELL BIOLOGY, 211(4), 775-784, NOV 23 2015
  56. Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine, DEVELOPMENTAL BIOLOGY, 403(1), 43-56, JUL 1 2015
  57. A High Excision Potential of TALENs for Integrated DNA of HIV-Based Lentiviral Vector, PLOS ONE, 10(3), MAR 17 2015
  58. Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs, IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 51(9), 879-884, OCT 2015
  59. Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9, STEM CELL REPORTS, 4(1), 143-154, JAN 13 2015
  60. Unliganded Thyroid Hormone Receptor alpha Regulates Developmental Timing via Gene Repression in Xenopus tropicalis, ENDOCRINOLOGY, 156(2), 735-744, FEB 2015
  61. Epithelial DLD-1 Cells with Disrupted E-cadherin Gene Retain the Ability to Form Cell Junctions and Apico-basal Polarity, CELL STRUCTURE AND FUNCTION, 40(2), 79-94, 2015
  62. Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring the juxtamembrane region and plakophilins, JOURNAL OF BIOCHEMISTRY, 158(4), 339-353, OCT 2015
  63. Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines, GENES TO CELLS, 20(12), 1059-1076, DEC 2015
  64. Targeted gene correction of RUNX1 in induced pluripotent stem cells derived from familial platelet disorder with propensity to myeloid malignancy restores normal megakaryopoiesis, EXPERIMENTAL HEMATOLOGY, 43(10), 849-857, OCT 2015
  65. TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS ( MVA) syndrome, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(4), 1461-1466, JAN 28 2014
  66. EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step, JOURNAL OF CELL BIOLOGY, 206(3), 347-356, AUG 4 2014
  67. FAST-id system for enrichment of cells with TALEN-induced mutations and large deletions, GENES TO CELLS, 19(5), 419-431, MAY 2014
  68. Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells, SCIENTIFIC REPORTS, 4, NOV 20 2014
  69. Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems, SCIENTIFIC REPORTS, 4, JUL 16 2014
  70. Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing, EXPERIMENTAL ANIMALS, 63(3), 349-355, JUL 2014
  71. Simple knockout by electroporation of engineered endonucleases into intact rat embryos, SCIENTIFIC REPORTS, 4, OCT 1 2014
  72. Sterol Side Chain Reductase 2 Is a Key Enzyme in the Biosynthesis of Cholesterol, the Common Precursor of Toxic Steroidal Glycoalkaloids in Potato, PLANT CELL, 26(9), 3763-3774, SEP 2014
  73. TALEN-induced gene knock out in Drosophila, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 86-91, JAN 2014
  74. Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies, SCIENTIFIC REPORTS, 4, AUG 27 2014
  75. Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 108-114, JAN 2014
  76. Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 115-121, JAN 2014
  77. Screening Methods to Identify TALEN-Mediated Knockout Mice, EXPERIMENTAL ANIMALS, 63(1), 79-84, JAN 2014
  78. Versatile strategy for isolating transcription activator-like effector nuclease-mediated knockout mutants in Caenorhabditis elegans, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 78-85, JAN 2014
  79. Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex, BMC BIOTECHNOLOGY, 14, NOV 18 2014
  80. Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona, DEVELOPMENT, 141(2), 481-487, JAN 15 2014
  81. Targeted mutagenesis in sea urchin embryos using TALENs, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 92-97, JAN 2014
  82. Germ Cell Mutations of the Ascidian Ciona intestinalis With TALE Nucleases, GENESIS, 52(5), 431-439, MAY 2014
  83. Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes, SCIENTIFIC REPORTS, 3, FEB 13 2013
  84. Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome, DEVELOPMENTAL BIOLOGY, 375(1), 79-91, MAR 1 2013
  85. Efficient Targeted Mutagenesis in Medaka Using Custom-Designed Transcription Activator-Like Effector Nucleases, GENETICS, 193(3), 739-+, MAR 2013
  86. High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos., Biol Open, 2, 448-452, 20130301
  87. Production of Sry knockout mouse using TALEN via oocyte injection, SCIENTIFIC REPORTS, 3, NOV 5 2013
  88. Quantitative assay for TALEN activity at endogenous genomic loci, BIOLOGY OPEN, 2(4), 363-367, APR 15 2013
  89. The 3 ' UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin, DEVELOPMENTAL BIOLOGY, 377(1), 275-283, MAY 1 2013
  90. Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 54(5), 535-545, JUN 2012
  91. Targeted disruption of exogenous EGFP gene in medaka using zinc-finger nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 54(5), 546-556, JUN 2012
  92. Equarin is involved as an FGF signaling modulator in chick lens differentiation, DEVELOPMENTAL BIOLOGY, 368(1), 109-117, AUG 1 2012
  93. Nucleosome exclusion from the interspecies-conserved central AT-rich region of the Ars insulator, JOURNAL OF BIOCHEMISTRY, 151(1), 75-87, JAN 2012
  94. Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases, NATURE COMMUNICATIONS, 3, AUG 2012
  95. HpSumf1 is involved in the activation of sulfatases responsible for regulation of skeletogenesis during sea urchin development, DEVELOPMENT GENES AND EVOLUTION, 221(3), 157-166, AUG 2011
  96. Implication of HpEts in Gene Regulatory Networks Responsible for Specification of Sea Urchin Skeletogenic Primary Mesenchyme Cells, ZOOLOGICAL SCIENCE, 27(8), 638-646, AUG 2010
  97. Targeted mutagenesis in the sea urchin embryo using zinc-finger nucleases, GENES TO CELLS, 15(8), 875-885, AUG 2010
  98. Dicer is Required for the Normal Development of Sea Urchin, Hemicentrotus pulcherrimus, ZOOLOGICAL SCIENCE, 27(6), 477-486, JUN 2010
  99. HpSulf, a heparan sulfate 6-O-endosulfatase, is involved in the regulation of VEGF signaling during sea urchin development, MECHANISMS OF DEVELOPMENT, 127(3-4), 235-245, MAR-APR 2010
  100. Mammalian arylsulfatase A functions as a novel component of the extracellular matrix, CONNECTIVE TISSUE RESEARCH, 51(5), 388-396, OCT 2010
  101. Suppressor of Hairless (Su(H)) is Required for Foregut Development in the Sea Urchin Embryo, ZOOLOGICAL SCIENCE, 26(10), 686-690, OCT 2009
  102. Role of the Nanos Homolog During Sea Urchin Development, DEVELOPMENTAL DYNAMICS, 238(10), 2511-2521, OCT 2009
  103. Analysis of cis-regulatory elements controlling spatio-temporal expression of T-brain gene in sea urchin, Hemicentrotus pulcherrimus, MECHANISMS OF DEVELOPMENT, 125(1-2), 2-17, JAN-FEB 2008
  104. RSF governs silent chromatin formation via histone H2Av replacement, PLOS GENETICS, 4(2), FEB 2008
  105. The Ars insulator facilitates I-SceI meganuclease-mediated transgenesis in the sea urchin embryo, DEVELOPMENTAL DYNAMICS, 237(9), 2475-2482, SEP 2008
  106. DNA variations within the sea urchin Otx gene enhancer, FEBS LETTERS, 581(27), 5234-5240, NOV 13 2007
  107. Unichrom, a novel nuclear matrix protein, binds to the Ars insulator and canonical MARs, ZOOLOGICAL SCIENCE, 23(1), 9-21, JAN 2006
  108. Developmental expression of HpNanos, the Hemicentrotus pulcherrimus homologue of nanos, GENE EXPRESSION PATTERNS, 6(5), 572-577, JUN 2006
  109. Organ-specific and age-dependent expression of insulin-like growth factor-I (IGF-I) mRNA variants: IGF-IA and IB mRNAs in the mouse, ZOOLOGICAL SCIENCE, 22(9), 1011-1021, SEP 2005
  110. The Otx binding site is required for the activation of HpOtxL mRNA expression in the sea urchin, Hemicentrotus pulcherrimus, Development= Growth and Differentiation, 46(1), 20040201
  111. Sexually dimorphic expression of a teleost homologue of Mullerian inhibiting substance during gonadal sex differentiation in Japanese flounder, Paralichthys olivaceus, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 322(2), 508-513, SEP 17 2004
  112. Identification of two teleost homologs of the Drosophila sex determination factor, transformer-2 in medaka (Oryzias latipes), MECHANISMS OF DEVELOPMENT, 121(7-8), 991-996, JUL 2004
  113. The Otx binding site is required for the activation of HpOtxL mRNA expression in the sea urchin, Hemicentrotus pulcherrimus, DEVELOPMENT GROWTH & DIFFERENTIATION, 46(1), 61-69, FEB 2004
  114. A new G-stretch-DNA-binding protein, Unichrom, displays cell-cycle-dependent expression in sea urchin embryos, DEVELOPMENT GROWTH & DIFFERENTIATION, 46(4), 335-341, AUG 2004
  115. Utilization of a particle gun DNA introduction system for the analysis of cis-regulatory elements controlling the spatial expression pattern of the arylsulfatase gene (HpArs) in sea urchin embryos, DEVELOPMENT GENES AND EVOLUTION, 213(1), 44-49, FEB 2003
  116. Abnormal spermatogenesis at low temperatures in the Japanese red-bellied newt, Cynops pyrrhogaster: Possible biological significance of the cessation of Spermatocytogenesis, MOLECULAR REPRODUCTION AND DEVELOPMENT, 66(1), 60-66, SEP 2003
  117. Low temperature promotes annexin V expression in newt testis, ZOOLOGICAL SCIENCE, 20(6), 733-735, JUN 2003
  118. T-brain homologue (HpTb) is involved in the archenteron induction signals of micromere descendant cells in the sea urchin embryo, DEVELOPMENT, 129(22), 5205-5216, NOV 2002
  119. Follicle-stimulating hormone is indispensable for the last spermatogonial mitosis preceding meiosis initiation in newts(Cynops pyrrhogaster), Biology of Reproduction, 66(1), 14-20, 20020401
  120. Promotion of cathepsin L activity in newt spermatogonial apopotosis induced by prolactin, FEBS Letters, 521(1-3), 43-46, 20020401
  121. Human recombinant stem cell factor promotes spermatogonial proliferation, but not meiosis initiation in organ culture of newt testis fragments, Biochemical and Biophysical Research Communications, 294(3), 695-699, 20020401
  122. Caspase activity in newt spermatogonial apoptosis induced by prolactin and cycloheximide., Molecular Reproduction and Development, 52(2), 209-214, 20010401
  123. Expression of a novel matrix metalloproteinase gene during Cynops early embryogenesis, Biochemical and Biophysical Research Communications, 288(2), 380-384, 20010401
  124. Cold Suppression of follicle-stimulating hormone activity on proliferation and survival of newt spermatogonia, General and Comparative Endocrinology, 122(3), 296-303, 20010401
  125. Mammalian follicle-stimulating hormone (FSH) and insulin-like growth factor I (IGF-I) up-regulate IGF-I gene expression in organ culture of newt testis, Molecular Reproduction and Development, 60(1), 56-64, 20010401
  126. Cloning of dynein Intermediate chain cDNA of the newt Cynops pyrrhogaster, DNA Research, 8(2), 81-84, 20010401
  127. Conversion from mitosis to meiosis-morphology and expression of PCNA and Dmc1 during newt spermatogenesis, Development= Growth and Differentiation, 42(2), 603-612, 20000401
  128. Prolactin induces apotosis in the penultimate spermatogonial stage of the testes in Japanese red-bellied Newt (Cynops pyrrhogaster), Endocrinology, 141(6), 2027-2032, 20000401
  129. Molecular cloning, functional characterization and gene expression of a follicle-stimulating hormone receptor in the testis of newt, Cynops pyrrhogaster, Biochemical and Biophysical Research Communications, 275(1), 121-128, 20000401
  130. Cloning of cDNA for Xenopus prolactin receptor and its metamorphic expression profile, Development= Growth and Differentiation, 42(2), 167-174, 20000401
  131. IGF-I, IGF-II and insulin promote differentiation of spermatogonia to primary spermatocytes in organ culture of newt testes, International Journal of Developmental Biology, 43(4), 343-348, 19990401
  132. Newt Rad51-Cloning of cDNA and analysis of gene expression during the spermatogenesis, Development= Growth and Differentiation, 41(4), 401-406, 19990401
  133. Cloning of cDNA for newt WT1 and the differential expression during spermatogenesis of the Japanese newt, Cynops pyrrhogaster, Development= Growth and Differentiation, 40(6), 599-608, 19980401
  134. Cloning and expression of a cDNA encoding a prolactin receptor of the Japanese red-bellied newt, Cynops pyrrhogaster, Zoological Science, 15(5), 741-747, 19980401
  135. Genesis of axial fiber of newt sperm-cDNA cloning and expression of a 29 kDa protein, a major component of the axial fiber, during the spermatogenesis, International Journal of Developmental Biology, 40, 1109-1118, 19960401
  136. Expression of activin beta subunit genes in Sertoli cells of newt testes, Biochemical and Biophysical Research Communications, 224, 451-456, 19960401
  137. Differential expression of annexin V during spermatogenesis in Cynops pyrrhogaster, Development Genes and Evolution, 206, 64-71, 19960401
  138. A long polypyrimidine-polypurine sequence in 5’ flanking region of arylsulfatase gene of sea urchin embryo, International Journal of Developmental Biology, 38, 337-344, 19940401
  139. cis-Elements and protein factors related to regulation of transcription of arylsulfatase gene during sea urchin development, Development= Growth and Differentiation, 35(6), 703-710, 19930401
  140. Cis-acting elements for proper ontogenic expression of arylsulfatase gene of sea urchin embryo, Development= Growth and Differentiation, 34(6), 719-729, 19920401

Publications such as books

  1. 標的遺伝子の改変を可能にする人工ヌクレアーゼの開発、化学工業, 2013
  2. 人工ヌクレアーゼを用いたゲノム編集, 2012, 07
  3. DNA Structure, Chromatin and Gene ExpressionChapter 14, "Involvement of nuclear matrix and chromatin loop formation in the function of insulators.", 2006, 04, 8178952289
  4. Involvement of nuclear matrix and chromatin loop formation in the function of insulators (DNA structure, chromatin and gene expression) , 2006, 04, 8178952289, 252
  5. 2001, Initiation of meiosis in organ culture of newt testes by IGF-I , Perspective in Comparative Endocrinolog, 2001
  6. 1998, 両生類の発生生物学 , 1998
  7. 両生類における精子分化機構. 「生殖細胞の発生と性分化」, 1988, 08
  8. 1997, Proliferation of newt spermatogonia in vitro and expression of activin β subunit genes by Sertoli Cells. , J. Reprod. Dev., 1997
  9. 1996, Expression of activin beta subunit and type II receptor genes during newt spermatogenesis. , Inhibin= Activin and Follistatin, 1996

Awards

  1. 2016年11月14日, Blue Flame Award, (NPO) Addgene
  2. 2013年09月11日, JB Award, 公益社団法人日本生化学会会長, Nucleosome exclusion from the interspecies conserved central AT-rich region of the Ars insulator.
  3. 1999年09月, Zoological Science Award, The Zoological Society of Japan, Cloning and expression of a cDNA encoding a prolactin receptor of the Japanese red-bellied newt, Cynops pyrrhogaster

Works, etc.

  1. 2017
  2. 2016
  3. 2016
  4. 2015
  5. 2015
  6. 2015
  7. 2015
  8. 2015
  9. 2014
  10. ・DNA結合ドメインを含むポリペプチド(特願2013-166768), 2013/08
  11. ・核酸挿入用ベクター(特願2013-230349), 2013/09
  12. ・PPRモチーフを利用したDNA結合性蛋白質の設計方法及びその利用(特願2013-88940), 2013/04
  13. ・ヌクレアーゼを発現させるためのベクターおよびベクターセット、ならびに変異導入を受けた細胞の取得方法(特願2013-098724), 2013/05
  14. ・新規DNA結合ドメインおよびそれを含む新規DNA切断酵素(特願2011-242250), 2011/11
  15. ・「数理生命科学ディレクター養成プログラム」取組報告書, 2007/04

External Funds

Acceptance Results of Competitive Funds

  1. Practical Research Project for Rare / Intractable Diseases, 2015/11/16, 2016/03/31
  2. KAKENHI, 2014, 2016
  3. 2014
  4. 2015
  5. 2014, 2017
  6. Health and Labour Sciences Research Grants, 2014/06/02, 2015/03/31
  7. 2013, 2015
  8. 2013
  9. 2012
  10. 2011
  11. 2008/04, 2009/03
  12. KAKENHI, Quantitative analysis of cell-cell interaction responsible for the robustness of development, 2009, 2011
  13. KAKENHI, Gene expression noise during cell differentiation and its regulation, 2008, 2010
  14. KAKENHI, 2008, 2009
  15. KAKENHI, Studies on the gene network responsible for the specific gene expression during development, 2005, 2007
  16. 2005
  17. KAKENHI, 2004, 2005
  18. KAKENHI, 2002, 2003
  19. KAKENHI, Switching Mechanism for Meiosis Initiation or Apoptosis in Spermatogonia, 2000, 2001
  20. KAKENHI, 2000, 2001
  21. 2000
  22. KAKENHI, 1998, 1999
  23. KAKENHI, Mechanism of apoptosis induction in amphibian spermatogonia by prolactin, 1997, 1999
  24. KAKENHI, 1996, 1996
  25. KAKENHI, 1995, 1995
  26. KAKENHI, 1995, 1995
  27. KAKENHI, Analysis of the mechanism underlying meiosis initiation using in vitro culture system., 1993, 1995
  28. KAKENHI, 2013, 2016
  29. KAKENHI, 2013, 2015

Social Activities

History as Peer Reviews of Academic Papers

  1. 2016, Nature Communications