広島大学

Basic Information

Academic Degrees

• Doctor of Agriculture, The University of Tokyo
• Master of Agriculture, The University of Tokyo

Research Fields

• Agricultural sciences;Agricultural chemistry;Applied biochemistry

Research Keywords

• biosensing
• fusion tag
• semiconductor
• affinity purification
• silicon-binding (silica-binding) protein
• biomineralization

Educational Activity

Course in Charge

1. 2024, Undergraduate Education, 1Term, Basic Engineering Computer Programming
2. 2024, Undergraduate Education, Second Semester, Basic Experiments in Chemistry
3. 2024, Undergraduate Education, 4Term, Basic life science
4. 2024, Undergraduate Education, First Semester, Training of Biotechnology I
5. 2024, Undergraduate Education, 4Term, Molecular BiologyIII
6. 2024, Undergraduate Education, Year, Graduation Thesis
7. 2024, Graduate Education (Master's Program) , 2Term, Environmental Biotechnology A
8. 2024, Graduate Education (Master's Program) , Academic Year, Seminar in Biotechnology
9. 2024, Graduate Education (Master's Program) , 1Term, Exercises in Biotechnology A
10. 2024, Graduate Education (Master's Program) , 2Term, Exercises in Biotechnology A
11. 2024, Graduate Education (Master's Program) , 3Term, Exercises in Biotechnology B
12. 2024, Graduate Education (Master's Program) , 4Term, Exercises in Biotechnology B
13. 2024, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biotechnology
14. 2024, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences

Research Activities

Academic Papers

1. ★, Oriented immobilization of antibodies on a silicon wafer using Si-tagged protein A, ANALYTICAL BIOCHEMISTRY, 385(1), 132-137, 20090201
   Link to Paper Search Results by DOI
2. ★, Single-step affinity purification of recombinant proteins using the silica-binding Si-tag as a fusion partner, PROTEIN EXPRESSION AND PURIFICATION, 71(1), 91-95, 2010
   Link to Paper Search Results by DOI
3. The silica-binding Si-tag functions as an affinity tag even under denaturing conditions, PROTEIN EXPRESSION AND PURIFICATION, 77(2), 173-177, 2011
   Link to Paper Search Results by DOI
4. ★, Why does the silica-binding protein Si-tag" bind strongly to silica surfaces? Implications of conformational adaptation of the intrinsically disordered polypeptide to solid surfaces", COLLOIDS AND SURFACES B-BIOINTERFACES, 86(2), 359-363, 2011
   Link to Paper Search Results by DOI
5. Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate:ferredoxin oxidoreductase from Hydrogenobacter thermophilus, FEBS Journal, 277(2), 501-510, 2010
6. Affinity purification of recombinant proteins using a novel silica-binding peptide as a fusion tag, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98(12), 5677-5684, 2014
   Link to Paper Search Results by DOI
7. ★, Automated enzyme-linked immunosorbent assay using beads in a single tip (BIST) technology coupled with a novel anchor protein for oriented antibody immobilization, ANALYTICAL METHODS, 6(16), 6232-6235, 2014
   Link to Paper Search Results by DOI
8. ★, The C-Terminal Zwitterionic Sequence of CotB1 Is Essential for Biosilicification of the Bacillus cereus Spore Coat, JOURNAL OF BACTERIOLOGY, 198(2), 276-282, 2016
   Link to Paper Search Results by DOI
9. ★, Application of volcanic ash particles for protein affinity purification with a minimized silica-binding tag, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 122(5), 633-638, 2016
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10. ★, Bacterial biosilicification: a new insight into the global silicon cycle, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 85(6), 1324-1331, 2021
    Link to Paper Search Results by DOI

Invited Lecture, Oral Presentation, Poster Presentation

1. The biosynthetic pathway of long-chain polyamines in the biosilicifying bacterium Bacillus cereus, Takeshi Ikeda, Takeshi Ikeda, Yukihide Nakasugi, Kohjiro Yamamoto, Ryuichi Hirota, Akio Kuroda, Biomin XVI/16th International Symposium on Biomineralization, 2021/08/25, Without Invitation, English
2. Discovery of long-chain polyamines and their biosynthetic enzyme in the biosilicifying bacterium Bacillus cereus, Takeshi Ikeda, Kohjiro Yamamoto, Ryuichi Hirota, Akio Kuroda, BIOMIN XV 15th International Symposium on Biomineralization, 2019/09/12, Without Invitation, English, Ludwig Maximilians University, Munich, Germany
3. Silica biomineralization in the gram-positive, spore-forming bacterium Bacillus cereus, Takeshi Ikeda, Miki Nakagawa, Kei Motomura, Ryuichi Hirota, Akio Kuroda, BiominXIV/14th International Symposium on Biomineralization, 2017/10/10, Without Invitation, English, Tsukuba International Congress Center
4. Development of a silicon-based immunosensor using the fusion protein of an antibody fragment and silicon-binding protein "Si-tag", Takeshi Ikeda, Kei Motomura, Yoshiteru Amemiya, Shin Yokoyama, Akio Kuroda, 24th Anniversary World Congress on Biosensors (Biosensors 2014), 2014/05/30, Without Invitation, English, Melbourne Convention and Exhibition Centre, Melbourne, Australia