TAKESHI IKEDA

Last Updated :2022/07/01

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Associate Professor
E-mail
ikedatakeshihiroshima-u.ac.jp

Basic Information

Academic Degrees

  • Doctor of Agriculture, The University of Tokyo
  • Master of Agriculture, The University of Tokyo

Research Fields

  • Agricultural sciences;Agricultural chemistry;Applied biochemistry

Research Keywords

  • biosensing
  • fusion tag
  • semiconductor
  • affinity purification
  • silicon-binding (silica-binding) protein
  • biomineralization

Educational Activity

Course in Charge

  1. 2022, Undergraduate Education, First Semester, Training of Biotechnology I
  2. 2022, Undergraduate Education, Second Semester, Basic Experiments in Chemistry
  3. 2022, Undergraduate Education, 4Term, Molecular BiologyIII
  4. 2022, Graduate Education (Master's Program) , Academic Year, Seminar in Biotechnology
  5. 2022, Graduate Education (Master's Program) , 1Term, Exercises in Biotechnology A
  6. 2022, Graduate Education (Master's Program) , 2Term, Environmental Biotechnology A
  7. 2022, Graduate Education (Master's Program) , 2Term, Exercises in Biotechnology A
  8. 2022, Graduate Education (Master's Program) , 3Term, Exercises in Biotechnology B
  9. 2022, Graduate Education (Master's Program) , 4Term, Exercises in Biotechnology B
  10. 2022, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biotechnology
  11. 2022, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences
  12. 2022, Undergraduate Education, Second Semester, Basic Experiments in Chemistry
  13. 2022, Undergraduate Education, First Semester, Training of Biotechnology I
  14. 2022, Undergraduate Education, 4Term, Molecular BiologyIII
  15. 2022, Graduate Education (Master's Program) , 2Term, Environmental Biotechnology A
  16. 2022, Graduate Education (Master's Program) , Academic Year, Seminar in Biotechnology
  17. 2022, Graduate Education (Master's Program) , 1Term, Exercises in Biotechnology A
  18. 2022, Graduate Education (Master's Program) , 2Term, Exercises in Biotechnology A
  19. 2022, Graduate Education (Master's Program) , 3Term, Exercises in Biotechnology B
  20. 2022, Graduate Education (Master's Program) , 4Term, Exercises in Biotechnology B
  21. 2022, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biotechnology
  22. 2022, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences

Research Activities

Academic Papers

  1. ★, Oriented immobilization of antibodies on a silicon wafer using Si-tagged protein A, ANALYTICAL BIOCHEMISTRY, 385(1), 132-137, 20090201
  2. ★, Single-step affinity purification of recombinant proteins using the silica-binding Si-tag as a fusion partner, PROTEIN EXPRESSION AND PURIFICATION, 71(1), 91-95, 2010
  3. The silica-binding Si-tag functions as an affinity tag even under denaturing conditions, PROTEIN EXPRESSION AND PURIFICATION, 77(2), 173-177, 2011
  4. ★, Why does the silica-binding protein Si-tag" bind strongly to silica surfaces? Implications of conformational adaptation of the intrinsically disordered polypeptide to solid surfaces", COLLOIDS AND SURFACES B-BIOINTERFACES, 86(2), 359-363, 2011
  5. Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate:ferredoxin oxidoreductase from Hydrogenobacter thermophilus, FEBS Journal, 277(2), 501-510, 2010
  6. Affinity purification of recombinant proteins using a novel silica-binding peptide as a fusion tag, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98(12), 5677-5684, 2014
  7. ★, Automated enzyme-linked immunosorbent assay using beads in a single tip (BIST) technology coupled with a novel anchor protein for oriented antibody immobilization, ANALYTICAL METHODS, 6(16), 6232-6235, 2014
  8. ★, The C-Terminal Zwitterionic Sequence of CotB1 Is Essential for Biosilicification of the Bacillus cereus Spore Coat, JOURNAL OF BACTERIOLOGY, 198(2), 276-282, 2016
  9. ★, Application of volcanic ash particles for protein affinity purification with a minimized silica-binding tag, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 122(5), 633-638, 2016
  10. ★, Bacterial biosilicification: a new insight into the global silicon cycle, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 85(6), 1324-1331, 2021

Invited Lecture, Oral Presentation, Poster Presentation

  1. Discovery of long-chain polyamines and their biosynthetic enzyme in the biosilicifying bacterium Bacillus cereus, Takeshi Ikeda, Kohjiro Yamamoto, Ryuichi Hirota, Akio Kuroda, BIOMIN XV 15th International Symposium on Biomineralization, 2019/09/12, Without Invitation, English, Ludwig Maximilians University, Munich, Germany
  2. Silica biomineralization in the gram-positive, spore-forming bacterium Bacillus cereus, Takeshi Ikeda, Miki Nakagawa, Kei Motomura, Ryuichi Hirota, Akio Kuroda, BiominXIV/14th International Symposium on Biomineralization, 2017/10/10, Without Invitation, English, Tsukuba International Congress Center
  3. Development of a silicon-based immunosensor using the fusion protein of an antibody fragment and silicon-binding protein "Si-tag", Takeshi Ikeda, Kei Motomura, Yoshiteru Amemiya, Shin Yokoyama, Akio Kuroda, 24th Anniversary World Congress on Biosensors (Biosensors 2014), 2014/05/30, Without Invitation, English, Melbourne Convention and Exhibition Centre, Melbourne, Australia