Hiroyuki Horiuchi

Last Updated :2024/05/07

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Professor
Web Site
E-mail
hhori10hiroshima-u.ac.jp
Self-introduction
*Basic research area: Avian stem cell biology, Avian immunobiology, Avian embryogenesis. Applied research area: Production of genetic-modified and genome editing domestic poultry. Study on elucidation of sex determination in chickens, highly pathogenic avian influenza (HPAIV), and development of useful chicken and/or mouse monoclonal antibodies (mAbs).

Basic Information

Major Professional Backgrounds

  • 1992/01/01, 2002/03/31, Hiroshima University, Faculty of Applied Biological Science, Research Assistant
  • 2007/04/01, 2010/03/31, Hiroshima University, Graduate School of Biosphere Science, Research associate
  • 2010/04/01, 2012/04/30, Hiroshima University, associate professor
  • 2012/05/01, Hiroshima University, professor

Educational Backgrounds

  • Hiroshima University, Graduate School, Division of Biophere Sciences, Japan, 1988/04, 1990/03
  • Hiroshima University, Faculty of Applied Biological Science, Japan, 1983/04, 1988/03

Academic Degrees

  • Doctor of Science, Hiroshima University
  • Master of Agriculture, Hiroshima University

Educational Activity

  • [Bachelor Degree Program] School of Applied Biological Science : Department of Applied Biological Science : Molecular Agro-Life Science Program
  • [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Food and AgriLife Science
  • [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Food and AgriLife Science

In Charge of Primary Major Programs

  • Molecular Agro-Life Science Program

Research Fields

  • Agricultural sciences;Animal life science;Integrative animal science

Research Keywords

  • chicken
  • thrombocyte
  • Cytokine
  • membrane protein
  • embryonic stem cells
  • pluripotent stem cell
  • germ cell
  • genome editing
  • dementia

Affiliated Academic Societies

  • The Molecular Biology Society of Japan, 1994/04
  • The Japanease Society for Genome Editing, 2016/04

Educational Activity

Course in Charge

  1. 2024, Undergraduate Education, Intensive, Laboratory Work in General Biology I
  2. 2024, Undergraduate Education, 1Term, Introduction to Physiology
  3. 2024, Undergraduate Education, 4Term, Research Front of Food and AgriLife Science
  4. 2024, Undergraduate Education, 2Term, Pathology
  5. 2024, Undergraduate Education, Intensive, Graduation Thesis
  6. 2024, Undergraduate Education, Second Semester, Graduation Thesis I
  7. 2024, Undergraduate Education, First Semester, Graduation Thesis II
  8. 2024, Undergraduate Education, Second Semester, Graduation Thesis III
  9. 2024, Undergraduate Education, 4Term, Systemic Life Science
  10. 2024, Undergraduate Education, Intensive, Laboratory Work in Molecular Agro-life ScienceIII
  11. 2024, Undergraduate Education, 1Term, Cell Technology
  12. 2024, Undergraduate Education, 4Term, Bio-Analytical Science
  13. 2024, Undergraduate Education, 3Term, Reading of Foreign Literature in Molecular Agro-Life Science
  14. 2024, Undergraduate Education, Intensive, (AIMS)Molecular Agro-life Science
  15. 2024, Undergraduate Education, 3Term, Molecular Agro-life Science
  16. 2024, Graduate Education (Master's Program) , 1Term, Exercises in Food andAgriLife Science A
  17. 2024, Graduate Education (Master's Program) , 2Term, Exercises in Food andAgriLife Science A
  18. 2024, Graduate Education (Master's Program) , 3Term, Exercises in Food andAgriLife Science B
  19. 2024, Graduate Education (Master's Program) , 4Term, Exercises in Food andAgriLife Science B
  20. 2024, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Food andAgriLife Science
  21. 2024, Graduate Education (Master's Program) , 2Term, Animal Life Science
  22. 2024, Graduate Education (Master's Program) , 4Term, Introduction to Genetics and Genomics
  23. 2024, Graduate Education (Doctoral Program) , Intensive, Internship at CiRA
  24. 2024, Graduate Education (Doctoral Program) , Intensive, Internship at Cutting-edge Genome Editing Laboratories
  25. 2024, Graduate Education (Doctoral Program) , Intensive, Internship at Collaborative Research Companies

Research Activities

Academic Papers

  1. ★, Chicken Interleukin-5 is Expressed in Splenic Lymphocytes and Affects Antigen-Specific Antibody Production, Journal of Poultry Science, 61, j, 61(jpsa2024002), on line-on line, 20240118
  2. ★, Detection limitations of prion seeding activities in blood samples from patients with sporadic prion disease, BMC Neurology, Open Access(Open Access), Open Access-Open Access, 20240311
  3. Evaluation of expression systems for recombinant protein production in chicken egg bioreactors, BIOTECHNOLOGY JOURNAL, 19(1), 202401
  4. ★, Wnt signaling blockade is essential for maintaining the pluripotency of chicken embryonic stem cells, Poultry Science, open access(open access), open access-open access, 20231207
  5. ★, Evaluation of expression systems for recombinant protein production in chicken egg bioreactors, Biotechnology Journal, 20231019
  6. ★, Lipofection with Lipofectamine™ 2000 in a heparin-free growth medium results in high transfection efficiency in chicken primordial germ cells, Biotechnology Journal, 20230804
  7. ★, Transcription activator-like effector nuclease-mediated deletion safely eliminates the major egg allergen ovomucoid in chickens, FOOD AND CHEMICAL TOXICOLOGY, 175, 202305
  8. ★, Fate Decisions of Chicken Primordial Germ Cells (PGCs): Development, Integrity, Sex Determination, and Self-Renewal Mechanisms, GENES, 14(3), 202303
  9. Screening of Optimal CpG-Oligodeoxynucleotide for Anti-Inflammatory Responses in the Avian Macrophage Cell Line HD11, Journal of Poultry Science, 60(1), 20230125
  10. ★, Genome editing in chickens, Gene and Genome Editing, 3-4, 1-8, 20221112
  11. ★, Prediction of sex-determination mechanisms in avian primordial germ cells using RNA-seq analysis, Scientific Reports, 12(13528), on line-on line, 20220817
  12. ★, Targeted Knock-in of a Fluorescent Protein Gene into the Chicken Vasa Homologue Locus of Chicken Primordial Germ Cells using CRIS-PITCh Method, The Journal of Poultry Science, 59(2), 182-190, 20220425
  13. ★, Knock-in of the duck retinoic acid-inducible gene I (RIG-I) into the Mx gene in DF-1 cells enables both stable and immune response-dependent RIG-I expression, Biochemistry and Biophysics Reports, 27(on line), on line-on line, 20210726
  14. Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice, plos pathogens, 20200826
  15. ★, An improved protocol for stable and efficient culturing of chicken primordial germ cells using small-molecule inhibitors, Cytotechnology, 72(3), 397-405, 20200601
  16. ★, Comparison of sex determination mechanism of germ cells between birds and fish: Cloning and expression analyses of chicken forkhead box L3-like gene., Developmental Dynamics, 248(9), 826-836, 20190902
  17. Zymosan A enhances humoral immune responses to soluble protein in chickens, JOURNAL OF VETERINARY MEDICAL SCIENCE, 79(8), 1335-1341, 201708
  18. Expression of ayu antimicrobial peptide genes after LPS stimulation, JOURNAL OF VETERINARY MEDICAL SCIENCE, 79(6), 1072-1080, 2017
  19. Induction of non-specific suppression in chicks by specific combination of maternal antibody and related antigen, JOURNAL OF VETERINARY MEDICAL SCIENCE, 77(11), 1363-1369, 2015
  20. Studies on Morphology and Cytochemistry in Blood Cells of Ayu Plecoglossus altivelis altivelis, JOURNAL OF VETERINARY MEDICAL SCIENCE, 76(5), 693-704, 2014
  21. ★, Manipulating the avian epiblast and epiblast-derived stem cell, Methods Mol. Biol., 1074, 151-173, 201311
  22. Molecular cloning of chicken interleukin-5 receptor alpha-chain and analysis of its binding specificity, DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 37(42798), 354-362, 2012
  23. ★, Characteristics of Novel Chicken Embryonic Stem Cells Established Using Chicken Leukemia Inhibitory Factor, JOURNAL OF POULTRY SCIENCE, 48(1), 64-72, 2011
  24. ★, Construction of an Insertion Vector for Gene Targeting of Chicken Lens-specific Gene, JOURNAL OF POULTRY SCIENCE, 47(2), 144-148, 2010
  25. Allergenicity study of EGFP-transgenic chicken meat by serological and 2D-DIGE analysis, FOOD AND CHEMICAL TOXICOLOGY, 48(5), 1302-1310, 2010
  26. Induction of immune suppression in the chick by an optimal dose of an immunizing antigen in the presence of its specific maternal antibody., J. Vet. Med. Sci., 72(3), 276-262, 20090601
  27. Prolonged suppression of chick humoral immune response by antigen specific maternal antibody., J. Vet. Med. Sci., 71(4), 417-424, 20090401
  28. Generation and characterization of chicken monoclonal antibodies against human LOX-1., MAbs., 1(4), 357-363, 20090401
  29. Prolonged Suppression of Chick Humoral Immune Response by Antigen Specific Maternal Antibody, JOURNAL OF VETERINARY MEDICAL SCIENCE, 71(4), 417-424, 2009
  30. Construction of chicken-mouse chimeric antibody and immunogenicity in mice, JOURNAL OF VETERINARY MEDICAL SCIENCE, 70(4), 397-400, 2008
  31. Chicken antibody against a restrictive epitope of prion protein distinguishes normal and abnormal prion proteins., Biologicals, 35(4), 303-308, 20071025
  32. ★, Cloning of the chicken interleukin-13 receptor alpha 2 gene and production of a specific monoclonal antibody., Developmental and Comparative Immunology, 31(4), 394-406, 20070510
  33. Development of recombinant chicken IgY from single chain fragment of variable region for diagnosis of BSE., Biologicals, 35(1), 31-34, 20070301
  34. ★, Antibody engineering and transgenic technology in chickens, Food and Food Ingredients Journal of Japan, 211(11), 948-954, 20061101
  35. ★, Effect of novel monoclonal antibodies on LIF-induced signaling in chicken blastodermal cells., Dev. Comp. Immunol., 30(5), 513-522, 20060401
  36. Characterization and expression analysis of a chicken interleukin-6 receptor alpha., Dev. Comp. Immunol., 30(4), 419-429, 20060401
  37. ★, Maintenance of chicken embryonic stem cells in vitro., In Methods in Molecular Biology (Embryonic Stem Cell Protocols; Second Edition Vol. 1), 329, 17-34, 20060401
  38. Humanization of chicken monoclonal antibody using phage-display system., Mol. Immunol., 43(6), 634-642, 20060201
  39. Tributyltin disturbs bovine adrenal steroidogenesis by two modes of action., Steroids, 70(14), 913-921, 20051201
  40. Inhibition of prion propagation in scrapie-infected mouse neuroblastoma cell lines using mouse monoclonal antibodies against prion protein., Biochem. Biophys. Res. Commun., 335(1), 197-204, 20050901
  41. Stable production of recombinant chicken antibody in CHO-K1 cell line., Biologicals, 33(3), 169-174, 20050901
  42. Biological activity of recombinant chicken interleukin-6 in chicken hybridoma cells., Vet. Immunol. Immunopathol., 106(42737), 97-105, 20050601
  43. Generation of a mouse monoclonal antibody against chicken interleukin-6., Hybridoma (Larchmt)., 24(2), 115-117, 20050501
  44. ★, Excision of foreign gene product with cathepsin D in chicken hepatoma cell line., Biochem. Biophys. Res. Commun., 330(2), 533-539, 20050501
  45. Characterization and expression analysis of the chicken interleukin-11 receptor alpha chain., Dev. Comp. Immunol., 29(4), 349-359, 20050401
  46. Genomic organization of the chicken T-cell receptor beta chain D-J-C region., The Journal of Veterinary Medical Science, 66(12), 1509-1515, 20041201
  47. Expression vectors for chicken-human chimeric antibodies, Biologicals, 32(4), 213-218, 20041201
  48. Cloning and characterization of a chicken leukemia inhibitory factor, Avian Immunology Research Group, 8, 80, 20040904
  49. Characteruzation and expression analysis of the chicken interleukin-11 receptor alpha., Avian Immunology Research Group, 8, 82, 20040904
  50. Biological activities of recombinant chicken interleukin-6 on chicken hybridoma cells., Avian Immunology Research Group, 8, 85, 20040904
  51. Establishment of a chicken monoclonal antibody panel against mammalian prion protein, The Journal of Veterinary Medical Science, 66(7), 807-814, 20040801
  52. ★, Chicken leukemia inhibitory factor maintains chicken embryonic stem cells in the undifferentiated state, The Journal of Biological Chemistry, 279(23), 24513-24520, 20040601
  53. Molecular cloning and characterization of chicken tumor necrosis factor (TNF)-superfamily ligands, CD30L and TNF-related apoptosis inducing ligand (TRAIL), The Journal of Veterinary Medical Science, 66(6), 643-650, 20040601
  54. ★, A monoclonal antibody against chicken thrombocytes reacts with the cells of thrombocyte lineage, The Journal of Veterinary Medical Science, 66(3), 243-250, 20040301
  55. Molecular study on chicken tumor necrosis factor receptor-II and tumor necrosis factor receptor-associated factor-5., Vet. Immunol. Immunopathol., 98(42737), 31-41, 20040301
  56. Chicken peripheral blood CD3+CD4-CD8- cells are regulated by endocrine and immune systems., J. Vet. Med. Sci., 66(2), 143-148, 20040201
  57. Immunobiology of chicken germinal center: II. Accumulation of apoptotic cells within the germinal center, Cell Tissue Res., 314(2), 215-221, 20031101
  58. Two expression vectors for the phage-displayed chicken monoclonal antibody, Journal of Immunological Methods, 280(42737), 157-164, 20030901
  59. Flow cytometric analysis of chicken NK activity and its use on the effect of restraint stress, Journal of Veterinary Medical Science, 65(9), 995-1000, 20030901
  60. Generation of antibodies against prionprotein by scrapie-infected cell immunization of PrP0/0 mice, HYBRIDOMA and HYBRIDOMICS, 22(4), 263-266, 20030801
  61. Development of Peyerユs Patch and Cecal Tonsil in Gut-Associated Lymphoid Tissues in the Chicken Embryo., J. Vet. Med. Sci., 65(5), 607-614, 20030501
  62. Immunobiology of chicken germinal center: I. Changes in surface Ig class expression in the chicken splenic germinal center after antigen stimulation, Developpmental and Comparative Immunology, 27(2), 159-166, 20030201
  63. Studies on chicken cytokines: cloning and application, 46(2), 137-149, 20021201
  64. ★, Cloning and characterization of a chicken platelet-derived growth factor B-chain cDNA., Dev. Comp. Immunol., 26(1), 73-83, 20020101
  65. Establishment of monoclonal antibodies against chicken GP130, Veterinary Immunology Symposium, 6, 106, 20010715
  66. ★, Characterization and expression of three forms of cDNA encoding chicken platelet-derived growth factor-A chain, Gene, 272(42737), 181-190, 20010711
  67. Role of bursin in the development of B lymphocytes in chicken embryonic Bursa of Fabricius, Developpmental and Comparative Immunology, 25(42861), 485-493, 20010601
  68. CD3+CD4-CD8- cells present in chicken peripheral blood are regulated by aging and stress, CURRENT PROGRESS ON AVIAN IMMUNOLOGY RESEARCH, 6, 315-321, 20001008
  69. Construction of recombinant chicken monoclonal antibody using phage display system, CURRENT PROGRESS ON AVIAN IMMUNOLOGY RESEARCH, 6, 335-340, 20001001
  70. Construction of recombinant monoclonal antibodies from a chicken hybridoma line secreting specific antibody., Cytotechnology, 32, 191-198, 20000401
  71. cDNA cloning and mRNA expression of PDGF in chicken thrombocyte, Proceedings of the International Veterinary Cytokine and Vaccine Conference, 234-237, 20000316
  72. Expression of chicken gp130 mRNA in various chicken organs and cells, Proceedings of the International Veterinary Cytokine and Vaccine Conference, 330-333, 20000316
  73. Chicken monoclonal antibodies against synthetic bovine prion protein peptide., J. Vet. Med. Sci., 60(6), 777-779, 19980601
  74. ★, Manipulation of chick embryo and production of transgenic chicken., 3(4), 39-44, 19980401
  75. Ontogeny of thrombocytes in chicken embryos, The challenges of Lower Vertebrates to Immunology, 99, 19971113
  76. Two chicken B cell lines resistant to ouabain for the production of chicken monoclonal antibodies, The Journal of Veterinary Medical Science, 58(11), 1053-1056, 19961101
  77. Identification of the gene encoding VP7 of serotype G3 equine rotavirus, Journal of Equine Science, 7(1), 17-20, 19960401
  78. Detection of growth factor(s) in chicken thrombocytes., Avian Immunology Research Group, 19940320
  79. ★, Vitronectin, 201-208, 19930401
  80. Establishment of chicken B hybridomas and their applications, Avian Immunology in Progress, 91-96, 19930401
  81. ★, Identification of fibronectin in chicken thrombocytes, Cell Structure and Function, 17(2), 93-98, 19920417
  82. Vibrio virulence factors and the quantitative analysis of cytotoxiicity elaborated by environmental isolates, Cytobios, 66(266-267), 143-151, 19910401
  83. ★, Preliminary evidence of growth factor(s) from chicken thrombocytes- Growth effects of chicken fibroblasts cultures, Japanese Journal of Veterinary Science, 52(3), 559-565, 19900601

Publications such as books

  1. 2021/02/08, Recent Advances and Applications of Genome Editing Technologies, Genome-editing, Sex-determining, Chicken, 2021, 2, Scholarly Book, Joint work, J, Hiroyuki Horiuchi, Mei Matsuzaki, Ryo Ezaki, ISBN978-4-7813-1586-7 C3045, 240, 7
  2. 2019/12/15, Standard Protocols on Genome Editing, Genome Editing for birds, chicken PGC, Yodosha, 2019, 12, Scholarly Book, Joint work, J, Ryo Ezaki, Mei Matsuzaki, Hiroyuki Horiuchi, ISBN978-4-7581-2244-3, 386, 364-371

Invited Lecture, Oral Presentation, Poster Presentation

  1. Effects of Wnt signaling regulation on chicken embryonic stem cells, Ryota Kjihara, Ryo Ezaki, Takumi Terada, Mei Matsuzaki, Hiroyuki Horiuchi, The 46th Annual Meeting of the Molecular Biology Society of Japan, 2023/12/08, Without Invitation, Japanese, Molecular Biology Society of Japan, Kobe
  2. Effect of cell cycle regulation on genome editing efficiency in chicken cells, Ryo Ikegaya, Tenkai Watanabe, Yuuta Ochi, Ryo Ezaki, Takumi Terada, Mei Matsuzaki, Hiroyuki Horiuchi, The 46th Annual Meeting of the Molecular Biology Society of Japan, 2023/12/08, Without Invitation, Japanese, Molecular Biology Society of Japan, Koube
  3. Transfection and genome editing in chicken primordial germ cells, Tenkai Watanabe, Ryo Ikegaya, Ryo Ezaki, Takumi Terada, Mei Matsuzaki, Hiroyuki Horiuchi, The 46th Annual Meeting of the Molecular Biology Society of Japan, 2023/12/07, Without Invitation, Japanese, Molecular Biology Society of Japan, Koube
  4. Toward building digital breeding in poultry, Hiroyuki Horiuchi, The 46th Annual Meeting of the Molecular Biology Society of Japan, 2023/12/06, With Invitation, Japanese, Molecular Biology Society of Japan, Koube
  5. Somatic Differentiation of Chicken Primordial Germ Cells Exhibits Partial Epithelial-to-Mesenchymal Transition (EMT)-like Features, Kennosuke Ichikawa, Yuzuha Motoe, Mei Matsuzaki, Ryo Ezaki, Hiroyuki Horiuchi, Mike McGrew, JAACT2023, 2023/12/01, Without Invitation, English, Japanese Association for Animal Cell Technology, Nagoya
  6. TranscriptionActivator-LikeEffectorNuclease-Mediated Deletion Safely Eliminates the Major Egg Allergen Ovomucoid in Chickens, Ryo Ezaki, Tetsushi Sakuma, Daisuke Kodama, Ryou Sasahara, Taichi Shiraogawa, Kennosuke Ichikawa, Mei Matsuzaki, Akihiro Handa, Takashi Yamamoto, Hiroyuki Horiuchi, JAACT2023, 2023/11/30, With Invitation, English, Japanese Association for Animal Cell Technology, Nagoya
  7. Identification of feminization-related potential factors and processes in chicken primordial germ cells, Kennosuke Ichikawa, Yoshiaki Nakamura, Hidemasa Bono, Ryo Ezaki, Mei Matsuzaki, Mike McGrew, Hiroyuki Horiuchi, 11th Avian Model Systems Meeting, 2023/09/11, Without Invitation, English, Hg3 Conferences Ltd, UK
  8. Measuring the efficacy of PCV2 vaccination in the herd pigs under the natural co-infection of PRRSV and PCV2 based on the dynamics of gene, immune resposes, and rate of PCV2 isolation, Ringo Rissar Siringo, Mazimpaka Eugene, Choonnasard Amonrat, Tasuku Hirooka, Taketo Wada, Eigo Sakamoto, Shunichi Honda, Hiroyuki Horiuchi, Tmaki Okabayashi, Asian Pig Veterinary Society Congress 2023, 2023/07/30, Without Invitation, English, Asian Pig Veterinary Society, Tiwan
  9. VULNERABILITY OF REAL-TIME PCR IN EVALUATING PORCINE CIRCOVIRUS TYPE 2 INFECTION AND ESTABLISHMENT OF A NOVEL DETECTION METHOD USING INVERSE PCR COMBINED WITH REAL-TIME PCR, Taketo Wada, Ryo Ezaki, Kennosuke Ichikawa, Mei Matsuzaki, Eigo Sakamoto, Shunichi Honda, Eugene Mazimpaka, Kazuki Takahashi, Tamaki Okabayashi, Hiroyuki Horiuchi, Asian Pig Veterinary Society Congress 2023, 2023/07/30, Without Invitation, English, Asian Pig Veterinary Society, Taipei Taiwan
  10. Characterization of differentiated chick primordial germ cells: Toward the establishment of a novel culture system for avian primordial germ cells, Kennosuke Ichikawa, Yuzuha Motoe, Ryo Ezaki, Mei Matsuzaki, Hiroyuki Horiuchi, The 45th Annual Meeting of the Molecular Biology Society of Japan, 2022/11/30, Without Invitation, Japanese, The Molecular Biology Society of Japan, Makuhari Messe, Chiba
  11. Can cultured chicken epiblast cells become pluripotent stem cells?, Ryota Kajihara, Kennosuke Ichikawa, Ryo Ezaki, Mei Matsuzaki, Hiroyuki Horiuchi, The 45th Annual Meeting of the Molecular Biology Society of Japan, 2022/11/30, Without Invitation, Japanese, The Molecular Biology Society of Japan, Makuhari Messe, Chiba
  12. Construction of an Evaluation System for Chicken Egg Bioreactor Using Cultured Chicken Cells, Ryota Kajihara, Kenosuke Ishikawa, Ryo Ezaki, Mei Matsuzaki, Hiroyuki Horiuchi, International Seminar 2022:Front-line of genome editing technology, 2022/11/12, Without Invitation, English, Hiroshima University, online
  13. Genetic Engineering in Chicken Primordial Germ Cells, Tenkai Watanabe, Kenosuke Ishikawa, Ryo Ezaki, Mei Matsuzaki, Hiroyuki Horiuchi, 2022/11/12, Without Invitation, English, Hiroshima University, online
  14. Effect of Porcine circovirus 2 (PCV-2) vaccine on PCV-2 viremia in vaccinated piglets under field conditions, Tamaki Okabayashi, Eugene Mazimpaka, Kazuki Takahashi, Taketo Wada, Hiroyuki Horiuchi, Akatsuki Saito, Masafumi Mori, Eigo Sakamoto, IPVS2022, 2022/06/21, Without Invitation, English, International Pig Veterinary Society, Rio de Janeiro (Brazil)
  15. Knock-in strategy by genome editing in chicken cells, Tenkai Watanabe, Yuta Ochi, Ryo Ezaki, Mei Matsuzaki, Hiroyuki Horiuchi, 7th Annual meeting of the Japanese society for Genome Editing, 2022/06/06, Without Invitation, Japanese, Japanese society for Genome Editing, on line & virtual
  16. Construction of an evaluation system for chicken egg bioreactor using cultured chicken cells, Ryota Kajihara, Ryo Ezaki, Mei Matsuzaki, Hiroyuki Horiuchi, 7th Annual meeting of the Japanese society Genome Editing, 2022/06/06, Without Invitation, Japanese, Japanese society Genome Editing, on line & virtual
  17. Study on the differentiation of chicken germ cells, Mayuko Yamawaki, Ryo Ezaki, Mei Matsuzaki, Hiroyuki Horiuchi, MBSJ2021, 2021/12/01, Without Invitation, English, The 45th Annual Meeting of the Molecular Biology Society of Japan (MBSJ2022), yokohama
  18. Construction of an evaluation system for chicken egg bioreactor using cultured chicken cells, Ryota Kajihara , Ryo Ezaki , Mei Matsuzaki , Hiroyuki Horiuchi, The 45th Annual Meeting of the Molecular Biology Society of Japan (MBSJ2022), 2021/12/01, Without Invitation, English, MBSJ2021 Secretariat c/o A & E Planning Co., Ltd, Yokohama
  19. Study on the differentiation of chicken germ cells., Mayuko Yamawaki, Ryo Ezaki, Mei Matsuzaki, Hiroyuki Horiuchi, The Japanese Society for Genome Editing 6th, 2021/06/18, Without Invitation, Japanese, The Japanese Society for Genome Editing, Fukuoka
  20. Study on host immune response via chicken MDA5 and LGP2, Kennosuke Ichikawa, Ryo Ezaki, Mei Matsuzaki, Hiroyuki Horiuchi, The Japanese Society for Genome Editing 6th, 2021/06/18, Without Invitation, Japanese, The Japanese Society for Genome Editing, Fukuoka
  21. Analyses of germ-cell fate determination in chickens, Hiroaki Matsumoto, Ryo Ezaki, Ryota Kajihara, Mei Matsuzaki, Shuichi Furusawa, Hiroyuki Horiuchi, 4th Annual Meeting of the Japanese Society for Genome Editing, 2019/06/05, Without Invitation, Japanese, The Japanese Society for Genome Editing, Tokyo
  22. Elucidation of mechanism of chicken sex determination using genome editing, Yuki Okaza, Mayuko Ymawaki, Ryo Ezaki, Mai Matsuzaki, Shuichi Furusawa, Hiroyuki Horiuchi, 4th Annual Meeting of the Japanese Society for Genome Editing, 2019/06/04, Without Invitation, Japanese, The Japanese Society for Genome Editing, Tokyo
  23. Study on biomarkers for diagnosis of rapidly progressive dementias, Yusuke Sanuki, Yumi Tanaka, Ryo Ezaki, Shuichi Furusawa, Noriyuki Nishida, Katsuya Satoh and Hiroyuki Horiuchi, The 41st Annual Meeting of the Molecular Biology Society of Japan, 2018/11/30, Without Invitation, Japanese, The Molecular Biology Society of Japan, Yokohama
  24. Forced-expression of target gene by insertion of promoter using PITCh system, Kosuke Yasui, Ryo Ezaki, Shuichi Furusawa and Hiroyuki Horiuchi, The 41st Annual Meeting of the Molecular Biology Society of Japan, 2018/11/29, Without Invitation, Japanese, The Molecular Biology Society of Japan, Yokohama
  25. Comparison of sex determination mechanism of germ cells between birds and fish by analyses of chicken foxl3., Kennosuke Ichikawa, Ryo Ezaki, Shuichi Furusawa and Hiroyuki Horiuchi, The 41st Annual Meeting of the Molecular Biology Society of Japan, 2018/11/28, Without Invitation, Japanese, The Molecular Biology Society of Japan, Yokohama
  26. Germ-cell differentiation induction of chicken pluripotent stem cells., Sachi Uehata, Asako Hirano, Ryo Ezaki, Shuichi Furusawa and Hiroyuki Horiuchi, The 41st Annual Meeting of the Molecular Biology Society of Japan, 2018/11/28, Without Invitation, Japanese, The Molecular Biology Society of Japan, Yokohama
  27. Functional analysis of DAZL in avian primordial germ cells using genome-editing tool, Kennosuke Ichikawa, Haruto Masaki, Ryo Ezaki, Shuichi Furusawa, Hiroyuki Horiuchi, The 3rd Annual Meeting of the Japanese Society for Genome Editing, 2018/06/20, Without Invitation, Japanese, The Japanese Society for Genome Editing, Hiroshima city
  28. Function analyses of sex determination related gene in chickens, Chikako Yamada, Yuki Nakagawa, Ryo Ezaki, Shuichi Furusawa, Hiroyuki Horiuchi, The 3rd Annual Meeting of the Japanese Society for Genome Editing, 2018/06/20, Without Invitation, Japanese, The Japanese Society for Genome Editing, Hiroshima city
  29. Functional analysis of DAZL in avian primordial germ cells using genome-editing tool, Haruto Masaki, Kennosuke Ichikawa, Ryo Ezaki, Shuichi Furusawa, Hiroyuki Horiuchi, The 3rd Annual Meeting of the Japanese Society for Genome Editing, 2018/06/19, Without Invitation, Japanese, The Japanese Society for Genome Editing, Hiroshima city
  30. Application of MMEJ-assisted gene knock-in by PITCh system in chickens, Ryo Ezaki, Fumiko Kameyama, Shuichi Furusawa, Hiroyuki Horiuchi, The 3rd Annual Meeting of the Japanese Society for Genome Editing, 2018/06/19, Without Invitation, Japanese, The Japanese Society for Genome Editing, Hiroshima city
  31. Functional analysis of DAZL in avian primordial germ cells, Haruto Masaki, Ryo Ezaki, Shuichi Furusawa, Hiroyuki Horiuchi, ConBio2017, 2017/12/08, Without Invitation, Japanese, The Molecular Biology Society of Japan, Kobe, JAPAN
  32. Study on avian sex determination using genome editing, Yuki Okaza, Ryo Ezaki, shuichi Furusawa, Hiroyuki Horiuchi, ConBio2017, 2017/12/08, Without Invitation, Japanese, The Molecular Biology Society of Japan, Kobe, JAPAN
  33. Isolation, culture and characterization of chicken amniotic mesenchymal stem cells, Karina Saheki, Ryo Ezaki, Fumiya Hirose, Shuichi Furusawa and Hiroyuki Horiuchi, ConBio2017, 2017/12/07, Without Invitation, English, The Molecular Biology Society of Japan, Kobe, JAPAN
  34. Elucidation of mechanism of avian sex determination using genome editing, Yuki Okaza, Yuki Nakagawa, Ryo Ezaki, shuichi Furusawa, Hiroyuki Horiuchi, The 2nd Annual Meeting of the Japanese Society for Genome Editing, 2017/09/29, Without Invitation, Japanese, the Japanese Society for Genome Editing, Osaka
  35. Cloning and expression analyses of chicken forkhead box L3, Kennosuke Ichikawa, Ryo Ezaki, Shuichi Furusawa, Hiroyuki Horiuchi, The Fourth World Congress of Reproductive Biology, 2017/09/27, Without Invitation, English, The Society for Reproduction and Development, Okinawa, Jaoan
  36. Development of poultry breeding technology using genome editinng, Hiroyuki Horiuchi, 2017/03/17, With Invitation, Japanese, Hiroshima
  37. Study on biomarkers for diagnosis of prion disease, Yumi Tanaka, Ryo Ezaki, Shuichi Furusawa, Noriyuki Nishida, Haruo Matsuda, Katusya Satoh, Hiroyuki Horiuchi, The 39th Annual Meeting of the Molecular Biology Society of Japan, 2016/12/01, Without Invitation, Japanese, Molecular Biology Society of Japan, Yokohama, Japan
  38. An improved protocol for stable and simple culturing of chicken primordial germ cells using apoptosis inhibitors, Ryo Ezaki, Fumiya Hirose, Shuichi Furusawa and Hiroyuki Horiuchi, The 39th Annual Meeting of the Molecular Biology Society of Japan, 2016/12/01, Without Invitation, Japanese, Molecular Biology Society of Japan, Yokohama, Japan
  39. Are chicken epiblast-derived stem cells naïve or primed pluripotent state?, Asako Hirono, Ryo Ezaki, Shuichi Furusawa, Hiroyuki Horiuchi, The 39th Annual Meeting of the Molecular Biology Society of Japan, 2016/11/30, Without Invitation, Japanese, Molecular Biology Society of Japan, Yokohama, Japan
  40. Cloning and expression analyses of chicken forkhead domain box L3, Kennosuke Ichikawa, Ryo Ezaki, Shuichi Furusawa, Hiroyuki Horiuchi, The 39th Annual Meeting of the Molecular Biology Society of Japan, 2016/11/30, Without Invitation, Japanese, Molecular Biology Society of Japan, Yokohama, Japan
  41. Genome editing in chicken primordial germ cell using genome editing tools, Yuki Nakagawa, Ryo Ezaki, Fumiya Hirose, Shuichi Furusawa, Tetsushi Sakuma, Asato Kuroiwa, Takashi Yamamoto, Hiroyuki Horiuchi, The 1st Annual Meeting of the Genome Editing Society of Japan, 2016/09/06, Without Invitation, Japanese, The Japanese Society of Genome Editing, Hiroshima, Japan
  42. Genome editing in chicken epiblast derived stem cells using CRISPR/Cas9, Fumiko Kameyama, Ryo Ezaki, Shuichi Furusawa, Hiroyuki Horiuchi, The 1st Annual Meeting of the Genome Editing Society of Jaopan, 2016/09/06, Without Invitation, Japanese, The Japanese Society of Genome Editing, Hiroshima. Japan
  43. Establishment and preservation of embryonic stem cells from Onagadori, Teruo Maeda, Qian Sun, Ryo Ezaki, Hiroyuki Horiuchi, The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress, 2016/08/22, Without Invitation, English, Fukuoka, JAPAN
  44. Stable and simple culture protocol for chicken primordial germ cells using apoptosis inhibitor, Ryo Ezaki, Fumiya Hirose, Shuichi Furusawa, Hiroyuki Horiuchi, The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress, 2016/08/22, Without Invitation, English, Fukuoka, JAPAN
  45. Establishment of quail ES-like cells, Mikiharu Nakano, Saki Soeda, Hiroyuki Horiuchi, Yoichi Matsuda, 2016 PSA Annual Meeting, 2016/07/11, Without Invitation, English, New Orleans, Louisiana, USA
  46. Establishment of high-sensitivity detection method of FABP3 in cerebrospinal fluid of CJD patients, Yumi Tanaka, Ryo Ezaki, Shuich Furusawa, Noriyuki Nishida, Haruo Matsuda, Katusya Satoh, Hiroyuki Horiuchi, PRION 2016, 2016/05/10, Without Invitation, English, Tokyo, JAPAN
  47. Establishment of quail ES-like cells, Mikiharu Nakano, Saki Soeda, Hiroyuki Horiuchi, Yoichi Matsuda, Avian Model Systems 9, 2016/03/28, Without Invitation, English, Taipei, TAIWAN
  48. Study on male sex determination gene in the bird using genome editing technology., Yuki Nakagawa, Ryo Ezai, Tetsushi Sakuma, Asato Kuroiwa, Takashi Yamamoto, Hiroyuki Horiuchi., Biochemistry and Molecular Biology 2015, 2015/12/04, Without Invitation, Japanese, Kobe, JAPAN

Awards

  1. 2023/12/01, JAACT2023 Oral Awardee, Japanese Association for Animal Cell Technology, Somatic Differentiation of Chicken Primordial Germ Cells Exhibits Partial Epithelial-to-Mesenchymal Transition (EMT)-like Features
  2. 2016/12/01, The 39th Annual Meeting of the Molecular Biology Society of Japan, Best Poster Award, The Molecular Biology Society of Japan, Are chicken epiblast-derived stem cells naive or primed pluripotent state?
  3. 2015/12/03, BMB2015 Young-Scientist Presentation Award, The Molecular Biology Society of Japan, Japanese Biochemical Society, Study on male sex determination gene in the chicken using genome editing technology
  4. 2011/10/26, 10th Transgenic Technology (TT) meeting,Best Poster Award, Dr. Lluis Montoliu ISTT Executive Committee, President, Florida (USA), October 24-26 (2011)で開催された10th Transgenic Technology (TT) meetingでポスター発表を行い,発表の内容,ポスターのレイアウト等が高く評価

Patented

  1. Patent, 6927496, 2021/08/10
  2. Patent, EP:2143791, 2014/04/23
  3. Patent, US:8198082, 2012/06/12
  4. Patent, US:7771714, 2010/08/10
  5. Patent, US:7691589, 2010/04/06
  6. Patent, US:7619080, 2009/11/17
  7. Patent, US:7250275, 2007/07/31
  8. Patent, EP:1264889, 2006/06/07

External Funds

Acceptance Results of Competitive Funds

  1. KAKENHI, 2013, 2016
  2. KAKENHI, Basic and Applied Study on Chicken Embryonic Stem Cell Lines, 2007, 2009
  3. KAKENHI, Establishment of chicken transgenic technology using embryonic stem cells., 2004, 2005
  4. KAKENHI, 2000, 2001
  5. KAKENHI, Analysis of T cell selection in the germinal center, 1999, 2000
  6. KAKENHI, 1996, 1996
  7. KAKENHI, 1995, 1995
  8. KAKENHI, 1994, 1994
  9. KAKENHI(Grant-in-Aid for Scientific Research (B)), 2019, 2022
  10. KAKENHI(Grant-in-Aid for Challenging Research (Exploratory)), 2019, 2021

Social Activities

Organizing Academic Conferences, etc.

  1. The 3rd Annual Meeting of The Japanese Society for Genome Editing, Organizaing committee member, 2017/07, 2018/07