MAKOTO HIRAYAMA

Last Updated :2024/06/06

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Associate Professor or Lecturer
Web Site
E-mail
hirayamahiroshima-u.ac.jp
Other Contact Details
1-4-4, Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, JAPAN, Japan
TEL : (+81) FAX : (+81)

Basic Information

Major Professional Backgrounds

  • 2005/04/01, 2006/03/31, Japan Society for the Promotion of Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Special Postdoctoral Researcher
  • 2008/11/01, 2016/03/31, Hiroshima University, Graduate School of Biosphere Science, Assistant Professor
  • 2006/04/01, 2007/03/31, Japan Society for the Promotion of Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Special Postdoctoral Researcher
  • 2016/04/01, 2019/03/31, Hiroshima University, Graduate School of Biosphere Science, Associate Professor
  • 2007/04/01, 2008/10/31, Japan Society for the Promotion of Science, Graduate School of Frontier Sciences, The University of Tokyo (20070401-20070831)/ Hopkins Marine Station, Stanford University (20070901-20081031), Special Postdoctoral Researcher

Educational Backgrounds

  • The University of Tokyo, Graduate School of Agricultural and Life Sciences, Department of Aquatic Bioscience, Japan, 2003/04, 2006/03
  • The University of Tokyo, Graduate School of Agricultural and Life Sciences, Department of Aquatic Bioscience, Japan, 2001/04, 2003/03
  • The University of Tokyo, Faculty of Agriculture, Major in Aquatic Life Sciences, Japan, 1999/04, 2001/03

Academic Degrees

  • Doctor of Agriculture, The University of Tokyo
  • Master of Agriculture, The University of Tokyo

Educational Activity

  • [Bachelor Degree Program] School of Applied Biological Science : Department of Applied Biological Science : Food Science Program
  • [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Food and AgriLife Science
  • [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Food and AgriLife Science

In Charge of Primary Major Programs

  • Food Science Program

Research Fields

  • Agricultural sciences;Applied aquatic science;Aquatic life science

Research Keywords

  • Algae, Bioactive compound, Lectin, Glycobiology, Molecular Biology, Anti-cancer, Anti-virus

Affiliated Academic Societies

Educational Activity

Course in Charge

  1. 2024, Liberal Arts Education Program1, 3Term, Cell Science
  2. 2024, Liberal Arts Education Program1, 3Term, Food safety and health science
  3. 2024, Undergraduate Education, Intensive, Laboratory Work in General Chemistry
  4. 2024, Undergraduate Education, 4Term, Research Front of Food and AgriLife Science
  5. 2024, Undergraduate Education, 1Term, Statistics in Biology
  6. 2024, Undergraduate Education, 4Term, Seafood Chemistry and Biochemistry
  7. 2024, Undergraduate Education, Second Semester, Graduation Thesis I
  8. 2024, Undergraduate Education, First Semester, Graduation Thesis II
  9. 2024, Undergraduate Education, Second Semester, Graduation Thesis III
  10. 2024, Undergraduate Education, 2Term, Reading of Foreign Literature in Food Science
  11. 2024, Undergraduate Education, 2Term, Bioresource Utilization Science
  12. 2024, Undergraduate Education, Intensive, Laboratory Work in Marine Bioresources Chemistry
  13. 2024, Undergraduate Education, Intensive, Training for Marine Food Processing
  14. 2024, Undergraduate Education, Intensive, (AIMS) Modern Food Science
  15. 2024, Undergraduate Education, 3Term, Modern Food Science
  16. 2024, Graduate Education (Master's Program) , 1Term, Exercises in Food andAgriLife Science A
  17. 2024, Graduate Education (Master's Program) , 2Term, Exercises in Food andAgriLife Science A
  18. 2024, Graduate Education (Master's Program) , 3Term, Exercises in Food andAgriLife Science B
  19. 2024, Graduate Education (Master's Program) , 4Term, Exercises in Food andAgriLife Science B
  20. 2024, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Food andAgriLife Science
  21. 2024, Graduate Education (Master's Program) , 2Term, Bioactive Natural Products Chemistry I
  22. 2024, Graduate Education (Master's Program) , 4Term, Bioactive Natural Products Chemistry II

Research Activities

Academic Papers

  1. Tetrodotoxin Detection in Japanese Bivalves: Toxification Status of Scallops, Marine Biotechnology, 20230117
  2. A marine sponge-derived lectin reveals hidden pathway for thrombopoietin receptor activation, NATURE COMMUNICATIONS, 13(1), 20221125
  3. Physiological response of the symbiotic dinoflagellate Pelagodinium beii to ultraviolet radiation: synthesis and accumulation of mycosporine-like amino acids, SYMBIOSIS, 86(3), 281-292, 202204
  4. High mannose-specific lectin (KAA-2) from the red alga Kappaphycus alvarezii potently inhibits influenza virus infection in a strain-independent manner, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 405(2), 291-296, 20110211
  5. High Mannose-binding Lectin with Preference for the Cluster of alpha 1-2-Mannose from the Green Alga Boodlea coacta Is a Potent Entry Inhibitor of HIV-1 and Influenza Viruses, JOURNAL OF BIOLOGICAL CHEMISTRY, 286(22), 19446-19458, 20110603
  6. Purification, Characterization, and cDNA Cloning of a Novel Lectin from the Green Alga, Codium barbatum, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 76(4), 805-811, 2012
  7. Purification , primary structure, and bioilogical activity of the high-mannose N-glycan specific lectin from cultivated Eucheuma denticulatum, JOURNAL OF APPLIED PHYCOLOGY, 27(4), 1657-1669, 2015
  8. Entry Inhibition of Influenza Viruses with High Mannose Binding Lectin ESA-2 from the Red Alga Eucheuma serra through the Recognition of Viral Hemagglutinin, MARINE DRUGS, 13(6), 3454-3465, 2015
  9. Biological activity, cDNA cloning and primary structure of lectin KSA-2 from the cultivated red alga Kappaphycus striatum (Schmitz) Doty ex Silva, PHYTOCHEMISTRY LETTERS, 14, 99-105, 2015
  10. High-Mannose Specific Lectin and Its Recombinants from a Carrageenophyta Kappaphycus alvarezii Represent a Potent Anti-HIV Activity Through High-Affinity Binding to the Viral Envelope Glycoprotein gp120, MARINE BIOTECHNOLOGY, 18(1), 144-160, 2016
  11. A Novel High-Mannose Specific Lectin from the Green Alga Halimeda renschii Exhibits a Potent Anti-Influenza Virus Activity through High-Affinity Binding to the Viral Hemagglutinin, MARINE DRUGS, 15(8), 2017

Publications such as books

  1. 2020/04/19, Lectin Purification and Analysis, Methods in Molecular Biology, High mannose (HM)-binding Oscillatoria agardhii agglutinin homologue (OAAH) lectin family is an important class of anti-viral proteins. The OAAH family lectins show potent anti-influenza virus activity with EC50 of nanomolar levels by binding to HM glycans of the envelope glycoprotein hemagglutinin (HA), thereby inhibiting the viral entry into host cells. No broadly effective neutralizing vaccines for influenza virus are available due to the frequent antigenic drift caused by rapid mutations. Alternatives for vaccines need to be developed to prepare for a possible risk of future emergence of a highly virulent virus. Possible use of antiviral lectins is a simple and useful strategy to prevent viral infection by interfering with the interaction between viral HA and the host sialic acid-containing receptor. High-density glycans of surface HA are primary targets for the lectins to inhibit viral entry. In general, the anti-influenza virus potency of lectins is evaluated by a series of inhibitory assays for infection, such as neutral red dye uptake assay to determine the extent of viral cytopathic effect, and immunofluorescence microscopy to detect the expression of viral proteins in infected cells. Direct interaction between lectins and HA could be evaluated by enzyme-linked immunosorbent assay or surface plasmon resonance analysis., The OAAH Family: Anti-Influenza Virus Lectins, Lectin Anti-virus, Humana Press, 2020, 4月, Scholarly Book, Joint work, E, Yuichiro Sato, Makoto Hirayama, Kinjiro Morimoto and Kanji Hori, 978-1-0716-0430-4, 693, 683-693
  2. 2014/07, Centrifugal Ultrafiltration-HPLC Method for Interaction Analysis Between Lectins and Sugars, in: Jun Hirabayashi (Ed.), Lectins-Methods and Protocols, Springer New York, Joint work, E

Invited Lecture, Oral Presentation, Poster Presentation

  1. Marine Algae-based Functional Ingredients for Foods and Nutraceuticals, Makoto Hirayama, THE 4th INTERNATIONAL CONFERENCE ON FOOD SCIENCE AND ENGINEERING (ICFSE) 2022, 2022/10/11, With Invitation, English, DEPT. OF FOOD SCIENCE AND TECHNOLOGY FACULTY OF AGRICULTURE, UNIVERSITAS SEBELAS MARET, Hybrid (Indonesia-Online)
  2. Inhibition of SARS-CoV-2 growth by algae-derived lectins, Tanuza Nazmul1, Toshihito Nomura2, Makoto Hirayama3, Yukiko Nakano3, Kanji Hori3, Takemasa Sakaguchi1 1Graduate School of Biomedical Sciences, Hiroshima University, 2Department of Infectious Diseases, Hiroshima University Hospital, 3Graduate School of Integrated Sciences for Life, Hiroshima University, Tanuza Nazmul1, Toshihito Nomura2, Makoto Hirayama3, Yukiko Nakano3, Kanji Hori3, Takemasa Sakaguchi1 1Graduate School of Biomedical Sciences, Hiroshima University, 2Department of Infectious Diseases, Hiroshima University Hospital, 3Graduate School of Integrated Sciences for Life, Hiroshima University, The 68th Annual Meeting of the Japanese Society for Virology, 2021/11/16, Without Invitation, English, The Japanese Society for Virology, Kobe, online
  3. Marine sponge lectin reveals the mechanism of sugar-chain mediated activation of MPL, The 83rd Annual Meeting of the Japanese Society of Hematology, 2021/09/23, Without Invitation, Japanese, Japanese Society of Hematology, online
  4. Fractionation and preparation of lectins and MAAs from a red alga Hypnea japonica, Makoto Hirayama, Hang Deng, Satoka Shigeoka, Kanji Hori, The 2021 Spring Meetings of JSFS, 2021/03/27, Without Invitation, Japanese, Japanese Society of Fisheries Science, Online
  5. A lectin-device is effective for trapping and clearance of HIV-1, Kanji Hori, Makoto Hirayama, Takemasa Sakaguchi, Hiroshi Kurokawa, Hiroyoshi Minakuchi, Kiyonori Hirota, Masahiro Iwakura, 23rd International Symposium on Glycoconjugates, 2015/09/17, Without Invitation, English, International Glycoconjugate Organization, Split, Croatia
  6. A novel high-mannose specific lectin from the calcareous green alga Halimeda renschii exhibits a strong anti-influenza virus activity, Jinmin Mu, Makoto Hirayama, Yuichiro Sato, Kinjiro Morimoto, Kanji Hori, Yuichiro Sato, Kinjiro Morimoto, The 2017 spring meeting of the Japanese Society of Fisheries Science, 2017/03/28, Without Invitation, English, Japanese Society of Fisheries Science, Tokyo University of Marine Science and Technology
  7. Structural and functional analyses of the lectins from the green algae Codium subtubulosum and C. latum, Makoto Hirayama, Haruka Shimazaki, Kanji Hori, 11th Asia Pacific Marine Biotechnology Conference, 2017/05/24, Without Invitation, English, The Asia Pacific Society of Marine Biotechnology, University of Hawaii at Manoa, Hawaii, USA
  8. Structural and functional analyses of a novel high-mannose specific lectin from an edible red alga Meristotheca papulosa, Makoto Hirayama, Suwako Chinen, Nana Kameda, Kanji Hori, International Symposium "Fisheries Science for Future Generations", 2017/09/24, Without Invitation, English, The Japanese Society of Fisheries Science, Tokyo University of Marine Science and Technology, Tokyo, Japan
  9. Diversity, Structure, Biological Functions of Algal Lectins, Makoto Hirayama, 36th Symposium and Annual Meeting of the Association of Sydtematic Biologists of the Philippines, 2018/05/31, With Invitation, English, Association of Systematic Biologists of the Philippines, Butuan City, Philippines
  10. Structure, Function and Diversity of Seaweed Lectins as Potential Taxonomic Markers, Makoto Hirayama, 2018/06/04, With Invitation, English, University of San Carlos, Cebu City, Philippines
  11. Diversity, Structure, and Biological Fucntions of Algal Lectins, Makoto Hirayama, 2018/06/01, With Invitation, English, Caraga State University, Butuan City, Philippines
  12. A novel complex type N-glycan-specific lectin from the calcareous green alga Halimeda borneensis exhibits a strong anti-influenza virus activity, Jinmin Mu, Makoto Hirayama, Kanji Hori (Graduate School of Biosphere Science, Hiroshima Univ.), Yuichiro Sato, Kinjiro Morimoto (Faculty of Pharmacy, Yasuda Women's Univ.), Yuichiro Sato, Kinjiro Morimoto (Faculty of Pharmacy, Yasuda Women's Univ.), 2018/09/16, Without Invitation, English

Patented

  1. 5892489, 2016/03/04
  2. Patent, 6624542, 2019/12/06

External Funds

Acceptance Results of Competitive Funds

  1. KAKENHI, Study of a transgenic technique in coenocytic green algae for the production of useful proteins, 2012, 2013
  2. KAKENHI, Study of a new transgenic technique in coenocytic green algae utilizing their protoplast regeneration ability, 2009, 2010
  3. KAKENHI, Characterization of coralline algae growth using genetic markers, 2011, 2013
  4. KAKENHI(Grant-in-Aid for Young Scientists (B)), 2017, 2019

Social Activities

History as Peer Reviews of Academic Papers

  1. 2020, Fisheries Science, Others, Reviewer, 1
  2. 2014, Fish Sci, Others, 1
  3. 2014, Fish Physiol Biochem, Others, 1
  4. 2013, Fish Sci, Others, 1
  5. 2015, Phycological Research, Others
  6. 2017, Aquaculture, Others
  7. 2018, Fisheries Science, Others, 1
  8. 2019, Fisheries Science, Others, 2