RYO EZAKI

Last Updated :2024/02/01

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Assistant Professor (Special Appointment)
E-mail
rezakihiroshima-u.ac.jp

Basic Information

Major Professional Backgrounds

  • 2006, WDB eureka co ltd., Research worker
  • 2008, Hiroshima Industrial Promotion Organization, Research worker
  • 2011, Hiroshima Biomedical Co., Research worker
  • 2013, The Graduate School of Biosphere Science, Research associate
  • 2019, Graduate School of Integrated Science of Life, Research associate
  • 2022, Graduate School of Integrated Science of Life, Specially appointed assistant professor

Educational Backgrounds

  • Hiroshima University, Bachelor of Agriculture, School of Applied Biological Science, 2004
  • Hiroshima University, Master of Agriculture, Graduate School of Biosphere Science, 2006
  • Hiroshima University, Ph.D. (Agriculture) in Graduate School of Integrated Science of Life, 2021

Academic Degrees

  • Hiroshima University

Research Activities

Academic Papers

  1. ★, Transcription activator-like effector nuclease-mediated deletion safely eliminates the major egg allergen ovomucoid in chickens, FOOD AND CHEMICAL TOXICOLOGY, 175, 202305
  2. Wnt signaling blockade is essential for maintaining the pluripotency of chicken embryonic stem cells, POULTRY SCIENCE, 20231207
  3. Evaluation of expression systems for recombinant protein production in chicken egg bioreactors, BIOTECHNOLOGY JOURNAL, 20231019
  4. Lipofection with Lipofectamine™ 2000 in a heparin-free growth medium results in high transfection efficiency in chicken primordial germ cells, BIOTECHNOLOGY JOURNAL, 20230804
  5. Screening of Optimal CpG-Oligodeoxynucleotide for Anti-Inflammatory Responses in the Avian Macrophage Cell Line HD11, JOURNAL OF POULTRY SCIENCE, 60(1), 202301
  6. Genome editing in chickens, GENE AND GENOME EDITING, 3-4, 1-8, 20221112
  7. Prediction of sex-determination mechanisms in avian primordial germ cells using RNA-seq analysis, SCIENTIFIC REPORTS, 12(1), 20220817
  8. Targeted Knock-in of a Fluorescent Protein Gene into the Chicken Vasa Homolog Locus of Chicken Primordial Germ Cells using CRIS-PITCh Method, JOURNAL OF POULTRY SCIENCE, 59(2), 182-190, 202204
  9. Knock-in of the duck retinoic acid-inducible gene I (RIG-I) into the Mx gene in DF-1 cells enables both stable and immune response-dependent RIG-I expression, BIOCHEMISTRY AND BIOPHYSICS REPORTS, 27, 20210726
  10. An improved protocol for stable and efficient culturing of chicken primordial germ cells using small-molecule inhibitors, CYTOTECHNOLOGY, 397-405, 20200229
  11. Comparison of sex determination mechanism of germ cells between birds and fish: Cloning and expression analyses of chicken forkhead box L3-like gene, DEVELOPMENTAL DYNAMICS, 248, 826-836, 201909
  12. Characteristics of Novel Chicken Embryonic Stem Cells Established Using Chicken Leukemia Inhibitory Factor., JOURNAL OF POULTRY SCIENCE, 48(1), 64-72, 2011
  13. Allergenicity study of EGFP-transgenic chicken meat by serological and 2D-DIGE analysis., FOOD AND CHEMICAL TOXICOLOGY, 1302-1310, 2010

Invited Lecture, Oral Presentation, Poster Presentation

  1. Functional analysis of DAZL in avian primordial germ cells using genome-editing tool, Kennosuke Ichikawa, Haruto Masaki, Ryo Ezaki, Shuichi Furusawa, Hiroyuki Horiuchi, 2018/06/20, Without Invitation, Japanese
  2. Isolation, culture and characterization of chicken amniotic mesenchymal stem cells, Karina Saheki, Ryo Ezaki, Fumiya Hirose, Shuichi Furusawa, Hiroyuki Horiuchi, 2017/12/07, Without Invitation, English
  3. Study on male sex determination gene in the bird using genome editing technology, Yuki Nakagawa, Ryo Ezaki, Tetsushi Sakuma, Asato Kuroiwa, Takashi Yamamoto, Hiroyuki Horiuchi, Biochemistry and Molecular Biology 2015, 2015/12/04, Without Invitation, Japanese, Biochemistry and Molecular Biology, Kobe
  4. Genetic Engineering in Chicken Primordial Germ Cells, Tenkai Watanabe, Kenosuke Ishikawa, Ryo Ezaki, Mei Matsuzaki, Hiroyuki Horiuchi, International Seminar 2022:Front-line of genome editing technology, 2022/11/12, Without Invitation, English, Hiroshima University, online
  5. Construction of an Evaluation System for Chicken Egg Bioreactor Using Cultured Chicken Cells, Ryota Kajihara, Kenosuke Ishikawa, Ryo Ezaki, Mei Matsuzaki, Hiroyuki Horiuchi, International Seminar 2022:Front-line of genome editing technology, 2022/11/12, Without Invitation, English, Hiroshima University, online
  6. VULNERABILITY OF REAL-TIME PCR IN EVALUATING PORCINE CIRCOVIRUS TYPE 2 INFECTION AND ESTABLISHMENT OF A NOVEL DETECTION METHOD USING INVERSE PCR COMBINED WITH REAL-TIME PCR, Taketo Wada, Ryo Ezaki, Kennosuke Ichikawa, Mei Matsuzaki, Eigo Sakamoto, Shunichi Honda, Eugene Mazimpaka, Kazuki Takahashi, Tamaki Okabayashi, Hiroyuki Horiuchi, Asian Pig Veterinary Society Congress 2023, 2023/07/30, Without Invitation, English, Asian Pig Veterinary Society, Taipei Taiwan
  7. Identification of feminization-related potential factors and processes in chicken primordial germ cells, Kennosuke Ichikawa, Yoshiaki Nakamura, Hidemasa Bono, Ryo Ezaki, Mei Matsuzaki, Mike McGrew, Hiroyuki Horiuchi, 11th Avian Model Systems Meeting, 2023/09/11, Without Invitation, English, Hg3 Conferences Ltd, UK
  8. Transcription Activator-Like Effector Nuclease-Mediated Deletion Safely Eliminates the Major Egg Allergen Ovomucoid in Chickens, Ryo Ezaki, Tetsushi Sakuma, Daisuke Kodama, Ryou Sasahara, Taichi Shiraogawa, Kennosuke Ichikawa, Mei Matsuzaki, Akihiro Handa, Takashi Yamamoto, Hiroyuki Horiuchi, JAACT2023, 2023/11/28, With Invitation, English, Japanese Association for Animal Cell Technology, Nagoya
  9. Somatic Differentiation of Chicken Primordial Germ Cells Exhibits Partial Epithelial-to-Mesenchymal Transition (EMT)-like Features, Kennosuke Ichikawa, Yuzuha Motoe, Mei Matsuzaki, Ryo Ezaki, Hiroyuki Horiuchi, Mike McGrew, JAACT2023, 2023/12/01, Without Invitation, English, Japanese Association for Animal Cell Technology, Nagoya
  10. Cloning and expression analyses of chicken forkhead box L3, The Fourth World Congress of Reproductive Biology, The Society for Reproduction and Development, 2017, Without Invitation, English, Okinawa, JAPAN
  11. Establishment of high-sensitivity detection method of FABP3 in cerebrospinal fluid of CJD patients., PRION 2016, 2016, Without Invitation, English, Tokyo, JAPAN
  12. Stable and simple culture protocol for chicken primordial germ cells using apoptosis inhibitor., The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress, 2016, Without Invitation, English, Fukuoka, JAPAN
  13. Establishment and preservation of embryonic stem cells from Onagadori., The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress, 2016, Without Invitation, English, Fukuoka, JAPAN

Patented

  1. Patent, 6927496, 2021/08/10
  2. Patent, 6754966, 2020/08/27