MASATSUGU MIYARA

Last Updated :2026/09/02

Affiliations, Positions
Graduate School of Biomedical and Health Sciences, Associate Professor
Web Site
https://kotakelab.hiroshima-u.ac.jp/homepage/
E-mail
miyara128hiroshima-u.ac.jp
Self-introduction
I investigate the mechanism underlying the toxicity of Parkinsonian neurotoxins (e.g., MPP+ and rotenone) by focusing on the function of organelles (e.g., lysosomes and mitochondria).

Basic Information

Major Professional Backgrounds

  • 2026/04/01, Hiroshima University, Graduate School of Biomedical and Health Sciences, Associate Professor
  • 2021/04/01, 2026/03/31, Hiroshima University, Graduate School of Biomedical and Health Sciences, Assistant Professor
  • 2018/04/01, 2021/03/31, Japan Society for the Promotion of Science, Research Fellow (PD)
  • 2017/04/01, 2018/03/31, Hiroshima University, Institute of Biomedical & Health Sciences, Postdoctoral Researcher
  • 2016/04/01, 2017/03/31, Hiroshima University, Special Postdoctoral Researcher
  • 2014/04/01, 2016/03/31, Japan Society for the Promotion of Science, Research Fellow (DC2)

Educational Backgrounds

  • Hiroshima University, Japan, 2012/04/01, 2016/03/23
  • Hiroshima University, Graduate School of Biomedical Sciences, Japan, 2010/04/01, 2012/03/23
  • Prefectural University of Hiroshima, Japan, 2006/04/01, 2010/03/19

Academic Degrees

  • Hiroshima University
  • Hiroshima University

Research Fields

  • Medicine,dentistry, and pharmacy;Pharmacy;Environmental and hygienic pharmacy
  • Medicine,dentistry, and pharmacy;Pharmacy;Biological pharmacy
  • Biology;Biological Science;Cell biology
  • Biology;Biological Science;Molecular biology

Research Keywords

  • Parkinson's disease
  • MPTP/MPP+
  • Tributyltin
  • Autophagy
  • Lysosome

Affiliated Academic Societies

  • Japan Society for Cell Biology
  • The Molecular Biology Society of Japan
  • The Japanese Society for Neurochemistry
  • The Japanese Society of Toxicology
  • The Pharmaceutical Society of Japan

Educational Activity

Course in Charge

  1. 2026, Undergraduate Education, 2Term, Public Health Chemistry II
  2. 2026, Undergraduate Education, First Semester, Experiments of Environmental Health Science
  3. 2026, Graduate Education (Doctoral Program) , Second Semester, Advanced Research in Pharmaceutical Science

Research Activities

Academic Papers

  1. Linalool inhalation-induced anxiety-like effects occur independently of olfactory input and require cytochrome P450 activity, JOURNAL OF TOXICOLOGICAL SCIENCES, 51(7), 393-402, 2026
  2. ★, Tributyltin induces conjugation of ATG8s to single membranes via the V-ATPase-ATG16L1 axis, leading to transcription factor EB activation in human cell lines, ARCHIVES OF TOXICOLOGY, 100(5), 1941-1951, 202605
    Link to Paper Search Results by DOI
  3. Linalool inhalation in mice leads to brain accumulation and metabolite-driven neuronal activation, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 786, 20251030
    Link to Paper Search Results by DOI
  4. Mice deficient in the phosphatase activity of sEH show decreased levels of the endocannabinoid 2-AG in the olfactory bulb and depressive-like behavior, FEBS Letters, 2024
    Link to Paper Search Results by DOI
  5. Photodegradation profiling of nitrendipine: evaluation of active pharmaceutical ingredient, tablets and its altered forms, ANALYTICAL SCIENCES, 39(11), 1791-1803, 202311
    Link to Paper Search Results by DOI
  6. Evaluation of photostability of azelnidipine tablets and structure determination of its photoproducts, JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 233, 20230905
    Link to Paper Search Results by DOI
  7. Tributyltin activates the Keapl-Nrf2 pathway via a macroautophagy-independent reduction in Keapl, JOURNAL OF TOXICOLOGICAL SCIENCES, 48(3), 161-168, 202303
    Link to Paper Search Results by DOI
  8. Methylmercury Decreases AMPA Receptor Subunit GluA2 Levels in Cultured Rat Cortical Neurons, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 46(2), 292-300, 202302
    Link to Paper Search Results by DOI
  9. ★, Trehalose decreases mRNA and protein expressions of c-Jun and JunB in human cervical cancer HeLa cells, JOURNAL OF BIOCHEMISTRY, 172(3), 177-187, 20220905
    Link to Paper Search Results by DOI
  10. ★, Tributyltin inhibits autophagy by decreasing lysosomal acidity in SH-SY5Y cells, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 592, 31-37, 20220212
    Link to Paper Search Results by DOI
  11. ★, MiT/TFE family members suppress L-leucyl-L-leucine methyl ester-induced cell death, The Journal of Toxicological Sciences, 46(3), 143-156, 2021
    Link to Paper Search Results by DOI
  12. Triphenyltin inhibits GA-binding protein α nuclear translocation, Fundamental Toxicological Sciences, 7(1), 33-40, 2020
    Link to Paper Search Results by DOI
  13. Carbofuran causes neuronal vulnerability to glutamate by decreasing GluA2 protein levels in rat primary cortical neurons, Archives of Toxicology, 92(1), 401-409, 201806
    Link to Paper Search Results by DOI
  14. Low-concentration tributyltin decreases GluR2 expression via nuclear respiratory factor-1 inhibition, International Journal of Molecular Sciences, 18(8), 1754, 20170811
    Link to Paper Search Results by DOI
  15. ★, Mild MPP+ exposure-induced glucose starvation enhances autophagosome synthesis and impairs its degradation, Scientific Reports, 7, 46668, 20170426
    Link to Paper Search Results by DOI
  16. Prenatal exposure to tributyltin decreases GluR2 expression in the mouse brain, Biological and Pharmaceutical Bulletin, 40(7), 1121-1124, 2017
    Link to Paper Search Results by DOI
  17. ★, Mild MPP+ exposure impairs autophagic degradation through a novel lysosomal acidity-independent mechanism, Journal of Neurochemistry, 139(2), 294-308, 201610
    Link to Paper Search Results by DOI
  18. Protein extracts from cultured cells contain nonspecific serum albumin, Bioscience, Biotechnology, and Biochemistry, 80(6), 1164-1167, 201606
    Link to Paper Search Results by DOI
  19. Methoxychlor and fenvalerate induce neuronal death by reducing GluR2 expression, The Journal of Toxicological Sciences, 41(2), 255-264, 201604
    Link to Paper Search Results by DOI
  20. Endogenous neurotoxic dopamine derivative covalently binds to Parkinson's disease-associated ubiquitin C-terminal hydrolase L1 and alters its structure and function, Journal of Neurochemistry, 130(6), 826-838, 201409
    Link to Paper Search Results by DOI
  21. Tributyltin-induced endoplasmic reticulum stress and its Ca(2+)-mediated mechanism, Toxicology and Applied Pharmacology, 272(1), 137-146, 201310
    Link to Paper Search Results by DOI
  22. Involvement of decreased glutamate receptor subunit GluR2 expression in lead-induced neuronal cell death, The Journal of Toxicological Sciences, 38(3), 513-521, 2013
    Link to Paper Search Results by DOI

Invited Lecture, Oral Presentation, Poster Presentation

  1. Mechanistic analysis of CCCP-induced protein insolubilization under low-pH conditions, 2026/08, Without Invitation, Japanese
  2. Exploration of neuronal lysosomal membrane damage response factors focusing on proteininsolubilization, 2026/08, Without Invitation, Japanese
  3. Tributyltin induces conjugation of ATG8s to single membranes and TFEB activation in human cell lines, 2026/07, Without Invitation, Japanese
  4. CASM-mediated stress response to tributyltin-induced lysosomal dysfunction, 2026/07, Without Invitation, Japanese
  5. Establishment of a new method for identifying lysosomal membrane damage response proteins, 2025/09, Without Invitation, Japanese
  6. Mechanism of p62 nuclear accumulation induced by a Parkinson's disease-related neurotoxin, 2025/09, Without Invitation, Japanese
  7. Mitochondrial uncouplers induce protein insolubilization under low pH conditions, 2025/09, Without Invitation, Japanese
  8. Characterization of human neuroblastoma cell line IMR-32 as a neuronal differentiation model for in vitro DNT analysis, 2025/09, Without Invitation, Japanese
  9. Novel lysosomal damage response mediated by lysosomal protein insolubilization, 2025/07, With Invitation, Japanese
  10. Protein insolubilization induced by chemical exposure under NaHCO3-depleted conditions, 2025/07, Without Invitation, Japanese
  11. Insolubilization of lysosomal proteins induced by lysosomal membrane damage, 2025/07, Without Invitation, Japanese
  12. Tributyltin induces non-canonical autophagy via V-ATPase-ATG16L1 association, 2025/07, Without Invitation, Japanese
  13. Study on the pathway for removal of damaged lysosomes via insolubilization of lysosomal proteins, 2025/07, Without Invitation, Japanese
  14. Tributyltin-induced LC3-II accumulation and its mechanism, 2025/03, Without Invitation, Japanese
  15. Insolubilization of lysosomal hydrolases induced by lysosomal membrane damage and its mechanism, 2025/03, Without Invitation, Japanese
  16. Mechanism of Keap1 protein loss induced by tributyltin, 2025/03, Without Invitation, Japanese
  17. Comprehensive search for genes that vary across three developmental neurotoxicants, Kotake Y, Fujihara R, Oguro A, Yamamoto Y, Tahara H, Miyara M, The 58th Congress of the European Societies of Toxicology (EUROTOX 2024), 2024/09, Without Invitation, English, Copenhagen
  18. Transcriptome analysis in the substantia nigra of a chronic MPTP mouse model of Parkinson's disease, 2024/09, Without Invitation, Japanese
  19. Elucidation of the mechanism of lysosomal hydrolase insolubilization during lysosomal membrane damage, 2024/09, Without Invitation, Japanese
  20. Search for genes affecting the toxicity of the dopaminergic neurotoxin MPP+, Suzuki F, Miyara M, Kotake Y, 2024 Japan/Korea Joint Symposium on Pharmaceutical Health Science and Environmental Toxicology, 2024/09, Without Invitation, English, Sendai
  21. Identification of genes involved in the toxicity of Parkinson's disease-associated neurotoxin MPP+, 2024/03, Without Invitation, Japanese
  22. Cytotoxicity evaluation and synthetic utilization of trioctyltin compounds, 2024/03, Without Invitation, Japanese
  23. Low concentrations of MPP+ promote glucose metabolism and impair autophagy, 2023/09, With Invitation, Japanese
  24. Tributyltin induces LC3-II accumulation independent of autophagy initiation complex, 2023/09, Without Invitation, Japanese
  25. Discovery of p62 nuclear accumulation induced by a Parkinson's disease-related neurotoxin and a novel nuclear role of p62, 2023/09, Without Invitation, Japanese
  26. Investigation of the mechanism of insolubilization of intralysosomal hydrolases induced by the lysosomal membrane-damaging agent LLOMe, 2023/09, Without Invitation, Japanese
  27. Search for genes involvbed in susceptibility to Parkinson's disease-associated neurotoxin MPP+, 2023/09, Without Invitation, Japanese
  28. Exploring mRNA markers for assessing developmental neurotoxicity using primary rat neurons, Fujihara R, Miyara M, Takehara Y, Takabayashi T, Yamamoto Y, Tahara H, Kotake Y, 2023/09, Without Invitation, English
  29. Autophagy dysfunction and p62 nuclear accumulation induced by low concentrations of a parkinsonian neurotoxin, 2022/11, With Invitation, Japanese
  30. Exploration of nuclear degradation pathway of a lysosomal stress-responsive transcription factor TFEB, 2022/08, Without Invitation, Japanese
  31. Mechanism of tributyltin-induced LC3-II accumulation, 2022/08, Without Invitation, Japanese
  32. Investigation of the effects of parkinsonian neurotoxin 1BnTIQ on the autophagy-lysosome system, 2022/08, Without Invitation, Japanese
  33. Protein insolubilization induced by mitochondrial dysfunction during starvation, 2022/08, Without Invitation, Japanese
  34. TBT activates Keap1-Nrf2 pathway via Keap1 decrease, 2022/08, Without Invitation, Japanese
  35. Tributyltin accumulates LC3-II via lysosomal dysfunction, Hatamiya S, Miyara M, Kotake Y, 2022/08, Without Invitation, English, Kumamoto
  36. Tributyltin decreases lysosomal acidity and inhibits autophagic degradation, Hatamiya S, Miyara M, Kotake Y, The 8th International Symposium on Metallomics, 2022/07, Without Invitation, English, Kanazawa
  37. Parkinsonian neurotoxin 1BnTIQ inhibits autophagy via lysosomal impairment, 2022/03, Without Invitation, Japanese
  38. Changes in protein properties induced by mitochondrial dysfunction during starvation, 2022/03, Without Invitation, Japanese
  39. Tributyltin activates Nrf2 via Keap1 reduction, 2022/03, Without Invitation, Japanese
  40. Global transcriptome analysis in low concentration of MPP+-induced cellular model of Parkinson's disease, Natsumi Okada, Masatsugu Miyara, Kanae Miyara, Miyuki Kanda, Hidetoshi Tahara, Yaichiro Kotake, 2021/09, Without Invitation, Japanese
  41. Tributyltin inhibits autophagy via lysosomal dysfunction, Shunichi Hatamiya, Masatsugu Miyara, Yaichiro Kotake, 2021/07, Without Invitation, Japanese
  42. Mild parkinsonian neurotoxin MPP+ exposure disrupts lysosomal gene expression, Natsumi Okada, Masatsugu Miyara, Kanae Miyara, Shunichi Hatamiya, Yusuke Kojima, Saya Takao, Yaichiro Kotake, 2021/03, Without Invitation, Japanese
  43. Tributyltin impairs autophagy, Shunichi Hatamiya, Masatsugu Miyara, Yaichiro Kotake, 2021/03, Without Invitation, Japanese
  44. Mild parkinsonian neurotoxin exposure induces autophagy dysfunction and nuclear p62 accumulation, Masatsugu Miyara, Wataru Tokunaga, Yuka Kuwabara, Namiko Watanabe, Shunichi Hatamiya, Tsuyoshi Nakanishi, Yaichiro Kotake, 2020/03, Without Invitation, Japanese
  45. Trehalose decreases Akt mRNA and protein expression, Kanae Umeda, Masatsugu Miyara, Shunichi Hatamiya, Yaichiro Kotake, 2020/03, Without Invitation, Japanese, Kyoto
  46. Reduced protein expression of lysosomal enzyme induced by 1BnTIQ, an endogenous Parkinson's disease-related neurotoxin, Ayaka Yabuki, Masatsugu Miyara, Kanae Umeda, Natsumi Okada, Namiko Watanabe, Shigeru Ohta, Yaichiro Kotake, 2020/03, Without Invitation, Japanese
  47. Endogenous parkinsonian neurotoxin 1BnTIQ impairs autophagic degradation, Miyara M, Yabuki A, Okada N, Umeda K, Nishio T, Yamada S, Kanda Y, Nakanishi T, Ohta S, Kotake Y, 2019/08, Without Invitation, English
  48. Inhibition of autophagy induced by 1BnTIQ, an endogenous Parkinson's disease-related neurotoxin, 2019/06, Without Invitation, Japanese
  49. Neurotoxic factors in Parkinson's disease., Ohta S, Kotake Y, Kohta R, Miyara M, Contu VR, Annual Meeting of the German Pharmaceutical Society, 2013/10, With Invitation, English, Freiburg
  50. Low concentration of Parkinson's disease-related neurotoxin MPP+ inhibits autophagy, Miyara M, Kotake Y, Ohta S, The XIII International Congress of Toxicology, 2013/07, Without Invitation, English, Seoul
  51. Inhibition of autophagic flux induced by low concentration of 1-methyl-4-phenylpyridinium ion, Miyara M, Kotake Y, Aoyama K, Ohta S, Society for Neuroscience 2012, 2012/10, Without Invitation, English, New Orleans
  52. Impairment of autophagy induced by low concentration of MPP+, a Parkinson's disease-related toxin, Miyara M, Kotake Y, Ohta S, The 6th International Symposium on Autophagy 2012, 2012/10, Without Invitation, English, Nago
  53. Involvement of autophagy in low concentration of MPP+-induced neuronal cell death, Miyara M, Kotake Y, Hirokane Y, Ohta S, The 6th International Congress of Asian Society of Toxicology, 2012/07, Without Invitation, English, Sendai

External Funds

Acceptance Results of Competitive Funds

  1. 2024/08/01, 2026/07/31
  2. 2024/04/01, 2025/03/31
  3. 2023/04/01, 2027/03/31
  4. KAKENHI(Grant-in-Aid for Early-Career Scientists), 2022/04/01, 2024/03/31
  5. 2020/04/01, 2024/03/31
  6. 2020/04, 2022/03
  7. 2018/04, 2021/03
  8. 2018/06, 2020/03
  9. 2017/12, 2019/03
  10. 2017/04, 2018/03
  11. 2017/03, 2018/02
  12. 2014/04, 2016/03