Last Updated :2024/07/05

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Pharmaceutical Sciences), Assistant Professor
Web Site
I investigate the mechanism underlying the toxicity of Parkinsonian neurotoxins (e.g., MPP+ and rotenone) by focusing on the function of organelles (e.g., lysosomes and mitochondria).

Basic Information

Major Professional Backgrounds

  • 2021/04/01, Hiroshima University, Graduate School of Biomedical and Health Sciences, Assistant Professor
  • 2018/04/01, 2021/03/31, Japan Society for the Promotion of Science, Research Fellow (PD)
  • 2017/04/01, 2018/03/31, Hiroshima University, Institute of Biomedical & Health Sciences, Postdoctoral Researcher
  • 2016/04/01, 2017/03/31, Hiroshima University, Special Postdoctoral Researcher
  • 2014/04/01, 2016/03/31, Japan Society for the Promotion of Science, Research Fellow (DC2)

Educational Backgrounds

  • Hiroshima University, 2012/04/01, 2016/03/23
  • Hiroshima University, 2010/04/01, 2012/03/23
  • Prefectural University of Hiroshima, 2006/04/01, 2010/03/19

Academic Degrees

  • Hiroshima University
  • Hiroshima University

Research Fields

  • Medicine,dentistry, and pharmacy;Pharmacy;Environmental and hygienic pharmacy
  • Medicine,dentistry, and pharmacy;Pharmacy;Biological pharmacy
  • Biology;Biological Science;Cell biology

Research Keywords

  • Parkinson's disease
  • Autophagy
  • Lysosome

Affiliated Academic Societies

  • The Pharmaceutical Society of Japan
  • The Japanese Society for Neurochemistry
  • The Japanese Society of Toxicology
  • The Molecular Biology Society of Japan

Educational Activity

Course in Charge

  1. 2024, Undergraduate Education, 2Term, Public Health Chemistry II
  2. 2024, Undergraduate Education, First Semester, Experiments of Public health Chemistry
  3. 2024, Liberal Arts Education Program1, 1Term, Introductory Seminar for First-Year Students

Research Activities

Academic Papers

  1. Photodegradation profiling of nitrendipine: evaluation of active pharmaceutical ingredient, tablets and its altered forms, ANALYTICAL SCIENCES, 39(11), 1791-1803, 202311
  2. Evaluation of photostability of azelnidipine tablets and structure determination of its photoproducts, JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 233, 20230905
  3. Tributyltin activates the Keapl-Nrf2 pathway via a macroautophagy-independent reduction in Keapl, JOURNAL OF TOXICOLOGICAL SCIENCES, 48(3), 161-168, 202303
  4. Methylmercury Decreases AMPA Receptor Subunit GluA2 Levels in Cultured Rat Cortical Neurons, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 46(2), 292-300, 202302
  5. ★, Trehalose decreases mRNA and protein expressions of c-Jun and JunB in human cervical cancer HeLa cells, JOURNAL OF BIOCHEMISTRY, 172(3), 177-187, 20220905
  6. ★, Tributyltin inhibits autophagy by decreasing lysosomal acidity in SH-SY5Y cells, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 592, 31-37, 20220212
  7. ★, MiT/TFE family members suppress L-leucyl-L-leucine methyl ester-induced cell death, The Journal of Toxicological Sciences, 46(3), 143-156, 2021
  8. Triphenyltin inhibits GA-binding protein α nuclear translocation, Fundamental Toxicological Sciences, 7(1), 33-40, 2020
  9. Carbofuran causes neuronal vulnerability to glutamate by decreasing GluA2 protein levels in rat primary cortical neurons, Archives of Toxicology, 92(1), 401-409, 201806
  10. Low-concentration tributyltin decreases GluR2 expression via nuclear respiratory factor-1 inhibition, International Journal of Molecular Sciences, 18(8), 1754, 20170811
  11. ★, Mild MPP+ exposure-induced glucose starvation enhances autophagosome synthesis and impairs its degradation, Scientific Reports, 7, 46668, 20170426
  12. Prenatal exposure to tributyltin decreases GluR2 expression in the mouse brain, Biological and Pharmaceutical Bulletin, 40(7), 1121-1124, 2017
  13. ★, Mild MPP+ exposure impairs autophagic degradation through a novel lysosomal acidity-independent mechanism, Journal of Neurochemistry, 139(2), 294-308, 201610
  14. Protein extracts from cultured cells contain nonspecific serum albumin, Bioscience, Biotechnology, and Biochemistry, 80(6), 1164-1167, 201606
  15. Methoxychlor and fenvalerate induce neuronal death by reducing GluR2 expression, The Journal of Toxicological Sciences, 41(2), 255-264, 201604
  16. Endogenous neurotoxic dopamine derivative covalently binds to Parkinson's disease-associated ubiquitin C-terminal hydrolase L1 and alters its structure and function, Journal of Neurochemistry, 130(6), 826-838, 201409
  17. Tributyltin-induced endoplasmic reticulum stress and its Ca(2+)-mediated mechanism, Toxicology and Applied Pharmacology, 272(1), 137-146, 201310
  18. Involvement of decreased glutamate receptor subunit GluR2 expression in lead-induced neuronal cell death, The Journal of Toxicological Sciences, 38(3), 513-521, 2013

Invited Lecture, Oral Presentation, Poster Presentation

  1. Identification of genes involved in the toxicity of Parkinson's disease-associated neurotoxin MPP+, 2024/03, Without Invitation, Japanese
  2. Cytotoxicity evaluation and synthetic utilization of trioctyltin compounds, 2024/03, Without Invitation, Japanese
  3. Low concentrations of MPP+ promote glucose metabolism and impair autophagy, 2023/09, With Invitation, Japanese
  4. Tributyltin induces LC3-II accumulation independent of autophagy initiation complex, 2023/09, Without Invitation, Japanese
  5. Discovery of p62 nuclear accumulation induced by a Parkinson's disease-related neurotoxin and a novel nuclear role of p62, 2023/09, Without Invitation, Japanese
  6. Investigation of the mechanism of insolubilization of intralysosomal hydrolases induced by the lysosomal membrane-damaging agent LLOMe, 2023/09, Without Invitation, Japanese
  7. Search for genes involvbed in susceptibility to Parkinson's disease-associated neurotoxin MPP+, 2023/09, Without Invitation, Japanese
  8. Exploring mRNA markers for assessing developmental neurotoxicity using primary rat neurons, Fujihara R, Miyara M, Takehara Y, Takabayashi T, Yamamoto Y, Tahara H, Kotake Y, 2023/09, Without Invitation, English
  9. Autophagy dysfunction and p62 nuclear accumulation induced by low concentrations of a parkinsonian neurotoxin, 2022/11, With Invitation, Japanese
  10. Exploration of nuclear degradation pathway of a lysosomal stress-responsive transcription factor TFEB, 2022/08, Without Invitation, Japanese
  11. Mechanism of tributyltin-induced LC3-II accumulation, 2022/08, Without Invitation, Japanese
  12. Investigation of the effects of parkinsonian neurotoxin 1BnTIQ on the autophagy-lysosome system, 2022/08, Without Invitation, Japanese
  13. Protein insolubilization induced by mitochondrial dysfunction during starvation, 2022/08, Without Invitation, Japanese
  14. TBT activates Keap1-Nrf2 pathway via Keap1 decrease, 2022/08, Without Invitation, Japanese
  15. Tributyltin accumulates LC3-II via lysosomal dysfunction, Hatamiya S, Miyara M, Kotake Y, 2022/08, Without Invitation, English, Kumamoto
  16. Tributyltin decreases lysosomal acidity and inhibits autophagic degradation, Hatamiya S, Miyara M, Kotake Y, The 8th International Symposium on Metallomics, 2022/07, Without Invitation, English, Kanazawa
  17. Parkinsonian neurotoxin 1BnTIQ inhibits autophagy via lysosomal impairment, 2022/03, Without Invitation, Japanese
  18. Changes in protein properties induced by mitochondrial dysfunction during starvation, 2022/03, Without Invitation, Japanese
  19. Tributyltin activates Nrf2 via Keap1 reduction, 2022/03, Without Invitation, Japanese
  20. Global transcriptome analysis in low concentration of MPP+-induced cellular model of Parkinson's disease, Natsumi Okada, Masatsugu Miyara, Kanae Miyara, Miyuki Kanda, Hidetoshi Tahara, Yaichiro Kotake, 2021/09, Without Invitation, Japanese
  21. Tributyltin inhibits autophagy via lysosomal dysfunction, Shunichi Hatamiya, Masatsugu Miyara, Yaichiro Kotake, 2021/07, Without Invitation, Japanese
  22. Mild parkinsonian neurotoxin MPP+ exposure disrupts lysosomal gene expression, Natsumi Okada, Masatsugu Miyara, Kanae Miyara, Shunichi Hatamiya, Yusuke Kojima, Saya Takao, Yaichiro Kotake, 2021/03, Without Invitation, Japanese
  23. Tributyltin impairs autophagy, Shunichi Hatamiya, Masatsugu Miyara, Yaichiro Kotake, 2021/03, Without Invitation, Japanese
  24. Mild parkinsonian neurotoxin exposure induces autophagy dysfunction and nuclear p62 accumulation, Masatsugu Miyara, Wataru Tokunaga, Yuka Kuwabara, Namiko Watanabe, Shunichi Hatamiya, Tsuyoshi Nakanishi, Yaichiro Kotake, 2020/03, Without Invitation, Japanese
  25. Trehalose decreases Akt mRNA and protein expression, Kanae Umeda, Masatsugu Miyara, Shunichi Hatamiya, Yaichiro Kotake, 2020/03, Without Invitation, Japanese, Kyoto
  26. Reduced protein expression of lysosomal enzyme induced by 1BnTIQ, an endogenous Parkinson's disease-related neurotoxin, Ayaka Yabuki, Masatsugu Miyara, Kanae Umeda, Natsumi Okada, Namiko Watanabe, Shigeru Ohta, Yaichiro Kotake, 2020/03, Without Invitation, Japanese
  27. Endogenous parkinsonian neurotoxin 1BnTIQ impairs autophagic degradation, Miyara M, Yabuki A, Okada N, Umeda K, Nishio T, Yamada S, Kanda Y, Nakanishi T, Ohta S, Kotake Y, 2019/08, Without Invitation, English
  28. Inhibition of autophagy induced by 1BnTIQ, an endogenous Parkinson's disease-related neurotoxin, 2019/06, Without Invitation, Japanese
  29. Neurotoxic factors in Parkinson's disease., Ohta S, Kotake Y, Kohta R, Miyara M, Contu VR, Annual Meeting of the German Pharmaceutical Society, 2013/10, With Invitation, English, Freiburg
  30. Low concentration of Parkinson's disease-related neurotoxin MPP+ inhibits autophagy, Miyara M, Kotake Y, Ohta S, The XIII International Congress of Toxicology, 2013/07, Without Invitation, English, Seoul
  31. Inhibition of autophagic flux induced by low concentration of 1-methyl-4-phenylpyridinium ion, Miyara M, Kotake Y, Aoyama K, Ohta S, Society for Neuroscience 2012, 2012/10, Without Invitation, English, New Orleans
  32. Impairment of autophagy induced by low concentration of MPP+, a Parkinson's disease-related toxin, Miyara M, Kotake Y, Ohta S, The 6th International Symposium on Autophagy 2012, 2012/10, Without Invitation, English, Nago
  33. Involvement of autophagy in low concentration of MPP+-induced neuronal cell death, Miyara M, Kotake Y, Hirokane Y, Ohta S, The 6th International Congress of Asian Society of Toxicology, 2012/07, Without Invitation, English, Sendai

External Funds

Acceptance Results of Competitive Funds

  1. 2024/04/01, 2025/03/31
  2. 2023/04/01, 2027/03/31
  3. KAKENHI(Grant-in-Aid for Early-Career Scientists), 2022/04/01, 2024/03/31
  4. 2020/04/01, 2024/03/31
  5. 2020/04, 2022/03
  6. 2018/04, 2021/03
  7. 2018/06, 2020/03
  8. 2017/12, 2019/03
  9. 2017/04, 2018/03
  10. 2017/03, 2018/02
  11. 2014/04, 2016/03