Keisuke Nakajima
Last Updated :2025/04/03
- Affiliations, Positions
- Amphibian Research Center, Assistant Professor
- E-mail
- kei
hiroshima-u.ac.jp
Basic Information
Major Professional Backgrounds
- 1993/04/01, 2001/08/31, Tokyo Metropolitan Institute for Neruoscience, Department of Molecular Neurobiology
- 2001/09/01, 2007/03/31, Hiroshima University, Insitute for Amphibian Biology, Graduate School of Science, Research Associate
Educational Backgrounds
- Waseda University, Graduate School, Division of Natural Science, Japan, 1991/04, 1993/03
- Waseda University, Faculty of Education, Japan, 1987/04, 1991/03
Academic Degrees
- Ph.D., Waseda University
- Master of Science, Waseda University
Research Fields
- Biological Sciences;Laboratory animal science;Laboratory animal science
Research Keywords
Educational Activity
Course in Charge
- 2025, Liberal Arts Education Program1, 3Term, The life science of amphibians
- 2025, Liberal Arts Education Program1, 2Term, Introductory Seminar for First-Year Students
- 2025, Undergraduate Education, Second Semester, English Seminar on Biological Science
- 2025, Undergraduate Education, 1Term, Regeneration Biology
- 2025, Undergraduate Education, 1Term, Endocrinology・Immunology
- 2025, Undergraduate Education, Second Semester, Seminar for Amphibian Biology
- 2025, Undergraduate Education, First Semester, Practice for Fundamental Biology I
- 2025, Undergraduate Education, First Semester, Practice for Fundamental Biology III
- 2025, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
- 2025, Undergraduate Education, First Semester, Practice for Fundamental Biology IV
- 2025, Undergraduate Education, 1Term, English Seminar for Globalization A
- 2025, Graduate Education (Master's Program) , 1Term, Seminar for Advanced Research in Basic Biology A
- 2025, Graduate Education (Master's Program) , 3Term, Seminar for Advanced Research in Basic Biology B
- 2025, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Basic Biology
- 2025, Graduate Education (Master's Program) , 1Term, Seminar for Advanced Research in Basic Biology C
- 2025, Graduate Education (Master's Program) , 3Term, Seminar for Advanced Research in Basic Biology D
- 2025, Graduate Education (Master's Program) , First Semester, Exercises in Basic Biology A
- 2025, Graduate Education (Master's Program) , Second Semester, Exercises in Basic Biology B
- 2025, Graduate Education (Doctoral Program) , 2Term, Seminar for Advanced Research in Basic Biology E
- 2025, Graduate Education (Doctoral Program) , 3Term, Seminar for Advanced Research in Basic Biology F
Research Activities
Academic Papers
- Generation of translucent Xenopus tropicalis through triple knockout of pigmentation genes, DEVELOPMENT GROWTH & DIFFERENTIATION, 65(9), 591-598, 202312
- Osteological and histological comparison of the development of the interphalangeal intercalary skeletal element between hyloid and ranoid anurans, DEVELOPMENT GROWTH & DIFFERENTIATION, 65(2), 100-108, 202302
- Intravital staining to detect mineralization in Xenopus tropicalis during and after metamorphosis, DEVELOPMENT GROWTH & DIFFERENTIATION, 64(7), 368-378, 202209
- Generation of no-yellow-pigment Xenopus tropicalis by slc2a7 gene knockout, DEVELOPMENTAL DYNAMICS, 250(10), 1420-1431, 202110
- Sperm MMP-2 is indispensable for fast electrical block to polyspermy at fertilization in Xenopus tropicalis, MOLECULAR REPRODUCTION AND DEVELOPMENT, 88(11), 744-757, 202111
- Comprehensive RNA-Seq analysis of notochord-enriched genes induced during Xenopus tropicalis tail resorption, GENERAL AND COMPARATIVE ENDOCRINOLOGY, 287, 20200201
- A unique role of thyroid hormone receptor beta in regulating notochord resorption during Xenopus metamorphosis, GENERAL AND COMPARATIVE ENDOCRINOLOGY, 277, 66-72, 20190601
- Xenopus Resources: Transgenic, Inbred and Mutant Animals, Training Opportunities, and Web-Based Support, FRONTIERS IN PHYSIOLOGY, 10, 2019
- ★, Thyroid Hormone Receptor alpha- and beta-Knockout Xenopus tropicalis Tadpoles Reveal Subtype-Specific Roles During Development, ENDOCRINOLOGY, 159(2), 733-743, 201802
- Developmental gene expression patterns in the brain and liver of Xenopus tropicalis during metamorphosis climax, GENES TO CELLS, 23(12), 998-1008, 201812
- An Inhibitor of Thyroid Hormone Synthesis Protects Tail Skin Grafts Transplanted to Syngenic Adult Frogs, ZOOLOGICAL SCIENCE, 34(5), 414-418, 201710
- no privacy, a Xenopus tropicalis mutant, is a model of human Hermansky-Pudlak Syndrome and allows visualization of internal organogenesis during tadpole development, DEVELOPMENTAL BIOLOGY, 426(2), 472-486, 20170615
- Mechanisms of tail resorption during anuran metamorphosis, Biomolecular Concepts, 8(3-4), 179-183, 2017
- Ouro proteins are not essential to tail regression during Xenopus tropicalis metamorphosis, GENES TO CELLS, 21(3), 275-286, 201603
- Generation of Albino Cynops pyrrhogaster by Genomic Editingof the tyrosinase Gene, ZOOLOGICAL SCIENCE, 33(3), 290-294, 201606
- ★, Highly efficient gene knockout by injection of TALEN mRNAs into oocytes and host transfer in Xenopus laevis, BIOLOGY OPEN, 4(2), 180-185, 20150215
- ★, Development of a new approach for targeted gene editing in primordial germ cells using TALENs in Xenopus, BIOLOGY OPEN, 4(3), 259-266, 20150315
- Xenopus pax6 mutants affect eye development and other organ systems, and have phenotypic similarities to human aniridia patients, Developmental Biology, 408(2), 328-344, 201512
- Expression of the amelogenin Gene in the Skin of Xenopus tropicalis, ZOOLOGICAL SCIENCE, 30(3), 154-159, 201303
- Targeted Gene Disruption in the Xenopus tropicalis Genome Using Designed TALE Nucleases, ZOOLOGICAL SCIENCE, 30(6), 455-460, 201306
- Comparison of TALEN scaffolds in Xenopus tropicalis, BIOLOGY OPEN, 2(12), 1364-1370, 20131215
- Translational regulation by the 5 '-UTR of thyroid hormone receptor alpha mRNA, JOURNAL OF BIOCHEMISTRY, 151(5), 519-531, 2012
- ★, Regulation of thyroid hormone sensitivity by differential expression of the thyroid hormone receptor during Xenopus metamorphosis, GENES TO CELLS, 17(8), 645-659, 201208
- Generation of albino Xenopus tropicalis using zinc-finger nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 54(9), 777-784, 201212
- Xenopus tropicalis: An Ideal Experimental Animal in Amphibia, EXPERIMENTAL ANIMALS, 59(4), 395-405, 201007
- Expression of matrix metalloproteinase genes in regressing or remodeling organs during amphibian metamorphosis, DEVELOPMENT GROWTH & DIFFERENTIATION, 49(2), 131-143, 2007
- One of the duplicated matrix metalloproteinase-9 genes is expressed in regressing tail during anuran metamorphosis, DEVELOPMENT GROWTH & DIFFERENTIATION, 48(4), 223-241, 200605
- Programmed cell death during amphibian metamorphosis, SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 16(2), 271-280, 200504
- Involvement of caspase-9 in execution of the maternal program of apoptosis in Xenopus late blastulae overexpressed with S-ademosylmethionine decarbosylase, Biochemical and Biophysical Research Communications, 325(4), 1367-1375, 20041201
- ★, Dual mechanisms governing muscle cell death in tadpole tail during amphibian metamorphosis, DEVELOPMENTAL DYNAMICS, 227(2), 246-255, 200306
- ★, Structure, Expression and Function of the Xenopus laevis Caspase Family, Journal of Biological Chemistry, 275(14), 10484-10491, 20000401
- Construction of multiple-epitope tag sequence by PCR for sensitive Western blot analysis, Nucleic Acids Research, 25(11), 2231-2232, 19970601
- Induction of apoptosis and CPP32 expression by thyroid hormone in a myoblastic cell line derived from tadpole tail, Journal of Biological Chemistry, 272(8), 5122-5127, 19970201
- Absence of Methylated Thyrotropin-Releasing Hormone in the Bullfrog (Rana catesbeiana) Brain, Zoological Science, 10, 711-714, 19931001
- Thyrotropin-releasing hormone (TRH) is the major prolactin-releasing factor in the bullfrog hypothalamus, General and Comparative Endocrinology, 89(1), 11-16, 19930101
- TALEN- and CRISPR/Cas9-mediated mutagenesis of pax6 and six3 genes for studying eye and brain development in Xenopus tropicalis, GENES & GENETIC SYSTEMS, 89(6), 283-283, 2014
- Gene Cloning and Characterization of the Xenopus Caspase Family, 21, 19981201
- Xenopus tropicalis: An Ideal Experimental Animal in Amphibia, Proceedings of The Japanese Society of Animal Models for Human Diseases, 59(4), 395-405, 2010
- MOLECULAR MECHANISM OF MUSCLE CELL DEATH IN A REGRESSING TAIL DURING AMPHIBIAN METAMORPHOSIS(Endocrinology,Abstracts of papers presented at the 74^
Annual Meeting of the Zoological Society of Japan), Zoological science, 20(12), 20031225- Comparison of TALEN scaffolds in Xenopus tropicalis., Comparison of TALEN scaffolds in Xenopus tropicalis., 2(12), 2013
Publications such as books- 2021, Diabetology, Endocrinology & Metabolology, The roles of two thyroid hormone receptors during amphibian metamorphosis, metamorphosis, thyroid hormone, thyroid hormone receptor, Xenopus tropicalis, knockout, 2021, Scholarly Book, Joint work, 日本語, Keisuke Nakajima, Ichiro Tazawa, Yoshio Yaoita, 53(5): 565-576
- 1998/10, 両生類の変態とアポトーシス , 医歯薬出版株式会社, 1998, 10, Scholarly Book, Cocompilation, 6
- 1996/11, 変態とアポトーシス , 羊土社, 1996, 11, Scholarly Book, Cocompilation, 2
Invited Lecture, Oral Presentation, Poster Presentation- Is there differences among thyroid hormone receptor alpha and beta?, 2024/09/14, Without Invitation, Japanese
- The evolutionary origin of arboreality in frogs by comparison of the development of digit skeletons., 2024/09/12, Without Invitation, Japanese
- The overlooked role of thyroxine in metamorphosis, Keisuke Nakajima, Souta Yabumoto, Ichiro Tazawa, Nobuaki Furuno, 2023/09/09, Without Invitation, Japanese
- Amphibian resource opens up cross-platform research, 2023/09/08, Without Invitation, Japanese
- Interaction between limb bud-derived tissues and the sacral rib during larval development of the newt Pleurodeles waltl, 2023/09/07, Without Invitation, Japanese
- Ectopic sacral ribs induction experiment in Pleurodeles waltl, 2022/09/08, Without Invitation, Japanese
- Expression analysis of olfm4 gene in Xenopus tropicalis and functional analysis using knockout frog, 2022/09/08, Without Invitation, Japanese
- Transformation during Amphibian Metamorphosis and Regeneration, Yoshio Yaoita*, Keisuke Nakajima and Ichiro Tazawa, International Symposium at Hiroshima University: Amphibian development, regeneration, evolution and beyond, 2018/03/13, Without Invitation, English
- Two unique TALEN methods for the highly efficient mutagenesis and genomic editing preferentially in germ cell using Xenopus, K. Nakajima and Y. Yaoita, K. Nakajima and Y. Yaoita, World Congress of Molecular & Cell Biology-2016, 2016/04, With Invitation, English, Dalian, China
- The role of the thyroid hormone receptor during Xenopus metamorphosis, Keisuke Nakajima, Keisuke Nakajima, Kenta Fujimoto and Yoshio Yaoita, 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology, 2014/11, With Invitation, English
- Xenopus tropicalis, a model organism for the new genetics era: from forward to reverse genetics and now to gene targeting, Takuya Nakayama, Takuya Nakayama, Margaret B. Fish, Marilyn Fisher, Keisuke Nakajima, Yoshio Yaoita and Robert M. Grainger, International Symposium Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing, 2014/03, With Invitation, English
- ネッタイツメガエルにおけるノックアウト法の活用, Keisuke Nakajima, Keisuke Nakajima, Yuya Nakai, Morihiro Okada, Minoru Takase, Yoshio Yaoita, 第83回日本動物学会, 2012, With Invitation, Japanese
- Low levels of Thyroid Hormone can Induce the Death of Thyroid Hormone Receptor-
Overexpressing Muscle Cells in Xenopus Tadpole Tail, Keisuke Nakajima, Nakajima, K., Fujimoto, K., and Yaoita, Y, 2009/12, With Invitation, English
- Expression of matrix metalloproteinase genes in regressing or remodeling organs during amphibian metamorphosis, Yoshio Yaoita, Kenta Fujimoto, Keisuke Nakajima and Yoshio Yaoita, 2007/03, With Invitation, English
- The regulation of thyroid hormone sensitivity of the Xenopus tadpole tail, Kenta Fujimoto, Kenta Fujimoto, Keisuke Nakajima, Yoshio Yaoita, 2006/05, With Invitation, English
- Molecular mechanism of tail muscle cell death in regressing tail during amphibian metamorphosis, Yoshio Yaoita, Yoshio Yaoita, Kenta Fujimoto and Keisuke Nakajima, NIH seminar, 2005/09, With Invitation, English
- The analysis of a myoblastic cell line derived from the amphibian tadpole tail., Yoshio Yaoita, Yoshio Yaoita, Keisuke Nakajima, 1999/07, With Invitation, English
Awards- 1999/03, Young scientist award, The Frontiers of the Biology of Amphibia, GENE CLONING AND CHARACTERIZATION OF THE XENOPUS LAEVIS CASPASE FAMILY
External FundsAcceptance Results of Competitive Funds- 2024, 2026
- KAKENHI(Grant-in-Aid for Scientific Research (C)), 2022, 2024
- KAKENHI(Grant-in-Aid for Scientific Research (C)), 2020, 2022
- 2019
- KAKENHI(Grant-in-Aid for Scientific Research (C)), 2019, 2021
- 2014, 2016
- KAKENHI, 2013, 2015
- KAKENHI, 2013, 2015
- KAKENHI, Mechanism of translational repression by uORF of thyroid hormone receptor a mRNA, 2009, 2011
- KAKENHI, The Molecular Mechanism of Organ Regression during the Development, 2002, 2004
- 2001
- 1999
- KAKENHI, 1999, 2000
- KAKENHI, Cloning of apoptosis-inducing genes by introducing genes into living tadpole, 1998, 2000
Social ActivitiesOrganizing Academic Conferences, etc.- 2022/09, 2022/09
- 2019/09, 2019/09
History as Peer Reviews of Academic Papers- 2024, Scientific Reports, Others, 2
- 2023, Zoological Science, Others, reviewer, 1
- 2020, Biology Open, Others, reviewer, 1
- 2020, Cytology & Tissue Biology, Others, reviewer, 1
- 2017, Cell & Bioscience, Others, reviewer, 1
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