Keisuke Nakajima

Last Updated :2021/11/25

Affiliations, Positions
Institute for Amphibian Biology, Assistant Professor
E-mail
keihiroshima-u.ac.jp

Basic Information

Major Professional Backgrounds

  • 1993/04/01, 2001/08/31, Tokyo Metropolitan Institute for Neruoscience, Department of Molecular Neurobiology
  • 2001/09/01, 2007/03/31, Hiroshima University, Insitute for Amphibian Biology, Graduate School of Science, Research Associate

Educational Backgrounds

  • Waseda University, Graduate School, Division of Natural Science, Japan, 1991/04, 1993/03
  • Waseda University, Faculty of Education, Japan, 1987/04, 1991/03

Academic Degrees

  • Ph.D., Waseda University
  • Master of Science, Waseda University

Research Fields

  • Biological Sciences;Laboratory animal science;Laboratory animal science

Research Keywords

  • muscle
  • Xenopus
  • apoptosis

Educational Activity

Course in Charge

  1. 2021, Liberal Arts Education Program1, 2Term, Introductory Seminar for First-Year Students
  2. 2021, Undergraduate Education, Second Semester, English Seminar on Biological Science
  3. 2021, Undergraduate Education, 1Term, Regeneration Biology
  4. 2021, Undergraduate Education, Second Semester, Seminar for Amphibian Biology
  5. 2021, Undergraduate Education, First Semester, Practice for Fundamental Biology I
  6. 2021, Undergraduate Education, First Semester, Practice for Fundamental Biology III
  7. 2021, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
  8. 2021, Undergraduate Education, 1Term, English Seminar for Globalization A
  9. 2021, Undergraduate Education, 2Term, English Seminar for Globalization A
  10. 2021, Graduate Education (Master's Program) , First Semester, Seminar for Biological Science (Lecture)
  11. 2021, Graduate Education (Master's Program) , Second Semester, Seminar for Biological Science (Lecture)
  12. 2021, Graduate Education (Master's Program) , 2Term, Seminar for Advanced Research in Basic Biology A
  13. 2021, Graduate Education (Master's Program) , 3Term, Seminar for Advanced Research in Basic Biology B
  14. 2021, Graduate Education (Master's Program) , 2Term, Seminar for Advanced Research in Basic Biology C
  15. 2021, Graduate Education (Master's Program) , 3Term, Seminar for Advanced Research in Basic Biology D
  16. 2021, Graduate Education (Doctoral Program) , 2Term, Seminar for Advanced Research in Basic Biology E
  17. 2021, Graduate Education (Doctoral Program) , 3Term, Seminar for Advanced Research in Basic Biology F
  18. 2021, Graduate Education (Master's Program) , 1Term, Exercises in Biomedical Science A
  19. 2021, Graduate Education (Master's Program) , 2Term, Exercises in Biomedical Science A
  20. 2021, Graduate Education (Master's Program) , 3Term, Exercises in Biomedical Science B
  21. 2021, Graduate Education (Master's Program) , 4Term, Exercises in Biomedical Science B

Research Activities

Academic Papers

  1. Translational regulation by the 5 '-UTR of thyroid hormone receptor alpha mRNA, JOURNAL OF BIOCHEMISTRY, 151(5), 519-531, 2012
  2. ★, Regulation of thyroid hormone sensitivity by differential expression of the thyroid hormone receptor during Xenopus metamorphosis, GENES TO CELLS, 17(8), 645-659, 201208
  3. Generation of albino Xenopus tropicalis using zinc-finger nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 54(9), 777-784, 201212
  4. Expression of the amelogenin Gene in the Skin of Xenopus tropicalis, ZOOLOGICAL SCIENCE, 30(3), 154-159, 201303
  5. Targeted Gene Disruption in the Xenopus tropicalis Genome Using Designed TALE Nucleases, ZOOLOGICAL SCIENCE, 30(6), 455-460, 201306
  6. Involvement of caspase-9 in execution of the maternal program of apoptosis in Xenopus late blastulae overexpressed with S-ademosylmethionine decarbosylase, Biochemical and Biophysical Research Communications, 325(4), 1367-1375, 20041201
  7. Absence of Methylated Thyrotropin-Releasing Hormone in the Bullfrog (Rana catesbeiana) Brain, Zoological Science, 10, 711-714, 19931001
  8. Thyrotropin-releasing hormone (TRH) is the major prolactin-releasing factor in the bullfrog hypothalamus, General and Comparative Endocrinology, 89(1), 11-16, 19930101
  9. ★, Structure, Expression and Function of the Xenopus laevis Caspase Family, Journal of Biological Chemistry, 275(14), 10484-10491, 20000401
  10. Construction of multiple-epitope tag sequence by PCR for sensitive Western blot analysis, Nucleic Acids Research, 25(11), 2231-2232, 19970601
  11. Induction of apoptosis and CPP32 expression by thyroid hormone in a myoblastic cell line derived from tadpole tail, Journal of Biological Chemistry, 272(8), 5122-5127, 19970201
  12. ★, Dual mechanisms governing muscle cell death in tadpole tail during amphibian metamorphosis, DEVELOPMENTAL DYNAMICS, 227(2), 246-255, 200306
  13. Programmed cell death during amphibian metamorphosis, SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 16(2), 271-280, 200504
  14. One of the duplicated matrix metalloproteinase-9 genes is expressed in regressing tail during anuran metamorphosis, DEVELOPMENT GROWTH & DIFFERENTIATION, 48(4), 223-241, 200605
  15. Expression of matrix metalloproteinase genes in regressing or remodeling organs during amphibian metamorphosis, DEVELOPMENT GROWTH & DIFFERENTIATION, 49(2), 131-143, 2007
  16. Xenopus tropicalis: An Ideal Experimental Animal in Amphibia, EXPERIMENTAL ANIMALS, 59(4), 395-405, 201007
  17. ★, Highly efficient gene knockout by injection of TALEN mRNAs into oocytes and host transfer in Xenopus laevis, BIOLOGY OPEN, 4(2), 180-185, 20150215
  18. ★, Development of a new approach for targeted gene editing in primordial germ cells using TALENs in Xenopus, BIOLOGY OPEN, 4(3), 259-266, 20150315
  19. Comparison of TALEN scaffolds in Xenopus tropicalis, BIOLOGY OPEN, 2(12), 1364-1370, 20131215
  20. TALEN- and CRISPR/Cas9-mediated mutagenesis of pax6 and six3 genes for studying eye and brain development in Xenopus tropicalis, GENES & GENETIC SYSTEMS, 89(6), 283-283, 2014
  21. Ouro proteins are not essential to tail regression during Xenopus tropicalis metamorphosis, GENES TO CELLS, 21(3), 275-286, 201603
  22. Generation of Albino Cynops pyrrhogaster by Genomic Editingof the tyrosinase Gene, ZOOLOGICAL SCIENCE, 33(3), 290-294, 201606
  23. Gene Cloning and Characterization of the Xenopus Caspase Family, 21, 19981201
  24. Xenopus tropicalis: An Ideal Experimental Animal in Amphibia, Proceedings of The Japanese Society of Animal Models for Human Diseases, 59(4), 395-405, 2010
  25. Xenopus pax6 mutants affect eye development and other organ systems, and have phenotypic similarities to human aniridia patients, Developmental Biology, 408(2), 328-344, 201512
  26. An Inhibitor of Thyroid Hormone Synthesis Protects Tail Skin Grafts Transplanted to Syngenic Adult Frogs, ZOOLOGICAL SCIENCE, 34(5), 414-418, 201710
  27. ★, Thyroid Hormone Receptor alpha- and beta-Knockout Xenopus tropicalis Tadpoles Reveal Subtype-Specific Roles During Development, ENDOCRINOLOGY, 159(2), 733-743, 201802
  28. no privacy, a Xenopus tropicalis mutant, is a model of human Hermansky-Pudlak Syndrome and allows visualization of internal organogenesis during tadpole development, DEVELOPMENTAL BIOLOGY, 426(2), 472-486, 20170615
  29. Mechanisms of tail resorption during anuran metamorphosis, Biomolecular Concepts, 8(3-4), 179-183, 2017
  30. Developmental gene expression patterns in the brain and liver of Xenopus tropicalis during metamorphosis climax, GENES TO CELLS, 23(12), 998-1008, 201812
  31. A unique role of thyroid hormone receptor beta in regulating notochord resorption during Xenopus metamorphosis, GENERAL AND COMPARATIVE ENDOCRINOLOGY, 277, 66-72, 20190601
  32. MOLECULAR MECHANISM OF MUSCLE CELL DEATH IN A REGRESSING TAIL DURING AMPHIBIAN METAMORPHOSIS(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan), Zoological science, 20(12), 20031225
  33. Comparison of TALEN scaffolds in Xenopus tropicalis., Comparison of TALEN scaffolds in Xenopus tropicalis., 2(12), 2013
  34. Comprehensive RNA-Seq analysis of notochord-enriched genes induced during Xenopus tropicalis tail resorption, GENERAL AND COMPARATIVE ENDOCRINOLOGY, 287, 20200201
  35. Xenopus Resources: Transgenic, Inbred and Mutant Animals, Training Opportunities, and Web-Based Support, FRONTIERS IN PHYSIOLOGY, 10, 2019

Publications such as books

  1. 1998/10, 両生類の変態とアポトーシス , 医歯薬出版株式会社, 1998, 10, Scholarly Book, Cocompilation, 6
  2. 1996/11, 変態とアポトーシス , 羊土社, 1996, 11, Scholarly Book, Cocompilation, 2

Invited Lecture, Oral Presentation, Poster Presentation

  1. Two unique TALEN methods for the highly efficient mutagenesis and genomic editing preferentially in germ cell using Xenopus, K. Nakajima and Y. Yaoita, K. Nakajima and Y. Yaoita, World Congress of Molecular & Cell Biology-2016, 2016/04, With Invitation, English, Dalian, China
  2. The role of the thyroid hormone receptor during Xenopus metamorphosis, Keisuke Nakajima, Keisuke Nakajima, Kenta Fujimoto and Yoshio Yaoita, 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology, 2014/11, With Invitation, English
  3. Xenopus tropicalis, a model organism for the new genetics era: from forward to reverse genetics and now to gene targeting, Takuya Nakayama, Takuya Nakayama, Margaret B. Fish, Marilyn Fisher, Keisuke Nakajima, Yoshio Yaoita and Robert M. Grainger, International Symposium Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing, 2014/03, With Invitation, English
  4. ネッタイツメガエルにおけるノックアウト法の活用, Keisuke Nakajima, Keisuke Nakajima, Yuya Nakai, Morihiro Okada, Minoru Takase, Yoshio Yaoita, 第83回日本動物学会, 2012, With Invitation, Japanese
  5. Low levels of Thyroid Hormone can Induce the Death of Thyroid Hormone Receptor- Overexpressing Muscle Cells in Xenopus Tadpole Tail, Keisuke Nakajima, Nakajima, K., Fujimoto, K., and Yaoita, Y, 2009/12, With Invitation, English
  6. Expression of matrix metalloproteinase genes in regressing or remodeling organs during amphibian metamorphosis, Yoshio Yaoita, Kenta Fujimoto, Keisuke Nakajima and Yoshio Yaoita, 2007/03, With Invitation, English
  7. The regulation of thyroid hormone sensitivity of the Xenopus tadpole tail, Kenta Fujimoto, Kenta Fujimoto, Keisuke Nakajima, Yoshio Yaoita, 2006/05, With Invitation, English
  8. Molecular mechanism of tail muscle cell death in regressing tail during amphibian metamorphosis, Yoshio Yaoita, Yoshio Yaoita, Kenta Fujimoto and Keisuke Nakajima, NIH seminar, 2005/09, With Invitation, English
  9. The analysis of a myoblastic cell line derived from the amphibian tadpole tail., Yoshio Yaoita, Yoshio Yaoita, Keisuke Nakajima, 1999/07, With Invitation, English

Awards

  1. 1999/03, Young scientist award, The Frontiers of the Biology of Amphibia, GENE CLONING AND CHARACTERIZATION OF THE XENOPUS LAEVIS CASPASE FAMILY

External Funds

Acceptance Results of Competitive Funds

  1. KAKENHI(Grant-in-Aid for Scientific Research (C)), 2020, 2022
  2. 2019
  3. KAKENHI(Grant-in-Aid for Scientific Research (C)), 2019, 2021
  4. 2014, 2016
  5. KAKENHI, 2013, 2015
  6. KAKENHI, 2013, 2015
  7. KAKENHI, Mechanism of translational repression by uORF of thyroid hormone receptor a mRNA, 2009, 2011
  8. KAKENHI, The Molecular Mechanism of Organ Regression during the Development, 2002, 2004
  9. 2001
  10. 1999
  11. KAKENHI, 1999, 2000
  12. KAKENHI, Cloning of apoptosis-inducing genes by introducing genes into living tadpole, 1998, 2000