Keisuke Nakajima

Last Updated :2025/04/03

Affiliations, Positions
Amphibian Research Center, Assistant Professor
E-mail
keihiroshima-u.ac.jp

Basic Information

Major Professional Backgrounds

  • 1993/04/01, 2001/08/31, Tokyo Metropolitan Institute for Neruoscience, Department of Molecular Neurobiology
  • 2001/09/01, 2007/03/31, Hiroshima University, Insitute for Amphibian Biology, Graduate School of Science, Research Associate

Educational Backgrounds

  • Waseda University, Graduate School, Division of Natural Science, Japan, 1991/04, 1993/03
  • Waseda University, Faculty of Education, Japan, 1987/04, 1991/03

Academic Degrees

  • Ph.D., Waseda University
  • Master of Science, Waseda University

Research Fields

  • Biological Sciences;Laboratory animal science;Laboratory animal science

Research Keywords

  • muscle
  • Xenopus
  • apoptosis

Educational Activity

Course in Charge

  1. 2025, Liberal Arts Education Program1, 3Term, The life science of amphibians
  2. 2025, Liberal Arts Education Program1, 2Term, Introductory Seminar for First-Year Students
  3. 2025, Undergraduate Education, Second Semester, English Seminar on Biological Science
  4. 2025, Undergraduate Education, 1Term, Regeneration Biology
  5. 2025, Undergraduate Education, 1Term, Endocrinology・Immunology
  6. 2025, Undergraduate Education, Second Semester, Seminar for Amphibian Biology
  7. 2025, Undergraduate Education, First Semester, Practice for Fundamental Biology I
  8. 2025, Undergraduate Education, First Semester, Practice for Fundamental Biology III
  9. 2025, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
  10. 2025, Undergraduate Education, First Semester, Practice for Fundamental Biology IV
  11. 2025, Undergraduate Education, 1Term, English Seminar for Globalization A
  12. 2025, Graduate Education (Master's Program) , 1Term, Seminar for Advanced Research in Basic Biology A
  13. 2025, Graduate Education (Master's Program) , 3Term, Seminar for Advanced Research in Basic Biology B
  14. 2025, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Basic Biology
  15. 2025, Graduate Education (Master's Program) , 1Term, Seminar for Advanced Research in Basic Biology C
  16. 2025, Graduate Education (Master's Program) , 3Term, Seminar for Advanced Research in Basic Biology D
  17. 2025, Graduate Education (Master's Program) , First Semester, Exercises in Basic Biology A
  18. 2025, Graduate Education (Master's Program) , Second Semester, Exercises in Basic Biology B
  19. 2025, Graduate Education (Doctoral Program) , 2Term, Seminar for Advanced Research in Basic Biology E
  20. 2025, Graduate Education (Doctoral Program) , 3Term, Seminar for Advanced Research in Basic Biology F

Research Activities

Academic Papers

  1. Generation of translucent Xenopus tropicalis through triple knockout of pigmentation genes, DEVELOPMENT GROWTH & DIFFERENTIATION, 65(9), 591-598, 202312
    Link to Paper Search Results by DOI
  2. Osteological and histological comparison of the development of the interphalangeal intercalary skeletal element between hyloid and ranoid anurans, DEVELOPMENT GROWTH & DIFFERENTIATION, 65(2), 100-108, 202302
    Link to Paper Search Results by DOI
  3. Intravital staining to detect mineralization in Xenopus tropicalis during and after metamorphosis, DEVELOPMENT GROWTH & DIFFERENTIATION, 64(7), 368-378, 202209
    Link to Paper Search Results by DOI
  4. Generation of no-yellow-pigment Xenopus tropicalis by slc2a7 gene knockout, DEVELOPMENTAL DYNAMICS, 250(10), 1420-1431, 202110
    Link to Paper Search Results by DOI
  5. Sperm MMP-2 is indispensable for fast electrical block to polyspermy at fertilization in Xenopus tropicalis, MOLECULAR REPRODUCTION AND DEVELOPMENT, 88(11), 744-757, 202111
    Link to Paper Search Results by DOI
  6. Comprehensive RNA-Seq analysis of notochord-enriched genes induced during Xenopus tropicalis tail resorption, GENERAL AND COMPARATIVE ENDOCRINOLOGY, 287, 20200201
    Link to Paper Search Results by DOI
  7. A unique role of thyroid hormone receptor beta in regulating notochord resorption during Xenopus metamorphosis, GENERAL AND COMPARATIVE ENDOCRINOLOGY, 277, 66-72, 20190601
    Link to Paper Search Results by DOI
  8. Xenopus Resources: Transgenic, Inbred and Mutant Animals, Training Opportunities, and Web-Based Support, FRONTIERS IN PHYSIOLOGY, 10, 2019
  9. ★, Thyroid Hormone Receptor alpha- and beta-Knockout Xenopus tropicalis Tadpoles Reveal Subtype-Specific Roles During Development, ENDOCRINOLOGY, 159(2), 733-743, 201802
    Link to Paper Search Results by DOI
  10. Developmental gene expression patterns in the brain and liver of Xenopus tropicalis during metamorphosis climax, GENES TO CELLS, 23(12), 998-1008, 201812
    Link to Paper Search Results by DOI
  11. An Inhibitor of Thyroid Hormone Synthesis Protects Tail Skin Grafts Transplanted to Syngenic Adult Frogs, ZOOLOGICAL SCIENCE, 34(5), 414-418, 201710
    Link to Paper Search Results by DOI
  12. no privacy, a Xenopus tropicalis mutant, is a model of human Hermansky-Pudlak Syndrome and allows visualization of internal organogenesis during tadpole development, DEVELOPMENTAL BIOLOGY, 426(2), 472-486, 20170615
    Link to Paper Search Results by DOI
  13. Mechanisms of tail resorption during anuran metamorphosis, Biomolecular Concepts, 8(3-4), 179-183, 2017
  14. Ouro proteins are not essential to tail regression during Xenopus tropicalis metamorphosis, GENES TO CELLS, 21(3), 275-286, 201603
    Link to Paper Search Results by DOI
  15. Generation of Albino Cynops pyrrhogaster by Genomic Editingof the tyrosinase Gene, ZOOLOGICAL SCIENCE, 33(3), 290-294, 201606
    Link to Paper Search Results by DOI
  16. ★, Highly efficient gene knockout by injection of TALEN mRNAs into oocytes and host transfer in Xenopus laevis, BIOLOGY OPEN, 4(2), 180-185, 20150215
    Link to Paper Search Results by DOI
  17. ★, Development of a new approach for targeted gene editing in primordial germ cells using TALENs in Xenopus, BIOLOGY OPEN, 4(3), 259-266, 20150315
    Link to Paper Search Results by DOI
  18. Xenopus pax6 mutants affect eye development and other organ systems, and have phenotypic similarities to human aniridia patients, Developmental Biology, 408(2), 328-344, 201512
  19. Expression of the amelogenin Gene in the Skin of Xenopus tropicalis, ZOOLOGICAL SCIENCE, 30(3), 154-159, 201303
    Link to Paper Search Results by DOI
  20. Targeted Gene Disruption in the Xenopus tropicalis Genome Using Designed TALE Nucleases, ZOOLOGICAL SCIENCE, 30(6), 455-460, 201306
    Link to Paper Search Results by DOI
  21. Comparison of TALEN scaffolds in Xenopus tropicalis, BIOLOGY OPEN, 2(12), 1364-1370, 20131215
    Link to Paper Search Results by DOI
  22. Translational regulation by the 5 '-UTR of thyroid hormone receptor alpha mRNA, JOURNAL OF BIOCHEMISTRY, 151(5), 519-531, 2012
    Link to Paper Search Results by DOI
  23. ★, Regulation of thyroid hormone sensitivity by differential expression of the thyroid hormone receptor during Xenopus metamorphosis, GENES TO CELLS, 17(8), 645-659, 201208
    Link to Paper Search Results by DOI
  24. Generation of albino Xenopus tropicalis using zinc-finger nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 54(9), 777-784, 201212
    Link to Paper Search Results by DOI
  25. Xenopus tropicalis: An Ideal Experimental Animal in Amphibia, EXPERIMENTAL ANIMALS, 59(4), 395-405, 201007
    Link to Paper Search Results by DOI
  26. Expression of matrix metalloproteinase genes in regressing or remodeling organs during amphibian metamorphosis, DEVELOPMENT GROWTH & DIFFERENTIATION, 49(2), 131-143, 2007
    Link to Paper Search Results by DOI
  27. One of the duplicated matrix metalloproteinase-9 genes is expressed in regressing tail during anuran metamorphosis, DEVELOPMENT GROWTH & DIFFERENTIATION, 48(4), 223-241, 200605
    Link to Paper Search Results by DOI
  28. Programmed cell death during amphibian metamorphosis, SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 16(2), 271-280, 200504
    Link to Paper Search Results by DOI
  29. Involvement of caspase-9 in execution of the maternal program of apoptosis in Xenopus late blastulae overexpressed with S-ademosylmethionine decarbosylase, Biochemical and Biophysical Research Communications, 325(4), 1367-1375, 20041201
  30. ★, Dual mechanisms governing muscle cell death in tadpole tail during amphibian metamorphosis, DEVELOPMENTAL DYNAMICS, 227(2), 246-255, 200306
    Link to Paper Search Results by DOI
  31. ★, Structure, Expression and Function of the Xenopus laevis Caspase Family, Journal of Biological Chemistry, 275(14), 10484-10491, 20000401
  32. Construction of multiple-epitope tag sequence by PCR for sensitive Western blot analysis, Nucleic Acids Research, 25(11), 2231-2232, 19970601
  33. Induction of apoptosis and CPP32 expression by thyroid hormone in a myoblastic cell line derived from tadpole tail, Journal of Biological Chemistry, 272(8), 5122-5127, 19970201
  34. Absence of Methylated Thyrotropin-Releasing Hormone in the Bullfrog (Rana catesbeiana) Brain, Zoological Science, 10, 711-714, 19931001
  35. Thyrotropin-releasing hormone (TRH) is the major prolactin-releasing factor in the bullfrog hypothalamus, General and Comparative Endocrinology, 89(1), 11-16, 19930101
  36. TALEN- and CRISPR/Cas9-mediated mutagenesis of pax6 and six3 genes for studying eye and brain development in Xenopus tropicalis, GENES & GENETIC SYSTEMS, 89(6), 283-283, 2014
  37. Gene Cloning and Characterization of the Xenopus Caspase Family, 21, 19981201
  38. Xenopus tropicalis: An Ideal Experimental Animal in Amphibia, Proceedings of The Japanese Society of Animal Models for Human Diseases, 59(4), 395-405, 2010
  39. MOLECULAR MECHANISM OF MUSCLE CELL DEATH IN A REGRESSING TAIL DURING AMPHIBIAN METAMORPHOSIS(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan), Zoological science, 20(12), 20031225
  40. Comparison of TALEN scaffolds in Xenopus tropicalis., Comparison of TALEN scaffolds in Xenopus tropicalis., 2(12), 2013

Publications such as books

  1. 2021, Diabetology, Endocrinology & Metabolology, The roles of two thyroid hormone receptors during amphibian metamorphosis, metamorphosis, thyroid hormone, thyroid hormone receptor, Xenopus tropicalis, knockout, 2021, Scholarly Book, Joint work, 日本語, Keisuke Nakajima, Ichiro Tazawa, Yoshio Yaoita, 53(5): 565-576
  2. 1998/10, 両生類の変態とアポトーシス , 医歯薬出版株式会社, 1998, 10, Scholarly Book, Cocompilation, 6
  3. 1996/11, 変態とアポトーシス , 羊土社, 1996, 11, Scholarly Book, Cocompilation, 2

Invited Lecture, Oral Presentation, Poster Presentation

  1. Is there differences among thyroid hormone receptor alpha and beta?, 2024/09/14, Without Invitation, Japanese
  2. The evolutionary origin of arboreality in frogs by comparison of the development of digit skeletons., 2024/09/12, Without Invitation, Japanese
  3. The overlooked role of thyroxine in metamorphosis, Keisuke Nakajima, Souta Yabumoto, Ichiro Tazawa, Nobuaki Furuno, 2023/09/09, Without Invitation, Japanese
  4. Amphibian resource opens up cross-platform research, 2023/09/08, Without Invitation, Japanese
  5. Interaction between limb bud-derived tissues and the sacral rib during larval development of the newt Pleurodeles waltl, 2023/09/07, Without Invitation, Japanese
  6. Ectopic sacral ribs induction experiment in Pleurodeles waltl, 2022/09/08, Without Invitation, Japanese
  7. Expression analysis of olfm4 gene in Xenopus tropicalis and functional analysis using knockout frog, 2022/09/08, Without Invitation, Japanese
  8. Transformation during Amphibian Metamorphosis and Regeneration, Yoshio Yaoita*, Keisuke Nakajima and Ichiro Tazawa, International Symposium at Hiroshima University: Amphibian development, regeneration, evolution and beyond, 2018/03/13, Without Invitation, English
  9. Two unique TALEN methods for the highly efficient mutagenesis and genomic editing preferentially in germ cell using Xenopus, K. Nakajima and Y. Yaoita, K. Nakajima and Y. Yaoita, World Congress of Molecular & Cell Biology-2016, 2016/04, With Invitation, English, Dalian, China
  10. The role of the thyroid hormone receptor during Xenopus metamorphosis, Keisuke Nakajima, Keisuke Nakajima, Kenta Fujimoto and Yoshio Yaoita, 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology, 2014/11, With Invitation, English
  11. Xenopus tropicalis, a model organism for the new genetics era: from forward to reverse genetics and now to gene targeting, Takuya Nakayama, Takuya Nakayama, Margaret B. Fish, Marilyn Fisher, Keisuke Nakajima, Yoshio Yaoita and Robert M. Grainger, International Symposium Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing, 2014/03, With Invitation, English
  12. ネッタイツメガエルにおけるノックアウト法の活用, Keisuke Nakajima, Keisuke Nakajima, Yuya Nakai, Morihiro Okada, Minoru Takase, Yoshio Yaoita, 第83回日本動物学会, 2012, With Invitation, Japanese
  13. Low levels of Thyroid Hormone can Induce the Death of Thyroid Hormone Receptor- Overexpressing Muscle Cells in Xenopus Tadpole Tail, Keisuke Nakajima, Nakajima, K., Fujimoto, K., and Yaoita, Y, 2009/12, With Invitation, English
  14. Expression of matrix metalloproteinase genes in regressing or remodeling organs during amphibian metamorphosis, Yoshio Yaoita, Kenta Fujimoto, Keisuke Nakajima and Yoshio Yaoita, 2007/03, With Invitation, English
  15. The regulation of thyroid hormone sensitivity of the Xenopus tadpole tail, Kenta Fujimoto, Kenta Fujimoto, Keisuke Nakajima, Yoshio Yaoita, 2006/05, With Invitation, English
  16. Molecular mechanism of tail muscle cell death in regressing tail during amphibian metamorphosis, Yoshio Yaoita, Yoshio Yaoita, Kenta Fujimoto and Keisuke Nakajima, NIH seminar, 2005/09, With Invitation, English
  17. The analysis of a myoblastic cell line derived from the amphibian tadpole tail., Yoshio Yaoita, Yoshio Yaoita, Keisuke Nakajima, 1999/07, With Invitation, English

Awards

  1. 1999/03, Young scientist award, The Frontiers of the Biology of Amphibia, GENE CLONING AND CHARACTERIZATION OF THE XENOPUS LAEVIS CASPASE FAMILY

External Funds

Acceptance Results of Competitive Funds

  1. 2024, 2026
  2. KAKENHI(Grant-in-Aid for Scientific Research (C)), 2022, 2024
  3. KAKENHI(Grant-in-Aid for Scientific Research (C)), 2020, 2022
  4. 2019
  5. KAKENHI(Grant-in-Aid for Scientific Research (C)), 2019, 2021
  6. 2014, 2016
  7. KAKENHI, 2013, 2015
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  8. KAKENHI, 2013, 2015
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  9. KAKENHI, Mechanism of translational repression by uORF of thyroid hormone receptor a mRNA, 2009, 2011
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  10. KAKENHI, The Molecular Mechanism of Organ Regression during the Development, 2002, 2004
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  11. 2001
  12. 1999
  13. KAKENHI, 1999, 2000
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  14. KAKENHI, Cloning of apoptosis-inducing genes by introducing genes into living tadpole, 1998, 2000
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Social Activities

Organizing Academic Conferences, etc.

  1. 2022/09, 2022/09
  2. 2019/09, 2019/09

History as Peer Reviews of Academic Papers

  1. 2024, Scientific Reports, Others, 2
  2. 2023, Zoological Science, Others, reviewer, 1
  3. 2020, Biology Open, Others, reviewer, 1
  4. 2020, Cytology & Tissue Biology, Others, reviewer, 1
  5. 2017, Cell & Bioscience, Others, reviewer, 1