TOMOHARU YASUDA

Last Updated :2022/11/02

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Medicine), Professor
E-mail
yasudathiroshima-u.ac.jp
Self-introduction
Yasuda's laboratory studies how immune system is regulated through the antigen receptor, intracellular signaling and lymphocyte programming. We hope our research make breakthrough to overcome difficult diseases such as immunodeficiency, allergy, autoimmunity and cancer.

Basic Information

Major Professional Backgrounds

  • 2000/07/01, 2001/04/30, Japan Society for the Promotion of Science, Research Fellowship for Young Scientists (DC2)
  • 2001/05/01, 2005/03/31, Medical Research Institute, Tokyo Medical and Dental University, Assistant Professor
  • 2005/04/01, 2008/11/30, Research Center for Allergy and Immunology, RIKEN, Research Fellow
  • 2008/12/01, 2011/07/31, Immune Disease Institute, Harvard Medical School, Reserch fellow
  • 2009/04/01, 2011/03/31, Japan Society for the Promotion of Science, JSPS Postdoctoral Fellow for Research Abroad
  • 2011/08/01, 2017/01/31, Max Delbruck Center for Molecular Medicine, Scientist
  • 2017/02/01, 2017/03/31, National Center for Geriatrics and Gerontology, Postdoc
  • 2017/04/01, 2019/07/31, Medical Institute of Bioregulation, Kyushu University, Associate Professor
  • 2019/08/01, Graduate School of Biomedical and Health Sciences, Hiroshima University, Department of Immunology, Professor

Educational Backgrounds

  • University of Tokyo, Graduate School of Medicine, Doctoral Course, Japan, 1998/04/01, 2001/04/30
  • Kyushu University, Graduate School of Agriculture, Department of Genetic Resources Technology, Master's Course, Japan, 1996/04/01, 1998/03/31
  • Kyushu University, Faculty of Agriculture, Japan, 1992/04/01, 1996/03/31

Academic Degrees

  • Ph.D., The University of Tokyo
  • M.Sc., Kyushu University

Educational Activity

  • [Bachelor Degree Program] School of Medicine : Program of Medicine : Medicine
  • [Master's Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Biomedical Science
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Biomedical Science
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Biomedical Sciences : Program of Medicine

In Charge of Primary Major Programs

  • Medicine

Research Fields

  • Medicine,dentistry, and pharmacy;Basic medicine;Immunology
  • Medicine,dentistry, and pharmacy;Basic medicine;Parasitology (including sanitary zoology)

Research Keywords

  • Antigen receptor, Development of lymphocytes, Immunodeficiency, Lymphoma, Immune surveillance, Gene therapy, Allergy, Autoimmune disease

Affiliated Academic Societies

  • Japanese Society for Immunology
  • Japanese Cancer Association
  • The Molecular Biology Society of Japan
  • Japanese Association for Laboratory Animal Science

Educational Activity

Course in Charge

  1. 2022, Undergraduate Education, Year, Biological Responses
  2. 2022, Undergraduate Education, 2Term, Introduction for medical research
  3. 2022, Undergraduate Education, Intensive, Practice for medical research
  4. 2022, Undergraduate Education, 1Term, Microbiology Immunology
  5. 2022, Graduate Education (Master's Program) , First Semester, Seminar
  6. 2022, Graduate Education (Master's Program) , Second Semester, Seminar
  7. 2022, Graduate Education (Master's Program) , First Semester, Research
  8. 2022, Graduate Education (Master's Program) , Second Semester, Research
  9. 2022, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar
  10. 2022, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar
  11. 2022, Graduate Education (Doctoral Program) , First Semester, Advanced Research
  12. 2022, Graduate Education (Doctoral Program) , Second Semester, Advanced Research
  13. 2022, Graduate Education (Master's Program) , First Semester, Physiology and Biological Chemistry
  14. 2022, Graduate Education (Master's Program) , 3Term, Seminar on Host Defense
  15. 2022, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Immunology
  16. 2022, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Immunology
  17. 2022, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Immunology
  18. 2022, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Immunology
  19. 2022, Graduate Education (Doctoral Program) , 2Term, Trends of Genome Editing in Medical Science

Research Activities

Academic Papers

  1. Efficient Isolation of Lymphocytes and Myogenic Cells from the Tissue of Muscle Regeneration, CELLS, 11(11), 202206
  2. Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis, NATURE COMMUNICATIONS, 13(1), 20220228
  3. Potential role of inducible GPR3 expression under stimulated T cell conditions, JOURNAL OF PHARMACOLOGICAL SCIENCES, 148(3), 307-314, 202203
  4. A ubiquitin-like protein encoded by the "noncoding" RNA TINCR promotes keratinocyte proliferation and wound healing, PLOS GENETICS, 17(8), 202108
  5. Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells, SCIENTIFIC REPORTS, 11(1), 20210309
  6. Pathologically Relevant Mouse Models for Epstein-Barr Virus-Associated B Cell Lymphoma, FRONTIERS IN IMMUNOLOGY, 12, 20210224
  7. A conserved PI(4,5)P2-binding domain is critical for immune regulatory function of DOCK8, LIFE SCIENCE ALLIANCE, 4(4), 202104
  8. Functional Interplay of Epstein-Barr virus oncoproteins in a mouse model of B cell lymphomagenesis., Proc. Natl. Acad. Sci. U. S. A., 117, 14421-14432, 2020
  9. Measuring protein synthesis during cell cycle by Azidohomoalanine (AHA) labeling and flow cytometric analysis., Bio-Protocol, 9, 2019
  10. Phosphorylation of the Ribosomal Protein RPL12/uL11 Affects Translation during Mitosis., Molecular cell, 72(1), 2018
  11. ★, Mouse model for acute Epstein-Barr virus infection., Proceedings of the National Academy of Sciences of the United States of America, 113(48), 2016
  12. Protein kinase D regulates positive selection of CD4+ thymocytes through phosphorylation of SHP-1., Nature communications, 7, 2016
  13. TAK1 adaptor proteins, TAB2 and TAB3, link the signalosome to B-cell receptor-induced IKK activation., FEBS letters, 590(18), 2016
  14. Canonical NF-kB signaling is uniquely required for the long-term persistence of functional mature B cells., Proceedings of the National Academy of Sciences of the United States of America, 113(18), 2016
  15. miR-23∼27∼24 clusters control effector T cell differentiation and function., The Journal of experimental medicine, 213(2), 2016
  16. PI3 Kinase and FOXO1 Transcription Factor Activity Differentially Control B Cells in the Germinal Center Light and Dark Zones., Immunity, 43(6), 2015
  17. RC3H1 post-transcriptionally regulates A20 mRNA and modulates the activity of the IKK/NF-kB pathway., Nature communications, 6, 2015
  18. Positive feedback within a kinase signaling complex functions as a switch mechanism for NF-kB activation., Science (New York, N.Y.), 344(6185), 2014
  19. An ITAM-Syk-CARD9 signalling axis triggers contact hypersensitivity by stimulating IL-1 production in dendritic cells., Nature communications, 5, 2014
  20. ★, Studying Epstein-Barr virus pathologies and immune surveillance by reconstructing EBV infection in mice., Cold Spring Harbor symposia on quantitative biology, 78, 2013
  21. ★, Immune surveillance and therapy of lymphomas driven by Epstein-Barr virus protein LMP1 in a mouse model., Cell, 148(4), 2012
  22. ★, ERKs induce expression of the transcriptional repressor Blimp-1 and subsequent plasma cell differentiation., Science signaling, 4(169), 2011
  23. Regulation of microRNA expression and abundance during lymphopoiesis., Immunity, 32(6), 2010
  24. ★, Regulation of lymphocyte fate by Ras/ERK signals., Cell cycle (Georgetown, Tex.), 7(23), 2008
  25. ★, Erk kinases link pre-B cell receptor signaling to transcriptional events required for early B cell expansion., Immunity, 28(4), 2008
  26. Dok1 mediates high-fat diet-induced adipocyte hypertrophy and obesity through modulation of PPAR-gamma phosphorylation., Nature medicine, 14(2), 2008
  27. Dok-1 is a positive regulator of IL-4 signalling and IgE response., Journal of biochemistry, 142(2), 2007
  28. Dok-1 and Dok-2 are negative regulators of T cell receptor signaling., International immunology, 19(4), 2007
  29. The adaptor-like protein ROG-1 is required for activation of the Ras-MAP kinase pathway and meiotic cell cycle progression in Caenorhabditis elegans., Genes to cells : devoted to molecular & cellular mechanisms, 12(3), 2007
  30. Dok-3 sequesters Grb2 and inhibits the Ras-Erk pathway downstream of protein-tyrosine kinases., Genes to cells : devoted to molecular & cellular mechanisms, 11(2), 2006
  31. PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1., The Journal of experimental medicine, 202(10), 2005
  32. Dok-1 and Dok-2 are negative regulators of lipopolysaccharide-induced signaling., The Journal of experimental medicine, 201(3), 2005
  33. Functional impairment of telomerase in sublines derived from human lung adenocarcinoma exposed to mild oxidative stress., Biochemical and biophysical research communications, 334(2), 2005
  34. ★, Role of Dok-1 and Dok-2 in myeloid homeostasis and suppression of leukemia., The Journal of experimental medicine, 200(12), 2004
  35. Dok-1 tyrosine residues at 336 and 340 are essential for the negative regulation of Ras-Erk signalling, but dispensable for rasGAP-binding., Genes to cells : devoted to molecular & cellular mechanisms, 9(6), 2004
  36. ★, Cbl-b positively regulates Btk-mediated activation of phospholipase C-gamma2 in B cells., The Journal of experimental medicine, 196(1), 2002
  37. BANK regulates BCR-induced calcium mobilization by promoting tyrosine phosphorylation of IP(3) receptor., The EMBO journal, 21(1-2), 2002
  38. ★, Cbl suppresses B cell receptor-mediated phospholipase C (PLC)-g2 activation by regulating B cell linker protein-PLC-g2 binding., J. Exp. Med., 191, 641-650, 2000
  39. Bidirectional regulation of telomerase activity in a subline derived from human lung adenocarcinoma., Biochem. Biophys. Res. Commun., 237, 313-317, 1997

Publications such as books

  1. 2015, B Cell Receptor Signaling (ed, Kurosaki, T, and Wienands, J.). Current Topics in Microbiology and Immunology, Mitogen-activated protein kinases (MAPKs) play roles in a cell type and context-dependent manner to convert extracellular stimuli to a variety of cellular responses, thereby directing cells to proliferation, differentiation, survival, apoptosis, and migration. Studies of genetically engineered mice or chemical inhibitors specific to each MAPK signaling pathway revealed that MAPKs have various, but non-redundant physiologically important roles among different families. MAPK cascades are obviously integrated in the B cell receptor signaling pathways as critical components to drive B cell-mediated immunity., MAP Kinase Cascades in Antigen Receptor Signaling and Physiology, Springer, 2015, Scholarly Book, Joint work, English, Yasuda, T.
  2. 2004, B Cell Protocols (ed, Gu, H, and Rajewsky, K.). Methods Mol. Biol., A chicken DT40 B-cell line provides an excellent model system for the analysis of the function of genes related to B-cell antigen receptor (BCR) signaling. Crosslinking of BCR by micro-chain-specific antibody stimulates DT40 cells to undergo apoptosis. Activation of protein- tyrosine kinases (PTKs) and intracellular calcium signaling are essential for BCR-induced apoptosis. Because DT40 B cells are highly proliferative and readily integrate exogenous DNA by homologous recombination, gene targeting is conveniently employed to inactivate genes of interest. This chapter describes procedures for (1) generation of knockout DT40 cell line, (2) analysis of surface BCR expression, (3) detection of tyrosine-phosphorylated proteins, (4) detection of intracellular calcium mobilization, and (5) reconstitution of depleted gene by transfection., Analysis of B-cell signaling using DT40 B-cell line, Humana Press, 2004, Scholarly Book, Joint work, English, Yasuda, T., and Yamamoto, T.

Invited Lecture, Oral Presentation, Poster Presentation

  1. Efficient CRISPR/Cas9-mediated precise gene repair in T cells to treat EBV-triggered primary HLH in mice and human, Efficient CRISPR/Cas9-mediated precise gene repair in T cells to treat EBV-triggered primary HLH in mice and human, 2022/06/29, Without Invitation, English
  2. B cell division in the heterogeneous pre-tumor microenvironment is required for lymphoma development., T. Yasuda, K. Rajewsky, The 79th Annual Meeting of the Japanese Cancer Association, 2020/10/01, Without Invitation, English
  3. EB virus LMP1-mediated host immunogenicity and cytotoxic T cell differentiation, T. Yasuda, 2019/09/14, With Invitation, English
  4. Mouse gene targeting using NEPA21 electroporator, T. Yasuda, Japanese Association for Laboratory Animal Science, 2019/05/15, With Invitation, Japanese

External Funds

Acceptance Results of Competitive Funds

  1. KAKENHI(Grant-in-Aid for Scientific Research (B)), 2021, 2023
  2. KAKENHI(Grant-in-Aid for Challenging Research (Exploratory)), 2019, 2020
  3. KAKENHI(Grant-in-Aid for Challenging Research (Exploratory)), 2018, 2020

Social Activities

History as Peer Reviews of Academic Papers

  1. 2019, Frontiers in Microbiology, Editor, Editor, 1