KAZUHISA OUHARA

Last Updated :2025/05/09

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Dentistry & Oral Health Sciences), Assistant Professor
E-mail
kouharahiroshima-u.ac.jp
Self-introduction
Periodontal disease (PD) is a chronic infection in which the tissues around the teeth (gingiva, periodontal ligament, alveolar bone) are destroyed by periodontopathogenic bacteria, causing tooth loss. However, it may be associated with various systemic diseases (diabetes, liver disease, kidney disease, rheumatoid arthritis, heart disease, Alzheimer's disease, aspiration pneumonia, premature birth, low birth weight infant birth) as well as local in the oral cavity. It has been clarified in epidemiological studies and basic studies. Our research group has elucidated and reported on the relationship between diabetes, rheumatoid arthritis, liver disease, heart disease, preterm birth, Alzheimer's disease and periodontitis.

Basic Information

Academic Degrees

  • Doctor of Philosophy in Dental Science, Hiroshima University

Educational Activity

  • [Bachelor Degree Program] School of Dentistry : Program of Dentistry : Dentistry
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Biomedical Sciences : Program of Dental Sciences

Research Fields

  • Medicine,dentistry, and pharmacy;Dentistry;Periodontology

Research Keywords

  • periodontitis, immune response

Educational Activity

Course in Charge

  1. 2025, Liberal Arts Education Program1, 2Term, General Health and Oral Sciences I
  2. 2025, Undergraduate Education, First Semester, Early Clinical Exposure I
  3. 2025, Undergraduate Education, 3Term, Periodontology I
  4. 2025, Undergraduate Education, Second Semester, Early Clinical Exposure II
  5. 2025, Undergraduate Education, 1Term, Basic Practice of Periodontics I
  6. 2025, Undergraduate Education, 2Term, Basic Practice of Periodontics II
  7. 2025, Undergraduate Education, 2Term, Lifestyle-related Dentistry and Geriatric Dentistry
  8. 2025, Undergraduate Education, First Semester, Dental Research Practices I
  9. 2025, Undergraduate Education, Second Semester, Early Clinical Exposure III
  10. 2025, Undergraduate Education, Second Semester, Dental Research Practices II
  11. 2025, Undergraduate Education, 2Term, Advanced Course in Applied Oral Biology and Medicine
  12. 2025, Undergraduate Education, 2Term, General Seminar on Clinical Dentistry
  13. 2025, Undergraduate Education, First Semester, Dental Research Practices III
  14. 2025, Undergraduate Education, Year, Clinical Practice (Periodontics)
  15. 2025, Undergraduate Education, 2Term, Lifestyle-related Dentistry and Geriatric Dentistry
  16. 2025, Undergraduate Education, Second Semester, Clinical Practice on Oral Health
  17. 2025, Undergraduate Education, Intensive, Clinical Practice on Oral Health
  18. 2025, Undergraduate Education, Intensive, Clinical Practice on Oral Health Management
  19. 2025, Undergraduate Education, 2Term, Lifestyle-related Dentistry and Geriatric Dentistry

Research Activities

Academic Papers

  1. The Lipopolysaccharide Mutant Re-LPS Is a Useful Tool for Detecting LPS Contamination in Rheumatoid Synovial Cell Cultures, PATHOBIOLOGY, 89(2), 92-100, 20220322
    Link to Paper Search Results by DOI
  2. Periostin regulates integrin expression in gingival epithelial cells, JOURNAL OF ORAL BIOSCIENCES, 66(1), 170-178, 202403
    Link to Paper Search Results by DOI
  3. Loss-of-function OGFRL1 variants identified in autosomal recessive cherubism families, JBMR PLUS, 8(6), 20240430
    Link to Paper Search Results by DOI
  4. Mechanosignaling YAP/TAZ-TEAD Axis Regulates the Immunomodulatory Properties of Mesenchymal Stem Cells, STEM CELL REVIEWS AND REPORTS, 20(1), 347-361, 202401
    Link to Paper Search Results by DOI
  5. ★, N-glycan in the variable region of monoclonal ACPA (CCP-Ab1) promotes the exacerbation of experimental arthritis, RHEUMATOLOGY, 62(12), 3968-3977, 20231201
    Link to Paper Search Results by DOI
  6. Distinctive Biological Properties between Mesenchymal Stem Cell Spheroids and Clumps of Mesenchymal Stem Cells/Extracellular Matrix Complexes in 3D Culture Systems, APPLIED SCIENCES-BASEL, 13(23), 202312
    Link to Paper Search Results by DOI
  7. ★, Relationship Between Periodontitis and Atrial Fibrosis in Atrial Fibrillation Histological Evaluation of Left Atrial Appendages, JACC-CLINICAL ELECTROPHYSIOLOGY, 9(1), 43-53, 202301
    Link to Paper Search Results by DOI
  8. A Cartilaginous Construct with Bone Collar Exerts Bone-Regenerative Property Via Rapid Endochondral Ossification, STEM CELL REVIEWS AND REPORTS, 19(6), 1812-1827, 202308
    Link to Paper Search Results by DOI
  9. In Situ Raman Analysis of Biofilm Exopolysaccharides Formed in Streptococcus mutans and Streptococcus sanguinis Commensal Cultures, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24(7), 202304
    Link to Paper Search Results by DOI
  10. Extracellular Release of Citrullinated Vimentin Directly Acts on Osteoclasts to Promote Bone Resorption in a Mouse Model of Periodontitis, CELLS, 12(8), 202304
    Link to Paper Search Results by DOI
  11. Regulation of osteogenesis in bone marrow-derived mesenchymal stem cells via histone deacetylase 1 and 2 co-cultured with human gingival fibroblasts and periodontal ligament cells, JOURNAL OF PERIODONTAL RESEARCH, 58(1), 83-96, 202302
    Link to Paper Search Results by DOI
  12. Periapical lesion following Cnm-positive Streptococcus mutans pulp infection worsens cerebral hemorrhage onset in an SHRSP rat model, CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 210(3), 321-330, 20221231
    Link to Paper Search Results by DOI
  13. ★, Anti-Inflammatory Effects of Geniposidic Acid on Porphyromonas gingivalis-Induced Periodontitis in Mice, BIOMEDICINES, 10(12), 202212
    Link to Paper Search Results by DOI
  14. Clumps of mesenchymal stem cells/extracellular matrix complexes directly reconstruct the functional periodontal tissue in a rat periodontal defect model, JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 16(10), 945-955, 202210
    Link to Paper Search Results by DOI
  15. Masticatory dysfunction in patients with diabetic neuropathy: A cross-sectional study, PLOS ONE, 17(6), 2022
    Link to Paper Search Results by DOI
  16. Xenotransplantation of cryopreserved human clumps of mesenchymal stem cells/extracellular matrix complexes pretreated with IFN-? induces rat calvarial bone regeneration, REGENERATIVE THERAPY, 20, 117-125, 202206
    Link to Paper Search Results by DOI
  17. Cancellous bone-like tissue replacement from calcinosis in patients with systemic sclerosis with multiple external root resorption, BONE REPORTS, 16, 202206
    Link to Paper Search Results by DOI
  18. Comprehensive characterization of sortase A-dependent surface proteins in Streptococcus mutans, MICROBIOLOGY AND IMMUNOLOGY, 66(3), 145-156, 202203
    Link to Paper Search Results by DOI
  19. Involvement of Rac1 in macrophage activation by brain-derived neurotrophic factor, MOLECULAR BIOLOGY REPORTS, 48(6), 5249-5257, 202106
    Link to Paper Search Results by DOI
  20. External cervical resorption detected via cone-beam computed tomography in a patient with myelin oligodendrocyte glycoprotein antibody-associated disease: A case report, CLINICAL CASE REPORTS, 9(7), 202107
    Link to Paper Search Results by DOI
  21. The role of nuclear receptor 4A1 (NR4A1) in drug-induced gingival overgrowth, FASEB JOURNAL, 35(7), 202107
    Link to Paper Search Results by DOI
  22. Periodontitis and the outcome of atrial fibrillation ablation: Porphyromonas gingivalis is related to atrial fibrillation recurrence, JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY, 32(5), 1240-1250, 202105
    Link to Paper Search Results by DOI
  23. Selective and easy detection of the Porphyromonas gingivalis fimA type II and IV genes by loop-mediated isothermal amplification, JOURNAL OF MICROBIOLOGICAL METHODS, 185, 202106
    Link to Paper Search Results by DOI
  24. Functional Regulatory Mechanisms Underlying Bone Marrow Mesenchymal Stem Cell Senescence During Cell Passages, CELL BIOCHEMISTRY AND BIOPHYSICS, 79(2), 321-336, 202106
    Link to Paper Search Results by DOI
  25. Physiologic Target, Molecular Evolution, and Pathogenic Functions of a Monoclonal Anti-Citrullinated Protein Antibody Obtained From a Patient With Rheumatoid Arthritis, ARTHRITIS & RHEUMATOLOGY, 72(12), 2040-2049, 202012
    Link to Paper Search Results by DOI
  26. IL-6 Induced by Periodontal Inflammation Causes Neuroinflammation and Disrupts the Blood-Brain Barrier, BRAIN SCIENCES, 10(10), 202010
    Link to Paper Search Results by DOI
  27. Effect ofPorphyromonas gingivalisinfection on gut dysbiosis and resultant arthritis exacerbation in mouse model, ARTHRITIS RESEARCH & THERAPY, 22(1), 20201019
    Link to Paper Search Results by DOI
  28. Tuning cytokines enriches dendritic cells and regulatory T cells in the periodontium, JOURNAL OF PERIODONTOLOGY, 91(11), 1475-1485, 202011
    Link to Paper Search Results by DOI
  29. Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification, ANALYTICAL BIOCHEMISTRY, 605, 20200915
    Link to Paper Search Results by DOI
  30. Aggressive periodontitis and NOD2 variants, JOURNAL OF HUMAN GENETICS, 65(10), 841-846, 202010
    Link to Paper Search Results by DOI
  31. Cox2-mediated PGE2 production via p38/JNK-c-fos signaling inhibits cell apoptosis in 3D floating culture clumps of mesenchymal stem cell/extracellular matrix complexes, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 530(2), 448-454, 20200917
    Link to Paper Search Results by DOI
  32. Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification, Analytical Biochemistry, 20200915
  33. Physiological target, molecular evolution and pathogenic functions of a monoclonal ACPA obtained from an RA patient, Arthritis and Rheumatology, in press(in press), in press-in press, 20200704
  34. Regulation of osteogenesis via miR-101-3p in mesenchymal stem cells by human gingival fibroblasts, JOURNAL OF BONE AND MINERAL METABOLISM, 38(4), 442-455, 202007
    Link to Paper Search Results by DOI
  35. Optineurin regulates osteoblastogenesis through STAT1, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 525(4), 889-894, 20200514
    Link to Paper Search Results by DOI
  36. Multiple External Root Resorption of Teeth as a New Manifestation of Systemic Sclerosis-A Cross-Sectional Study in Japan, JOURNAL OF CLINICAL MEDICINE, 8(10), 201910
    Link to Paper Search Results by DOI
  37. Relationship between periodontal inflammation and calcium channel blockers induced gingival overgrowth-a cross-sectional study in a Japanese population, CLINICAL ORAL INVESTIGATIONS, 23(11), 4099-4105, 201911
    Link to Paper Search Results by DOI
  38. Clumps of Mesenchymal Stem Cell/Extracellular Matrix Complexes Generated with Xeno-Free Conditions Facilitate Bone Regeneration via Direct and Indirect Osteogenesis, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(16), 20190802
    Link to Paper Search Results by DOI
  39. Glycyrrhizic acid suppresses inflammation and reduces the increased glucose levels induced by the combination of Porphyromonas gulae and ligature placement in diabetic model mice, INTERNATIONAL IMMUNOPHARMACOLOGY, 68, 30-38, 201903
    Link to Paper Search Results by DOI
  40. Type I collagen deposition via osteoinduction ameliorates YAP/TAZ activity in 3D floating culture clumps of mesenchymal stem cell/extracellular matrix complexes, STEM CELL RESEARCH & THERAPY, 9, 20181207
    Link to Paper Search Results by DOI
  41. BDNF/HMW-HA complex as an adjunct to nonsurgical periodontal treatment of ligature-induced periodontitis in dogs, JOURNAL OF PERIODONTOLOGY, 90(1), 98-109, 201901
    Link to Paper Search Results by DOI
  42. The involvement of C5a in the progression of experimental arthritis with Porphyromonas gingivalis infection in SKG mice, ARTHRITIS RESEARCH & THERAPY, 20, 20181103
    Link to Paper Search Results by DOI
  43. Reparative bone-like tissue formation in the tooth of a systemic sclerosis patient, INTERNATIONAL ENDODONTIC JOURNAL, 51(9), 1059-1066, 201809
    Link to Paper Search Results by DOI
  44. Regulation of defensive function on gingival epithelial cells can prevent periodontal disease, JAPANESE DENTAL SCIENCE REVIEW, 54(2), 66-75, 201805
    Link to Paper Search Results by DOI
  45. The induced RNA-binding protein, HuR, targets 3-UTR region of IL-6 mRNA and enhances its stabilization in periodontitis, CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 192(3), 325-336, 201806
    Link to Paper Search Results by DOI
  46. Retrospective Case Series Analysis to Evaluate Ridge Augmentation Procedure Applied to Immediate Implant Placement in the Esthetic Zone: Five-Year Longitudinal Evaluation Using Cone Beam Computed Tomography, INTERNATIONAL JOURNAL OF PERIODONTICS & RESTORATIVE DENTISTRY, 37(4), 521-530, 2017
    Link to Paper Search Results by DOI
  47. Cryopreserved clumps of mesenchymal stem cell/extracellular matrix complexes retain osteogenic capacity and induce bone regeneration, STEM CELL RESEARCH & THERAPY, 9, 20180321
    Link to Paper Search Results by DOI
  48. MSC/ECM Cellular Complexes Induce Periodontal Tissue Regeneration, JOURNAL OF DENTAL RESEARCH, 96(9), 984-991, 201708
    Link to Paper Search Results by DOI
  49. Xenotransplantation of interferon-gamma-pretreated clumps of a human mesenchymal stem cell/extracellular matrix complex induces mouse calvarial bone regeneration, STEM CELL RESEARCH & THERAPY, 8, 20170426
    Link to Paper Search Results by DOI
  50. Brain-Derived Neurotrophic Factor Inhibits Peptidoglycan-Induced Inflammatory Cytokine Expression in Human Dental Pulp Cells, INFLAMMATION, 40(1), 240-247, 201702
    Link to Paper Search Results by DOI
  51. Mobilization of TLR4 Into Lipid Rafts by Aggregatibacter Actinomycetemcomitans in Gingival Epithelial Cells, CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 39(5), 1777-1786, 2016
    Link to Paper Search Results by DOI
  52. Aggregatibacter actinomycetemcomitans outer membrane protein 29 (Omp29) induces TGF--regulated apoptosis signal in human gingival epithelial cells via fibronectin/integrin1/FAK cascade, CELLULAR MICROBIOLOGY, 18(12), 1723-1738, 201612
    Link to Paper Search Results by DOI
  53. Porphyromonas gingivalis infection exacerbates the onset of rheumatoid arthritis in SKG mice, CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 186(2), 177-189, 201611
    Link to Paper Search Results by DOI
  54. Azithromycin recovers reductions in barrier function in human gingival epithelial cells stimulated with tumor necrosis factor-alpha, ARCHIVES OF ORAL BIOLOGY, 62, 64-69, 201602
    Link to Paper Search Results by DOI
  55. Distinction Between Cell Proliferation and Apoptosis Signals Regulated by Brain-Derived Neurotrophic Factor in Human Periodontal Ligament Cells and Gingival Epithelial Cells, JOURNAL OF CELLULAR BIOCHEMISTRY, 117(7), 1543-1555, 201607
    Link to Paper Search Results by DOI
  56. Involvement of smad2 and Erk/Akt cascade in TGF-beta 1-induced apoptosis in human gingival epithelial cells, CYTOKINE, 75(1), 165-173, 201509
    Link to Paper Search Results by DOI
  57. Irsogladine maleate inhibits Porphyromonas gingivalis-mediated expression of toll-like receptor 2 and interleukin-8 in human gingival epithelial cells, JOURNAL OF PERIODONTAL RESEARCH, 50(4), 486-493, 201508
    Link to Paper Search Results by DOI
  58. LL37 induces VEGF expression in dental pulp cells through ERK signalling, INTERNATIONAL ENDODONTIC JOURNAL, 48(7), 673-679, 201507
    Link to Paper Search Results by DOI
  59. The differential expression of mgl mRNA by Porphyromonas gingivalis affects the production of methyl mercaptan, ORAL DISEASES, 21(5), 626-633, 201507
    Link to Paper Search Results by DOI
  60. Clumps of a mesenchymal stromal cell/extracellular matrix complex can be a novel tissue engineering therapy for bone regeneration, CYTOTHERAPY, 17(7), 860-873, 201507
    Link to Paper Search Results by DOI
  61. Houttuynia cordata suppresses the Aggregatibacter actinomycetemcomitans-induced increase of inflammatory-related genes in cultured human gingival epithelial cells, JOURNAL OF DENTAL SCIENCES, 10(1), 88-94, 201503
    Link to Paper Search Results by DOI
  62. Brain-derived neurotrophic factor stimulates bone/cementum-related protein gene expression in cementoblasts, The Journal of Biological Chemistry, 283(23), 16259-16267, 20080601
  63. Bacteria-derived hydrogen sulfide promotes IL-8 production from epithelial cells., Biochem Biophys Res Commun., 20100101
  64. Hydrogen from intestinal bacteria is protective for Concanavalin A-induced hepatitis., Biochem Biophys Res Commun., 20090801
  65. Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate., Biochem Biophys Res Commun., 20090801
  66. The antimicrobial peptide LL37 induces the migration of human pulp cells: a possible adjunct for regenerative endodontics., Journal of Endodontology, 36(6), 1006-1013, 20100601
  67. Irsogladine maleate regulates neutrophil migration and E-cadherin expression in gingival epithelium stimulated by Aggregatibacter actinomycetemcomitans., Biochemical Pharmacology, 15(79)(10), 1496-1505, 20100501
  68. Induction of keratinocyte migration via transactivation of the epidermal growth factor receptor by the antimicrobial peptide LL-37, JOURNAL OF IMMUNOLOGY, 175(7), 4662-4668, 20051001
    Link to Paper Search Results by DOI
  69. Actinobacillus actinomycetemcomitans outer membrane protein 100 triggers innate immunity and production of beta-defensin and the 18-kilodalton cationic antimicrobial protein through the fibronectin-integrin pathway in human gingival epithelial cells, INFECTION AND IMMUNITY, 74(9), 5211-5220, 200609
    Link to Paper Search Results by DOI
  70. Irsogladine maleate regulates neutrophil migration and E-cadherin expression in gingival epithelium stimulated by Aggregatibacter actinomycetemcomitans, BIOCHEMICAL PHARMACOLOGY, 79(10), 1496-1505, 20100515
    Link to Paper Search Results by DOI
  71. Irsogladine maleate regulates epithelial barrier function in tumor necrosis factor-alpha-stimulated human gingival epithelial cells, JOURNAL OF PERIODONTAL RESEARCH, 47(1), 55-61, 201202
    Link to Paper Search Results by DOI
  72. Smad2 is involved in the apoptosis of murine gingival junctional epithelium associated with inhibition of Bcl-2, ARCHIVES OF ORAL BIOLOGY, 57(11), 1567-1573, 201211
    Link to Paper Search Results by DOI
  73. Antimicrobial peptide LL37 promotes vascular endothelial growth factor-A expression in human periodontal ligament cells, JOURNAL OF PERIODONTAL RESEARCH, 48(2), 228-234, 201304
    Link to Paper Search Results by DOI
  74. Irsogladine maleate regulates the inflammatory related genes in human gingival epithelial cells stimulated by Aggregatibacter actinomycetemcomitans, INTERNATIONAL IMMUNOPHARMACOLOGY, 15(2), 340-347, 201302
    Link to Paper Search Results by DOI
  75. The antimicrobial peptide LL37 promotes bone regeneration in a rat calvarial bone defect, PEPTIDES, 46, 136-142, 201308
    Link to Paper Search Results by DOI
  76. Dental infection of Porphyromonas gingivalis exacerbates high fat diet-induced steatohepatitis in mice, JOURNAL OF GASTROENTEROLOGY, 48(11), 1259-1270, 201311
    Link to Paper Search Results by DOI
  77. BDNF mimetic compound LM22A-4 regulates cementoblast differentiation via the TrkB-ERK/Akt signaling cascade, INTERNATIONAL IMMUNOPHARMACOLOGY, 19(2), 245-252, 201404
    Link to Paper Search Results by DOI
  78. ★, miR-584 Expressed in Human Gingival Epithelial Cells Is Induced by Porphyromonas gingivalis Stimulation and Regulates Interleukin-8 Production via Lactoferrin Receptor, JOURNAL OF PERIODONTOLOGY, 85(6), E198-E204, 201406
    Link to Paper Search Results by DOI
  79. Amphotericin B down-regulates Aggregatibacter actinomycetemcomitans-induced production of IL-8 and IL-6 in human gingival epithelial cells, CELLULAR IMMUNOLOGY, 290(2), 201-208, 201408
    Link to Paper Search Results by DOI
  80. Smad2 is Involved in Aggregatibacter actinomycetemcomitans-induced Apoptosis, JOURNAL OF DENTAL RESEARCH, 93(11), 1148-1154, 201411
    Link to Paper Search Results by DOI
  81. Clumps of Mesenchymal Stem Cells/Extracellular Matrix Complexes Generated with Xeno-Free Chondro-Inductive Medium Induce Bone Regeneration via Endochondral Ossification, BIOMEDICINES, 9(10), 202110
    Link to Paper Search Results by DOI

Invited Lecture, Oral Presentation, Poster Presentation

  1. Rheumatoid arthritis affected by periodontogenic bacterium, Porphyromonas gingivalis and SDGs, Kazuhisa Ouhara, Th 76th Annual Meeting of Japanese Stomatological Sciety, 2022/04/22, With Invitation, Japanese, Fukuoka City, experimental
  2. The pathology of periodontal disease and its effect on systemic diseases, Kazuhisa Ouhara, 2022/01/19, With Invitation, Japanese, other
  3. The effect of periodontopathogenic bacteriam, Porphyromonas gingivalis, against systemic diseases, Kazuhisa Ouhara, 48th Journal of Germfree Life and Gnotobiology, 2015/01/31, With Invitation, Japanese, Journal of Germfree Life and Gnotobiology, hiroshima
  4. The molecular analysis of Rheumatoid Arthritis-related gene LRG1 in periodontitis, Kazuhisa Ouhara, Syuichi Munenaga, Masahiro Yamakawa, Mikihito Kajiya, Satoshi Yamasaki, Tsuyoshi Fujita, Hideki Shiba, Eiji Sugiyama, Hidemi Kurihara, 93rd General Session & Exhibition of the IADR, 2015/03/11, Without Invitation, English, International Association for Dental Research, Boston, USA
  5. Porphyromonas gingivalis infection exacerbates rheumatoid arthritis in experimental mice model., Masahiro Yamakawa, Kazuhisa Ouhara, Mikihito Kajiya, Mizuho Kittaka, Katsuhiro Takeda, Takako Fujita, Tsuyoshi Fujita, Hideki Shiba and Hidemi Kurihara, 93rd General Session & Exhibition of the IADR, 2015/03/11, Without Invitation, English, International Association for Dental Research, Boston, USA
  6. The effect of periodontopathogenic bacteria, Porphyromonas gingivalis, against the onset of rheumatoid arthritis, Kazuhisa Ouhara, Masahiro Yamakawa, Syuichi Munenaga, Tsuyoshi Fujita and Hidemi Kurihara, 6th Hiroshima Conference on Education and Science in Dentistry, 2015/10/25, With Invitation, English, Hiroshima University, Hiroshima, Periodontitis is the infectious disease in oral cavity caused by the interaction between host and periodontopathogenic bacteria. Porphyromonas gingivalis (Pg) is related to periodontitis. As previously reported, Pg also shows the strong correlation with systemic disease as diabetic mellitus (DM), arteriosclerosis, obesity, non-alcoholic steatohepatitis (NASH), cardiovascular disturbance and rheumatoid arthritis (RA). The clarification of Pg effect in the onset of systemic disease is necessary to confirm the etiological findings. RA model mice with Pg infection was established and molecular mechanism involved in the onset of RA by Pg infection was determined. In order to establish RA mice, SKG mice (CREA Japan) were received i.p. injection of laminarin. Pg was also infected into mice via i.p. injection. The Pg infected RA mice (Pg-RA mice) showed severe symptoms compared with RA mice without Pg infection in the joint swelling and bone disruption in ankle joint. The infiltration of immune cell and pannus, which was inflammatory dysplasia of RA joint tissue in synovial cell, and a number of osteoclasts were observed. The inflammatory cytokine, anti-cyclic citrullinated peptide antibody in Pg-RA mice were elevated compared with RA mice. Furthermore, the mRNA expression of osteoclastogenesis related gene was induced in the bone marrow cell from Pg-RA mice. These findings show that Pg infection has potential for RA exacerbation.
  7. The analysis of clinical factors of Rheumatoid Arthritis in SKG mice with Porphyromonas gingivalis oral administration, Shuichi Munenaga, Kazuhisa Ouhara, Masahiro Yamakawa, Satoshi Yamazaki, Hidemi Kurihara, The 60th Annual Gereral Assembly and Scientific Meeting of the Japan College of Rheumatology, 2016/04/21, Without Invitation, Japanese, Yokohama-city
  8. The 59th spring meting of the Japanese society of periodontology, Kazuhisa Ouhara, Yuushi Uchida, Noriyoshi Mizuno, Tsuyoshi Fujita, Hidemi Kurihara, The 59th spring meting of the Japanese society of periodontology, 2016/05/20, Without Invitation, Japanese, JSPS, Kagoshima-city
  9. Evaluation of periodontitis with Porphyromonas gulae infection in debetes model mice, Keiichi Akutagawa, Tsuyoshi Fujita, Yuushi Uchida, Tasuku Takemura, Kazuhisa Ouhara, Mikihito Kajiya, Shinji Matsuda, Noriyoshi Mizuno, Hidemi Kurihara, The 59th spring meeting of the Japanese society of Periodontology, 2016/05/21, Without Invitation, Japanese, JSPS, Kagoshima-cuty
  10. The effect of cryopreservation on bone regenerative capacity of Clumps of MSCs/ECM complex, Souta Motoike, Mikihito Kajiya, Kei Takeshita, Manabu Takewaki, Tomoyuki Iwata, Katsuhiro Takeda, Kazuhisa Ouhara, Tsuyoshi Fujita, Hidemi Kurihara, The 59th spring meeting of the Japanese society of Periodontology, 2016/05/21, Without Invitation, Japanese, JSPS, Kagoshima-city
  11. Mechanotransduction in 3D culture clumps of a MSC/ECM complex, Nao Komatsu, Mikihito Kajiya, Kei Takeshita, Manabu Takewaki, Souta Motoike, Kazuhisa Ouhara, Katsuhiro Takeda, Tomoyuki Iwata, Tsuyoshi Fujita, Hidemi Kurihara, The 37th Annual Meeting of the Japanese Society of Inflammation and Regeneration, 2016/06/16, Without Invitation, Japanese, the Japanese Society of Inflammation and Regeneration, Kyoto-city
  12. Porphyromonas gingivalis infection exacerbates rheumatoid arthritis in mice model., Masahiro Yamakawa, Kazuhisa Ouhara, Takata Takashi, Hidemi Kurihara, IADR/APR General Session & Exhibition – Seoul, Republic of Korea, 2016/06/22, With Invitation, English
  13. The reactivity of surface antigen ofporphyromonas gingivalis and serumderived from the patient with rheumatois arthritis, Kazuhisa Ouhara, Shuichi Munenaga, Masahiro Yamakawa, Yuta Hamamoto, Noriyoshi Mizuno, Tsuyoshi Fujita, Hidemi Kurihara, The 8th meting of the Japanese society of periodontology in Cyugoku, 2016/07/03, Without Invitation, Japanese, JSPS
  14. Characterization of Liver Cancer and Periodontal Disease by a Breath Gas Component Analysis, Hiroaki Tokutake, Yuuiti Sakumura, Mutsumi Miyauchi, Kazuhisa Ouhara, Hidemi Kurihara, Takashi Takata, Usoku Shin, Akiko Tanaka, Kazuo Sato, 8th Annual meeting of Japan society for medical application of stable isotopeand biogas, 2016/10/14, Without Invitation, Japanese, Japan Society for Medical Application of Stable Isotope and Biogas, Tokyo
  15. The oriental medicine, magnoliaceae (Kouboku), inhibits the production of methyl mercaptan via mgl mRNA inhibition., Yoko Sato, Kazuhisa Ouhara, Shuichi Munenaga, Mikihito Kajiya, Noriyoshi Mizuno, Tsuyoshi Fujita, Hidemi  Kurihara, the 145th Meeting of The Japanese Society of Conservation Dentistry, 2016/10/27, Without Invitation, Japanese, The Japanese Society of Conservation Dentistry, Matsumoto-city
  16. The stabilization of inflammatory cytokine mRNA by HuR in human gingival epithelial cell, Kazuhisa Ouhara, Syuichi Munenaga, 60th annual meeting of Japanese Society of Periodontology, 2017/05/12, Without Invitation, Japanese, Japanese Society of Periodontology, Fukuoka
  17. The effect of oral inoculation of different type FimA Porphyromonas gingivalis to SKG mouse in the progression of RA, Yuta Hamamoto, Kazuhisa Ouhara, Annual meeting of Japanese Society of Periodontology, 2017/12/15, Without Invitation, Japanese, Japanese Society of Periodontology, Kyoto
  18. The effect of C5a on the progression of arthritis by Porphyromonas gingivalis infection, Syuichi Munenaga, Kazuhisa Ouhara, 60th Annual meeting of Japanese Society of Periodontology, 2017/12/15, Without Invitation, Japanese, Japanese Society of Periodontology, Kyoto
  19. The oriental medicine, magnoliaceae, inhibits the production of methylmercaptan via mgl mRNA., Yoko Sato, Kazuhisa Ouhara, 60th annual meeting of Japanese Society of Periodontology, 2017/12/15, Without Invitation, Japanese
  20. The localization of ACPA derived from RA patient in periodontal tissue of mouse periodontitis model, Kazuhisa Ouhara,Syuichi Munenaga, Tatsuomi Kuranobu, Eiji Sugiyama, Hidemi Kurihara, Syuichi Munenaga, Tatsuomi Kuranobu, Eiji Sugiyama, Hidemi Kurihara, The 62nd Annual General Assembly and Scientific Meeting of the Japan College of Rheumatology, 2018/04/26, Without Invitation, Japanese, experimental
  21. A Human ACPA Monoclonal Antibody Is Preferably Localized at Inflammatory Gingival Tissue and Activates Osteoclastogenensis in Porphyromonas Gingivalis Infected SKG Mouse, Kazuhisa Ouhara, Tatsuhiko Ozawa, Syuichi Munenaga, Tatsuomi Kuranobu, Yuta Hamamoto, Toshihisa Kawai, Eiji Sugiyama, Hidemi Kurihara, 2018 ACR/ARHP Annual Meeting, 2018/10/19, Without Invitation, English, Chicago, USA, experimental
  22. The mechanism of induction of rheumatois arthritis by ACPA, Kazuhisa Ouhara, Osteoimmunology Seminor 2018 Second Half, 2018/12/13, With Invitation, Japanese, experimental
  23. Involvement of C5a in the progression of experimental arthritis in SKG mice induced by Porphyromonas gingivalis infection., Syuichi Munenaga, Kazuhisa Ouhara, Mikihito Kajiya, Katsuhiro Takeda, Noriyoshi Mizuno, Tsuyoshi Fujita, Hidemi Kurihara, 7th Hiroshima Conference, 2018/03/29, Without Invitation, English, experimental
  24. The effect of dysbiosis in gut for aggravation of synovitis in the mouse models of rheumatoid arthritis with Porphyromonas gingivalis infection, Yuta Hamamoto, Kazuhisa Ouhara, Syuichi Munenaga, Mikihito Kajiya, Noriyoshi Mizuno, Tsuyoshi Fujita, Hidemi Kurihara, 61th annual meeting of the japanese society of periodontology, 2018/06/01, Without Invitation, Japanese, experimental
  25. The mechanism of ACPA as a pathogenic factor in the progression of rheumatoid arthritis, Kazuhisa Ouhara, Syuichi Munenaga, Yuta Hamamoto, Shintaro Hirata, Hidemi Kurihara, Eiji Sugiyama, 14th Young Researchers in Advanced Rheumatology (YRAR), 2019/01/05, Without Invitation, Japanese, Osaka
  26. Mechanotransduction in 3D Culture Clumps of Mesenchymal Stem Cells/Extracellular Matrix Complexes Regulates the Cell Fate, 2018/03/29, Without Invitation, English
  27. Morphological change and cellular response of periodontal tissues in ligature-induced experimental periodontitis in mice, The 5th joint scientific meeting in dentistry, 2018/10/02, Without Invitation, English, joint scientific meeting in dentistry
  28. Identification of a causative gene in a family case with aggressive periodontitis by exome sequencing and linkage analysis, 2018/10/20, Without Invitation, English, Korean Academy of Periodontology, seoul
  29. Serum antibody titer against FimA Type IV of Porphyromonas gingivalis is a possible marker for preterm birth cause by periodontitis., Hikaru Takagi, H. Furusho, M. Miyauchi, K. Ouhara, T. Fujita, S. Urabe, T. Shintani, H. Kurihara, Y. Kudo, T. Takata, The 66th Annual Meeting of Japanese Association for Dental Research, 2018/11/17, Without Invitation, English, Japanese Association for Dental Research

Awards

  1. 2018/11/17, The 66th Japanese Association for Dental Research / Joseph Lister Award 2018 First prize, Japanese Association for Dental Research President

External Funds

Acceptance Results of Competitive Funds

  1. Planning research research grant, Epidemiological study on the safety and effectiveness of periodontal disease treatment in patients taking anticoagulants, 2022, 2023
  2. KAKENHI(Grant-in-Aid for Scientific Research (C)), 2022, 2024
  3. KAKENHI, 2013, 2014
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  4. KAKENHI, 2012, 2014
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  5. KAKENHI, The analysis and application for periodontal treatment of FcRn which is tha passive IgG transporter in epithelial cells, 2010, 2012
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  6. KAKENHI, 2009, 2010
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  7. KAKENHI, 2015, 2017
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  8. KAKENHI, 2013, 2015
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  9. KAKENHI, 2015, 2017
  10. KAKENHI(Grant-in-Aid for Scientific Research (C)), 2018, 2020

Social Activities

History as Peer Reviews of Academic Papers

  1. 2014, Journal of Indian Society of Periodontology, Others, 4
  2. 2014, journal of periodontal research, Others, reviewer, 1