RYOTA NAKAZATO

Last Updated :2023/01/05

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Medicine), Assistant Professor
E-mail
nakazatohiroshima-u.ac.jp
Self-introduction
Research topic : Anatomy and Developmental Biology with Clock genes. I try to educate students the understanding four-dimensionally the human body structure through lectures of "human body anatomy for human understanding", "systematic anatomy", and "embryology".

Basic Information

Major Professional Backgrounds

  • 2019/.7, Hiroshima University, Graduate School of Biomedical and Health Sciences, Assistant Professor
  • 2019/.4, 2019/.6, Okayama University, Researcher
  • 2018/.1, 2019/.3, Osaka University, Research Assistant Professor
  • 2016/.5, 2018/.9, Cancer Research Institute of Kanazawa University, Assistant Professor
  • 2015/.4, 2016/.4, Cancer Research Institute of Kanazawa University, Postdoctoral Fellow
  • 2012/.4, 2015/.3, JSPS, Special Researcher

Educational Backgrounds

  • Kanazawa University, Graduate School of Medical Science, Doctor course, School of Pharmaceutical Sciences, Japan, 2012/.4, 2015/.3
  • Kanazawa University, Graduate School of Medical Science, Master course, School of Pharmaceutical Sciences, Japan, 2010/.4, 2012/.3
  • Kanazawa University, Pharmaceutical Department, School of Pharmaceutical Sciences, Japan, 2006/.4, 2010/.3

Academic Degrees

  • Kanazawa University
  • PhD, Kanazawa University

Educational Activity

  • [Bachelor Degree Program] School of Medicine : Program of Medicine : Medicine
  • [Master's Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Biomedical Science
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Biomedical Science
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Biomedical Sciences : Program of Medicine

In Charge of Primary Major Programs

  • Medicine
  • Life Sciences

Research Fields

  • Biology;Biological Science;Cell biology
  • Medicine,dentistry, and pharmacy;Basic medicine;General anatomy (including histology / embryology)

Research Keywords

  • Circadian clock
  • Cilia
  • extracellular vesicles

Affiliated Academic Societies

  • The Japanese Pharmacological Society
  • The Molecular Biology Society of Japan
  • Japanese cancer association

Educational Activity

Course in Charge

  1. 2022, Liberal Arts Education Program1, 4Term, Anatomy for understanding human being II
  2. 2022, Undergraduate Education, First Semester, Structure of human body
  3. 2022, Graduate Education (Master's Program) , First Semester, Seminar
  4. 2022, Graduate Education (Master's Program) , Second Semester, Seminar
  5. 2022, Graduate Education (Master's Program) , First Semester, Research
  6. 2022, Graduate Education (Master's Program) , Second Semester, Research
  7. 2022, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar
  8. 2022, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar
  9. 2022, Graduate Education (Doctoral Program) , First Semester, Advanced Research
  10. 2022, Graduate Education (Doctoral Program) , Second Semester, Advanced Research
  11. 2022, Graduate Education (Master's Program) , First Semester, Human Anatomy
  12. 2022, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Anatomy and Developmental Biology
  13. 2022, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Anatomy and Developmental Biology
  14. 2022, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Anatomy and Developmental Biology
  15. 2022, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Anatomy and Developmental Biology

Research Activities

Academic Papers

  1. A pair of primers facing at the double-strand break site enables to detect NHEJ-mediated indel mutations at a 1-bp resolution, SCIENTIFIC REPORTS, 12(1), 20220708
  2. A negative feedback loop between JNK-associated leucine zipper protein and TGF-B1 regulates kidney fibrosis, Communications Biology, 3(288), 20200605
  3. Inhibition of the glutamine transporter SNAT1 confers neuroprotection in mice by modulating the mTOR-autophagy system, Communications Biology, 2(346), 20190918
  4. Mechanism of release of cilia-derived vesicles and function of the vesicles, 87(5), 692-696, 201911
  5. JLP-JNK signaling protects cancer cells from reactive oxygen species-induced cell death., Biochemical and biophysical research communications, 501(3), 724-730, 2018
  6. Selective upregulation of Per1 mRNA expression by ATP through activation of P2X7 purinergic receptors expressed in microglial cells., Journal of pharmacological sciences, 116(4), 350-361, 2011
  7. Requirement of both NR3A and NR3B subunits for dominant negative properties on Ca2+ mobilization mediated by acquired N-methyl-D-aspartate receptor channels into mitochondria., Neurochemistry international, 57(7), 730-737, 2010
  8. Gradual downregulation of protein expression of the partner GABA(B)R2 subunit during postnatal brain development in mice defective of GABA(B)R1 subunit., Journal of pharmacological sciences, 115(1), 45-55, 2011
  9. Exacerbated vulnerability to oxidative stress in astrocytic C6 glioma cells with stable overexpression of the glutamine transporter slc38a1., Neurochemistry international, 58(4), 504-511, 2011
  10. A possible pivotal role of mitochondrial free calcium in neurotoxicity mediated by N-methyl-d-aspartate receptors in cultured rat hippocampal neurons., Neurochemistry international, 59(1), 10-20, 2011
  11. Selective downregulation of N-methyl-D-aspartate receptor (NMDAR) rather than non-NMDAR subunits in ipsilateral cerebral hemispheres in rats with middle cerebral artery occlusion., Nihon shinkei seishin yakurigaku zasshi = Japanese journal of psychopharmacology, 31(4), 187-194, 2011
  12. Possible involvement of mitochondrial uncoupling protein-2 in cytotoxicity mediated by acquired N-methyl-D-aspartate receptor channels., Neurochemistry international, 61(4), 498-505, 2012
  13. Possible neuroprotective property of nicotinic acetylcholine receptors in association with predominant upregulation of glial cell line-derived neurotrophic factor in astrocytes., Journal of neuroscience research, 90(11), 2074-2085, 2012
  14. Promoted neuronal differentiation after activation of alpha4/beta2 nicotinic acetylcholine receptors in undifferentiated neural progenitors., PloS one, 7(10), e46177, 2012
  15. An analysis of skeletal development in osteoblast-specific and chondrocyte-specific runt-related transcription factor-2 (Runx2) knockout mice., Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 28(10), 2064-2069, 2013
  16. Selective inhibition by ethanol of mitochondrial calcium influx mediated by uncoupling protein-2 in relation to N-methyl-D-aspartate cytotoxicity in cultured neurons., PloS one, 8(7), e69718, 2013
  17. Constitutive and functional expression of runt-related transcription factor-2 by microglial cells., Neurochemistry international, 74, 24-35, 2014
  18. Upregulation of Runt-Related Transcription Factor-2 Through CCAAT Enhancer Binding Protein-b Signaling Pathway in Microglial BV-2 Cells Exposed to ATP., Journal of cellular physiology, 230(10), 2510-2521, 2015
  19. Daily oral intake of theanine prevents the decline of 5-bromo-2'-deoxyuridine incorporation in hippocampal dentate gyrus with concomitant alleviation of behavioral abnormalities in adult mice with severe traumatic stress., Journal of pharmacological sciences, 127(3), 292-297, 2015
  20. Upregulation of Slc38a1 Gene Along with Promotion of Neurosphere Growth and Subsequent Neuronal Specification in Undifferentiated Neural Progenitor Cells Exposed to Theanine., Neurochemical research, 41(1-2), 5-15, 2016
  21. Critical role of JSAP1 and JLP in axonal transport in the cerebellar Purkinje cells of mice., FEBS letters, 589(19 Pt B), 2805-2811, 2015
  22. Genetic analysis of Runx2 function during intramembranous ossification., Development (Cambridge, England), 143(2), 211-218, 2016
  23. ★, The intrinsic microglial clock system regulates interleukin-6 expression., Glia, 65(1), 198-208, 2017
  24. Bone Resorption Is Regulated by Circadian Clock in Osteoblasts., Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 32(4), 872-881, 2017
  25. Scaffold protein JLP mediates TCR-initiated CD4+T cell activation and CD154 expression., Molecular immunology, 87, 258-266, 2017
  26. Critical role of glioma-associated oncogene homolog 1 in maintaining invasive and mesenchymal-like properties of melanoma cells., Cancer science, 108(8), 1602-1611, 2017
  27. ★, Disruption of Bmal1 Impairs Blood-Brain Barrier Integrity via Pericyte Dysfunction., The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(42), 10052-10062, 2017
  28. Possible activation by the green tea amino acid theanine of mammalian target of rapamycin signaling in undifferentiated neural progenitor cells in vitro., Biochemistry and biophysics reports, 5, 89-95, 2016

Invited Lecture, Oral Presentation, Poster Presentation

  1. Functional analysis of JSAP in the regulation of chromosome segregation, Consortium of Biological Sciences 2017, 2017/12/06, Without Invitation, Japanese
  2. Role of JSAP in cell division control and cancer, The 75th Annual Meeting of the Japanese Cancer Association, 2016/10/06, Without Invitation, Japanese, Proper cell cycle regulation is essential for normal cell function, and its dysregulation is often associated with cellular transformation and tumorigenesis. We previously showed that the JSAP group of proteins, JSAP1 and JSAP2, plays a role in cytokinesis, the final stage of cell division. In addition, recent studies have reported that overexpression of JSAP2 is correlated with poor prognosis in hepatocellular carcinoma and non-small cell lung cancer. To date, however, functional role of JSAP in cell division control and cancer remains largely unknown. In this study, we investigated the effect of high expression of JSAP on cultured cells. Overexpression of wild-type JSAP1 or JSAP2 (JSAP1_WT or JSAP2_WT), but not mutant JSAP1 or JSAP2 lacking kinesin-1 heavy chain-binding domain (KBD) (JSAP1_ΔKBD or JSAP2_ΔKBD), caused nuclear morphological abnormalities. Furthermore, prolonged M phase and cell death were observed in HeLa cells overexpressing JSAP_WT, but not JSAP_ΔKBD. Together, these findings may suggest that JSAP plays a critical role in the regulation of mitosis, particularly chromosome segregation through interaction with kinesin-1.
  3. Critical role of the scaffold protein JSAP in the regulation of cell division, 2015/12/01, Without Invitation, Japanese
  4. A role of the osteoblastogenesis master regulator, runt-related transcription factor-2, in microglial cells, Ryota Nakazato, Takeshi Takarada and Yukio Yoneda, 2014/09/29, Without Invitation, Japanese
  5. Proliferation and differentiation of neural progenitors exposed to nicotine, Ryota Nakazato, Takeshi Takarada and Yukio Yoneda, ISAM 2014, 2014/10/03, Without Invitation, English, Yokohama, Japan
  6. A role of the osteoblastic master regulator, runt-related transcription factor-2, in microglial cells, Ryota Nakazato, Takeshi Takarada and Yukio Yoneda, 2014/03/19, Without Invitation, Japanese
  7. Upregulation of Runx2 expression through activation of P2X7 receptors in microglial cells, Ryota Nakazato, Takeshi Takarada and Yukio Yoneda, 2013/06/20, Without Invitation, Japanese
  8. Upregulation by ATP of Runx2 expression in microglial cells, Nakazato R., Takarada T., Yoneda Y., 24th Meeting of the International Society for Neurochemistry, 2013/04/20, Without Invitation, English, Cancun (Mexico)
  9. Expression of runt related transcription factor-2 in microglial cells, Ryota Nakazato, Takeshi Takarada and Yukio Yoneda, 11th Biennial Meeting of APSN and 55th Meeting of JSN, 2012/10/02, Without Invitation, English, Kobe, Japan

Awards

  1. 2012/10/02, Best Student Poster Presentation Award 2012 11 th Biennial Meeting of APSN and 55th Meeting of JSN, APSN

External Funds

Acceptance Results of Competitive Funds

  1. Grant-in-Aid for Early-Career Scientists, The role of clock genes in cell communication, 2020, 2022
  2. Grant-in-Aid for Young Scientists (B), 2017, 2018
  3. Grant-in-Aid for Research Activity start-up, 2015, 2017
  4. Grant-in-Aid for JSPS Fellows, 2012, 2015