KANA HARADA

Last Updated :2025/02/03

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Medicine), Assistant Professor
Web Site
E-mail
haradakhiroshima-u.ac.jp
Other Contact Details
1-2-3 Kasumi, Minami-ku, Hiroshima, Japan, Japan
TEL : (+81)82-257-5142 FAX : (+81)
Self-introduction
My research interests are the mechanisms regulating the function of serotonin transporter, a therapeutic target of depression.

Basic Information

Major Professional Backgrounds

  • 2011/04/01, 2013/02/28, Hiroshima University, Graduate School of Biomedical & Health Sciences, Assistant Professor (Special Appointment)
  • 2013/03/01, 2014/03/31, Hiroshima University, Graduate School of Biomedical & Health Sciences, Researcher
  • 2014/04/01, 2018/03/31, Hiroshima International University, Assistant Professor (Special Appointment)
  • 2018/04/01, 2019/03/31, Hiroshima University, Graduate School of Biomedical & Health Sciences, Assistant Professor
  • 2019/04/01, Hiroshima University, Graduate School of Biomedical & Health Sciences, Assistant Professor

Educational Backgrounds

  • Hiroshima University, Graduate School of Biomedical Sciences, Japan, 2007/10, 2011/03
  • Hiroshima University, Graduate School of Biomedical Sciences, Japan, 2007/04, 2007/09
  • Hiroshima University, Graduate School of Biomedical Sciences, Japan, 2005/04, 2007/03
  • Hiroshima University, Faculty of Medicine, Japan, 2001/04, 2005/03

Academic Degrees

  • Hiroshima University
  • Ph.D., Hiroshima University

Educational Activity

  • [Bachelor Degree Program] School of Medicine : Program of Medicine : Medicine
  • [Master's Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Biomedical Science
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Biomedical Science
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Biomedical Sciences : Program of Medicine

Research Fields

  • Medicine,dentistry, and pharmacy;Basic medicine;General pharmacology
  • Medicine,dentistry, and pharmacy;Dentistry;Prosthodontics / Dental materials science and

Research Keywords

  • serotonin transporter
  • polyphosphate
  • macrophage
  • microglia
  • bone

Affiliated Academic Societies

  • The Japanese Pharmacological Society
  • The Japanese Society for Neurochemistry
  • The Pharmaceutical Society of Japan

Educational Activity

Course in Charge

  1. 2024, Undergraduate Education, Year, Biological Responses
  2. 2024, Undergraduate Education, Year, Methodology in advanced medical sciences

Research Activities

Academic Papers

  1. Protein kinase C (PKC) inhibitor Calphostin C activates PKC in a light-dependent manner at high concentrations via the production of singlet oxygen, EUROPEAN JOURNAL OF PHARMACOLOGY, 984, 20241205
  2. ★, S-Palmitoylation of the serotonin transporter promotes its cell surface expression and serotonin uptake, Biochemical and Biophysical Research Communications, 662, 58-65, 20230625
  3. Features and mechanisms of propofol-induced protein kinase C (PKC) translocation and activation in living cells, FRONTIERS IN PHARMACOLOGY, 14, 20231107
  4. Identification of protein kinase C domains involved in its translocation induced by propofol, EUROPEAN JOURNAL OF PHARMACOLOGY, 955, 20230915
  5. P2Y2 receptor mediates dying cell removal via inflammatory activated microglia, JOURNAL OF PHARMACOLOGICAL SCIENCES, 153(1), 55-67, 202309
  6. GPR3 expression in retinal ganglion cells contributes to neuron survival and accelerates axonal regeneration after optic nerve crush in mice, NEUROBIOLOGY OF DISEASE, 172, 105811, 20221001
  7. Potential role of inducible GPR3 expression under stimulated T cell conditions, JOURNAL OF PHARMACOLOGICAL SCIENCES, 148(3), 307-314, 202203
  8. Effects of flurbiprofen on the functional regulation of serotonin transporter and its misfolded mutant, JOURNAL OF PHARMACOLOGICAL SCIENCES, 148(1), 187-195, 202201
  9. GPR3 accelerates neurite outgrowth and neuronal polarity formation via PI3 kinase-mediating signaling pathway in cultured primary neurons, MOLECULAR AND CELLULAR NEUROSCIENCE, 118, 103691, 202201
  10. Syntaxin 3 interacts with serotonin transporter and regulates its function, JOURNAL OF PHARMACOLOGICAL SCIENCES, 145(4), 297-307, 202104
  11. Role of the E3 ubiquitin ligase HRD1 in the regulation of serotonin transporter function, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 534, 583-589, 20210101
  12. Detailed neuronal distribution of GPR3 and its co-expression with EF-hand calcium-binding proteins in the mouse central nervous system, BRAIN RESEARCH, 1750, 20210101
  13. Component of nicotine-induced intracellular calcium elevation mediated through alpha 3-and alpha 5-containing nicotinic acetylcholine receptors are regulated by cyclic AMP in SH-SY 5Y cells, PLOS ONE, 15(11), 20201130
  14. Propofol induces the elevation of intracellular calcium via morphological changes in intracellular organelles, including the endoplasmic reticulum and mitochondria, EUROPEAN JOURNAL OF PHARMACOLOGY, 884, 20201005
  15. Anti-seizure effect and neuronal activity change in the genetic-epileptic model rat with acute and chronic vagus nerve stimulation, Epilepsy Research, 155, 106159, 201909
  16. Data on the effect of maternal separation coupled with social isolation in a forced swim test and gene expression of glial fibrillary acid protein in the prefrontal cortex of rats, Data in Brief, 18, 496-500, 20180616
  17. Sodium butyrate abolishes lipopolysaccharide-induced depression-like behaviors and hippocampal microglial activation in mice, Brain Research, 1680, 13-38, 20180201
  18. The Toll-like receptor 4-activated neuroprotective microglia subpopulation survives via granulocyte macrophage colony-stimulating factor and JAK2/STAT5 signaling, Neurochemistry International, 93, 82-94, 201602
  19. ★, Phospholipase C-related catalytically inactive protein participates in the autophagic elimination of Staphylococcus aureus infecting mouse embryonic fibroblasts, PLOS ONE, 9, e98285, 20140527
  20. Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion, Biology Open, 3(6), 463-474, 20140508
  21. ★, Polyphosphate-mediated inhibition of tartrate-resistant acid phosphatase and suppression of bone resorption of osteoclasts, PLOS ONE, 8, e78612, 20131104
  22. ★, Inorganic polyphosphate suppresses lipopolysaccharide- induced inducible nitric oxide synthase (iNOS) expression in macrophages, PLOS ONE, 8, e74650, 20130909
  23. ★, Extracellular ATP differentially modulates Toll-like receptor 4-mediated cell survival and death of microglia, Journal of Neurochemistry, 116(6), 1138-1147, 201103
  24. Microglial alpha7 nicotinic acetylcholine receptors drive a phospholipase C/IP3 pathway and modulate the cell activation toward a neuroprotective role, Journal of Neuroscience Research, 83(8), 1461-1470, 200606

Invited Lecture, Oral Presentation, Poster Presentation

  1. Intracellular calcium dynamics induced by Propofol and Nicotine - Observations using the intracellular organelle calcium indicator CEPIA (calcium-measuring organelle-entrapped protein indicators), 2023/12/15, Without Invitation, English
  2. Prolonged exposure to coffee alters serotonin transporter expression in intestinal epithelial cells via DNA methylation, 2023/12/14, Without Invitation
  3. Regulatory mechanism of immediate early G protein-coupled receptor 3 gene expression during neuronal differentiation in PC12 cells, 2023/12/14, Without Invitation
  4. Involvement of G protein-coupled receptor 3 in retinal ganglion neuron survival and axonal regeneration following optic nerve crush in mice, Shun Masuda, Hiroko Shiraki, Yusuke Sotomaru, Kana Harada, Izumi Hide, Yoshiaki Kiuchi, Norio Sakai, Shigeru Tanaka, Neuroscience 2023, 2023/11/15, Without Invitation, English, Washington, D.C.
  5. Detailed analysis of the mechanism underlying propofol-induced PKC translocation and activation., Soma Noguchi, Taketoshi Kajimoto, Takuya Kumamoto, Tomoaki Urabe, Soshi Narasaki, Kana Harada, Izumi Hide, Shigeru Tanaka, Norio Sakai, Neuroscience 2023, 2023/11/14, Without Invitation, English, Washington, D.C.
  6. Uncovering the Influence of Long-Term Coffee Exposure on Intestinal Epithelial Cells' Serotonin Uptake, Satoshi Kikkawa, Serika Imamura, Kana Harada, Shigeru Tanaka, Izumi Hide, Norio Sakai, Neuroscience 2023, 2023/11/13, Without Invitation, English, Washington, D.C.
  7. OpTER: a low-cost method for measuring the transepithelial/endothelial electrical resistance TER of cell layers, which reduces research costs and the burden on us all, Satoshi Kikkawa, Kana Harada, Shigeru Tanaka, Izumi Hide, Norio Sakai, JPW2022 (Japan Basic and Clinical Pharmacology Week 2022), The 96th Annual Meeting of the Japanese Pharmacological Society, The 43rd Annual Scientific Meeting of the Japanese Society of Clinical Pharmacology and Therapeutics, 2022/11/30, Without Invitation, English, Yokohama
  8. OpTER: a Low-Cost, Reproducible and Customizable Method to Assess the Cellular Barrier Function, Satoshi Kikkawa, Kana Harada, Shigeru Tanaka, Izumi Hide, Norio Sakai, NEURO2022, The 45th Annual Meeting of the Japan Neuroscience Society, The 65th Annual Meeting of the Japanese Society for Neurochemistry, The 32nd Annual Conference of the Japanese Neural Network Society, 2022/07/02, Without Invitation, English, Ginowan/Online
  9. Divergent functions of G protein-coupled receptor 3 in neurite outgrowth, polarity formation, survival, and axonal regeneration in neurons, Shigeru Tanaka, Shun Masuda, Naoto Shimada, Hiroko Shiraki, Tatsuhiro Miyagi, Kana Harada, Izumi Hide, Norio Sakai, NEURO2022, The 45th Annual Meeting of the Japan Neuroscience Society, The 65th Annual Meeting of the Japanese Society for Neurochemistry, The 32nd Annual Conference of the Japanese Neural Network Society, 2022/07/01, Without Invitation, English, Ginowan/Online
  10. Spatiotemporal analysis of propofol-induced PKC translocation -Subcellular Localization of PKC Activation by propofol-, Soma Noguchi, Taketoshi Kajimoto, Tomoaki Urabe, Yuki Yanase, Soshi Narasaki, Kana Harada, Shigeru Tanaka, Izumi Hide, Norio Sakai, The 95th Annual Meeting of the Japanese Pharmacological Society, 2022/03/09, Without Invitation, English, Fukuoka
  11. PKC domains involved in propofol-induced PKC translocation, Soshi Narasaki, Soma Noguchi, Tomoaki Urabe, Kana Harada, Izumi Hide, Sigeru Tanaka, Yuki Yanase, Taketoshi Kajimoto, Norio Sakai, The 95th Annual Meeting of the Japanese Pharmacological Society, 2022/03/09, Without Invitation, English, Fukuoka
  12. Diversity and functional roles of G protein-coupled receptor 3 in nonneuronal cells, Shigeru Tanaka, Hiroko Shiraki, Yun Guo, Yuhki Yanase, Kana Harada, Izumi Hide, Tomoharu Yasuda, Norio Sakai, The 95th Annual Meeting of the Japanese Pharmacological Society, 2022/03/09, Without Invitation, English, Fukuoka
  13. Potential role of G protein-coupled receptor 3 in the axonal regeneration after optic nerve injury in mice, Shun Masuda, Shigeru Tanaka, Hiroko Shiraki, Yuusuke Sotomaru, Kana Harada, Izumi Hide, Yoshiaki Kiuchi, Norio Sakai, The 95th Annual Meeting of the Japanese Pharmacological Society, 2022/03/07, Without Invitation, English, Fukuoka
  14. The mechanism underlying the propofol-induced elevation of intracellular calcium, Tomoaki Urabe, Norio Sakai, Serika Motoike, Kana Harada, Izumi Hide, Shigeru Tanaka, Neuroscience 2019, 2019/10/23, Without Invitation, English, Chicago
  15. NECAB2 is a major calcium-binding protein of GPR3-positive neurons in various regions of the mouse brain, Fumiaki Ikawa, Shigeru Tanaka, Kana Harada, Izumi Hide, Norio Sakai, Neuroscience 2019, 2019/10/23, Without Invitation, English, Chicago
  16. GPR3 is upregulated in rodent mast cells immediately after brain ischemia and modulates degranulation, Shigeru Tanaka, Yuhki Hamakawa, Yuhki Yanase, M Yamamoto, Hiroko Shiraki, Kana Harada, Izumi Hide, Norio Sakai, Neuroscience 2019, 2019/10/20, Without Invitation, English, Chicago
  17. Differentiation-induced G protein-coupled receptor 3 modulates phosphorylation of synapsin in PC12 cells, Humiaki Ikawa, Shigeru Tanaka, Kenta Sasaki, Tomohiro Noguchi, Kana Harada, Izumi Hide, Norio Sakai, NEURO2019 The 42nd Annual Meeting of the Japan Neuroscience Society The 62nd Annual Meeting of the Japanese Society for Neurochemistry, 2019/07/27, Without Invitation, English, Niigata
  18. Expression and function of P2Y2 and P2Y13 receptors in purinergic and Toll-like receptor 4-activated microglia, Izumi Hide, Yoichiro Morita, Hiroko Shiraki, Yuhki Yanase, Kana Harada, Shigeru Tanaka, Norio Sakai, NEURO2019 The 42nd Annual Meeting of the Japan Neuroscience Society The 62nd Annual Meeting of the Japanese Society for Neurochemistry, 2019/07/27, Without Invitation, English, Niigata
  19. The potential role of G protein-coupled receptor 3 in mast cell degranulation following brain ischemia, Shigeru Tanaka, Yuhki Yanase, Yuhki Hamakawa, Masahiro Yamamoto, Hiroko Shiraki, Kana Harada, Izumi Hide, Norio Sakai, NEURO2019 The 42nd Annual Meeting of the Japan Neuroscience Society The 62nd Annual Meeting of the Japanese Society for Neurochemistry, 2019/07/26, Without Invitation, English, Niigata
  20. Intrinsic expresson of G protein-coupled receptor 3 accelerates the axon specification in cultured hippocampal neurons, Tanaka Shigeru, Naoto Shimada, Hiroko Shiraki, Tatsuhiro Miyagi, Kana Harada, Izumi Hide, Norio Sakai, The 92nd Annual Meeting of the Japanese Pharmacological Society, 2019/03/15, Without Invitation, Japanese, Osaka
  21. The mechanism underlying the propofol-induced elevation of intracellular calcium, Tomoaki Urabe, Kana Harada, Izumi Hide, Shigeru Tanaka, Masashi Kawamoto, Norio Sakai, The 92nd Annual Meeting of the Japanese Pharmacological Society, 2019/03/14, Without Invitation, English, Osaka
  22. c-Fos immunoreactivity in the rat brain induced by vagus nerve stimulation, Masaya Katagiri, Koji Iida, Kumatoshi Ishihara, Kana Harada, Kota Kagawa, Akira Hashizume, Ryosuke Hanaya, Kazunori Arita, Kaoru Kurisu, 11th Asian & Oceanian Epilepsy Congress, 2016/05/15, Without Invitation, English, HongKong
  23. Induction of c-Fos immunoreactivity by vagus nerve stimulation in the rat brain, Masaya Katagiri, Koji Iida, Kumatoshi Ishihara, Kana Harada, Kota Kagawa, Akira Hashizume, Ryosuke Hanaya, Kazunori Arita, Kaoru Kurisu, American Epilepsy Society 69th Annual meeting, 2015/12/04, Without Invitation, English, Philadelphia
  24. Induction of c-Fos immunoreactivity by vagus nerve stimulation in the rat brain, Masaya Katagiri, Koji Iida, Kumatoshi Ishihara, Kana Harada, Kota Kagawa, Akira Hashizume, Ryosuke Hanaya, Kazunori Arita, Kaoru Kurisu, The 49th Congress of the Japan Epilepsy Society, 2015/10/30, Without Invitation, English, Nagasaki
  25. PRIP modulates autophagosomal maturation containing invasive bacteria, Kae Harada, Kana Harada, Seiya Hayashi, Hanae Ikeda, Takashi Kanematsu, 5th Hiroshima Conference on Education and Science in Dentistry, 2013/10/12, Without Invitation, English, Hiroshima
  26. Enhanced survival and phagocytic activity by Toll-like receptor 4 activation in rat microglia, Izumi Hide, Yuhki Yanase, Mayumi Kamigaki, Kana Harada, Takahiro Seki, Shigeru Tanaka, Michihiro Hide, Norio Sakai, The 11th Biennial Meeting of the Asian-Pacific Society for Neurochemistry/55th Annual Meeting of the Japanese Society for Neurochemistry, 2012/10/01, Without Invitation, Kobe
  27. Toll-like receptor 4 activation promotes survival and phagocytic clearance in microglia: Possible involvement of purinergic receptors, Izumi Hide, Kana Harada, Yuhki Yanase, Takahiro Seki, Shigeru Tanaka, Norio Sakai, Purine 2012, 2012/06/01, Without Invitation, English, Fukuoka
  28. Death or survival of Toll-like receptor 4-activated microglia is regulated by extracellular ATP, Kana Harada, Izumi Hide, Shunsuke Fujiwara, Takahiro Seki, Shigeru Tanaka, Yoshihiro Nakata, Norio Sakai, Neuro 2010 (Joint conference of the 33rd Annual Meeting of the Japan Neuroscience Society, the 53rd Annual Meeting of the Japanese Society for Neurochemistry, and the 20th Annual Meeting of Japanese Neural Network Society), 2010/09/03, Without Invitation, English, Kobe
  29. alpha7 nicotinic acetylcholine receptor signaling and modulation of cytokine production in microglia, Izumi Hide, Kana Harada, Hiroaki Matsubayashi, Norio Sakai, Yoshihiro Nakata, USA-JAPAN joint meeting for Glial Research, 2008/03/19, Without Invitation, English, Philadelphia
  30. Immunohistochemical assessment of neuronal activity in rat thalamus with vagus nerve stimulation, 2014/10, Without Invitation

Awards

  1. 2014/08/09, Young Investigators Award, 日本病態プロテアーゼ学会, オートファジー系を介した黄色ブドウ球菌排除機構におけるPRIPの役割解明

External Funds

Acceptance Results of Competitive Funds

  1. Grants-in-Aid for Scientific Research, 2022, 2024
  2. Grants-in-Aid for Scientific Research, 2019, 2021
  3. Grants-in-Aid for Scienitific Research, 2019, 2021
  4. Grants-in-Aid for Scientific Research, 2016, 2018
  5. Grants-in-Aid for Scientific Research, Effects of polyphosphate on inflammatory bone resorption: Development of novel treatment strategies for peri-implantitis, 2014, 2015
  6. Grants-in-Aid for Scientific Research, A study for the application of inorganic polyphosphate in peri-implantitis treatment, 2011, 2012

Social Activities

History as Peer Reviews of Academic Papers

  1. 2023, Journal of Pharmacological Sciences, Others, Reviewer, 1