Kenji Arakawa

Last Updated :2022/08/02

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Associate Professor
Web Site
E-mail
karakawahiroshima-u.ac.jp
Other Contact Details
1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan
TEL : (+81)81-(0)82-42 FAX : (+81)81-(0)82-42

Basic Information

Major Professional Backgrounds

  • 1999/01/01, 1999/09/30, JSPS, Research Fellow (DC)
  • 1999/10/01, 1999/10/31, JSPS, Research Fellow (PD)
  • 1999/11/01, 2002/03/31, University of Washington, Department of Chemistry, Faculty of Science, Research Associate
  • 2002/04/01, 2007/03/31, Hiroshima University, Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Research Assistant Professor
  • 2007/04/01, 2010/08/31, Hiroshima University, Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Research Assistant Professor
  • 2010/09/01, 2019/03/31, Hiroshima University, Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Associate Professor

Educational Backgrounds

  • Tokyo Institute of Technology, Graduate School, Division of Science and Engineering, Department of Chemistry, Japan, 1997/04, 1999/09
  • Tokyo Institute of Technology, Graduate School, Division of Science and Engineering, Department of Chemistry, Japan, 1995/04, 1997/03
  • Tokyo Institute of Technology, Faculty of Science, Department of Chemistry, Japan, 1991/04, 1995/03

Academic Degrees

  • Doctor of Science, Tokyo Institute of Technology
  • Master of Science, Tokyo Institute of Technology

Research Fields

  • Agricultural sciences;Agricultural chemistry;Bioorganic chemistry

Research Keywords

  • biosynthesis

Educational Activity

Course in Charge

  1. 2022, Liberal Arts Education Program1, 2Term, Introduction to Applied Chemistry, Chemical Engineering,and Biotechnology
  2. 2022, Undergraduate Education, First Semester, Training of Biotechnology I
  3. 2022, Undergraduate Education, 1Term, Bioorganic Chemistry
  4. 2022, Graduate Education (Master's Program) , 1Term, Integrated Genome Science A
  5. 2022, Graduate Education (Master's Program) , Academic Year, Seminar in Biotechnology
  6. 2022, Graduate Education (Master's Program) , 1Term, Exercises in Biotechnology A
  7. 2022, Graduate Education (Master's Program) , 2Term, Exercises in Biotechnology A
  8. 2022, Graduate Education (Master's Program) , 3Term, Exercises in Biotechnology B
  9. 2022, Graduate Education (Master's Program) , 4Term, Exercises in Biotechnology B
  10. 2022, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biotechnology
  11. 2022, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences

Research Activities

Academic Papers

  1. ★, Isolation, biosynthetic investigation, and biological evaluation of maniwamycin G, an azoxyalkene compound from Streptomyces sp. TOHO-M025, Journal of Natural Products, 20220611
  2. Three 4-monosubstituted butyrolactones from a regulatory gene mutant of Streptomyces rochei 7434AN4, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 133(4), 329-334, 202204
  3. Discovery of a poly-amino acid antibiotic solely comprising of L-beta-lysine by potential producer prioritization-guided genome mining, ACS Chemical Biology, 202112
  4. Chemoenzymatic synthesis, computational investigation, and antitumor activity of monocyclic lankacidin derivatives, Bioorganic & Medicinal Chemistry, 202112
  5. Overexpression of SRO_3163, a homolog of Streptomyces antibiotic regulatory protein, induces the production of novel cyclohexene-containing enamide in Streptomyces rochei, Biosci. Biotechnol. Biochem., 202112
  6. Production of agrocinopine A by Ipomoea batatas agrocinopine synthase in transgenic tobacco and its effect on the rhizosphere microbial community, Molecular Plant-Microbe Interactions, 202109
  7. Substrate specificity of two cytochrome P450 monooxygenases involved in lankamycin biosynthesis, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 85(1), 115-125, 20210107
  8. Functional Analysis of P450 Monooxygenase SrrO in the Biosynthesis of Butenolide-Type Signaling Molecules in Streptomyces rochei, BIOMOLECULES, 10(9), 202009
  9. SrrB, a Pseudo-Receptor Protein, Acts as a Negative Regulator for Lankacidin and Lankamycin Production inStreptomyces rochei, FRONTIERS IN MICROBIOLOGY, 11, 1089, 20200609
  10. Relationship between dispersion-forming capability of poly(4-vinylaniline) colloids and antimicrobial activity, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 596, 20200705
  11. ★, The genome sequence of Streptomyces rochei 7434AN4, which carries a linear chromosome and three characteristic linear plasmids, Scientific Reports, 9, 10973-1-10973-11, 20190729
  12. Influence of the size of polystyrene synthesized through soap-free emulsion polymerization on antimicrobial activity, MATERIALS TODAY COMMUNICATIONS, 20, 201909
  13. Computational Prediction of the Mode of Binding of Antitumor Lankacidin C to Tubulin, ACS OMEGA, 4(2), 4461-4471, 201902
  14. Preparation of PVA/polymer colloid nanocomposite hydrogel using PS-PNVA particles, Chemistry Letters, 48(4), 378-381, 201901
  15. Antimicrobial activities of low molecular weight polymers synthesized through soap-free emulsion polymerization, European Polymer Journal, 109(1), 532-536, 20181201
  16. Antimicrobial activities of polymers synthesized through soap-free emulsion polymerization using a cationic initiator and styrene derivative monomers, Chemistry Letters, 47(11), 1402-1404, 20181101
  17. Quinoprotein dehydrogenase functions at the final oxidation step of lankacidin biosynthesis in Streptomyces rochei 7434AN4, Journal of Bioscience and Bioengineering, 126(2), 145-152, 201808
  18. Manipulation of metabolic pathways controlled by signaling molecules, inducers of antibiotic production, for genome mining in Streptomyces spp., ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 111(5), 743-751, 201805
  19. A flavanone derivative from the Asian medicinal herb (Perilla frutescens) potently suppresses IgE-mediated immediate hypersensitivity reactions, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 483(1), 674-679, 20170129
  20. Indomethacin Induction of Metamorphosis from the Asexual Stage to Sexual Stage in the Moon Jellyfish, Aurelia aurita, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 76(7), 1397-1400, 201207
  21. Isolation of Borrelidin as a Phytotoxic Compound from a Potato Pathogenic Streptomyces Strain, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 76(2), 353-357, 201202
  22. Crystal structure of the synergistic antibiotic pair, lankamycin and lankacidin, in complex with the large ribosomal subunit, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108(7), 2717-2722, 20110215
  23. Isolation, structural elucidation, and biosynthesis of 15-norlankamycin derivatives produced by a type-II thioesterase disruptant of Streptomyces rochei, TETRAHEDRON, 67(29), 5199-5205, 20110722
  24. Regulation of Lankamycin Biosynthesis in Streptomyces rochei by Two SARP Genes, srrY and srrZ, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 74(4), 819-827, 201004
  25. ★, The Butenolide Signaling Molecules SRB1 and SRB2 Induce Lankacidin and Lankamycin Production in Streptomyces rochei, CHEMBIOCHEM, 13(10), 1447-1457, 20120709
  26. Characterization of the Early Stage Aminoshikimate Pathway in the Formation of 3-Amino-5-hydroxybenzoic Acid: The RifN Protein Specifically Converts Kanosamine into Kanosamine 6-phosphate, Journal of the American Chemical Society, 124(36), 10644-10645, 20020401
  27. An Olefin Metathesis Approach to 36- and 72-Membered Archaeal Macrocyclic Membrane Lipids, Journal of the Organic Chemistry, 63(14), 4741-4745, 19980401
  28. 36-Membered Macrocyclic Diether Lipid is Advantageous for Archaea to Thrive under the Extreme Thermal Environments, Bulletin of the Chemical Society of Japan, 74(2), 347-356, 20010401
  29. Biosynthetic studies on the alpha-glucosidase inhibitor acarbose: the chemical synthesis of isotopically labeled 2-epi-5-epi-valiolone analogs, Carbohydrate Research, 338(20), 2075-2082, 20030401
  30. Biosynthesis of the Cyclitol Moiety of Pyralomicin 1a in Nonomuraea spiralis MI178-34F18, Journal of Antibiotics, 55(6), 578-584, 20020401
  31. Highly Thermostable Liposome from 72-Membered Macrocyclic Tetraether Lipid: Importance of 72-Membered Lipid for Archaea to Thrive under Hyperthermal Environments, Chemistry Letters, 440-441, 20010401
  32. Giant Vesicles from 72-Membered Macrocyclic Archaeal Phosphokipid Analogues: Initiation of Vesicle Formation by Molecular Recognition between Membrane Components, Chemistry= European Journal, 6(18), 3351-3358, 20000401
  33. Membrane Properties of Archaeal Macrocyclic Diether Phospholipids, Chemistry= European Journal, 6(4), 645-654, 20000401
  34. Significance of the 72-Membered Macrocyclic Structure Found in Archaeal Membrane Lipids: Model Studies of the Macrocyclic Tetraether Diphospholipids by Calorimetric, 31P NMR, and Electron Microscopic Analyses, Bulletin of the Chemical Society of Japan, 72(7), 1575-1581, 19990401
  35. Specific Deuterium Labeling of Archaeal 36-Membered Macrocyclic Diether Lipid, Bulletin of the Chemical Society of Japan, 71(10), 2419-2426, 19980401
  36. Tightly Packed Membranes Composed of 36-Membered Macrocyclic Diether Phospholipid Found in Archaea Growing under Deep-sea Hydrothermal Vents, Chemistry Letters, 901-902, 19980401
  37. Synthesis of Macrocyclic Phosphates as Models of Archaeal Membrane Lipids. Monolayer and Bilayer Studies, Israel Journal of Chemistry, 22(1), 63-69, 19980401
  38. Synthetic Studies of Archaeal Macrocyclic Tetraether Lipids: Practical Synthesis of 72-Membered Tetraether Model Compounds, Bulletin of the Chemical Society of Japan, 70(10), 2545-2554, 19970401
  39. Total Synthesis of Archaeal 36-Membered Macrocyclic Diether Lipid, Journal of the Organic Chemistry, 62(7), 1924-1933, 19970401
  40. Membrane properties of archaeal phospholipids. effect of macrocyclization, Perspectives in Supramolecular Chemistry, 6, 385-390, 20000401
  41. ★, Cyclization Mechanism for the Synthesis of Macrocyclic Antibiotic Lankacidin in Streptomyces rochei, Chemistry and Biology, 12(2), 249-256, 20050201
  42. Gamma-Butyrolactone autoregulator-receptor system involved in lankacidin and lankamycin production andmorphological differentiation in Streptomyces rochei, Microbiology, 153(6), 1817-1827, 20070601
  43. Characterization of the biosynthetic gene clusters located on the Streptomyces linear plasmid, Actinomycetologica, 24(1), 24-30, 20100601
  44. pSLA2-M of Streptomyces rochei is a composite linear plasmid characterized by self-defense genes and homology with pSLA2-L, Biosci. Biotechnol. Biochem, 75(6), 1147-1153, 20110601
  45. Diversity between PKS and FAS, Nature Chem. Biol., 8(7), 604-605, 20120701
  46. Analysis of the loading and hydroxylation steps in lankamycin biosynthesis in Streptomyces rochei, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 50(6), 1946-1952, 200606
  47. Analysis of modular-iterative mixed biosynthesis of lankacidin by heterologous expression and gene fusion, JOURNAL OF ANTIBIOTICS, 60(11), 700-708, 200711
  48. gamma-butyrolactone-dependent expression of the Streptomyces antibiotic regulatory protein gene srrY plays a central role in the regulatory cascade leading to lankacidin and lankamycin production in Streptomyces rochei, JOURNAL OF BACTERIOLOGY, 190(4), 1308-1316, 200802
  49. Extensive Mutational Analysis of Modular-Iterative Mixed Polyketide Biosynthesis of Lankacidin in Streptomyces rochei, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 73(12), 2712-2719, 200912
  50. The structure of ribosome-lankacidin complex reveals ribosomal sites for synergistic antibiotics, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107(5), 1983-1988, 20100202
  51. The biosynthesis of 3-amino-5-hydroxybenzoic acid (AHBA), the precursor of mC(7)N units in ansamycin and mitomycin antibiotics: a review, JOURNAL OF ANTIBIOTICS, 64(1), 35-44, 201101
  52. The tap-tpg gene pair on the linear plasmid functions to maintain a linear topology of the chromosome in Streptomyces rochei., Molecular microbiology, 95(5), 846-858, 2015
  53. Genetic and biochemical analysis of the antibiotic biosynthetic gene clusters on the Streptomyces linear plasmid., Bioscience, biotechnology, and biochemistry, 78(2), 183-189, 2014
  54. Blockage of the early step of lankacidin biosynthesis caused a large production of pentamycin, citreodiol and epi-citreodiol in Streptomyces rochei, JOURNAL OF ANTIBIOTICS, 68(5), 328-333, 201505
  55. Chromosomal circularization of the model Streptomyces species, Streptomyces coelicolor A3(2), FEMS MICROBIOLOGY LETTERS, 347(2), 149-155, 201310
  56. ★, Isolation and Biosynthesis of an Azoxyalkene Compound Produced by a Multiple Gene Disruptant of Streptomyces rochei, CHEMBIOCHEM, 16(15), 2237-2243, 20151012
  57. Antitumor Activity of Lankacidin Group Antibiotics Is Due to Microtubule Stabilization via a Paclitaxel-like Mechanism, JOURNAL OF MEDICINAL CHEMISTRY, 59(20), 9532-9540, 20161027
  58. Gene disruption analysis of two glycosylation steps in lankamycin biosynthesis in Streptomyces rochei, Actinomycetol., 22(2), 35-41, 2008
  59. Influence of the morphology and size of polystyrene synthesized through soap-free emulsion polymerization on antimicrobial activity, Materials Today Communications, 2019

Publications such as books

  1. 2022/04/01, Natural Products from Actinomycetes – Diversity, Ecology and Drug Discovery –, Regulation of secondary metabolites through signaling molecules in Streptomyces, Springer Nature, 2022, 2022, Scholarly Book, Joint work, Kenji Arakawa, Toshihiro Suzuki, 10.1007/978-981-16-6132-7, 167-183
  2. 1999, Giant Vesicles, Membrane Properties of Archaeal Phospholipids: Effect of Macrocyclization, John Wiley & Sons Ltd., Sussex, England, 1999, 英語, Olivier Dannenmuller, Kenji Arakawa, Tadashi Eguchi, Katsumi Kakinuma, Sylvie Blanc, Anne-Marie Albrecht, Marc Schmutz, Yoichi Nakatani, and Guy Ourisson, pp.385-390

Invited Lecture, Oral Presentation, Poster Presentation

  1. Recent overview of biosynthetic machinery, structural redesign, regulation, and genome mining in Actinomycetes, Kenji Arakawa, The 5th A3 Foresight Symposium on Chemical & Synthetic Biology of Natural Products, 2020/10/30, With Invitation, English, The 5th A3 Foresight Symposium on Chemical & Synthetic Biology of Natural Products, Inha Univ., Korea (Online)
  2. Exploring and functional enhancement of microbial natural products toward healthy-aging society, Kenji Arakawa, International Joint Conference on Science and Technology, Universitas Negeri Makassar, Makassar, Indonesia, 2020/10/16, With Invitation, English, International Joint Conference on Science and Technology, Universitas Negeri Makassar, Makassar, Indonesia, Universitas Negeri Makassar, Makassar, Indonesia (Online)
  3. Microbial natural products discovery through various genome mining approaches, Kenji Arakawa, Kuliah Perdana (Primary Lecture), 2020/03/03, With Invitation, English, University of Jember, Jember, Indonesia, University of Jember, Jember, Indonesia
  4. Concept of natural products discovery and utilization isolated from Sulawesi Island for creating the ecosystem diversity, Kenji Arakawa, Guest Lecture, Universitas Negeri Makassar, Makassar, Indonesia, 2020/02/26, With Invitation, English, Universitas Negeri Makassar, Makassar, Indonesia, Universitas Negeri Makassar, Makassar, Indonesia
  5. Natural products discovery through microbial genome mining approaches and extensive analysis of their biosynthetic machineries and biological activities, Kenji Arakawa, 2019/11/25, With Invitation, English, Heliopolis University, Cairo, Egypt
  6. Overview of biosynthetic machinery, structural redesign, regulatory network, and genome mining in Actinomycetes, Kenji Arakawa, The 4th A3 Foresight Symposium on Chemical & Synthetic Biology of Natural Products, 2019/07/06, With Invitation, English, A3 Foresight Organizer, Shanghai, China
  7. Natural products discovery through microbial genome mining approaches and extensive analysis of their biosynthetic machineries and biological activities, Kenji Arakawa, The Sixth Biology National Seminar, 2019/06/29, With Invitation, English, Universitas Negeri Makassar, Universitas Negeri Makassar, Indonesia
  8. Extensive metabolic engineering for activation of silent secondary metabolite gene clusters in Streptomyces species, Kenji ARAKAWA, 2nd China-Japan Joint Symposium on Natural Product Biosynthesis, 2019/01/14, With Invitation, English, Guang Zhou, China
  9. Microbial genome mining approaches to discover biologically active secondary metabolites, Kenji ARAKAWA, 1st Egyptian-Japanese Joint Symposium; Mining the microbial pathways for the discovery of new bio-active natural products, 2018/12/05, With Invitation, English, Ain Shams University, Cairo, Egypt
  10. Genome Mining, Biosynthesis, and Biological Activity of Azoxyalkene Compound, Kenji ARAKAWA, 1st Germany-Japan Joint Symposium on the Biosynthesis of Natural Products, 2018/09/07, With Invitation, English, Bonn, Germany
  11. Genome-wide metabolic engineering for activation of the silent secondary metabolites gene clusters in Streptomyces species, Kenji Arakawa, The 3rd A3 Foresight Symposium on Chemical & Synthetic Biology of Natural Products, 2018/07/10, With Invitation, English, Sapporo, Japan
  12. Genome mining, biosynthesis, and biological activity of azoxyalkene compound in Streptomyces rochei, Kenji ARAKAWA, Department of Pharmaceutical Sciences Seminars, 2018/03/08, With Invitation, English
  13. Structure, activity and biosynthetic investigation of butenolide-type signaling molecules SRBs that induce antibiotic production in Streptomyces rochei, Kenji Arakawa, 1st China-Japan Joint Symposium on Natural Product Biosynthesis, 2017/10/03, With Invitation, English, Shanghai, China
  14. Genome mining and biosynthetic investigation of azoxyalkene compound produced by a multiple gene disruptant of Streptomyces rochei, Kenji Arakawa, 9th US-Japan Seminar on the Biosynthesis of Natural Products, 2017/06/01, With Invitation, English, Lake Arrowhead, USA
  15. Manipulation of regulatory pathway controlled by signaling molecules SRBs, inducer of antibiotic production in Streptomyces rochei, for genome mining, Kenji Arakawa, 18th International Symposium on the Biology of Actinomycetes, 2017/05/24, With Invitation, English
  16. Biosynthetic investivation of secondary metabolites induced by genome-wide metabolic engineering, Kenji Arakawa, 2nd US-Japan Seminar on the Biosynthesis of Natural Products for Young Researcher, 2017/03/04, With Invitation, English
  17. Biosynthetic investigation of azoxyalkene compound produced by a multiple gene disruptant of Streptomyces rochei, Kenji Arakawa, 2016/07/23, With Invitation, English
  18. Blockage of the early step of lankacidin biosynthesis caused a large production of pentamycin, citreodiol, and epi-citreodiol in Streptomyces rochei, Kenji Arakawa, 17th International Symposium on the Biology of Actinomycetes, 2014/10, Without Invitation, English, 17th International Symposium on the Biology of Actinomycetes, Kusadasi-Aydin, TURKEY
  19. Manipulation of the Streptomyces regulatory network for engineering natural product biosynthesis, 1st US-Japan Seminar on the Biosynthesis of Natural Products for Young Researcher, 2014/03/03, With Invitation, Japanese
  20. Biosynthesis of butenolide signaling molecules that induce lankacidin and lankamycin production in Streptomyces rochei, Kenji Arakawa, 2012/08/25, With Invitation, English, Univ. Shizuoka
  21. Isolation, structural elucidation, and biosynthesis of butenolide signaling molecules that induce lankacidin and lankamycin production in Streptomyces rochei, Kenji Arakawa, International Conference of Natural Products Biosynthesis (ICNPB), 2012/06/19, With Invitation, English, Awaji Shima, JAPAN
  22. Isolation, structural elucidation, and biological activity of the novel gamma-butenolide signaling molecules SRBs that elicit antibiotic production in Streptomyces rochei 7434AN4, Kenji Arakawa, 16th International Symposium on the Biology of Actinomycetes, 2011/12, Without Invitation, English, Puerto Vallarta, MEXICO
  23. Isolation and structural elucidation of the novel -butenolide signaling molecules SRBs that switch on antibiotic production in Streptomyces rochei 7434AN4, International Union of Microbiological Societies 2011 Congress, 2011/09/08, Without Invitation, English, Sapporo, JAPAN
  24. Biosynthesis of the 17-membered polyketide antibiotic lankacidin produced by Streptomyces rochei 7434AN4, Kenji Arakawa, Pacifichem 2010 Congress, 2010/12, With Invitation, English, Honolulu, USA
  25. Mutational analysis of the modular-iterative mixed polyketide biosynthesis of lankacidin, Kenji Arakawa, 15th International Symposium on the Biology of Actinomycetes, 2009/08/21, Without Invitation, English, Shanghai, CHINA
  26. Biosynthesis of the 17-membered polyketide antibiotic lankacidin, Kenji Arakawa, International Symposium of Chemical Biology, 2009/08, With Invitation, English, Xiamen, CHINA
  27. Biosynthetic study of the 17-membered polyketide antibiotic lankacidin, Kenji Arakawa, The 25th Naito Conference "Chemical Biology [II], 2009, Without Invitation, English, Sapporo, JAPAN
  28. Regulation of lankacidin and lankamycin biosynthesis in Streptomyces rochei, Kenji Arakawa, Japan-UK Workshop on Genomics of Antibiotic-producing Actinomycetes: Implications and Applications, 2008/10/31, With Invitation, English, Nihon University Hall, Ichigaya, Tokyo
  29. A modular-iterative mixed polyketide synthases and oxidative macrocyclization in the biosynthesis of the 17-membered antibiotic lankacidin, 7th-Japan-US seminar, 2008/06/23, With Invitation, English, San Diego, USA
  30. Analysis of modular-iterative mixed biosynthesis of lankacidin by heterologous expression and gene fusion, Kenji Arakawa, 14th International Symposium on the Biology of Actinomycetes, 2007/08/27, Without Invitation, English, New Castle, UK
  31. Unique macrocyclization mechanism and iterative type-I PKSs involved in the lankacidin biosynthesis, Kenji Arakawa, Pacifichem 2005 Congress, 2005/12, With Invitation, English, Honolulu, USA
  32. Functions of two cytochrome P-450 hydroxylase genes involved in lankamycin biosynthesis, Kenji Arakawa, Pacifichem 2005 Congress, 2005/12, Without Invitation, English