YASUO UCHIDA

Last Updated :2024/12/02

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Pharmaceutical Sciences), Professor
E-mail
yuchidahiroshima-u.ac.jp
Self-introduction
I was born in Oyama City, Tochigi Prefecture, Japan, and graduated from Oyama Jonan Junior High School and Tochigi High School. I spent my student days at Tohoku University Faculty of Pharmaceutical Sciences, where I worked as an assistant, assistant professor (studied at ETH Zurich as a JSPS overseas fellow for two years), lecturer and associate professor before moving to my current position at the Graduate School of Biomedical and Health Sciences, Hiroshima University in April 2023. I am developing cutting-edge research surrounded by students who are highly motivated in their research. We possess the technology to simultaneously quantify the abundance of thousands of proteins with high accuracy and sensitivity for membrane proteins by quantitative proteomics, which can be applied to a wide variety of samples, including frozen and FFPE human tissues, animals, cells, plants, insects and micro-organisms (The Young Scientists' Award, The Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology). We aim to open up the next generation of pharmaceutics called 'Molecular Systems Pharmaceutics', and to elucidate the mysteries of the brain, brain activity and the molecular mechanisms of human central nervous system disorders, as well as drug discovery for those disorders. We are currently recruiting staff and graduate students, so please feel free to contact us. E-mail, yuchida@hiroshima-u.ac.jp

Basic Information

Major Professional Backgrounds

  • 2023/04/01, Hiroshima University, Graduate School of Biomedical and Health Sciences, Professor
  • 2023/03/01, 2023/03/31, Tohoku University, Graduate School of Pharmaceutical Sciences, Associate Professor
  • 2018/09/01, 2023/02/28, Tohoku University, Graduate School of Pharmaceutical Sciences, Lecturer
  • 2014/04/01, 2016/03/31, ETH Zurich, Institute of Molecular Systems Biology (IMSB), Postdoctral Fellowship for Research Abroad
  • 2012/02/01, 2018/08/31, Tohoku University, Graduate School of Pharmaceutical Sciences, Assistant Professor
  • 2010/04/01, 2012/01/31, Tohoku University, Graduate School of Pharmaceutical Sciences, Research Associate
  • 2008/04/01, 2010/03/31, Tohoku University, Graduate School of Pharmaceutical Sciences, JSPS Research Fellowship for Young Scientists (DC1) (Ph.D. course student)

Educational Backgrounds

  • Tohoku University, Graduate School of Pharmaceutical Sciences, Ph.D. degree, Japan, 2012/01/18
  • Tohoku University, Graduate School of Pharmaceutical Sciences, Ph.D. course, Japan, 2008/04/01, 2010/03/31
  • Tohoku University, Graduate School of Pharmaceutical Sciences, Master course, Japan, 2006/04/01, 2008/03/31
  • Tohoku University, Faculty of Pharmaceutical Sciences, Japan, 2002/04/01, 2006/03/31
  • Tochigi High School, 1999/04/01, 2002/03/31
  • Oyama Jounan Junior High School, 1996/04/01, 1999/03/31

Academic Degrees

  • TOHOKU UNIVERSITY
  • TOHOKU UNIVERSITY

Research Fields

  • Medicine,dentistry, and pharmacy;Pharmacy;Medical pharmacy
  • Complex systems;Biomedical engineering;Biomedical engineering / Biomaterial science and engineering
  • Medicine,dentistry, and pharmacy;Basic medicine;Experimental pathology
  • Medicine,dentistry, and pharmacy;Pharmacy;Pharmacology in pharmacy

Research Keywords

  • Quantitative proteomics of a variety of species including human tissue and membrane proteins (Simultaneous quantification of thousands of proteins)
  • The studies using human brain and spinal cord
  • Barrier regulation
  • Barrier systems biology
  • Central nervous system barrier drug discovery
  • Pharmaceutics
  • Pharmacokinetics

Affiliated Academic Societies

  • The Pharmaceutical Society of Japan
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  • The Japanese Society for the Study of Xenobiotics
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  • The Academy of Pharmaceutical Science and Technology, Japan
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  • International Society for Neurochemistry (Handling Editor of Journal of Neurochemistry)
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  • International Brain Barrier Society
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  • Human Proteome Organization
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  • International Society of Study on Xenobiotics
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  • American Association of Pharmaceutical Scientists
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  • Japan Transporter Research Association
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Educational Activity

Course in Charge

  1. 2024, Undergraduate Education, 2Term, Functional Morphology
  2. 2024, Undergraduate Education, 3Term, Biopharmaceutics
  3. 2024, Undergraduate Education, First Semester, Research Practice A
  4. 2024, Undergraduate Education, Second Semester, Research Practice B
  5. 2024, Undergraduate Education, First Semester, Practice of Drug Delivery System
  6. 2024, Undergraduate Education, 1Term, Pharmacokinetics
  7. 2024, Undergraduate Education, Intensive, Pharmacokinetics
  8. 2024, Undergraduate Education, 3Term, Japanese Pharmacopoeia
  9. 2024, Undergraduate Education, First Semester, Practice of Pharmaceutics
  10. 2024, Undergraduate Education, Year, Special laboratory Works in Pharmaceutical Sciences I
  11. 2024, Undergraduate Education, Year, Special laboratory Works in Pharmaceutical Sciences II
  12. 2024, Undergraduate Education, Second Semester, Special laboratory Works in Pharmaceutical Sciences III
  13. 2024, Undergraduate Education, Year, Special laboratory Works in Clinical Pharmacy I
  14. 2024, Undergraduate Education, Year, Special laboratory Works in Clinical Pharmacy II
  15. 2024, Undergraduate Education, Year, Special laboratory Works in Clinical Pharmacy III
  16. 2024, Liberal Arts Education Program1, 1Term, Introductory Seminar for First-Year Students
  17. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Molecular Systems Pharmaceutics
  18. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Molecular Systems Pharmaceutics
  19. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Molecular Systems Pharmaceutics
  20. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Molecular Systems Pharmaceutics
  21. 2024, Graduate Education (Master's Program) , First Semester, Lecture on Medicinal Science
  22. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Lecture on Medicinal Science
  23. 2024, Graduate Education (Master's Program) , First Semester, Seminar on Drug Delivery Sciences
  24. 2024, Graduate Education (Master's Program) , Second Semester, Seminar on Drug Delivery Sciences
  25. 2024, Graduate Education (Master's Program) , First Semester, Seminar on Molecular Systems Pharmaceutics
  26. 2024, Graduate Education (Master's Program) , Second Semester, Seminar on Molecular Systems Pharmaceutics
  27. 2024, Graduate Education (Master's Program) , First Semester, Advanced Research on Molecular Systems Pharmaceutics
  28. 2024, Graduate Education (Master's Program) , Second Semester, Advanced Research on Molecular Systems Pharmaceutics
  29. 2024, Graduate Education (Master's Program) , 3Term, Lecutre on Molecular Systems Pharmaceutics
  30. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Molecular Systems Pharmaceutics
  31. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Molecular Systems Pharmaceutics
  32. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Molecular Systems Pharmaceutics
  33. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Molecular Systems Pharmaceutics

Research Activities

Academic Papers

  1. SWATH-MS-based functional analysis of metal transporters, Seitainokagaku, 75(2), 116-121, 2024
  2. Blood-Arachnoid Barrier: A new central nervous system barrier having different transport molecular mechanisms from blood-brain barrier, Membrane, 49(2), 99-106, 2024
  3. Abemaciclib-induced epithelial-mesenchymal transition mediated by cyclin-dependent kinase 4/6 independent of cell cycle arrest pathway, Int J Biochem Cell Biol, 172, 106601, 2024
  4. Transporter Protein Expression of Corneal Epithelium in Rabbit and Porcine: Evaluation of Models for Ocular Drug Transport Study, Mol Pharm, 2024
  5. ZNT5-6 and ZNT7 play an integral role in protein N-glycosylation by supplying Zn to Golgi alpha-mannosidase II, J Biol Chem, 300(6), 107378, 2024
  6. The Effects of N-Glycosylation on the Expression and Transport Activity of OATP1A2 and OATP2B1, J Pharm Sci, 113(5), 1376-1384, 2024
  7. Activation of prefrontal parvalbumin interneurons ameliorates working memory deficit even under clinically comparable antipsychotic treatment in a mouse model of schizophrenia, Neuropsychopharmacology, 49(4), 720-730, 2024
  8. Aggregability of the SQSTM1/p62-based aggresome-like induced structures determines the sensitivity to parthanatos, CELL DEATH DISCOVERY, 10(1), 20240212
    Link to Paper Search Results by DOI
  9. Next generation quantitative proteomics-based central nervous system barrier transporter studies, Pharmaceutics, 83, 72-77, 2023
  10. Characterization of proteome profile data of chemicals based on data-independent acquisition MS with SWATH method, NAR Genom Bioinform, 5, lqad022, 2023
  11. Design and Synthesis of Orexin 1 Receptor-Selective Agonists, J Med Chem, 66, 5453-5464, 2023
  12. COL1A2 inhibition suppresses glioblastoma cell proliferation and invasion, J Neurosurg, 138, 639-648, 2023
  13. The molecules and their functions involved in transport, Clinical Neuroscience, 40(12), 1531-1534, 2022
  14. ★, Blood-arachnoid barrier as a dynamic physiological and pharmacological interface between cerebrospinal fluid and blood, Drug Delivery to the Brain, 93-121, 2022
  15. Pharmacoproteomics of Brain Barrier Transporters and Substrate Design for the Brain Targeted Drug Delivery, Pharm Res, 39, 1363-1392, 2022
  16. Quantitative Targeted Absolute Proteomics for Better Characterization of an In Vitro Human Blood-Brain Barrier Model Derived from Hematopoietic Stem Cells, Cells, 11, 3963, 2022
  17. Proteomics-Based Transporter Identification by the PICK Method: Involvement of TM7SF3 and LHFPL6 in Proton-Coupled Organic Cation Antiport at the Blood-Brain Barrier, Pharmaceutics, 14, 1683, 2022
  18. ★, ssPalm-based lipid nanoparticles achieve efficient mRNA transfection in brain capillary endothelial cells, Pharmaceutics, 14, 1560, 2022
  19. Discovery of novel orexin receptor antagonists using a 1,3,5-trioxazatriquinane bearing multiple effective residues (TriMER) library, Eur J Med Chem, 240, 114505, 2022
  20. ★, Proteomics-based investigation of cerebrovascular molecular mechanisms in cerebral amyloid angiopathy by the FFPE-LMD-PCT-SWATH method, Fluids Barriers CNS, 19, 56, 2022
  21. Upregulation of ribosome complexes at the blood-brain barrier in Alzheimer's disease patients, J Cereb Blood Flow Metab, 42, 2134-2150, 2022
  22. Zinc transport via ZNT5-6 and ZNT7 is critical for cell surface glycosylphosphatidylinositol-anchored protein expression, J Biol Chem, 298, 102011, 2022
  23. Regional Differences in the Absolute Abundance of Transporters, Receptors and Tight Junction Molecules at the Blood-Arachnoid Barrier and Blood-Spinal Cord Barrier among Cervical, Thoracic and Lumbar Spines in Dogs, Pharm Res, 39, 1393-1413, 2022
  24. ★, A human blood-arachnoid barrier atlas of transporters, receptors, enzymes, and tight junction and marker proteins: Comparison with dog and pig in absolute abundance, J Neurochem, 161, 187-208, 2022
  25. SWATH-Based Comprehensive Determination of the Localization of Apical and Basolateral Membrane Proteins Using Mouse Liver as a Model Tissue, Biomedicines, 10, 383, 2022
  26. Activation of Annexin A2 signaling at the blood-brain barrier in a mouse model of multiple sclerosis, J Neurochem, 160, 662-674, 2022
  27. Identification and Validation of Combination Plasma Biomarker of Afamin, Fibronectin and Sex Hormone-Binding Globulin to Predict Pre-Eclampsia, Biol Pharm Bull, 44, 804-815, 2021
  28. An Atlas of the Quantitative Protein Expression of Anti- Epileptic-Drug Transporters, Metabolizing Enzymes and Tight Junctions at the Blood-Brain Barrier in Epileptic Patients, Pharmaceutics, 13, 2122, 2021

Invited Lecture, Oral Presentation, Poster Presentation

  1. Quantitative proteomics using paraffin sections: how to achieve efficient protein extraction, Yasuo Uchida, JPrOS2024 & JSCP20th, 2024/06/26, With Invitation, Japanese, Aomori
  2. Quantitative proteomic studies of the central nervous system barrier and brain pathology targeting neurological disease treatment, Yasuo Uchida, The 39th Annual Meeting of the Academy of Pharmaceutical Science and Technology, Japan (APSTJ), 2024/05/23, With Invitation, Japanese, Kobe
  3. Pathological proteomics research using human brain tissue from the Brain Bank, the pride of Japan, Yasuo Uchida, The 144th Annual Meeting of the Pharmaceutical Society of Japan, 2024/03/28, With Invitation, Japanese, Yokohama
  4. Next Generation Quantitative Proteomics Opens Up New Dimensions in Central Nervous System Barrier Research, Yasuo Uchida, The 149th Regular Meeting of Chugoku-Shikoku Branch of the Japanese Pharmaceutical Society, 2024/01/27, With Invitation, Japanese, Hiroshima
  5. Quantitative proteomics-based drug transport studies, Yasuo Uchida, Drug Discovery Pharmacokinetics Forum in Kanazawa, 2024/01/15, With Invitation, Japanese, Kanazawa University
  6. Quantitative membrane protein proteomics to elucidate the molecular mechanisms of transport in central tissues, Yasuo Uchida, 2023 Annual conference of the Membrane Society of Japan, 2023/11/20, With Invitation, Japanese, Tokyo
  7. Next generation quantitative proteomics opens up the new fields of central nervous system barrier studies, Yasuo Uchida, Korean Society of Applied Pharmacology (KSAP) annual meeting 2023, 2023/10/06, With Invitation, English, Korea
  8. The next generation of CNS disease treatment pioneered by the latest protein research, Yasuo Uchida, R5 Hiroshima Pharmacist Seminar, 2023/07/30, With Invitation, Japanese, Hiroshima
  9. Central nervous system barrier quantitative proteomics using human clinical specimens, Yasuo Uchida, JPrOS2023(21st JHUPO) Annual Meeting, 2023/07/24, With Invitation, Japanese, Niigata
  10. Metal traffic research using next-generation quantitative proteomics, Yasuo Uchida, The 23rd Annual Meeting of the Protein Science Society of Japan, 2023/07/05, With Invitation, Japanese, Nagoya
  11. Next generation quantitative proteomics opens up the new fields of CNS barrier studies, Yasuo Uchida, CVB2023 conference, 2023/06/18, With Invitation, English, Uppsala, Sweden
  12. Drug transporter studies in the central nervous system barrier using human postmortem brains, Yasuo Uchida, 64th Annual Meeting of the Japanese Society of Neurology, 2023/05/31, With Invitation, Japanese, Tokyo
  13. Proteomic measurements of the neurovascular units surrounding astrocytes, Yasuo Uchida, 34th Annual Clinical MR Brain Function Research Conference, 2023/05/13, With Invitation, Japanese, Tokyo
  14. Quantitative proteomics research for the effective use of human postmortem brains, Yasuo Uchida, The 112th Annual Meeting of the Japanese Society of Pathology, 2023/04/13, With Invitation, Japanese, Shimonoseki
  15. Next-generation quantitative proteomics opens up new horizons in CNS research, Y Uchida, 6th Mass Spectrometry Society of Japan, 2023/03/30, With Invitation, Japanese, Sendai
  16. Mechanisms of brain-selective migration of exosomes from metastatic melanoma in the brain, Y Uchida, 2022 conference of extracellular fine particles, 2023/01/31, Without Invitation, Japanese, Kumamoto
  17. New horizons in CNS research opened up by next-generation quantitative proteomics, Y Uchida, Brain Molecular Exploration seminar, 2022/12/26, With Invitation, Japanese, Tokyo
  18. Discovery for new substrates for MFSD10, a transporter highly expressed in the blood-brain barrier, Rhalib reda Akagawa, Tetsu Sato, Taiho Kambe, Yasuo Uchida, 61st Annual Meeting of the Tohoku Branch of the Pharmaceutical Society of Japan, 2022/11/27, Without Invitation, Japanese, online
  19. Blood-brain barrier MFSD10 limits drug brain migration and central effects of drugs, Tetsu Sato, Rhalib reda Akagawa, Yasuo Uchida, 61st Annual Meeting of the Tohoku Branch of the Pharmaceutical Society of Japan, 2022/11/27, Without Invitation, Japanese, online
  20. Next-generation proteomics opens up new central nervous system barrier, Y Uchida, 21st Seminar for Young Researchers in Medical Pharmacy, 2022/11/12, With Invitation, Japanese, Sendai
  21. Bottom-up construction of brain-shaped nanoparticles for a breakthrough in the delivery of polymeric medicines to the brain, Y Uchida, 2nd meeting of bottom-up biotech, 2022/09/26, Without Invitation, Japanese, Rusutsu
  22. Report on collaborative research based on the quantitative proteomics SWATH method, Y Uchida, 2022 conference of biometal science, 2022/09/03, Without Invitation, Japanese, Rusutsu
  23. Central nervous system barrier research using quantitative proteomics, Y Uchida, 5th conference of extracellular fine particles, 2022/07/21, Without Invitation, Japanese, online
  24. Next generation quantitative proteomics and its application to metal transport study, Y Uchida, The 22nd Annual Meeting of the Protein Science Society of Japan, 2022/06/09, With Invitation, Japanese, Tsukuba
  25. New central barrier opened up by new technologies, Y Uchida, APSTJ 37th annual meeting, 2022/05/26, With Invitation, Japanese, Kyoto
  26. The role of the blood-brain barrier, blood-arachnoid barrier and blood-cerebrospinal fluid barrier in metal dynamics in the human brain, Y Uchida, 2022 1st meeting of biometal science, 2022/05/21, Without Invitation, Japanese, Tokyo
  27. Establishment of a diagnostic basis for personalised pharmacotherapy of refractory cancers based on reconstruction of the molecular clouding environment of cancer cells, Y Uchida, 6th meeting of molecular clouding chemistry, 2022/01/27, Without Invitation, Japanese, online

Awards

  1. 2023/04/19, 2023 the MEXT Young Scientists' Prize, Ministry of Education, Culture, Sports, Science and Technology, Human central nervous system study based on the development of human tissue quantitative proteomics
  2. 2022/04/01, CREST extracellular fine particle Young Innovator Award, JST CREST

External Funds

Acceptance Results of Competitive Funds

  1. Grants-in-Aid for Scientific Research, Challenging Research (Exploratory), 2024, 2025
  2. Grant-in-Aid for Transformative Research Areas (A), 2024, 2025
  3. Grants-in-Aid for Scientific Research, Scientific Research(A), 2022, 2025
  4. Grants-in-Aid for Scientific Research, Innovative Areas (Research in a proposed research area) Single-year Grants, 2022, 2023
  5. Grants-in-Aid for Scientific Research, Transformative Research Areas (A), 2022, 2023
  6. AMED, Research and Development Grants for Dementia, 2022, 2024
  7. CREST Young Scientist Challenge, 2022, 2022
  8. AMED, Practical Research for Innovative Cancer Control, 2021, 2023
  9. Grants-in-Aid for Scientific Research, Challenging Research (Exploratory), 2021, 2022
  10. Grants-in-Aid for Scientific Research, Scientific Research(B), 2020, 2022
  11. Grants-in-Aid for Scientific Research, Scientific Research(B), 2023, 2025
  12. Grants-in-Aid for Scientific Research, Fund for the Promotion of Joint International Research (Fostering Joint International Research (A)), 2019, 2023