Emiko Kinoshita

Last Updated :2023/06/01

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Pharmaceutical Sciences), Assistant Professor
Web Site
http://phostag.hiroshima-u.ac.jp
E-mail
kikutahiroshima-u.ac.jp
Other Contact Details
KASUMI 1-2-3, HIROSHIMA 734-8551, JAPAN, Japan
TEL : (+81)82-257-5281 FAX : (+81)82-257-5336

Basic Information

Academic Degrees

  • Ph.D., Hiroshima University
  • Master of Pharmaceutical Science, Hiroshima University
  • Bachelor of Pharmaceutical Science, Hiroshima University

Educational Activity

  • [Bachelor Degree Program] School of Pharmaceutical Sciences : Program of Pharmaceutical Sciences : Pharmaceutical Sciences
  • [Bachelor Degree Program] School of Pharmaceutical Sciences : Program of Medicinal Sciences : Medicinal Sciences
  • [Master's Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Medicinal Sciences
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Medicinal Sciences

Research Fields

  • Medicine,dentistry, and pharmacy;Pharmacy;Physical pharmacy

Research Keywords

  • SNP
  • phosphoproteome
  • cyclen
  • phos-tag
  • zinc
  • phosphorylation

Educational Activity

Course in Charge

  1. 2023, Undergraduate Education, 1Term, Pharmaceutical Physical Chemistry
  2. 2023, Undergraduate Education, 3Term, Japanese Pharmacopoeia
  3. 2023, Undergraduate Education, 2Term, Biophysical Chemistry
  4. 2023, Undergraduate Education, Second Semester, Training of Physical Chemistry
  5. 2023, Graduate Education (Master's Program) , 1Term, Methods in Biomedical Sciences A
  6. 2023, Graduate Education (Doctoral Program) , 1Term, Methods in Biomedical Sciences B

Research Activities

Academic Papers

  1. Evaluation of four phosphopeptide enrichment strategies for mass spectrometry-based proteomic analysis, PROTEOMICS, 22(7), 202204
    Link to Paper Search Results by DOI
  2. Characterization of the Binding of Adenosine-5-monophosphate to a m-Type Alkoxide-linked Dinuclear Zinc(II) Complex in Crystal and Solution State, Bull. Chemi. Soc. Jap., 2021
  3. Characterization of phosphorylation status and kinase activity of Src family kinases expressed in cell-based and cell-free protein expression systems, Biomolecules, 11, 1448, 20211002
  4. An assay of human tyrosine protein kinase ABL activity using an Escherichia coli protein expression system, BIOTECHNIQUES, 70(4), 209-217, 202104
    Link to Paper Search Results by DOI
  5. Phos-tag-based micropipette-tip method for analysis of phosphomonoester-type impurities in synthetic oligonucleotides., J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 1151, 122198, 2020
  6. An immuno-dot blot assay for screening histidine kinase inhibitors, ANALYTICAL BIOCHEMISTRY, 600, 20200701
    Link to Paper Search Results by DOI
  7. Phos-tag-based micropipette-tip method for analysis of phosphomonoester-type impurities in synthetic oligonucleotides, JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1151, 20200815
    Link to Paper Search Results by DOI
  8. ★, Ptorein-N-myristylation-dependent phosphorylation of serine 13 or tyrosine kinase Lyn by casein kinase 1gamma at the Golgi during intracellular protein traffic, Scientific Report, 10, 16273, 2020
  9. Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1 gamma at the Golgi during intracellular protein traffic, SCIENTIFIC REPORTS, 10(1), 20201001
    Link to Paper Search Results by DOI
  10. Increase in constitutively active MEK1 species by introduction of MEK1 mutations identified in cancers, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1867(1), 62-70, 201901
    Link to Paper Search Results by DOI
  11. A method for profiling the phosphorylation state of tyrosine protein kinases, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1867(1), 71-75, 201901
    Link to Paper Search Results by DOI
  12. Gel-based analysis of protein phosphorylation status by rapid fluorometric staining using TAMRA-labeled Phos-tag., Journal of Electrophoresis, 63, 25-32, 2019
  13. Quantitative monitoring of His and Asp phosphorylation in a bacterial signaling system by using Phos-tag Magenta/Cyan fluorescent dyes, Electrophoresis, 2019
  14. Quantitative monitoring of His and Asp phosphorylation in a bacterial signaling system by using Phos-tag Magenta/Cyan fluorescent dyes, ELECTROPHORESIS, 40(22), 3005-3013, 201911
    Link to Paper Search Results by DOI
  15. A strategy to identify protein-N-myristoylation-dependent phosphorylation reactions of cellular proteins by using Phostag SDS-PAGE, PLOS ONE, 14(11), 20191121
    Link to Paper Search Results by DOI
  16. A simple method for determining the ligand affinity toward a zinc-enzyme model by using a TAMRA/TAMRA interaction, DALTON TRANSACTIONS, 47(6), 1841-1848, 20180214
    Link to Paper Search Results by DOI
  17. Increase in constitutively active MEK1 species by introduction of MEK1 mutations identified in cancers, Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2018
  18. A method for profiling the phosphorylation state of tyrosine protein kinases, Biochimica et Biophysica Acta (BBA) - Proteins and Proteomic, 2018
  19. A simple method for determining the ligand affinity toward a zinc-enzyme, Dalton transaction, 47, 1840-1848, 2018
  20. Specific glutamic acid residues in targeted proteins induce exaggerated retardations in Phos-tag SDS-PAGE migration, ELECTROPHORESIS, 38(8), 1139-1146, 201704
    Link to Paper Search Results by DOI
  21. TAMRA/TAMRA Fluorescence Quenching Systems for the Activity Assay of Alkaline Phosphatase., Sensors, 17(8), 1877-1-1877-12, 201708
  22. A Phos-tag-based micropipette-tip method for rapid and selective enrichment of phosphopeptides, ELECTROPHORESIS, 38(19), 2447-2455, 201710
    Link to Paper Search Results by DOI
  23. Zn(II)–Phos-tag SDS-PAGE for Separation and Detection of a DNA Damage-Related Signaling Large Phosphoprotein, ATM Kinase: Methods and Protocols, Methods in Molecular Biology, 1599(1599), 113-126, 2017
  24. Phos-tag SDS-PAGE methodology that effectively uses phosphoproteomic data for profiling the phosphorylation dynamics of MEK1, Journal of electrophoresis, 61, 9-15, 2017
  25. Validation of Cis and Trans Modes in Multistep Phosphotransfer Signaling of Bacterial Tripartite Sensor Kinases by Using Phos-Tag SDS-PAGE, PLOS ONE, 11(2), e0148294, 20160201
    Link to Paper Search Results by DOI
  26. A Phos-tag SDS-PAGE method that effectively uses phosphoproteomic data for profiling the phosphorylation dynamics of MEK1, PROTEOMICS, 16(13), 1825-1836, 201607
    Link to Paper Search Results by DOI
  27. A novel thiol-affinity micropipette tip method using zinc(II)-cyclen-attached agarose beads for enrichment of cysteine-containing molecules, JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1031, 195-201, 20160915
    Link to Paper Search Results by DOI
  28. Advances in Phos-tag-based methodologies for separation and detection of the phosphoproteome, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1854(6), 601-608, 201506
    Link to Paper Search Results by DOI
  29. Functional Characterization of the Receiver Domain for Phosphorelay Control in Hybrid Sensor Kinases, PLOS ONE, 10(7), e0132598-e0132598, 201507
    Link to Paper Search Results by DOI
  30. Improving the Electrotransfer Efficiency of Target Phosphoprotein from Phos-tag SDS-PAGE Gel, BUNSEKI KAGAKU, 64(7), 501-509, 201507
  31. The cutting edge of affinity electrophoresis technology, Proteomes, 3, 42-55, 2015
  32. Neutral phosphate affinity SDS-PAGE system for profiling of protein phosphorylation, Methods in Molecular Biology, 1295, 323-354, 2015
  33. Expression and phosphorylation state analysis of intracellular protein kinases using Multi-PK antibody and Phos-tag SDS-PAGE, METHODSX, 2, 469-474, 2015
    Link to Paper Search Results by DOI
  34. The Cutting Edge of Affinity Electrophoresis Technology, PROTEOMES, 3(1), 42-55, 201503
    Link to Paper Search Results by DOI
  35. Profiling of protein thiophosphorylation by Phos-tag affinity electrophoresis: Evaluation of adenosine 5-O-( 3-thiotriphosphate) as a phosphoryl donor in protein kinase reactions, PROTEOMICS, 14(6), 668-679, 201403
    Link to Paper Search Results by DOI
  36. Simple enrichment of thiol-containing biomolecules by using zinc(II)-cyclen-functionalized magnetic beads, JOURNAL OF SEPARATION SCIENCE, 37(13), 1601-1609, 201407
    Link to Paper Search Results by DOI
  37. Tips on improving the efficiency of electrotransfer of target proteins from Phos-tag SDS-PAGE gel, PROTEOMICS, 14(21-22), 2437-2442, 201411
    Link to Paper Search Results by DOI
  38. A Phos-tag-based fluorescence quenching system for activity assay and inhibitor screening for alkaline phosphatase., Americam Journal of Analitycal Chemistry, 5, 796-804, 2014
  39. アフィニティー電気泳動技術の最前線, ぶんせき, 7, 365-371, 2014
  40. Identification of two phosphorylated species of β-catenin involved in the ubiquitin-proteasomepathway by using two- dimensional Phos-tag affinity electrophoresis., Journal of Electrophoresis, 58, 1-4, 2014
  41. A Phos-tag-based magnetic-bead method for rapid and selective separation of phosphorylated biomolecules, JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 925, 86-94, 20130415
    Link to Paper Search Results by DOI
  42. Sandwich assay for phosphorylation of protein multiplexes by using antibodies and Phos-tag, ANALYTICAL BIOCHEMISTRY, 438(2), 104-106, 20130715
    Link to Paper Search Results by DOI
  43. Increase in Lipophilicity of Enalaprilat by Complexation with Copper (II) or Zinc (II) Ions, YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 133(10), 1135-1141, 201310
    Link to Paper Search Results by DOI
  44. Highly sensitive detection method for protein phosphorylation and its development as a new technique toward phosphoproteomics, 85(6), 447-455, 20130601
  45. Phos-tag-Based Microarray Techniques Advance Phosphoproteomics, Journal of Proteomics & Bioinformatics, S6(1), 8, 20130601
  46. Phos-tag SDS-PAGE systems for phosphorylation profiling of proteins with a wide range of molecular masses under neutral pH conditions, PROTEOMICS, 12(2), 192-202, 201201
    Link to Paper Search Results by DOI
  47. Highly sensitive detection of protein phosphorylation by using improved Phos-tag Biotin, PROTEOMICS, 12(7), 932-937, 201204
    Link to Paper Search Results by DOI
  48. Separation and identification of four distinct serine-phosphorylation states of ovalbumin by Phos-tag affinity electrophoresis, ELECTROPHORESIS, 33(5), 849-855, 201203
    Link to Paper Search Results by DOI
  49. Phos-tag Technology for Phosphoproteomics, BUNSEKI KAGAKU, 61(6), 469-487, 201206
    Link to Paper Search Results by DOI
  50. A Laborsaving, Timesaving, and More Reliable Strategy for Separation of Low-Molecular-Mass Phosphoproteins in Phos-tag Affinity Electrophoresis, Intrenational Journal of Chemistry, 4(5), e1-e8, 20120901
  51. Protocols for the analysis of phosphoproteins using Phos-tag technology, 56, s51-s75, 20120101
  52. Phso-tag-based fluorescence resonance energy transfer system for the analysis of protein kinase and phosphatase reactions, 56, s45-s49, 20120101
  53. Improved Phos-tag SDS-PAGE under neutral pH conditions for advanced profiling of protein phosphorylation, 56, s41-s44, 20120101
  54. Phos-tag chemistry, 56, s3-s7, 20120101
  55. Phos-tag affinity electrophoresis for protein kinase profiling, in Protein Kinase Technology (ed. by Mukai, H), Neuromethods (Springer, New York), 68, 13-34, 20120601
  56. Zinc(II)-cyclen polyacrylamide gel electrophoresis for detection of mutations in short Ade/Thy-rich DNA fragments, ANALYTICAL BIOCHEMISTRY, 408(2), 348-350, 20110115
    Link to Paper Search Results by DOI
  57. Improved Phos-tag SDS-PAGE under neutral pH conditions for advanced protein phosphorylation profiling, PROTEOMICS, 11(2), 319-323, 201101
    Link to Paper Search Results by DOI
  58. A Phos-tag-based fluorescence resonance energy transfer system for the analysis of the kinase reaction of a substrate peptide, ANALYTICAL METHODS, 3(6), 1303-1309, 201106
    Link to Paper Search Results by DOI
  59. A novel phosphate-affinity bead immobilized with Phos-tag for separation and enrichment of phosphopeptides and phosphoproteins, Journal of Integrated Omics, 1(1), 157-169, 20110201
  60. Genotyping and mapping assay of single-nucleotide polymorphisms in CYP3A5 using DNA-binding zinc(II) complexes, CLINICAL BIOCHEMISTRY, 43(3), 302-306, 201002
    Link to Paper Search Results by DOI
  61. The DNA-binding activity of mouse DNA methyltransferase 1 is regulated by phosphorylation with casein kinase 1 delta/epsilon, BIOCHEMICAL JOURNAL, 427, 489-497, 20100501
    Link to Paper Search Results by DOI
  62. Monitoring method for the alteration of phosphorylation status in a high-molecular-mass protein using Phos-tag electrophoresis technology, 82(9), 857-862, 20100901
  63. Two-dimensional phosphate-affinity gel electrophoresis for the analysis of phosphoprotein isotypes, ELECTROPHORESIS, 30(3), 550-559, 200902
    Link to Paper Search Results by DOI
  64. A Phos-tag-based fluorescence resonance energy transfer system for the analysis of the dephosphorylation of phosphopeptides, ANALYTICAL BIOCHEMISTRY, 388(2), 235-241, 20090515
    Link to Paper Search Results by DOI
  65. Mobility shift detection of phosphorylation on large proteins using a Phos-tag SDS-PAGE gel strengthened with agarose, PROTEOMICS, 9(16), 4098-4101, 200908
    Link to Paper Search Results by DOI
  66. Separation and detection of large phosphoproteins using Phos-tag SDS-PAGE, NATURE PROTOCOLS, 4(10), 1513-1521, 2009
    Link to Paper Search Results by DOI
  67. Phos-tag beads as an immunoblotting enhancer for selective detection of phosphoproteins in cell lysates, Analytical Biochemistry, 389(1), 83-85, 20090601
  68. Phosphate-affinity Gel Electrophoresis Using a Phos-tag Molecule for Phosphoproteome Study, Current Proteomics, 6, 104-121, 20090701
  69. Phosphate-affinity polyacrylamide gel electrophoresis for SNP genotyping, in Single Nucleotide Polymorphisms (ed. by Komar AA), Methods in Molecular Biology (Humana Press), 578, 183-192, 20091101
  70. Zn(II)-cyclen polyacrylamide gel electrophoresis for SNP detection, in Single Nucleotide Polymorphisms (ed. by Komar AA), Methods in Molecular Biology (Humana Press), 578, 169-182, 20091101
  71. Phosphate-Affinity Gel Electrophoresis Using a Phos-Tag Molecule for Phosphoproteome Study, CURRENT PROTEOMICS, 6(2), 104-121, 200907
    Link to Paper Search Results by DOI
  72. A mobility shift detection method for DNA methylation analysis using phosphate affinity polyacrylamide gel electrophoresis, ANALYTICAL BIOCHEMISTRY, 378(1), 102-104, 20080701
    Link to Paper Search Results by DOI
  73. Detection of the Gua/Cyt-to-Cyt/Gua mutation in a Gua/Cyt-lined sequence using Zn2+-cyclen polyacrylamide gel electrophoresis, ANALYTICAL BIOCHEMISTRY, 380(1), 122-127, 20080901
    Link to Paper Search Results by DOI
  74. Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE, PROTEOMICS, 8(15), 2994-3003, 200808
    Link to Paper Search Results by DOI
  75. FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway, NATURE STRUCTURAL & MOLECULAR BIOLOGY, 15(11), 1138-1146, 200811
    Link to Paper Search Results by DOI
  76. Specific recognition and detection of phosphorylated proteins using characteristics of metal ion, YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 127(12), 1897-1913, 200712
    Link to Paper Search Results by DOI
  77. Non-SCN5A Related Brugada Syndromes: Verification of Normal Splicing and Trafficking of SCN5A without Exonic Mutations, Annals of Human Genetics, 71, 8-17, 20070101
  78. Separation of a phosphorylated-His protein using phosphate-affinity polyacrylamide gel electrophoresis, Analytical Biochemistry, 360, 160-162, 20070101
  79. Label-free kinase profiling using phosphate affinity polyacrylamide gel electrophoresis, 6(2), 356-366, 20070201
  80. Identification on membrane and characterization of phosphoproteins using an alkoxide-bridged dinuclear metal complex as a phosphate binding tag molecule, Journal of Biomolecular Techniques, 18(5), 278-286, 20071201
  81. A single nucleotide polymorphism genotyping method using phosphate-affinity polyacrylamide gel electrophoresis, Analytical Biochemistry, 361, 294-298, 20070201
  82. Novel type of phosphate-affinity electrophoresis for protein kinase profiling, 51(3), 199-206, 20070901
  83. Phosphate-binding tag, a new tool to visualize phosphorylated proteins, MOLECULAR & CELLULAR PROTEOMICS, 5(4), 749-757, 200604
    Link to Paper Search Results by DOI
  84. Enrichment of phosphorylated proteins from cell lysate using a novel phosphate-affinity chromatography at physiological pH, PROTEOMICS, 6(19), 5088-5095, 200610
    Link to Paper Search Results by DOI
  85. A heteroduplex-preferential T-m depressor for the specificity-enhanced DNA polymerase chain reactions, ANALYTICAL BIOCHEMISTRY, 337(1), 154-160, 20050201
    Link to Paper Search Results by DOI
  86. Novel immobilized zinc(II) affinity chromatography for phosphopeptides and phosphorylated proteins, JOURNAL OF SEPARATION SCIENCE, 28(2), 155-162, 200502
    Link to Paper Search Results by DOI
  87. An alkoxide-bridged dinuclear zinc(II) hexaazacryptate: A novel phosphate capture molecule in aqueous solution, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 78(1), 125-131, 20050115
    Link to Paper Search Results by DOI
  88. Reliable and cost-effective screening of inherited heterozygosity by Zn2+-cyclen polyacrylamide gel electrophoresis, CLINICAL CHEMISTRY, 51(11), 2195-2198, 200511
    Link to Paper Search Results by DOI
  89. A macrocyclic zinc(II) fluorophore as a detector of apoptosis, Proceedings of the National Academy of Sciences of USA, 100(7), 3731-3736, 20030401
  90. A novel procedure for simple and efficient genotyping of single nucleotide polymorphisms by using the Zn2+-cyclen complex, NUCLEIC ACIDS RESEARCH, 30(22), 20021115
    Link to Paper Search Results by DOI
  91. New potent agents binding to a poly(dT) sequence in double-stranded DNA: bis(Zn2+?cyclen) and tris (Zn2+?cyclen) complexes, Journal of Biological Inorganic Chemistry, 7, 473-482, 20020101
  92. A new type of potent inhibitors of HIV-1 TAR RNA-Tat peptide binding by zinc(II)-macrocyclic tetraamine complexes, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 123(32), 7911-7912, 20010815
    Link to Paper Search Results by DOI
  93. Selective recognition of consecutive G sequence in double-stranded DNA by a Zinc(II)-macrocyclic tetraamine complex appended with an anthraquinone, Journal of Inorganic Biochemistry, 82, 239-249, 20001001
  94. Controlling gene expression by zinc(II)-macrocyclic tetraamine complexes, Journal of Inorganic Biochemistry, 79, 253-259, 20000101
  95. Natural and synthetic double-stranded DNA binding studies of macrocyclic tetraamine complexes., Journal of biological inorganic chemistry, 4, 431-440, 19990801
  96. A new type of potent inhibitors of HIV-1 TAR RNA-Tat peptide binding by zinc(II)?macrocyclic tetraamine complexes, Journal of the American Chemical Society, 123, 7911-7912, 19990901
  97. Novel Recognition of Thymine Base in Double-Stranded DNA by Zinc(II)-Macrocyclic Tetraamine Complexes Appended with Aromatic Groups, Journal of American Chemical Society, 121(23), 5426-5436, 19990701
  98. Characterization of Phosphorylation Status and Kinase Activity of Src Family Kinases Expressed in Cell-Based and Cell-Free Protein Expression Systems, BIOMOLECULES, 11(10), 202110
    Link to Paper Search Results by DOI
  99. History of Phos-tag technology for phosphoproteomics, JOURNAL OF PROTEOMICS, 252, 20220210
    Link to Paper Search Results by DOI
  100. Recent advances in the Phos-tag technique focused on the analysis of phosphoproteins in a bacterial two-component system, JOURNAL OF PROTEOMICS, 252, 20220210
    Link to Paper Search Results by DOI
  101. Crystal Structure of Bis{1,3-bis[bis(pyridin-2-ylmethyl)amino]propan-2-olato-dizinc(II)}orthophosphate Tris(perchlorate) Octahydrate, [(Phos-tag)(2)-PO43-][ClO4-](3)center dot 8H(2)O, X-RAY STRUCTURE ANALYSIS ONLINE, 37, 87-88, 20211210
  102. Characterization of the Binding of Adenosine-5 '-monophosphate to a mu-Type Alkoxide-Linked Dinuclear Zinc(II) Complex in Crystal and Solution State, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 94(11), 2670-2677, 202111
    Link to Paper Search Results by DOI

Publications such as books

  1. 2021, Methods in Molecular Biology, Determining protein phosphorylation status using antibody arrays and Phos-tag biotin., Springer, New York, 2021, Joint work, Kinoshita E, Kinoshita-Kikuta E., Koike T.
  2. 2021, Methods in Molecular Biology, Phos-Tag Fluorescent Gel Staining for the Quantitative Detectionof His- and Asp-Phosphorylated Proteins, Springer, New York, 2021, Scholarly Book, Joint work, Kinoshita-Kikuta E., Kinoshita E. Koike T.
  3. Neuroproteomics: Neuromthods, (2019) 146 (ed. by Li, KW) Springer75-84, Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag Tip., 2019, Joint work, 978-1-61779-111-6
  4. 2017, ATM Kinase: Methods and Protocols, Methods in Molecular Biology, vol. 1599,113-126,2017, Zn(II)–Phos-tag SDS-PAGE for Separation and Detection of a DNA Damage-Related Signaling Large Phosphoprotein, 2017, Scholarly Book, Joint work, 英語, Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike
  5. 2016, Methods and Protocols, Methods in Molecular Biology,, Phosphopeptide detection with biotin-labeled Phos-tag., Springer, New York, 2016, Scholarly Book, Joint work, 英語, Kinoshita-Kikuta, E., Eiji Kinoshita, and Koike, T.

Invited Lecture, Oral Presentation, Poster Presentation

  1. Protein-N-myristoylation-dependent Phosphorylation of Serine 13 of Tyrosine Kinase Lyn by Casein Kinase 1g at the Golgi during the Intracellular Protein Traffic, Without Invitation, Japanese
  2. Quantitative monitoring of His and Asp phosphorylation in a bacterial signaling system by using Phos-tag Magenta/Cyan fluorescent dyes, 2020/09/14, Without Invitation, Japanese
  3. Quantitative analysis of phosphoprotein blotted on PVDF membrane using fluorescent Phos-tag derivatives, 2020/09/14, Without Invitation, Japanese
  4. TAMRA-Phostagを用いたリン酸化タンパク質のゲル染色法, 草本寛，西村朋世，木下恵美子，木下英司，小池透, 第91回日本生化学会大会, 2018/09/25, Without Invitation, Japanese
  5. TAMRA-Phostagを用いたリン酸化タンパク質のゲル染色法　, 草本寛，西村朋世，木下恵美子，木下英司，小池透, 第69回日本電気泳動学会, 2018/08/07, Without Invitation, Japanese
  6. N-ミリストイル化依存的なタンパク質リン酸化反応のPhos-tag SDS-PAGEを用いた解析法の確立, 谷川 綾音、木下 恵美子、黄波戸 亜哉、守屋 康子、木下 英司、小池 透、内海 俊彦, 第５９回日本生化学会　中国四国支部例会, 2018/05/10, Without Invitation, Japanese
  7. Phos-tag SDS-PAGEを用いたN-ミリストイル化依存的なタンパク質リン酸化反応の解析, 谷川 綾音、木下 恵美子、黄波戸 亜哉、守屋 康子、木下 英司、小池 透、内海 俊彦, 日本農芸化学会大会, 2018/03/01, Without Invitation, Japanese
  8. 亜鉛酵素モデルに対するリガンドの親和性を解析する新規分光分析法の開発, 草本 寛，芝 晃生，常弘 昌弥，藤岡 晴人，木下 恵美子，木下 英司，小池 透, 日本薬学会第138年会, 2018/03/28, Without Invitation, Japanese
  9. 蛍光クエンチングを用いるアルカリホスファターゼ分析の新規蛍光分析法の開発, 芝 晃生，木下 恵美子，木下　英司，小池 透, 日本薬学会第138年会, 2018/03/28, Without Invitation, Japanese
  10. プロテインキナーゼのリン酸化動態のプロファイリング法の開発, 上里裕樹、木下英司、木下恵美子、小池透、亀下勇、杉山康憲, 2017年度生命科学系学会合同年会 ComBio2017, 2017/12/08, Without Invitation, Japanese
  11. Phos-tagを基盤としたin vitro キナーゼアッセイ法：宿主細胞に由来する偽陽性のキナーゼ活性を見極める有効手段, 木下英司，木下恵美子，小池 透, 2017年度生命科学系学会合同年次大会（ConBio2017）, 2017/12/08, Without Invitation, Japanese
  12. Phos-tag SDS-PAGEを基盤としたin vitro キナーゼアッセイ法： 宿主細胞に由来する偽陽性のキナーゼ活性を見極める, 藤田萌々子・木下恵美子・木下英司・小池透, 第６８回日本電気泳動学会, 2017/11/24, Without Invitation, Japanese
  13. ハイブリッドヒスチジンキナーゼにおけるリン酸基転移反応様式の検証, 池上まどか・木下恵美子・木下英司・小池透, 第６８回日本電気泳動学会, 2017/11/24, Without Invitation, Japanese
  14. 細胞内情報伝達におけるMEK1リン酸化種の動態解析, 谷口翼・上田爽夏・石山美帆・木下恵美子・木下英司・小池透, 第６８回日本電気泳動学会, 2017/11/24, Without Invitation, Japanese
  15. Phos-tag SDS-PAGEの移動度に影響を及ぼすリン酸基以外の要因, 木下恵美子・山戸護久・西山敦洋・河野敏己・木下英司・小池透, 第６８回日本電気泳動学会, 2017/11/24, Without Invitation, Japanese
  16. マルチPK抗体とPhos-tag 2D-PAGEを組み合わせたプロテインキナーゼのプロファイル法, 上里裕樹, 木下英司, 木下恵美子, 小池透,亀下勇, 杉山康憲, 第６８回日本電気泳動学会, 2017/11/24, Without Invitation, Japanese
  17. タンパク質のリン酸化分子種全体を見える化するPhos-tag SDS-PAGE, 木下恵美子, 日本プロテオーム学会2017年大会, 2017/07/28, Without Invitation, Japanese
  18. フォスタグ技術とプロテオミクス情報を活用した細胞内MEK1リン酸化ダイナミクスのプロファイリング, 木下英司，木下恵美子，小池透, 第137回日本薬学会年会, 2017/03/24, Without Invitation, Japanese
  19. Phosphoprotein Gel Stain by Fluorophore-labeled Phos-tag Derivatives, 2019/07/25, Without Invitation, Japanese
  20. Protein Phosphorylation Analysis Using Phos-tag-based Techniques, 2019/07/25, Without Invitation, Japanese

Patented

  1. Patent
  2. Patent, リン酸基を有する物質の染色方法

External Funds

Acceptance Results of Competitive Funds

  1. 科学研究費補助金　基盤研究（C）, タンパク質のリン酸化状態から疾患の病態をプロファイリングするシステムの開発, 2018, 2020
  2. KAKENHI, 2015, 2017
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  3. 2014年度　薬学系研究奨励, チオリン酸化反応を利用した脆弱なタンパク質リン酸基転移反応中間体の追跡, 2014, 2015
  4. 研究成果展開事業 研究成果最適展開支援プログラム(A-STEP), 亜鉛フォスタグ電気泳動用中性プレキャストゲルの開発, 2012, 2013
  5. KAKENHI, 2012, 2014
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  6. Ｈ２２年度産学官連携新産業創出研究会, 生体内リン酸蛋白質を分離精製するためのカラムクロマトデバイスの開発・商品化, 2010, 2011
  7. KAKENHI, A method for simultaneous detection of dual post-translational modifications using phosphate-affinity electrophoresis, 2010, 2011
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  8. KAKENHI, Development of phosphate-affinity two-dimensional fluorescence difference gel electrophoresis, 2008, 2009
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  9. リン酸アフィニティーマイクロチップ電気泳動法の開発 ～セルロース繊維を利用した生体内リン酸化分子認識ゲル媒体の構築～, 2007, 2008
  10. 平成19年度 シーズ発掘試験, リン酸基結合ナノ分子を用いた高感度メチル化DNA検出法の開発, 2007, 2008
  11. 平成18年度 シーズ発掘試験, 高流速対応型の新規リン酸アフィニティークロマトグラフィーカラムの開発, 2006, 2007
  12. KAKENHI, 2006, 2007
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Social Activities

Organizing Academic Conferences, etc.

  1. 日本電気泳動学会　第68回総会, 2017/11