Last Updated :2024/02/01

Affiliations, Positions
Hiroshima University Hospital(Center for Oral Clinical Examination), Professor
Periodontitis is an inflammatory periodontal tissue-destructive disease attributed to host immune responses to periodontal pathogenic bacteria, which leads to tooth loss. Otherwise, tissue regenerative therapy using stem cells, which exerts self-renewing properties and multipotency, has attracted scientific and medical attention. Thus, using mesenchymal stem cells and our own developed three-dimensional culture system, we will develop the novel promising periodontal tissue regenerative cell therapy.

Basic Information

Academic Degrees

  • Doctor of Philosophy in Dental Science, Hiroshima University

Educational Activity

  • [Bachelor Degree Program] School of Dentistry : Program of Dentistry : Dentistry
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Biomedical Sciences : Program of Dental Sciences

Research Fields

  • Medicine,dentistry, and pharmacy;Dentistry;Periodontology

Educational Activity

Course in Charge

  1. 2023, Undergraduate Education, 2Term, Topics in Dental Research II
  2. 2023, Undergraduate Education, 3Term, Examination and Diagnosis
  3. 2023, Undergraduate Education, First Semester, Dental Research Practices I
  4. 2023, Undergraduate Education, Second Semester, Dental Research Practices II
  5. 2023, Undergraduate Education, Academic Year, Clinical Practice (Oral Clinical Examination)
  6. 2023, Undergraduate Education, 1Term, Oral Health Management A
  7. 2023, Undergraduate Education, First Semester, Overview of Oral Engineering
  8. 2023, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Innovation & Precision Dentistry
  9. 2023, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Innovation & Precision Dentistry
  10. 2023, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Innovation & Precision Dentistry
  11. 2023, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Innovation & Precision Dentistry

Research Activities

Academic Papers

  1. Effect of neurotrophins on differentiation, calcification and proliferation in cultures of human pulp cells, Cell Biol Int, 1462-1469, 20071231
  2. Irsogladine maleate abolishes the increase in interleukin-8 levels caused by outer membrane protein 29 from Aggregatibacter (Actinobacillus) actinomycetemcomitans through the ERK pathway in human gingival epithelial cells., J Periodontal Res, 43(5), 508-513, 200810
  3. Effect of neurotrophin-4/5 on bone/cementum-related protein expressions and DNA synthesis in cultures of human periodontal ligament cells., J Periodontol, 79(11), 2182-2189, 200811
  4. Humoral factors released from human periodontal ligament cells influence calcification and proliferation in human bone marrow mesenchymal stem cells., Journal of Periodontology, 79(12), 2361-2370, 200812
  5. Neodymium-doped yttrium-aluminium-garnet laser irradiation abolishes the increase in interleukin-6 levels caused by peptidoglycan through the p38 mitogen-activated protein kinase pathway in human pulp cells, Journal of Endodontics, 35(3), 373-376, 200903
  6. Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate, Biochemical and Biophysical Research Communications, 386(1), 11-15, 20090814
  7. Hydrogen from intestinal bacteria is protective for Concanavalin A-induced hepatitis, Biochemical and Biophysical Research Communications, 386(2), 316-321, 20090821
  8. Brain-derived neurotrophic factor protects cementoblasts from serum starvation-induced cell death, Journal of Cellular Physiology, 221(3), 696-706, 200912
  9. Bacteria-derived hydrogen sulfide promotes IL-8 production from epithelial cells, Biochemical and Biophysical Research Communications, 391(1), 645-650, 20100101
  10. Role of periodontal pathogenic bacteria in RANKL-mediated bone destruction in periodontal disease, Journal of Oral Microbiology, 8(2), 201011
  11. Irsogladine maleate regulates neutrophil migration and E-cadherin expression in gingival epithelium stimulated by Aggregatibacter actinomycetemcomitans, Biochemical Pharmacology, 79(10), 1496-1505, 20100515
  12. The antimicrobial peptide LL37 induces the migration of human pulp cells: a possible adjunct for regenerative endodontics, Journal of Endodontics, 36(6), 1009-1013, 201006
  13. Characteristics of high-molecular-weight hyaluronic acid as a brain-derived neurotrophic factor scaffold in periodontal tissue regeneration, Tissue Engineering PartA, 17(7), 955-967, 201104
  14. Additive effects of orexin B and vasoactive intestinal polypeptide on LL-37-mediated antimicrobial activities, Journal of Neuroimmunology, 233(1), 37-45, 201104
  15. Inhibition of matrix metalloproteinase-9 activity by doxycycline ameliorates RANK ligand-induced osteoclast differentiation in vitro and in vivo, Experimental Cell Research, 317(10), 1454-1464, 20110610
  16. Aggregatibacter actinomycetemcomitans Omp29 is associated with bacterial entry to gingival epithelial cells by F-actin rearrangement, PLoS ONE, 6(4), e18287, 201104
  17. Irsogladine maleate counters the interleukin-1 beta-induced suppression in gap-junctional intercellular communication but does not affect the interleukin-1 beta-induced zonula occludens protein-1 levels in human gingival epithelial cells, J Periodontal Res, 43(1), 96-102, 200802
  18. Brain-derived neurotrophic factor stimulates bone/cementum-related protein gene expression in cementoblasts, J Biol Chem, 20080606
  19. Selective serotonin reuptake inhibitors attenuate the antigen presentation from dendritic cells to effector T lymphocytes, FEMS Immunology & Medical Microbiology, 62(3), 283-294, 201108
  20. Expression and possible immune-regulatory function of ghrelin in oral epithelium, Journal of Dental Research, 90(11), 1286-1292, 201111
  21. A role of oral bacteria in bisphosphonate-induced osteonecrosis of the jaw, Journal of Dental Research, 90(11), 1339-1345, 201111
  22. Blocking of sodium and potassium ion-dependent adenosine triphosphatase-α1 with ouabain and vanadate suppresses cell-cell fusion during RANKL-mediated osteoclastogenesis, European Journal of Pharmacology, 30(670), 409-418, 20111130
  23. Muscarinic type 3 receptor induces cytoprotective signaling in salivary gland cells through epidermal growth factor receptor transactivation, Molecular Pharmacology, 82(1), 115-24, 20120701
  24. The antimicrobial activity of the appetite peptide hormone ghrelin, Peptides, 36(2), 151-6, 20120801
  25. Brain-derived neurotrophic factor induces migration of endothelial cells through a TrkB-ERK-integrin alpha(V)beta(3)-FAK cascade, JOURNAL OF CELLULAR PHYSIOLOGY, 227(5), 2123-2129, 201203
  26. Detection of periodontal pathogens in newborns and children with mixed dentition, 検索結果 European Journal of Clinical Microbiology & Infectious Diseases, 31(6), 1041-1050, 201206
  27. Fluoxetine inhibits inflammatory response and bone loss in a rat model of ligature-induced periodontitis., Journal of Periodontology, 83(5), 664-671, 201205
  28. Bacteria-reactive immune response may induce RANKL-expressing T cells in the mouse periapical bone loss lesion, Journal of Endodontics, 38(3), 346-350, 201203
  29. Expression levels of novel cytokine IL-32 in periodontitis and its role in the suppression of IL-8 production by human gingival fibroblasts stimulated with Porphyromonas gingivalis, Journal of Oral Microbiology, 4, 14832, 20120516
  30. GlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence, PLoS ONE, 7(3), e33382, 20120516
  31. Irsogladine maleate regulates the inflammatory related genes in human gingival epithelial cells stimulated by Aggregatibacter actinomycetemcomitans, INTERNATIONAL IMMUNOPHARMACOLOGY, 15(2), 340-347, 201302
  32. Comprehensive Oral Management for Collaborative Medical and Dental Care -Practical Approaches for Oral Care Support Team-, The Journal of Hiroshima University Dental Society, 45(1), 1-6, 20130601
  33. Antimicrobial peptide LL37 promotes vascular endothelial growth factor-A expression in human periodontal ligament cells, JOURNAL OF PERIODONTAL RESEARCH, 48(2), 228-234, 201304
  34. The antimicrobial peptide LL37 promotes bone regeneration in a rat calvarial bone defect, PEPTIDES, 46, 136-142, 201308
  35. The Keap1/Nrf2 Protein Axis Plays a Role in Osteoclast Differentiation by Regulating Intracellular Reactive Oxygen Species Signaling, JOURNAL OF BIOLOGICAL CHEMISTRY, 288(32), 23009-23020, 20130809
  36. BDNF mimetic compound LM22A-4 regulates cementoblast differentiation via the TrkB-ERK/Akt signaling cascade, INTERNATIONAL IMMUNOPHARMACOLOGY, 19(2), 245-252, 201409
  37. miR-584 Expressed in Human Gingival Epithelial Cells Is Induced by Porphyromonas gingivalis Stimulation and Regulates Interleukin-8 Production via Lactoferrin Receptor, JOURNAL OF PERIODONTOLOGY, 85(6), E198-E204, 201406
  38. Amphotericin B down-regulates Aggregatibacter actinomycetemcomitans-induced production of IL-8 and IL-6 in human gingival epithelial cells, CELLULAR IMMUNOLOGY, 290(2), 201-208, 201408
  39. Smad2 is Involved in Aggregatibacter actinomycetemcomitans-induced Apoptosis, JOURNAL OF DENTAL RESEARCH, 93(11), 1148-1154, 201411
  40. Nuclear Nrf2 Induction by Protein Transduction Attenuates Osteoclastogenesis, FREE RADICAL BIOLOGY AND MEDICINE, 77, 239-248, 201412
  41. Houttuynia cordata suppresses the Aggregatibacter actinomycetemcomitans-induced increase of inflammatory-related genes in cultured human gingival epithelial cells, JOURNAL OF DENTAL SCIENCES, 10(1), 88-94, 201503
  42. The differential expression of mgl mRNA by Porphyromonas gingivalis affects the production of methyl mercaptan, ORAL DISEASES, 21(5), 626-633, 201507
  43. Clumps of a mesenchymal stromal cell/extracellular matrix complex can be a novel tissue engineering therapy for bone regeneration, CYTOTHERAPY, 17(7), 860-873, 201507
  44. Brain-derived neurotrophic factor prevents the endothelial barrier dysfunction induced by interleukin-1 beta and tumor necrosis factor-alpha, JOURNAL OF PERIODONTAL RESEARCH, 50(4), 444-451, 201508
  45. Interleukin-8 induces DNA synthesis, migration and down-regulation of cleaved caspase-3 in cultured human gingival epithelial cells, JOURNAL OF PERIODONTAL RESEARCH, 50(4), 479-485, 201508
  46. Irsogladine maleate inhibits Porphyromonas gingivalis-mediated expression of toll-like receptor 2 and interleukin-8 in human gingival epithelial cells, JOURNAL OF PERIODONTAL RESEARCH, 50(4), 486-493, 201508
  47. LL37 induces VEGF expression in dental pulp cells through ERK signalling, INTERNATIONAL ENDODONTIC JOURNAL, 48(7), 673-679, 201507
  48. Involvement of smad2 and Erk/Akt cascade in TGF-beta 1-induced apoptosis in human gingival epithelial cells, CYTOKINE, 75(1), 165-173, 201509
  49. Sequential process in brain-derived neurotrophic factor-induced functional periodontal tissue regeneration, EUROPEAN JOURNAL OF ORAL SCIENCES, 124(2), 141-150, 201604
  50. Distinction Between Cell Proliferation and Apoptosis Signals Regulated by Brain-Derived Neurotrophic Factor in Human Periodontal Ligament Cells and Gingival Epithelial Cells, JOURNAL OF CELLULAR BIOCHEMISTRY, 117(7), 1543-1555, 201607
  51. Brain-Derived Neurotrophic Factor Inhibits Intercellular Adhesion Molecule-1 Expression in Interleukin-1 beta-Treated Endothelial Cells, CELL BIOCHEMISTRY AND BIOPHYSICS, 74(3), 399-406, 201609
  52. Azithromycin recovers reductions in barrier function in human gingival epithelial cells stimulated with tumor necrosis factor-alpha, ARCHIVES OF ORAL BIOLOGY, 62, 64-69, 201602
  53. Porphyromonas gingivalis infection exacerbates the onset of rheumatoid arthritis in SKG mice, CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 186(2), 177-189, 201611
  54. A novel method of sampling gingival crevicular fluid from a mouse model of periodontitis, JOURNAL OF IMMUNOLOGICAL METHODS, 438, 21-25, 201611
  55. Aggregatibacter actinomycetemcomitans outer membrane protein 29 (Omp29) induces TGF--regulated apoptosis signal in human gingival epithelial cells via fibronectin/integrin1/FAK cascade, CELLULAR MICROBIOLOGY, 18(12), 1723-1738, 201612
  56. Mobilization of TLR4 Into Lipid Rafts by Aggregatibacter Actinomycetemcomitans in Gingival Epithelial Cells, CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 39(5), 1777-1786, 2016
  57. Brain-Derived Neurotrophic Factor Inhibits Peptidoglycan-Induced Inflammatory Cytokine Expression in Human Dental Pulp Cells, INFLAMMATION, 40(1), 240-247, 201702
  58. Xenotransplantation of interferon-gamma-pretreated clumps of a human mesenchymal stem cell/extracellular matrix complex induces mouse calvarial bone regeneration, STEM CELL RESEARCH & THERAPY, 8, 20170426
  59. A novel gingival overgrowth mouse model induced by the combination of CsA and ligature-induced inflammation, JOURNAL OF IMMUNOLOGICAL METHODS, 445, 31-36, 201706
  60. DC-STAMP Is an Osteoclast Fusogen Engaged in Periodontal Bone Resorption, JOURNAL OF DENTAL RESEARCH, 96(6), 685-693, 201706
  61. MSC/ECM Cellular Complexes Induce Periodontal Tissue Regeneration, JOURNAL OF DENTAL RESEARCH, 96(9), 984-991, 201708
  62. Effect of local bone marrow stromal cell administration on ligature-induced periodontitis in mice, JOURNAL OF ORAL SCIENCE, 59(4), 629-637, 201712
  63. Cryopreserved clumps of mesenchymal stem cell/extracellular matrix complexes retain osteogenic capacity and induce bone regeneration, STEM CELL RESEARCH & THERAPY, 9, 20180321
  64. Regulation of defensive function on gingival epithelial cells can prevent periodontal disease, JAPANESE DENTAL SCIENCE REVIEW, 54(2), 66-75, 201803
  65. The induced RNA-binding protein, HuR, targets 3-UTR region of IL-6 mRNA and enhances its stabilization in periodontitis, CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 192(3), 325-336, 201806
  66. Reparative bone-like tissue formation in the tooth of a systemic sclerosis patient, INTERNATIONAL ENDODONTIC JOURNAL, 51(9), 1059-1066, 201809
  67. Intravital endoscopic technology for real-time monitoring of inflammation caused in experimental periodontitis., J Immunol Methods, 457, 26-29, 201806
  68. The involvement of C5a in the progression of experimental arthritis with Porphyromonas gingivalis infection in SKG mice, ARTHRITIS RESEARCH & THERAPY, 20, 20181103
  69. Type I collagen deposition via osteoinduction ameliorates YAP/TAZ activity in 3D floating culture clumps of mesenchymal stem cell/extracellular matrix complexes, STEM CELL RESEARCH & THERAPY, 9, 20181207
  70. Intravital endoscopic technology for real-time monitoring of inflammation caused in experimental periodontitis, JOURNAL OF IMMUNOLOGICAL METHODS, 457, 26-29, 201806
  71. BDNF/HMW-HA complex as an adjunct to nonsurgical periodontal treatment of ligature-induced periodontitis in dogs, JOURNAL OF PERIODONTOLOGY, 90(1), 98-109, 201901
  72. Glycyrrhizic acid suppresses inflammation and reduces the increased glucose levels induced by the combination of Porphyromonas gulae and ligature placement in diabetic model mice, INTERNATIONAL IMMUNOPHARMACOLOGY, 68, 30-38, 201903
  73. Clumps of Mesenchymal Stem Cell/Extracellular Matrix Complexes Generated with Xeno-Free Conditions Facilitate Bone Regeneration via Direct and Indirect Osteogenesis, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(16), 20190802
  74. Relationship between periodontal inflammation and calcium channel blockers induced gingival overgrowth-a cross-sectional study in a Japanese population, CLINICAL ORAL INVESTIGATIONS, 23(11), 4099-4105, 201911
  75. Multiple External Root Resorption of Teeth as a New Manifestation of Systemic Sclerosis-A Cross-Sectional Study in Japan, JOURNAL OF CLINICAL MEDICINE, 8(10), 201910
  76. Microbe-Dependent Exacerbated Alveolar Bone Destruction in Heterozygous Cherubism Mice, JBMR PLUS, 4(6), 202006
  77. Alveolar Bone Protection by Targeting the SH3BP2-SYK Axis in Osteoclasts, JOURNAL OF BONE AND MINERAL RESEARCH, 35(2), 382-395, 202002
  78. Periodontal ligament cells regulate osteogenesis via miR-299-5p in mesenchymal stem cells, DIFFERENTIATION, 112, 47-57, 202004
  79. Optineurin regulates osteoblastogenesis through STAT1, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 525(4), 889-894, 20200514
  80. Regulation of osteogenesis via miR-101-3p in mesenchymal stem cells by human gingival fibroblasts, JOURNAL OF BONE AND MINERAL METABOLISM, 38(4), 442-455, 202007
  81. Aggressive periodontitis and NOD2 variants, JOURNAL OF HUMAN GENETICS, 65(10), 841-846, 202010
  82. Cox2-mediated PGE2 production via p38/JNK-c-fos signaling inhibits cell apoptosis in 3D floating culture clumps of mesenchymal stem cell/extracellular matrix complexes, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 530(2), 448-454, 20200917
  83. IL-6 Induced by Periodontal Inflammation Causes Neuroinflammation and Disrupts the Blood-Brain Barrier, BRAIN SCIENCES, 10(10), 202010
  84. Effect ofPorphyromonas gingivalisinfection on gut dysbiosis and resultant arthritis exacerbation in mouse model, ARTHRITIS RESEARCH & THERAPY, 22(1), 20201019
  85. Identification of regulatory mRNA and microRNA for differentiation into cementoblasts and periodontal ligament cells, JOURNAL OF PERIODONTAL RESEARCH, 56(1), 69-82, 202101
  86. Functional Regulatory Mechanisms Underlying Bone Marrow Mesenchymal Stem Cell Senescence During Cell Passages, CELL BIOCHEMISTRY AND BIOPHYSICS, 79(2), 321-336, 202106
  87. 3D Clumps/Extracellular Matrix Complexes of Periodontal Ligament Stem Cells Ameliorate the Attenuating Effects of LPS on Proliferation and Osteogenic Potential, JOURNAL OF PERSONALIZED MEDICINE, 11(6), 202106
  88. The role of nuclear receptor 4A1 (NR4A1) in drug-induced gingival overgrowth, FASEB JOURNAL, 35(7), 202107
  89. Involvement of Rac1 in macrophage activation by brain-derived neurotrophic factor, MOLECULAR BIOLOGY REPORTS, 48(6), 5249-5257, 202106
  90. External cervical resorption detected via cone-beam computed tomography in a patient with myelin oligodendrocyte glycoprotein antibody-associated disease: A case report, CLINICAL CASE REPORTS, 9(7), 202107
  91. Clumps of Mesenchymal Stem Cells/Extracellular Matrix Complexes Generated with Xeno-Free Chondro-Inductive Medium Induce Bone Regeneration via Endochondral Ossification, BIOMEDICINES, 9(10), 202110
  92. Editorial: Stem Cells in Oral Cavity: From Development to Regeneration, Frontiers in Cell and Developmental Biology, 10, 840771-840771, 20220120
  93. Periapical lesion following Cnm-positive Streptococcus mutans pulp infection worsens cerebral hemorrhage onset in an SHRSP rat model, CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 210(3), 321-330, 20221231
  94. Anti-Inflammatory Effects of Geniposidic Acid on Porphyromonas gingivalis-Induced Periodontitis in Mice, BIOMEDICINES, 10(12), 202212
  95. Integrating Genetic Alterations and the Hippo Pathway in Head and Neck Squamous Cell Carcinoma for Future Precision Medicine, JOURNAL OF PERSONALIZED MEDICINE, 12(10), 202210
  96. Induction of functional xeno-free MSCs from human iPSCs via a neural crest cell lineage, NPJ REGENERATIVE MEDICINE, 7(1), 20220915
  97. Clumps of mesenchymal stem cells/extracellular matrix complexes directly reconstruct the functional periodontal tissue in a rat periodontal defect model, JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 16(10), 945-955, 202210
  98. Masticatory dysfunction in patients with diabetic neuropathy: A cross-sectional study, PLOS ONE, 17(6), 2022
  99. Xenotransplantation of cryopreserved human clumps of mesenchymal stem cells/extracellular matrix complexes pretreated with IFN-? induces rat calvarial bone regeneration, REGENERATIVE THERAPY, 20, 117-125, 202206
  100. Cancellous bone-like tissue replacement from calcinosis in patients with systemic sclerosis with multiple external root resorption, BONE REPORTS, 16, 202206
  101. Microbial differences between active and remission peri-implantitis, SCIENTIFIC REPORTS, 12(1), 20220328
  102. Cytokines regulate stemness of mesenchymal stem cells via miR-628-5p during periodontal regeneration, JOURNAL OF PERIODONTOLOGY, 93(2), 269-286, 202202


  1. Patent, 8992998, 2015/03/31, MAPK inhibition by H2

External Funds

Acceptance Results of Competitive Funds

  1. KAKENHI, 2017, 2018
  2. KAKENHI, 2014, 2015
  3. KAKENHI, Development of novel periodontal tissue regenerative therapy by small molecule ligands induced TrkB transactivation, 2012, 2013