HISAKAGE FUNABASHI

Last Updated :2022/08/02

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Associate Professor
Web Site
Self-introduction
Our research focuses on the utilization of biomolecules and living cells as functional materials. We are developing novel functional molecules such as biosensing molecules with proteins or nucleic acids. We are also exploring new methods to create, evaluate, and manipulate functional living cells.

Basic Information

Academic Degrees

  • Doctor of Engineering, Tokyo Institute of Technology
  • Master of Engineering, Tokyo Institute of Technology

Educational Activity

  • [Bachelor Degree Program] School of Engineering : Cluster 3(Applied Chemistry, Biotechnology and Chemical Engineering) : Program of Biotechnology
  • [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biotechnology
  • [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biotechnology

Research Fields

  • Engineering;Process / Chemical engineering;Biofunction / Bioprocess

Affiliated Academic Societies

  • The Electrochemical Society of Japan
  • The Chemical Society of Japan
  • The Society for Biotechnology, Japan
  • American Chemical Society

Educational Activity

Course in Charge

  1. 2022, Liberal Arts Education Program1, 2Term, Introduction to Applied Chemistry, Chemical Engineering,and Biotechnology
  2. 2022, Liberal Arts Education Program1, 1Term, Introductory Seminar for First-Year Students
  3. 2022, Undergraduate Education, 2Term, Biochemistry I
  4. 2022, Undergraduate Education, Second Semester, Experiments on Biotechnology II
  5. 2022, Graduate Education (Master's Program) , 4Term, Multifunctional Sensing Techniques
  6. 2022, Graduate Education (Master's Program) , Academic Year, Seminar in Biotechnology
  7. 2022, Graduate Education (Master's Program) , 1Term, Exercises in Biotechnology A
  8. 2022, Graduate Education (Master's Program) , 2Term, Exercises in Biotechnology A
  9. 2022, Graduate Education (Master's Program) , 3Term, Exercises in Biotechnology B
  10. 2022, Graduate Education (Master's Program) , 4Term, Exercises in Biotechnology B
  11. 2022, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biotechnology
  12. 2022, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences

Research Activities

Academic Papers

  1. Biosensors Using Engineered Protein, Encyclopedia of Sensors and Biosensors, 1st Edition, 2022
  2. Design of Split G-quadruplex-based DNA–Bridged Nucleic Acid Chimera Nanotweezers that Recognize Short Nucleic Acids with a Single-base Mismatch, Sens. Mater., 2022
  3. A BRET-Based Homogeneous Insulin Assay Using Interacting Domains in the Primary Binding Site of the Insulin Receptor, Analytical Chemistry, 87(5), 2764-2770, 20150303
  4. A FRET-based DNA nano-tweezer technique for the imaging analysis of specific mRNA., Analyst, 140(4), 999-1003, 2015
  5. A split G-quadruplex-based DNA nano-tweezers structure as a signal-transducing molecule for the homogeneous detection of specific nucleic acids, Biosensors & Bioelectronics, 74, 222-226, 20151215
  6. Porphyrin-uptake in liposomes and living cells using an exchange method with cyclodextrin, RSC Advances, 5(127), 105279-105287, 2015
  7. A mechanical metamaterial made from a DNA hydrogel, Nature Nanotechnology, 7(12), 816-820, 2012
  8. Utilization of Fluorescent Glucose Analog 2-NBDG as a Metabolic Indicator for FACS Analysis during ES Cell Differentiation, Electrochemistry, 80(5), 299-301, 20120505
  9. Non-destructive monitoring of rpoS promoter activity as stress marker for evaluating cellular physiological status., Journal of Biotechnology, 95(1), 85-93, 2002
  10. Intracellular delivery of antibodies using TAT fusion protein A., Biochemical and Biophysical Research Communications, 310(3), 730-734, 2003
  11. On-chip biosensing of estrogen receptor-alpha at single molecular level., Biosensors & Bioelectronics, 19(12), 1573-1579, 2004
  12. Construction of streptavidin-luciferase fusion protein for ATP sensing with fixed form., Biotechnology Letters, 26(13), 1061-1066, 2004
  13. Electrochemical evaluation of cellular physiological status under stress in Escherichia coli with the rpoS-lacZ reporter gene., Biotechnology and Bioengineering, 90(4), 509-515, 2005
  14. Method for detection of specific nucleic acids by recombinant protein with fluorescent resonance energy transfer., Analytical Chemistry, 77(14), 4308-4314, 2005
  15. Activity-based in vitro selection of T4 DNA ligase., Biochemical and Biophysical Research Communications, 336(3), 987-993, 2005
  16. Glucose oxidase assisted homogeneous electrochemical receptor binding assay for drug screening., Biosensors & Bioelectronics, 21(9), 1675-1683, 2006
  17. Fabrication of an antibody microwell array with self-adhering antibody binding protein., Analytical Biochemistry, 350(2), 298-303, 2006
  18. Construction of epidermal growth factor fusion protein with cell adhesive activity., Biomaterials, 27(18), 3451-3458, 2006
  19. Cell-surface-localized ATP detection with immobilized firefly luciferase., Analytical Biochemistry, 352(1), 61-67, 2006
  20. In vitro selection of zinc finger DNA-binding proteins through ribosome display., Biochemical and Biophysical Research Communications, 345(3), 1149-1154, 2006
  21. Transduction of NeuroD2 protein induced neural cell differentiation., Journal of Biotechnology, 126(2), 230-236, 2006
  22. Delivery of antibody-captured proteins into living cells using PTD-fused protein A., Biotechnology Letters, 28(15), 1209-1214, 2006
  23. Assessment of small ligand-protein interactions by electrophoretic mobility shift assay using DNA-modified ligand as a sensing probe., Biotechnology Letters, 29(5), 785-789, 2007
  24. Design of a thermocontrollable protein complex., Bioconjugate Chemistry, 18(5), 1619-1624, 2007
  25. Pore-scale quantification of colloid transport in saturated porous media., Environmental Science & Technology, 42(2), 517-523, 2008
  26. A cell-free protein-producing gel., Nature Materials, 8(5), 432-437, 2009
  27. Multifunctional nanoarchitectures from DNA-based ABC monomers., Nature Nanotechnology, 4(7), 430-436, 2009
  28. Evaluation of small ligand-protein interaction by ligation reaction with DNA-modified ligand., Biotechnology Letters, 32(1), 97-102, 2010
  29. High-yield cell-free protein production from P-gel., Nature Protocols, 4(12), 1759-1770, 2009
  30. Sox2 regulatory region 2 sequence works as a DNA nuclear targeting sequence enhancing the efficiency of an exogenous gene expression in ES cells., Biochemical and Biophysical Research Communications, 400(4), 554-558, 2010
  31. A novel glucosylation reaction on anthocyanins catalyzed by acyl-glucose-dependent glucosyltransferase in the petals of carnation and delphinium., The Plant Cell, 22(10), 3374-3389, 2010
  32. A femto-injection technique for dynamic analysis of protein function in living embryonic stem cells., Biotechnology Letters, 34(7), 1257-1262, 2012
  33. Tryptic soy medium is feasible for the in situ preparation of standards containing small defined numbers of microbial cells., Journal of Microbiological Methods, 93(1), 49-51, 2013
  34. Targeted delivery of a decoy oligodeoxynucleotide to a single ES cell by femtoinjection., Nanomedicine : Nanotechnology, Biology, and Medicine, 9(7), 855-863, 2013
  35. Analysis of odor compounds in feces of mice that were exposed to various stresses during breeding., Experimental Animals / Japanese Association for Laboratory Animal Science, 62(2), 101-107, 2013
  36. Development of a protocol for selection of genes fit for the in vivo knockdown method and its application to insulin receptor substrate genes in mice., Experimental Animals / Japanese Association for Laboratory Animal Science, 62(2), 117-125, 2013
  37. Potential use of Lactobacillus cell density in feces as a non-invasive bio-indicator for evaluating environmental stress during mouse breeding., Biocontrol Science, 18(2), 101-104, 2013
  38. A universal DNA-based protein detection system., Journal of the American Chemical Society, 135(38), 14008-14011, 2013
  39. Assembly of zinc finger motif-fused enzymes on a dsDNA scaffold for catalyzing consecutive reactions with a proximity effect., Biotechnology Letters, 37(1), 109-114, 2015
  40. Hemin/G-quadruplex Complex as a Signal Generator for Electrochemical Assays of Bioanalytes, Electrochemistry, 84(5), 290-295, 201605
  41. Continuous Monitoring of Specific mRNA Expression Responses with a Fluorescence Resonance Energy Transfer-Based DNA Nano-tweezer Technique That Does Not Require Gene Recombination, Analytical Chemistry, 88(16), 7894-7898, 20160816
  42. Noise-free accurate count of microbial colonies by time-lapse shadow image analysis, Journal of Microbiological Methods, 91(3), 420-428, 2012
  43. Biodegradable CpG DNA hydrogels for sustained delivery of doxorubicin and immunostimulatory signals in tumor-bearing mice, Biomaterials, 32(2), 488-494, 2011
  44. The assembly of a short linear natural cytosine-phosphate-guanine DNA into dendritic structures and its effect on immunostimulatory activity, Biomaterials, 30(29), 5701-5706, 2009
  45. Construction of intramolecular luciferase complementation probe for detecting specific RNA, Bioconjugate Chemistry, 18(3), 956-962, 2007
  46. Bioluminescent enumeration of surface antigen-specific cells using the streptavidin-luciferase fusion protein, Sensors and Actuators B: Chemical, 120(1), 51-56, 2006
  47. Fluorescent Monitoring of Cellular Physiological Status Depending on The Accumulation of ppGpp, Biotechnology Letters, 24(4), 269-273, 2002
  48. Bioluminescent Monitoring of Intracellular ATP during Fermentation, Luminescence, 14(6), 291-296, 1999
  49. A Novel Biocontainment Strategy Makes Bacterial Growth and Survival Dependent on Phosphite, Scientific Reports, 7, 44748, 2017
  50. Improved photodynamic activities of liposome-incorporated [60]fullerene derivatives bearing a polar group, Chemical Communications, 53(20), 2966-2969, 2017
  51. Photodynamic Activities of Porphyrin Derivative-Cyclodextrin Complexes by Photoirradiation, ACS Medicinal Chemistry Letters, 8(5), 555-559, 2017
  52. Improvement of Photodynamic Activity of Lipid-Membrane-Incorporated Fullerene Derivative by Combination with a Photo-Antenna Molecule, CHEMISTRY-A EUROPEAN JOURNAL, 24(29), 7335-7339, 20180523
  53. Synthetic Phosphorus Metabolic Pathway for Biosafety and Contamination Management of Cyanobacterial Cultivation, ACS SYNTHETIC BIOLOGY, 7(9), 2189-2198, 201809
  54. Stabilisation of lipid membrane-incorporated porphyrin derivative aqueous solutions and their photodynamic activities, PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 18(2), 459-466, 20190201
  55. High photodynamic activities of water-soluble inclusion complexes of 5,15-diazaporphyrins in cyclodextrin, Organic & Biomolecular Chemistry, 17(12), 3141-3149, 20190227
  56. Insulin sensor cells for the analysis of insulin secretion responses in single living pancreatic beta cells, Analyst, 144, 3765-3772, 2019
  57. Arginine-mediated dissociation of single cells and cell sheets from a polystyrene culture dish, Biosci. Biotechnol. Biochem., 1-4, 20190904
  58. Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy, Genes Environ., 41, 14, 2019
  59. Aggregation - Induced Emission and Retention of Crystal Chiral Information of Tetraphenylethylene Incorporated by Polysaccharides in Water, ChemPhotoChem, 4, 577-581, 2020
  60. Application of peptides with an affinity for phospholipid membranes during the automated purification of extracellular vesicles., Sci. Rep., 10(1), 18718-18718, 2020
  61. Engineering Cofactor Specificity of a Thermostable Phosphite Dehydrogenase for a Highly Efficient and Robust NADPH Regeneration System, Front. Bioeng. Biotechnol., 2021,04,01

Publications such as books

  1. 2011, Embryonic Stem Cells - Basic Biology to Bioengineering, Functional Control of Target Single Cells in ES Cell Clusters and Their Differentiated Cells by Femtoinjection, INTECH Open Access Publisher, 2011, Hideaki Matsuoka, Mikako Saito, Hisakage Funabashi, 978-953-307-278-4, 149-170
  2. 2010, Biofunctional analysis in single-living cells using injection techniques, Chemical Sensors, 26(3), 2010, FUNABASHI Hisakage;SAITO Mikako;MATSUOKA Hideaki, 120-126
  3. 2009, Chapter 17, Nucleic Acid Engineering: towards synthetic biology, Systems Biology and Synthetic Biology, John Wiley & Sons, Inc, 2009, Wenlong Cheng, Liang Ding, Hisakage Funabashi, Nokyoung Park, Soong Ho Um, Jianfeng Xu, Dan Luo, 549-576

Invited Lecture, Oral Presentation, Poster Presentation

  1. A Protein-Based Artificial Receptor for Insulin Sensing, Hisakage Funabashi and Hajime Shigeto, Pacific Rim Meeting on Electrochemical and Solid-state Science (PRiME 2020) Joint international meeting: 238th Meeting of The Electrochemical Society (ECS), 2020 Fall Meeting of The Electrochemical Society of Japan (ECSJ), and 2020 Fall Meeting of The Korean Electrochemical Society (KECS), 2020/10/04, With Invitation, English
  2. Femtoinjection Technique for The Analysis and Regulation of Target-single-living ES cells, Hisakage Funabashi, Mikako Saito, Hideaki Matsuoka, 5th IEEE International Conference on Nano/Molecular Medicine and Engineering, 2011/11/10, With Invitation, English, International Convention Center (ICC), Jeju, Korea
  3. Nucleic Acid Engineering, Hisakage Funabashi, Dan Luo, 2009/08/07, With Invitation, Japanese
  4. Development of insulin-sensing protein probe and its application for the analysis of living cellular response, Hisakage Funabashi, Hajime Shigeto, Biosensors 2020/2021, 31st Anniversary World Congress on Biosensors,, 2021/07/27, Without Invitation, English, Elsevier / Biosensors & Bioelectronics, Live and On-demand
  5. Electrochemical Detection of Insulin with a Novel Protein-Based Artificial Receptor, Yusuke Murai, Takeshi Ikeda, Takenori Isida, Ryuichi Hirota, Akio Kuroda, Hisakage Funabashi, Pacific Rim Meeting on Electrochemical and Solid-state Science (PRiME 2020) Joint international meeting: 238th Meeting of The Electrochemical Society (ECS), 2020 Fall Meeting of The Electrochemical Society of Japan (ECSJ), and 2020 Fall Meeting of The Korean Electrochemical Society (KECS), 2020/10/04, Without Invitation, English
  6. Detection of Specific Nucleic Acids Utilizing DNA Nano-Tweezers Structures, Hisakage Funabashi, Keisuke Yoshinaga, Hajime Shigeto, Keisuke Nakatsuka, Akio Kuroda, Pacific Rim Meeting on Electrochemical and Solid-state Science (PRiME 2016) Joint international meeting: 230th Meeting of The Electrochemical Society (ECS), 2016 Fall Meeting of The Electrochemical Society of Japan (ECSJ), and 2016 Fall Meeting of The Korean Electrochemical Society (KECS), 2016/10/05, English, Honolulu, Hawaii, USA
  7. Imaging analysis of specific mRNA expression based on the FRET-based DNA nano-tweezers technique, Hisakage Funabashi, Hajime Shigeto, Keisuke Nakatsuka, and Akio Kuroda, The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015), 2015/12/18, English, Honolulu, Hawaii, USA
  8. Detection of Inter/intra Cellular Response by Biofunctional Material-based Sensors, Hisakage Funabashi, 2nd Hiroshima International Symposium on Sustainable Sciences, 2014/11/06, English, Hiroshima University, Higashihiroshima, Japan
  9. Development of FRET-based DNA Nano-tweezers for Glucose Transporter Gene Expression Analysis, Hajime Shigeto, Keisuke Nakatsuka, Akio Kuroda, Hisakage Funabashi, 2nd Hiroshima International Symposium on Sustainable Sciences, 2014/11/06, English, Hiroshima University, Higashihiroshima, Japan
  10. Development of the split G-quadruplex based DNA nano-tweezers for target microRNA detection, Keisuke Nakatsuka, Akio Kuroda, Hisakage Funabashi, 2nd Hiroshima International Symposium on Sustainable Sciences, 2014/11/06, English, Hiroshima University, Higashihiroshima, Japan

External Funds

Acceptance Results of Competitive Funds

  1. 2021
  2. 2018
  3. KAKENHI, 2014, 2016
  4. KAKENHI, 2013, 2015
  5. KAKENHI, 2013, 2015
  6. KAKENHI, 2011, 2014
  7. KAKENHI, 2011, 2014
  8. KAKENHI, Development of a platform for a novel proteome analysis in a living cell utilizing proteoinjection, 2009, 2010

Social Activities

History as Committee Members

  1. Biosensors and Bioelectronics, Associate editor, 2019/10, ELSEVIER
  2. Journal of Bioscience and Bioengineering, Editorial Board, 2017/06, 2021/05